(Circular) (Six-base) MAPSORT of: pF1KSDA0535.seq Check: 1473 from: 1 to: 6280 pF1KSDA0535 6280 bp With 182 enzymes: SgfI * June 20, 2008 10:33 .. AccI GT'mk_AC Cuts at: 3316 3316 Size: 6280 Acc65I G'GTAC_C Cuts at: 3294 3294 Size: 6280 AclI AA'CG_TT Cuts at: 6120 6120 Size: 6280 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 161 272 860 2864 2959 4103 4535 5284 5552 Size: 111 588 2004 95 1144 432 749 268 Cuts at: 5552 161 Size: 889 Fragments arranged by size: 2004 1144 889 749 588 432 268 111 95 AflII C'TTAA_G Cuts at: 1971 2422 1971 Size: 451 5829 AflIII A'CryG_T Cuts at: 1257 3630 4736 5713 1257 Size: 2373 1106 977 1824 Fragments arranged by size: 2373 1824 1106 977 AgeI A'CCGG_T Cuts at: 4668 4668 Size: 6280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 639 1070 639 Size: 431 5849 AlwNI CAG_nnn'CTG Cuts at: 139 1849 1852 1920 2638 3583 5152 139 Size: 1710 3 68 718 945 1569 1267 Fragments arranged by size: 1710 1569 1267 945 718 68 3 ApaLI G'TGCA_C Cuts at: 5050 5050 Size: 6280 ApoI r'AATT_y Cuts at: 529 567 850 1029 1328 1549 1744 2202 3147 Size: 38 283 179 299 221 195 458 945 Cuts at: 3147 3282 5633 5889 529 Size: 135 2351 256 920 Fragments arranged by size: 2351 945 920 458 299 283 256 221 195 179 135 38 AseI AT'TA_AT Cuts at: 20 20 Size: 6280 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 6280 AvaI C'yCGr_G Cuts at: 2451 3298 2451 Size: 847 5433 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 471 504 471 Size: 33 6247 BamHI G'GATC_C Cuts at: 3303 3303 Size: 6280 BanI G'GyrC_C Cuts at: 180 1298 1771 3294 3708 3956 3991 180 Size: 1118 473 1523 414 248 35 2469 Fragments arranged by size: 2469 1523 1118 473 414 248 35 BanII G_rGCy'C Cuts at: 209 500 1271 1789 2194 3292 4322 209 Size: 291 771 518 405 1098 1030 2167 Fragments arranged by size: 2167 1098 1030 771 518 405 291 BbeI G_GCGC'C Cuts at: 1775 3712 3960 1775 Size: 1937 248 4095 Fragments arranged by size: 4095 1937 248 BbsI GAAGACnn'nnnn_ Cuts at: 347 427 479 1179 1835 2164 2561 347 Size: 80 52 700 656 329 397 4066 Fragments arranged by size: 4066 700 656 397 329 80 52 BciVI GTATCCnnnnn_n' Cuts at: 3268 4176 4939 6280 3268 Size: 908 763 1341 3268 Fragments arranged by size: 3268 1341 908 763 BclI T'GATC_A Cuts at: 3798 3798 Size: 6280 BfrBI ATG'CAT Cuts at: 1341 2344 1341 Size: 1003 5277 BglII A'GATC_T Cuts at: 2271 3793 5832 2271 Size: 1522 2039 2719 Fragments arranged by size: 2719 2039 1522 BlpI GC'TnA_GC Cuts at: 1067 3390 1067 Size: 2323 3957 Bme1580I G_kGCm'C Cuts at: 1231 1303 2459 3903 3996 5054 1231 Size: 72 1156 1444 93 1058 2457 Fragments arranged by size: 2457 1444 1156 1058 93 72 BmrI ACTGGGnnnn_n' Cuts at: 1508 2179 2885 3490 3571 3906 6052 1508 Size: 671 706 605 81 335 2146 1736 Fragments arranged by size: 2146 1736 706 671 605 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2710 4645 2710 Size: 1935 4345 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 444 1063 1141 2246 444 Size: 619 78 1105 4478 Fragments arranged by size: 4478 1105 619 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1339 3454 4333 4827 5125 1339 Size: 2115 879 494 298 2494 Fragments arranged by size: 2494 2115 879 494 298 BsaI GGTCTCn'nnnn_ Cuts at: 1213 5984 1213 Size: 4771 1509 BsaAI yAC'GTr Cuts at: 1260 2328 2460 3270 3631 1260 Size: 1068 132 810 361 3909 Fragments arranged by size: 3909 1068 810 361 132 BsaBI GATnn'nnATC Cuts at: 6 1475 3792 5831 6 Size: 1469 2317 2039 455 Fragments arranged by size: 2317 2039 1469 455 BsaHI Gr'CG_yC Cuts at: 1772 3709 3957 4661 6157 1772 Size: 1937 248 704 1496 1895 Fragments arranged by size: 1937 1895 1496 704 248 BsaWI w'CCGG_w Cuts at: 2494 3467 3988 4668 4942 5089 5500 2494 Size: 973 521 680 274 147 411 3274 Fragments arranged by size: 3274 973 680 521 411 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 727 757 1210 1240 1885 1915 2410 2440 727 Size: 30 453 30 645 30 495 30 4567 Fragments arranged by size: 4567 645 495 453 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 678 976 2179 2182 3034 3037 678 Size: 298 1203 3 852 3 3921 Fragments arranged by size: 3921 1203 852 298 3 3 BseYI C'CCAG_C Cuts at: 731 938 2120 2754 3703 4461 5040 5536 731 Size: 207 1182 634 949 758 579 496 1475 Fragments arranged by size: 1475 1182 949 758 634 579 496 207 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 431 740 1787 1991 431 Size: 309 1047 204 4720 Fragments arranged by size: 4720 1047 309 204 BsiEI CG_ry'CG Cuts at: 104 3820 3866 5076 104 Size: 3716 46 1210 1308 Fragments arranged by size: 3716 1308 1210 46 BsiHKAI G_wGCw'C Cuts at: 209 500 1127 1355 2194 3292 4070 4260 5054 Size: 291 627 228 839 1098 778 190 794 Cuts at: 5054 209 Size: 1435 Fragments arranged by size: 1435 1098 839 794 778 627 291 228 190 BsmI GAATG_Cn' Cuts at: 912 2072 2089 3220 912 Size: 1160 17 1131 3972 Fragments arranged by size: 3972 1160 1131 17 Bsp1286I G_dGCh'C Cuts at: 209 500 1127 1231 1271 1303 1355 1789 2194 Size: 291 627 104 40 32 52 434 405 Cuts at: 2194 2459 3292 3903 3996 4070 4260 4322 5054 Size: 265 833 611 93 74 190 62 732 Cuts at: 5054 209 Size: 1435 Fragments arranged by size: 1435 833 732 627 611 434 405 291 265 190 104 93 74 62 52 40 32 BspEI T'CCGG_A Cuts at: 2494 5500 2494 Size: 3006 3274 BspHI T'CATG_A Cuts at: 410 2852 5456 410 Size: 2442 2604 1234 Fragments arranged by size: 2604 2442 1234 BspMI ACCTGCnnnn'nnnn_ Cuts at: 3064 3328 3542 3844 4225 3064 Size: 264 214 302 381 5119 Fragments arranged by size: 5119 381 302 264 214 BsrBI CCG'CTC Cuts at: 50 4570 4624 6110 6275 50 Size: 4520 54 1486 165 55 Fragments arranged by size: 4520 1486 165 55 54 BsrDI GCAATG_nn' Cuts at: 939 1466 2754 4190 939 Size: 527 1288 1436 3029 Fragments arranged by size: 3029 1436 1288 527 BsrFI r'CCGG_y Cuts at: 4276 4457 4668 4276 Size: 181 211 5888 Fragments arranged by size: 5888 211 181 BsrGI T'GTAC_A Cuts at: 3182 3182 Size: 6280 BssSI C'ACGA_G Cuts at: 2504 4549 4909 2504 Size: 2045 360 3875 Fragments arranged by size: 3875 2045 360 BstAPI GCAn_nnn'nTGC Cuts at: 1849 1852 3655 1849 Size: 3 1803 4474 Fragments arranged by size: 4474 1803 3 BstBI TT'CG_AA Cuts at: 110 110 Size: 6280 BstXI CCAn_nnnn'nTGG Cuts at: 1789 1789 Size: 6280 BstYI r'GATC_y Cuts at: 1 2271 3303 3770 3793 4128 4374 5388 5474 Size: 2270 1032 467 23 335 246 1014 86 Cuts at: 5474 5486 5832 1 Size: 12 346 449 Fragments arranged by size: 2270 1032 1014 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 125 230 125 Size: 105 6175 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 623 2335 2459 4228 4409 4572 623 Size: 1712 124 1769 181 163 2331 Fragments arranged by size: 2331 1769 1712 181 163 124 BtsI GCAGTG_nn' Cuts at: 354 1510 2361 3254 3521 354 Size: 1156 851 893 267 3113 Fragments arranged by size: 3113 1156 893 851 267 DraI TTT'AAA Cuts at: 572 596 3277 572 Size: 24 2681 3575 Fragments arranged by size: 3575 2681 24 DraIII CAC_nnn'GTG Cuts at: 1311 1311 Size: 6280 DrdI GACnn_nn'nnGTC Cuts at: 3984 4844 3984 Size: 860 5420 EaeI y'GGCC_r Cuts at: 1791 2322 2592 3863 4037 4428 4455 5802 1791 Size: 531 270 1271 174 391 27 1347 2269 Fragments arranged by size: 2269 1347 1271 531 391 270 174 27 EagI C'GGCC_G Cuts at: 3863 3863 Size: 6280 EarI CTCTTCn'nnn_ Cuts at: 235 645 992 2055 4301 4511 235 Size: 410 347 1063 2246 210 2004 Fragments arranged by size: 2246 2004 1063 410 347 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 4486 4798 4944 6090 4486 Size: 312 146 1146 4676 Fragments arranged by size: 4676 1146 312 146 EcoICRI GAG'CTC Cuts at: 207 498 2192 3290 207 Size: 291 1694 1098 3197 Fragments arranged by size: 3197 1694 1098 291 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 161 272 444 860 1063 1141 2246 2864 2959 Size: 111 172 416 203 78 1105 618 95 Cuts at: 2959 4103 4535 5284 5552 161 Size: 1144 432 749 268 889 Fragments arranged by size: 1144 1105 889 749 618 432 416 268 203 172 111 95 78 EcoNI CCTnn'n_nnAGG Cuts at: 1015 1766 2579 1015 Size: 751 813 4716 Fragments arranged by size: 4716 813 751 EcoO109I rG'GnC_Cy Cuts at: 3130 3417 3130 Size: 287 5993 EcoRI G'AATT_C Cuts at: 1549 3282 1549 Size: 1733 4547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4297 4297 Size: 6280 FspI TGC'GCA Cuts at: 4059 4059 Size: 6280 HaeII r_GCGC'y Cuts at: 1775 3712 3960 4984 5963 1775 Size: 1937 248 1024 979 2092 Fragments arranged by size: 2092 1937 1024 979 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 466 498 727 759 1885 1917 2792 2824 4120 Size: 32 229 32 1126 32 875 32 1296 Cuts at: 4120 4150 4152 4182 466 Size: 30 2 30 2564 Fragments arranged by size: 2564 1296 1126 875 229 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 279 3317 279 Size: 3038 3242 HindIII A'AGCT_T Cuts at: 2257 2487 2790 2257 Size: 230 303 5747 Fragments arranged by size: 5747 303 230 KasI G'GCGC_C Cuts at: 1771 3708 3956 1771 Size: 1937 248 4095 Fragments arranged by size: 4095 1937 248 KpnI G_GTAC'C Cuts at: 3298 3298 Size: 6280 MfeI C'AATT_G Cuts at: 1052 1436 1052 Size: 384 5896 MluI A'CGCG_T Cuts at: 5713 5713 Size: 6280 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 770 1928 2062 2323 2455 2966 4951 5135 770 Size: 1158 134 261 132 511 1985 184 1915 Fragments arranged by size: 1985 1915 1158 511 261 184 134 132 MscI TGG'CCA Cuts at: 2324 2594 4039 2324 Size: 270 1445 4565 Fragments arranged by size: 4565 1445 270 MslI CAynn'nnrTG Cuts at: 641 1346 2307 2354 2912 2992 3178 4267 4394 Size: 705 961 47 558 80 186 1089 127 Cuts at: 4394 641 Size: 2527 Fragments arranged by size: 2527 1089 961 705 558 186 127 80 47 MspA1I CmG'CkG Cuts at: 250 1447 1849 1877 3389 3703 4063 5078 5323 Size: 1197 402 28 1512 314 360 1015 245 Cuts at: 5323 250 Size: 1207 Fragments arranged by size: 1512 1207 1197 1015 402 360 314 245 28 NaeI GCC'GGC Cuts at: 4459 4459 Size: 6280 NarI GG'CG_CC Cuts at: 1772 3709 3957 1772 Size: 1937 248 4095 Fragments arranged by size: 4095 1937 248 NcoI C'CATG_G Cuts at: 125 230 125 Size: 105 6175 NdeI CA'TA_TG Cuts at: 3159 3159 Size: 6280 NgoMIV G'CCGG_C Cuts at: 4457 4457 Size: 6280 NruI TCG'CGA Cuts at: 4641 4641 Size: 6280 NsiI A_TGCA'T Cuts at: 1343 2346 1343 Size: 1003 5277 NspI r_CATG'y Cuts at: 1927 3331 3831 4740 1927 Size: 1404 500 909 3467 Fragments arranged by size: 3467 1404 909 500 PciI A'CATG_T Cuts at: 4736 4736 Size: 6280 PfoI T'CCnGG_A Cuts at: 200 1994 2223 2698 200 Size: 1794 229 475 3782 Fragments arranged by size: 3782 1794 475 229 PmeI GTTT'AAAC Cuts at: 3277 3277 Size: 6280 PmlI CAC'GTG Cuts at: 1260 2328 2460 1260 Size: 1068 132 5080 Fragments arranged by size: 5080 1068 132 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5419 5451 5419 Size: 32 6248 PpuMI rG'GwC_Cy Cuts at: 3130 3130 Size: 6280 PstI C_TGCA'G Cuts at: 1064 1446 1882 3325 4010 1064 Size: 382 436 1443 685 3334 Fragments arranged by size: 3334 1443 685 436 382 PvuI CG_AT'CG Cuts at: 104 104 Size: 6280 PvuII CAG'CTG Cuts at: 250 1447 1849 3703 4063 250 Size: 1197 402 1854 360 2467 Fragments arranged by size: 2467 1854 1197 402 360 RsrII CG'GwC_CG Cuts at: 4473 4473 Size: 6280 SacI G_AGCT'C Cuts at: 209 500 2194 3292 209 Size: 291 1694 1098 3197 Fragments arranged by size: 3197 1694 1098 291 SalI G'TCGA_C Cuts at: 3315 3315 Size: 6280 SapI GCTCTTCn'nnn_ Cuts at: 4301 4511 4301 Size: 210 6070 SbfI CC_TGCA'GG Cuts at: 3325 3325 Size: 6280 ScaI AGT'ACT Cuts at: 5797 5797 Size: 6280 SfcI C'TryA_G Cuts at: 33 315 1060 1442 1878 2139 3321 4006 5001 Size: 282 745 382 436 261 1182 685 995 Cuts at: 5001 5192 33 Size: 191 1121 Fragments arranged by size: 1182 1121 995 745 685 436 382 282 261 191 SfoI GGC'GCC Cuts at: 1773 3710 3958 1773 Size: 1937 248 4095 Fragments arranged by size: 4095 1937 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 6280 SmaI CCC'GGG Cuts at: 3300 3300 Size: 6280 SmlI C'TyrA_G Cuts at: 1354 1971 2422 3433 4348 4842 5104 1354 Size: 617 451 1011 915 494 262 2530 Fragments arranged by size: 2530 1011 915 617 494 451 262 SnaBI TAC'GTA Cuts at: 3270 3270 Size: 6280 SpeI A'CTAG_T Cuts at: 67 67 Size: 6280 SphI G_CATG'C Cuts at: 1927 3331 3831 1927 Size: 1404 500 4376 Fragments arranged by size: 4376 1404 500 StuI AGG'CCT Cuts at: 1206 2233 1206 Size: 1027 5253 StyI C'CwwG_G Cuts at: 125 230 374 1022 1201 1928 2540 2595 3412 Size: 105 144 648 179 727 612 55 817 Cuts at: 3412 3766 125 Size: 354 2639 Fragments arranged by size: 2639 817 727 648 612 354 179 144 105 55 SwaI ATTT'AAAT Cuts at: 572 572 Size: 6280 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1156 1426 1795 1849 2797 3861 3994 4453 4664 Size: 270 369 54 948 1064 133 459 211 Cuts at: 4664 1156 Size: 2772 Fragments arranged by size: 2772 1064 948 459 369 270 211 133 54 TatI w'GTAC_w Cuts at: 3182 5795 3182 Size: 2613 3667 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 164 196 761 793 1210 1242 2019 2051 2410 Size: 32 565 32 417 32 777 32 359 Cuts at: 2410 2442 2505 2537 5797 5829 164 Size: 32 63 32 3260 32 615 Fragments arranged by size: 3260 777 615 565 417 359 63 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 4075 4075 Size: 6280 XbaI T'CTAG_A Cuts at: 2196 3309 2196 Size: 1113 5167 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3773 3773 Size: 6280 XmaI C'CCGG_G Cuts at: 3298 3298 Size: 6280 XmnI GAAnn'nnTTC Cuts at: 434 434 Size: 6280 Enzymes that do cut: AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NruI NsiI NspI PciI PfoI PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SnaBI SpeI SphI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AfeI AhdI AleI AloI ApaI AscI AvrII BbvCI BcgI BglI BmgBI BmtI Bpu10I BsiWI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI NheI NotI PacI PasI PflMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI SrfI SspI XhoI ZraI