################################## # NO CUT ENZYMES ( 78 enzymes) # ################################## AatII AccII AclI AflII AgeI Aor51HI AscI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BfrI BlnI BsiEI BsiWI BsmI BspDI BsrBI BssHII BssSI BstBI BstEII BstPI BstUI ClaI CpoI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FauI FseI FspI HincII HindII HpaI KasI KspI MluI MvnI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RsrII SacI SacII SalI SbfI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tth111I ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 3574 GGTACC G^GTACC AccIII 2358 TCCGGA T^CCGGA AcyI 3529 GGCGTC G[GA]^CG[CT]C AflIII 47 ACATGT A^C[GA][CT]GT AseI 3010 ATTAAT AT^TAAT AsnI 3010 ATTAAT AT^TAAT Asp718I 3574 GGTACC G^GTACC BalI 387 TGGCCA TGG^CCA BciVI 396 GGATAC GTATCC(6/5) BclI 3765 TGATCA T^GATCA BglI 3373 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2792 AGATCT A^GATCT BsaAI 3049 TACGTG [CT]AC^GT[GA] BsaBI 627 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 3529 GGCGTC G[GA]^CG[CT]C BsaI 3410 GGTCTC GGTCTC(1/5) BsaWI 2358 TCCGGA [AT]^CCGG[AT] BseAI 2358 TCCGGA T^CCGGA BsmBI 3531 CGTCTC CGTCTC(1/5) BspEI 2358 TCCGGA T^CCGGA BspLU11I 47 ACATGT A^CATGT BsrDI 3361 GCAATG GCAATG(2/0) BstAPI 3342 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 2614 TTTAAA TTT^AAA EaeI 387 TGGCCA [CT]^GGCC[GA] EcoRI 1258 GAATTC G^AATTC EcoT22I 1982 ATGCAT ATGCA^T FbaI 3765 TGATCA T^GATCA HgaI 3530 GCGTC GACGC(5/10) Hin1I 3529 GGCGTC G[GA]^CG[CT]C KpnI 3574 GGTACC GGTAC^C MamI 627 GATTATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 3749 CAATTG C^AATTG MluNI 387 TGGCCA TGG^CCA MroI 2358 TCCGGA T^CCGGA MscI 387 TGGCCA TGG^CCA MunI 3749 CAATTG C^AATTG NsiI 1982 ATGCAT ATGCA^T PaeR7I 2213 CTCGAG C^TCGAG PflMI 4434 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 3010 ATTAAT AT^TAAT ScaI 1974 AGTACT AGT^ACT SexAI 4439 ACCTGGT A^CC[AT]GGT StuI 4175 AGGCCT AGG^CCT Van91I 4434 CCATTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2213 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccI 1751 GTATAC GT^[AC][GT]AC 3067 GTATAC AhdI 4049 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4341 GACATCTTGTC AocI 319 CCTGAGG CC^T[ACGT]AGG 4503 CCTGAGG AspEI 4049 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4341 GACATCTTGTC BanI 891 GGTGCC G^G[CT][GA]CC 3574 GGTACC BlpI 2680 GCTGAGC GC^T[ACGT]AGC 3895 GCTGAGC Bpu1102I 2680 GCTGAGC GC^T[ACGT]AGC 3895 GCTGAGC BspHI 998 TCATGA T^CATGA 2328 TCATGA BsrFI 3626 ACCGGC [GA]^CCGG[CT] 3656 ACCGGC Bst1107I 1751 GTATAC GTA^TAC 3067 GTATAC BstXI 3182 CCAGGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3602 CCACAAGCCTGG BstZ17I 1751 GTATAC GTA^TAC 3067 GTATAC Bsu36I 319 CCTGAGG CC^T[ACGT]AGG 4503 CCTGAGG CelII 2680 GCTGAGC GC^T[ACGT]AGC 3895 GCTGAGC Cfr10I 3626 ACCGGC [GA]^CCGG[CT] 3656 ACCGGC DsaI 1736 CCATGG C^C[GA][CT]GG 4484 CCATGG Eam1105I 4049 GACCAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4341 GACATCTTGTC EarI 4138 CTCTTC CTCTTC(1/4) 2752 GAAGAG Eco81I 319 CCTGAGG CC^T[ACGT]AGG 4503 CCTGAGG EcoNI 2023 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3693 CCTGGGCGAGG HindIII 296 AAGCTT A^AGCTT 1526 AAGCTT Ksp632I 4138 CTCTTC CTCTTC(1/4) 2752 GAAGAG MaeII 3050 ACGT A^CGT 4073 ACGT NcoI 1736 CCATGG C^CATGG 4484 CCATGG NdeI 1290 CATATG CA^TATG 1382 CATATG PshAI 1960 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2203 GACCCTAGTC PstI 2809 CTGCAG CTGCA^G 4400 CTGCAG RcaI 998 TCATGA T^CATGA 2328 TCATGA SapI 2752 GAAGAGC GCTCTTC(1/4) 4137 GCTCTTC SmaI 2727 CCCGGG CCC^GGG 3503 CCCGGG SspI 409 AATATT AAT^ATT 1809 AATATT XbaI 123 TCTAGA T^CTAGA 2412 TCTAGA XmaCI 2727 CCCGGG C^CCGGG 3503 CCCGGG XmaI 2727 CCCGGG C^CCGGG 3503 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI ApoI Asp700I AspHI AvaI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI