Restriction map for pF1KSDA0554
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AflIII      AgeI        AhdI        Alw44I      
AocI        Aor51HI     ApaI        ApaLI       AseI        
AsnI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BbeI        BbrPI       
BcgI        BciVI       BcnI        BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaHI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsoBI       Bsp1407I    BspDI       BspEI       BspLU11I    
BsrDI       BsrGI       BssSI       BstAPI      BstBI       
BstEII      BstPI       BstXI       BstYI       Bsu36I      
CelII       ClaI        CpoI        DraI        DraII       
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FseI        FspI        HaeII       
Hin1I       HpaI        KasI        KpnI        KspI        
MflI        MluI        MroI        MslI        NarI        
NciI        NcoI        NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       StyI        SwaI        Tth111I     XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AlwNI         319  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AscI         1476  GGCGCGCC             GG^CGCGCC
Asp700I      1700  GAACGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI          16  GAGCTC               G[AT]GC[AT]^C
BalI         1604  TGGCCA               TGG^CCA
BanI           10  GGCACC               G^G[CT][GA]CC
BbvCI        1438  GCTGAGG              CCTCAGC(-5/-2)
BclI          777  TGATCA               T^GATCA
BfaI          407  CTAG                 C^TAG
BsaBI         723  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI        1194  ACCGGA               [AT]^CCGG[AT]
BsiHKAI        16  GAGCTC               G[AT]GC[AT]^C
BsmI         1769  GCATTC               GAATGC(1/-1)
BspHI        1121  TCATGA               T^CATGA
BspMI         717  GCAGGT               ACCTGC(4/8)
BsrBI        1488  GAGCGG               CCGCTC(-3/-3)
BssHII       1477  GCGCGC               G^CGCGC
Bst1107I      198  GTATAC               GTA^TAC
BstZ17I       198  GTATAC               GTA^TAC
DraIII         51  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI          481  GACATCAATGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1607  CCACGG               C^C[GA][CT]GG
EarI         1789  GAAGAG               CTCTTC(1/4)
EcoNI        1648  CCTGCCATAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI         1766  CCCGC                CCCGC(4/6)
FbaI          777  TGATCA               T^GATCA
HgaI          475  GACGC                GACGC(5/10)
HincII       1522  GTCAAC               GT[CT]^[GA]AC
HindII       1522  GTCAAC               GT[CT]^[GA]AC
Ksp632I      1789  GAAGAG               CTCTTC(1/4)
MaeI          407  CTAG                 C^TAG
MamI          723  GATCCCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          773  CAATTG               C^AATTG
MluNI        1604  TGGCCA               TGG^CCA
MscI         1604  TGGCCA               TGG^CCA
MspA1I        796  CAGCTG               C[AC]G^C[GT]G
MunI          773  CAATTG               C^AATTG
PflMI         727  CCAATCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         1240  CTGCAG               CTGCA^G
PvuII         796  CAGCTG               CAG^CTG
RcaI         1121  TCATGA               T^CATGA
SacI           16  GAGCTC               GAGCT^C
ScaI          458  AGTACT               AGT^ACT
SfcI         1240  CTGCAG               C^T[GA][CT]AG
SspI          608  AATATT               AAT^ATT
StuI         1427  AGGCCT               AGG^CCT
Van91I        727  CCAATCATTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI         1700  GAACGATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccI          198  GTATAC               GT^[AC][GT]AC
             1321  GTCTAC              
AccII         427  CGCG                 CG^CG
             1478  CGCG                
AvaII        1539  GGACC                G^G[AT]CC
             1763  GGACC               
BbsI          693  GAAGAC               GAAGAC(2/6)
             1744  GAAGAC              
BglI         1429  GCCTGGCTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1550  GCCCAGATGGC         
BpmI          580  CTCCAG               CTGGAG(16/14)
              836  CTGGAG              
BpuAI         693  GAAGAC               GAAGAC(2/6)
             1744  GAAGAC              
BsaI         1346  GAGACC               GGTCTC(1/5)
             1411  GAGACC              
BsaJI        1535  CCCAGG               C^C[ACGT][ACGT]GG
             1607  CCACGG              
BsrFI        1451  GCCGGC               [GA]^CCGG[CT]
             1481  GCCGGC              
BsrI         1071  CCAGT                ACTGG(1/-1)
             1355  CCAGT               
BstUI         427  CGCG                 CG^CG
             1478  CGCG                
Cfr10I       1451  GCCGGC               [GA]^CCGG[CT]
             1481  GCCGGC              
MseI         1000  TTAA                 T^TAA
             1261  TTAA                
MvnI          427  CGCG                 CG^CG
             1478  CGCG                
NaeI         1451  GCCGGC               GCC^GGC
             1481  GCCGGC              
NdeI          698  CATATG               CA^TATG
             1811  CATATG              
NgoMIV       1451  GCCGGC               G^CCGGC
             1481  GCCGGC              
PleI          685  GAGTC                GAGTC(4/5)
             1578  GAGTC               
SfaNI        1297  GATGC                GCATC(5/9)
              283  GCATC               
SmlI           73  CTTGAG               C^T[CT][GA]AG
             1177  CTCAAG              
Tru9I        1000  TTAA                 T^TAA
             1261  TTAA                
Tsp45I        490  GTCAC                ^GT[GC]AC
              536  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   57 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        BanII       BbvI        BmyI        BseRI       
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BssKI       BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       EaeI        EcoRII      
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MnlI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       RsaI        Sau3AI      Sau96I      
ScrFI       TaqI        TfiI        TseI        Tsp509I     
TspRI       TthHB8I     
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