Restriction map for pF1KSDA0544
 (Linear) (Six-base) MAPSORT of: KIAA0544.seq  Check: 5852  from: 1  to: 1656



KIAA0544.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1605    1656 
   Size:       1605      51
 
AccI GT'mk_AC

Cuts at:      0     443    1656 
   Size:        443    1213
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167    1005    1656 
   Size:        167     838     651

  Fragments arranged by size: 

                 838     651     167
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1266    1656 
   Size:       1266     390
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1044    1656 
   Size:       1044     612
 
ApaI G_GGCC'C

Cuts at:      0     966    1628    1656 
   Size:        966     662      28

  Fragments arranged by size: 

                 966     662      28
 
ApoI r'AATT_y

Cuts at:      0     127    1656 
   Size:        127    1529
 
AvaI C'yCGr_G

Cuts at:      0     341    1174    1532    1573    1656 
   Size:        341     833     358      41      83

  Fragments arranged by size: 

                 833     358     341      83      41
 
BanII G_rGCy'C

Cuts at:      0       7     239     460     966    1005    1163    1628    1656 
   Size:          7     232     221     506      39     158     465      28

  Fragments arranged by size: 

                 506     465     232     221     158      39      28       7
            
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     250    1367    1656 
   Size:        250    1117     289

  Fragments arranged by size: 

                1117     289     250
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     361     395    1656 
   Size:        361      34    1261

  Fragments arranged by size: 

                1261     361      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     388    1102    1328    1656 
   Size:        388     714     226     328

  Fragments arranged by size: 

                 714     388     328     226
 
BglII A'GATC_T

Cuts at:      0     755    1656 
   Size:        755     901
 
Bme1580I G_kGCm'C

Cuts at:      0     966    1628    1656 
   Size:        966     662      28

  Fragments arranged by size: 

                 966     662      28
 
BmgBI CAC'GTC

Cuts at:      0    1419    1656 
   Size:       1419     237
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1447    1656 
   Size:       1447     209
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1182    1425    1656 
   Size:       1182     243     231

  Fragments arranged by size: 

                1182     243     231
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379    1066    1656 
   Size:        379     687     590

  Fragments arranged by size: 

                 687     590     379
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     469     781    1656 
   Size:        469     312     875

  Fragments arranged by size: 

                 875     469     312
 
BsaAI yAC'GTr

Cuts at:      0     654    1656 
   Size:        654    1002
 
BsaHI Gr'CG_yC

Cuts at:      0     374    1424    1656 
   Size:        374    1050     232

  Fragments arranged by size: 

                1050     374     232
 
BsaWI w'CCGG_w

Cuts at:      0      85    1656 
   Size:         85    1571
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     614     644    1081    1111    1656 
   Size:        614      30     437      30     545

  Fragments arranged by size: 

                 614     545     437      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     134     433    1414    1656 
   Size:        134     299     981     242

  Fragments arranged by size: 

                 981     299     242     134
 
BsiHKAI G_wGCw'C

Cuts at:      0     350     659    1061    1580    1656 
   Size:        350     309     402     519      76

  Fragments arranged by size: 

                 519     402     350     309      76
 
Bsp1286I G_dGCh'C

Cuts at:      0       7     239     350     460     659     966    1005    1061 
   Size:          7     232     111     110     199     307      39      56

Cuts at:   1061    1163    1580    1628    1656 
   Size:        102     417      48      28

  Fragments arranged by size: 

                 417     307     232     199     111     110     102      56
                  48      39      28       7
 
BspEI T'CCGG_A

Cuts at:      0      85    1656 
   Size:         85    1571
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     860    1605    1619    1656 
   Size:        860     745      14      37

  Fragments arranged by size: 

                 860     745      37      14
 
BsrFI r'CCGG_y

Cuts at:      0     947    1656 
   Size:        947     709
 
BssSI C'ACGA_G

Cuts at:      0     232    1656 
   Size:        232    1424
 
BstEII G'GTnAC_C

Cuts at:      0     151    1656 
   Size:        151    1505
 
BstYI r'GATC_y

Cuts at:      0     174     755     974    1656 
   Size:        174     581     219     682

  Fragments arranged by size: 

                 682     581     219     174
 
BstZ17I GTA'TAC

Cuts at:      0     444    1656 
   Size:        444    1212
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0       9    1656 
   Size:          9    1647
 
DraI TTT'AAA

Cuts at:      0     710    1074    1656 
   Size:        710     364     582

  Fragments arranged by size: 

                 710     582     364
 
DraIII CAC_nnn'GTG

Cuts at:      0    1441    1656 
   Size:       1441     215
 
EaeI y'GGCC_r

Cuts at:      0     389     642    1103    1208    1584    1656 
   Size:        389     253     461     105     376      72

  Fragments arranged by size: 

                 461     389     376     253     105      72
 
EarI CTCTTCn'nnn_

Cuts at:      0    1385    1490    1656 
   Size:       1385     105     166

  Fragments arranged by size: 

                1385     166     105
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167     379    1005    1066    1656 
   Size:        167     212     626      61     590

  Fragments arranged by size: 

                 626     590     212     167      61
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     748    1270    1656 
   Size:        748     522     386

  Fragments arranged by size: 

                 748     522     386
 
EcoO109I rG'GnC_Cy

Cuts at:      0     963    1347    1529    1624    1656 
   Size:        963     384     182      95      32

  Fragments arranged by size: 

                 963     384     182      95      32
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     552    1656 
   Size:        552    1104
 
HaeII r_GCGC'y

Cuts at:      0    1183    1656 
   Size:       1183     473
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1045    1077    1252    1284    1495    1498    1527    1530 
   Size:       1045      32     175      32     211       3      29       3

Cuts at:   1530    1621    1653    1656 
   Size:         91      32       3

  Fragments arranged by size: 

                1045     211     175      91      32      32      32      29
                   3       3       3
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     872    1656 
   Size:        872     784
 
MscI TGG'CCA

Cuts at:      0     391     644    1105    1656 
   Size:        391     253     461     551

  Fragments arranged by size: 

                 551     461     391     253
 
MspA1I CmG'CkG

Cuts at:      0    1280    1656 
   Size:       1280     376
 
NaeI GCC'GGC

Cuts at:      0     949    1656 
   Size:        949     707
 
NgoMIV G'CCGG_C

Cuts at:      0     947    1656 
   Size:        947     709
 
PasI CC'CwG_GG

Cuts at:      0    1620    1656 
   Size:       1620      36
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     609    1044    1308    1441    1656 
   Size:        609     435     264     133     215

  Fragments arranged by size: 

                 609     435     264     215     133
 
PfoI T'CCnGG_A

Cuts at:      0    1307    1656 
   Size:       1307     349
 
PmlI CAC'GTG

Cuts at:      0     654    1656 
   Size:        654    1002
 
PpuMI rG'GwC_Cy

Cuts at:      0    1529    1656 
   Size:       1529     127
 
PspOMI G'GGCC_C

Cuts at:      0     962    1624    1656 
   Size:        962     662      32

  Fragments arranged by size: 

                 962     662      32
 
PspXI vC'TCGA_Gb

Cuts at:      0    1174    1656 
   Size:       1174     482
 
PstI C_TGCA'G

Cuts at:      0     752     974    1562    1656 
   Size:        752     222     588      94

  Fragments arranged by size: 

                 752     588     222      94
 
PvuII CAG'CTG

Cuts at:      0    1280    1656 
   Size:       1280     376
 
SanDI GG'GwC_CC

Cuts at:      0    1529    1656 
   Size:       1529     127
 
SbfI CC_TGCA'GG

Cuts at:      0     752    1562    1656 
   Size:        752     810      94

  Fragments arranged by size: 

                 810     752      94
 
SfcI C'TryA_G

Cuts at:      0     351     466     748     970    1558    1656 
   Size:        351     115     282     222     588      98

  Fragments arranged by size: 

                 588     351     282     222     115      98
 
SmaI CCC'GGG

Cuts at:      0     343    1656 
   Size:        343    1313
 
SmlI C'TyrA_G

Cuts at:      0    1174    1656 
   Size:       1174     482
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1429    1656 
   Size:       1429     227
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     614     646    1081    1113    1656 
   Size:        614      32     435      32     543

  Fragments arranged by size: 

                 614     543     435      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     733    1141    1656 
   Size:        733     408     515

  Fragments arranged by size: 

                 733     515     408
 
XhoI C'TCGA_G

Cuts at:      0    1174    1656 
   Size:       1174     482
 
XmaI C'CCGG_G

Cuts at:      0     341    1656 
   Size:        341    1315
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AhdI    AlwNI     ApaI     ApoI     AvaI
    BanII     BbsI     BcgI     BglI    BglII Bme1580I    BmgBI     BmrI
     BplI     BpmI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
  BsiHKAI Bsp1286I    BspEI    BspMI    BsrFI    BssSI   BstEII    BstYI
  BstZ17I    BtgZI     DraI   DraIII     EaeI     EarI  Eco57MI    EcoNI
 EcoO109I     FalI    HaeII    Hin4I     MmeI     MscI   MspA1I     NaeI
   NgoMIV     PasI    PflMI     PfoI     PmlI    PpuMI   PspOMI    PspXI
     PstI    PvuII    SanDI     SbfI     SfcI     SmaI     SmlI    TaqII
     TstI  Tth111I     XhoI     XmaI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AleI
     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI     BlpI
     BmtI   Bpu10I    BpuEI    BsaBI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrGI   BssHII   BstAPI
    BstBI    BstXI   Bsu36I     BtgI     BtsI     ClaI    CspCI     DrdI
     EagI     EciI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI     NspI
     PacI     PciI     PmeI     PpiI    PshAI     PsiI     PsrI     PvuI
    RsrII     SacI    SacII     SalI     SapI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     XbaI     XcmI     XmnI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com