Restriction map for pF1KSDA0537
##################################
# NO CUT ENZYMES (  106 enzymes) #
##################################

AatII       AccIII      AcsI        AflII       AflIII      
AgeI        AocI        ApoI        AscI        AseI        
AsnI        Asp700I     AspI        AviII       AvrII       
BbrPI       BbvCI       BciVI       BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BseAI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstXI       
BstZ17I     Bsu36I      CelII       ClaI        CpoI        
DraI        DrdI        EagI        EclXI       Eco52I      
Eco81I      EcoRI       EcoRV       FseI        FspI        
HincII      HindII      HindIII     HpaI        KspI        
MfeI        MluI        MroI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NspI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshBI       
RcaI        RsrII       SacI        SacII       SalI        
SapI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       StuI        SwaI        
Tth111I     XbaI        XcmI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   57 enzymes) #
###################################

Acc65I       1880  GGTACC               G^GTACC
AccI         1945  GTCTAC               GT^[AC][GT]AC
AccII          15  CGCG                 CG^CG
AclI          630  AACGTT               AA^CGTT
AhdI          964  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I        496  GTGCAC               G^TGCAC
Aor51HI      1956  AGCGCT               AGC^GCT
ApaI          710  GGGCCC               GGGCC^C
ApaLI         496  GTGCAC               G^TGCAC
Asp718I      1880  GGTACC               G^GTACC
AspEI         964  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI         1055  TGGCCA               TGG^CCA
BamHI        1196  GGATCC               G^GATCC
BbeI           54  GGCGCC               GGCGC^C
BbsI         1458  GTCTTC               GAAGAC(2/6)
BcgI         1764  GCAGGAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          386  TGATCA               T^GATCA
BfaI         1084  CTAG                 C^TAG
BglII        1964  AGATCT               A^GATCT
BpuAI        1458  GTCTTC               GAAGAC(2/6)
BsaWI        1883  ACCGGA               [AT]^CCGG[AT]
BsgI         1305  GTGCAG               GTGCAG(16/14)
BsrDI         897  CATTGC               GCAATG(2/0)
BstEII       1533  GGTAACC              G^GT[ACGT]ACC
BstPI        1533  GGTAACC              G^GT[ACGT]ACC
BstUI          15  CGCG                 CG^CG
DraIII        118  CACGGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         1604  CCATGG               C^C[GA][CT]GG
Eam1105I      964  GACTCTGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI         1437  CTCTTC               CTCTTC(1/4)
Eco47III     1956  AGCGCT               AGC^GCT
EcoO65I      1533  GGTAACC              G^GT[ACGT]ACC
EcoT22I       653  ATGCAT               ATGCA^T
FbaI          386  TGATCA               T^GATCA
KasI           54  GGCGCC               G^GCGCC
KpnI         1880  GGTACC               GGTAC^C
Ksp632I      1437  CTCTTC               CTCTTC(1/4)
MaeI         1084  CTAG                 C^TAG
MaeII         631  ACGT                 A^CGT
MluNI        1055  TGGCCA               TGG^CCA
MscI         1055  TGGCCA               TGG^CCA
MslI         1920  CACAGACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           15  CGCG                 CG^CG
NarI           54  GGCGCC               GG^CGCC
NcoI         1604  CCATGG               C^CATGG
NsiI          653  ATGCAT               ATGCA^T
PaeR7I        836  CTCGAG               C^TCGAG
PflMI         902  CCAACCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1938  GACTCAGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      630  AACGTT               AA^CGTT
PvuI          873  CGATCG               CGAT^CG
SfoI           54  GGCGCC               GGC^GCC
SmlI          836  CTCGAG               C^T[CT][GA]AG
SspI          571  AATATT               AAT^ATT
Tsp509I       270  AATT                 ^AATT
Van91I        902  CCAACCACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          836  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AcyI           54  GGCGCC               G[GA]^CG[CT]C
              443  GACGCC              
AspHI         496  GTGCAC               G[AT]GC[AT]^C
             1318  GTGCTC              
AvaI           68  CCCGAG               C^[CT]CG[GA]G
              836  CTCGAG              
BsaBI         309  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              381  GATTGTGATC          
BsaHI          54  GGCGCC               G[GA]^CG[CT]C
              443  GACGCC              
BseRI        1321  CTCCTC               GAGGAG(10/8)
             1915  CTCCTC              
BsiEI         873  CGATCG               CG[GA][CT]^CG
             1096  CGGTCG              
BsiHKAI       496  GTGCAC               G[AT]GC[AT]^C
             1318  GTGCTC              
BsmBI         486  CGTCTC               CGTCTC(1/5)
             1803  CGTCTC              
BsoBI          68  CCCGAG               C^[CT]CG[GA]G
              836  CTCGAG              
BsrBI         436  GAGCGG               CCGCTC(-3/-3)
             1087  GAGCGG              
BsrFI        1853  ACCGGC               [GA]^CCGG[CT]
             1889  ACCGGC              
Cfr10I       1853  ACCGGC               [GA]^CCGG[CT]
             1889  ACCGGC              
EcoNI         682  CCTTACCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1553  CCTGCCGGAGG         
EcoT14I      1190  CCAAGG               C^C[AT][AT]GG
             1604  CCATGG              
HaeII          54  GGCGCC               [GA]GCGC^[CT]
             1956  AGCGCT              
HgaI          443  GACGC                GACGC(5/10)
             1802  GCGTC               
Hin1I          54  GGCGCC               G[GA]^CG[CT]C
              443  GACGCC              
MamI          309  GATTGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              381  GATTGTGATC          
MseI          272  TTAA                 T^TAA
              575  TTAA                
PvuII         705  CAGCTG               CAG^CTG
             1797  CAGCTG              
StyI         1190  CCAAGG               C^C[AT][AT]GG
             1604  CCATGG              
Tru9I         272  TTAA                 T^TAA
              575  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaI        BsaJI       BsiYI       
BslI        BsmAI       BsmFI       Bsp1286I    BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       HphI        ItaI        MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
TaqI        TfiI        TseI        Tsp45I      TspRI       
TthHB8I     XhoII       
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