################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## AatII Acc65I AccI AccII AclI AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbvCI BcgI BclI BglI BlnI BsiEI BsiWI BsmBI BspDI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FbaI FseI FspI HgaI HpaI KpnI KspI MluI MvnI NaeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccIII 2368 TCCGGA T^CCGGA AcyI 1645 GGCGCC G[GA]^CG[CT]C AflIII 1131 ACACGT A^C[GA][CT]GT AlwI 1107 GATCC GGATC(4/5) Asp700I 304 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 2325 CTCGGG C^[CT]CG[GA]G AvaII 3004 GGACC G^G[AT]CC BbeI 1645 GGCGCC GGCGC^C BciVI 3131 GTATCC GTATCC(6/5) BglII 2145 AGATCT A^GATCT BlpI 940 GCTGAGC GC^T[ACGT]AGC Bpu1102I 940 GCTGAGC GC^T[ACGT]AGC BsaBI 1345 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 1645 GGCGCC G[GA]^CG[CT]C BsaI 1093 GAGACC GGTCTC(1/5) BsaWI 2368 TCCGGA [AT]^CCGG[AT] BseAI 2368 TCCGGA T^CCGGA BsoBI 2325 CTCGGG C^[CT]CG[GA]G Bsp1407I 3056 TGTACA T^GTACA BspEI 2368 TCCGGA T^CCGGA BspMI 2947 GCAGGT ACCTGC(4/8) BsrGI 3056 TGTACA T^GTACA BssSI 2378 CTCGTG CACGAG(-5/-1) BstXI 1656 CCAGAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 2145 AGATCT [GA]^GATC[CT] CelII 940 GCTGAGC GC^T[ACGT]AGC CfoI 1646 GCGC GCG^C DraII 3003 GGGACCT [GA]G^G[ACGT]CC[CT] DraIII 1180 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 104 CCATGG C^C[GA][CT]GG EcoO109I 3003 GGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1423 GAATTC G^AATTC HaeII 1645 GGCGCC [GA]GCGC^[CT] HhaI 1646 GCGC GCG^C Hin1I 1645 GGCGCC G[GA]^CG[CT]C HinP1I 1646 GCGC G^CGC HincII 151 GTCAAC GT[CT]^[GA]AC HindII 151 GTCAAC GT[CT]^[GA]AC KasI 1645 GGCGCC G^GCGCC MamI 1345 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 2145 AGATCT [GA]^GATC[CT] MroI 2368 TCCGGA T^CCGGA NarI 1645 GGCGCC GG^CGCC NcoI 104 CCATGG C^CATGG NdeI 3032 CATATG CA^TATG NspI 1797 GCATGC [GA]CATG^[CT] PpuMI 3003 GGGACCT [GA]G^G[AT]CC[CT] SfoI 1645 GGCGCC GGC^GCC SphI 1797 GCATGC GCATG^C SspBI 3056 TGTACA T^GTACA SwaI 443 ATTTAAAT ATTT^AAAT XbaI 2070 TCTAGA T^CTAGA XhoII 2145 AGATCT [GA]^GATC[CT] XmnI 304 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AflII 1845 CTTAAG C^TTAAG 2296 CTTAAG BalI 2196 TGGCCA TGG^CCA 2466 TGGCCA BcnI 1243 CCGGG CC^[GC]GG 3084 CCCGG BfrI 1845 CTTAAG C^TTAAG 2296 CTTAAG BspHI 284 TCATGA T^CATGA 2726 TCATGA BstAPI 1717 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1720 GCAGCTGCTGC DraI 444 TTTAAA TTT^AAA 468 TTTAAA EcoT22I 1213 ATGCAT ATGCA^T 2216 ATGCAT MfeI 926 CAATTG C^AATTG 1310 CAATTG MluNI 2196 TGGCCA TGG^CCA 2466 TGGCCA MscI 2196 TGGCCA TGG^CCA 2466 TGGCCA MunI 926 CAATTG C^AATTG 1310 CAATTG NciI 1243 CCGGG CC^[GC]GG 3084 CCCGG NsiI 1213 ATGCAT ATGCA^T 2216 ATGCAT RcaI 284 TCATGA T^CATGA 2726 TCATGA StuI 1078 AGGCCT AGG^CCT 2105 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AspHI BanI BanII BbrPI BbsI BbvI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EarI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PmaCI PmlI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I