Restriction map for pF1KSDA0529
 (Linear) (Six-base) MAPSORT of: KIAA0529.seq  Check: 2320  from: 1  to: 2856



KIAA0529.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

Acc65I G'GTAC_C

Cuts at:      0      89    2856 
   Size:         89    2767
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     489    1739    1805    1814    1973    2342    2681 
   Size:        302     187    1250      66       9     159     369     339

Cuts at:   2681    2750    2856 
   Size:         69     106

  Fragments arranged by size: 

                1250     369     339     302     187     159     106      69
                  66       9
 
AflII C'TTAA_G

Cuts at:      0     235    2856 
   Size:        235    2621
 
AflIII A'CryG_T

Cuts at:      0     802    2856 
   Size:        802    2054
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2544    2856 
   Size:       2544     312
 
ApoI r'AATT_y

Cuts at:      0     891    1633    2500    2856 
   Size:        891     742     867     356

  Fragments arranged by size: 

                 891     867     742     356
 
AseI AT'TA_AT

Cuts at:      0    1829    2510    2856 
   Size:       1829     681     346

  Fragments arranged by size: 

                1829     681     346
 
AvaI C'yCGr_G

Cuts at:      0    1644    2856 
   Size:       1644    1212
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    2450    2483    2856 
   Size:       2450      33     373

  Fragments arranged by size: 

                2450     373      33
 
BamHI G'GATC_C

Cuts at:      0    1362    1798    2172    2856 
   Size:       1362     436     374     684

  Fragments arranged by size: 

                1362     684     436     374
 
BanI G'GyrC_C

Cuts at:      0      89    2856 
   Size:         89    2767
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     697    1727    2669    2856 
   Size:        697    1030     942     187

  Fragments arranged by size: 

                1030     942     697     187
 
BbvCI CC'TCA_GC

Cuts at:      0    2213    2856 
   Size:       2213     643
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     191    1998    2451    2856 
   Size:        191    1807     453     405

  Fragments arranged by size: 

                1807     453     405     191
 
BclI T'GATC_A

Cuts at:      0     947    2856 
   Size:        947    1909
 
BglI GCCn_nnn'nGGC

Cuts at:      0     779    1847    2856 
   Size:        779    1068    1009

  Fragments arranged by size: 

                1068    1009     779
 
BglII A'GATC_T

Cuts at:      0    1422    2149    2856 
   Size:       1422     727     707

  Fragments arranged by size: 

                1422     727     707
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     222    2856 
   Size:        222    2634
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2381    2856 
   Size:       2381     475
 
Bpu10I CC'TnA_GC

Cuts at:      0    2134    2213    2856 
   Size:       2134      79     643

  Fragments arranged by size: 

                2134     643      79
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1767    2401    2856 
   Size:       1767     634     455

  Fragments arranged by size: 

                1767     634     455
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     520    2856 
   Size:        520    2336
 
BsaAI yAC'GTr

Cuts at:      0     803    1605    2856 
   Size:        803     802    1251

  Fragments arranged by size: 

                1251     803     802
 
BsaBI GATnn'nnATC

Cuts at:      0     875    2171    2856 
   Size:        875    1296     685

  Fragments arranged by size: 

                1296     875     685
 
BsaWI w'CCGG_w

Cuts at:      0      71    2856 
   Size:         71    2785
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1982    2591    2856 
   Size:       1982     609     265

  Fragments arranged by size: 

                1982     609     265
 
BseYI C'CCAG_C

Cuts at:      0      32    2856 
   Size:         32    2824
 
BsiHKAI G_wGCw'C

Cuts at:      0    1604    2856 
   Size:       1604    1252
 
Bsp1286I G_dGCh'C

Cuts at:      0    1604    2856 
   Size:       1604    1252
 
BspEI T'CCGG_A

Cuts at:      0      71    2856 
   Size:         71    2785
 
BspHI T'CATG_A

Cuts at:      0     809    2856 
   Size:        809    2047
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1467    2512    2856 
   Size:       1467    1045     344

  Fragments arranged by size: 

                1467    1045     344
 
BsrBI CCG'CTC

Cuts at:      0      16    2856 
   Size:         16    2840
 
BsrDI GCAATG_nn'

Cuts at:      0    1312    2814    2856 
   Size:       1312    1502      42

  Fragments arranged by size: 

                1502    1312      42
 
BsrGI T'GTAC_A

Cuts at:      0    1429    2856 
   Size:       1429    1427
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1441    2856 
   Size:       1441    1415
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1663    2856 
   Size:       1663    1193
 
BstYI r'GATC_y

Cuts at:      0      21    1362    1422    1798    2149    2172    2325    2856 
   Size:         21    1341      60     376     351      23     153     531

  Fragments arranged by size: 

                1341     531     376     351     153      60      23      21
            
 
Bsu36I CC'TnA_GG

Cuts at:      0     906    2856 
   Size:        906    1950
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      30    2856 
   Size:         30    2826
 
BtsI GCAGTG_nn'

Cuts at:      0     375    1806    2651    2856 
   Size:        375    1431     845     205

  Fragments arranged by size: 

                1431     845     375     205
 
DraI TTT'AAA

Cuts at:      0    1193    2856 
   Size:       1193    1663
 
DraIII CAC_nnn'GTG

Cuts at:      0    1873    2856 
   Size:       1873     983
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2644    2856 
   Size:       2644     212
 
EaeI y'GGCC_r

Cuts at:      0     658    2856 
   Size:        658    2198
 
EarI CTCTTCn'nnn_

Cuts at:      0    2701    2856 
   Size:       2701     155
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19      26      34    2856 
   Size:         19       7       8    2822

  Fragments arranged by size: 

                2822      19       8       7
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     489    1739    1805    1814    1973    2342    2381 
   Size:        302     187    1250      66       9     159     369      39

Cuts at:   2381    2681    2750    2856 
   Size:        300      69     106

  Fragments arranged by size: 

                1250     369     302     300     187     159     106      69
                  66      39       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     667    2524    2856 
   Size:        667    1857     332

  Fragments arranged by size: 

                1857     667     332
 
EcoRI G'AATT_C

Cuts at:      0    2500    2856 
   Size:       2500     356
 
EcoRV GAT'ATC

Cuts at:      0    1039    2082    2856 
   Size:       1039    1043     774

  Fragments arranged by size: 

                1043    1039     774
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     686    1744    2856 
   Size:        686    1058    1112

  Fragments arranged by size: 

                1112    1058     686
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     153     185     214     246     309     341    1888    1920 
   Size:        153      32      29      32      63      32    1547      32

Cuts at:   1920    2134    2166    2371    2403    2856 
   Size:        214      32     205      32     453

  Fragments arranged by size: 

                1547     453     214     205     153      63      32      32
                  32      32      32      32      29
 
HincII GTy'rAC

Cuts at:      0    2020    2856 
   Size:       2020     836
 
KpnI G_GTAC'C

Cuts at:      0      93    2856 
   Size:         93    2763
 
MfeI C'AATT_G

Cuts at:      0     479    2856 
   Size:        479    2377
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     268    1944    2435    2477    2579    2856 
   Size:        268    1676     491      42     102     277

  Fragments arranged by size: 

                1676     491     277     268     102      42
 
MscI TGG'CCA

Cuts at:      0     660    2856 
   Size:        660    2196
 
MslI CAynn'nnrTG

Cuts at:      0     316    2234    2856 
   Size:        316    1918     622

  Fragments arranged by size: 

                1918     622     316
 
MspA1I CmG'CkG

Cuts at:      0      36     305    2770    2856 
   Size:         36     269    2465      86

  Fragments arranged by size: 

                2465     269      86      36
 
NdeI CA'TA_TG

Cuts at:      0    2688    2856 
   Size:       2688     168
 
NruI TCG'CGA

Cuts at:      0      49    2856 
   Size:         49    2807
 
PacI TTA_AT'TAA

Cuts at:      0    2510    2856 
   Size:       2510     346
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     536    2856 
   Size:        536    2320
 
PfoI T'CCnGG_A

Cuts at:      0     183    2856 
   Size:        183    2673
 
PmlI CAC'GTG

Cuts at:      0    1605    2856 
   Size:       1605    1251
 
PpuMI rG'GwC_Cy

Cuts at:      0     667    2524    2856 
   Size:        667    1857     332

  Fragments arranged by size: 

                1857     667     332
 
PsiI TTA'TAA

Cuts at:      0     725    1733    2856 
   Size:        725    1008    1123

  Fragments arranged by size: 

                1123    1008     725
 
PspXI vC'TCGA_Gb

Cuts at:      0    1644    2856 
   Size:       1644    1212
 
PstI C_TGCA'G

Cuts at:      0    1464    2856 
   Size:       1464    1392
 
PvuII CAG'CTG

Cuts at:      0     305    2770    2856 
   Size:        305    2465      86

  Fragments arranged by size: 

                2465     305      86
 
ScaI AGT'ACT

Cuts at:      0    2402    2856 
   Size:       2402     454
 
SexAI A'CCwGG_T

Cuts at:      0      85    2856 
   Size:         85    2771
 
SfcI C'TryA_G

Cuts at:      0    1460    2856 
   Size:       1460    1396
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     779    2856 
   Size:        779    2077
 
SmlI C'TyrA_G

Cuts at:      0     235    1644    1746    2416    2856 
   Size:        235    1409     102     670     440

  Fragments arranged by size: 

                1409     670     440     235     102
 
SspI AAT'ATT

Cuts at:      0    1511    1827    2856 
   Size:       1511     316    1029

  Fragments arranged by size: 

                1511    1029     316
 
StuI AGG'CCT

Cuts at:      0     773    2856 
   Size:        773    2083
 
StyI C'CwwG_G

Cuts at:      0     612     661    1337    2856 
   Size:        612      49     676    1519

  Fragments arranged by size: 

                1519     676     612      49
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      46    2762    2856 
   Size:         46    2716      94

  Fragments arranged by size: 

                2716      94      46
 
TatI w'GTAC_w

Cuts at:      0    1385    1429    1996    2400    2409    2691    2856 
   Size:       1385      44     567     404       9     282     165

  Fragments arranged by size: 

                1385     567     404     282     165      44       9
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     284    1828    2373    2856 
   Size:        284    1544     545     483

  Fragments arranged by size: 

                1544     545     483     284
 
XhoI C'TCGA_G

Cuts at:      0    1644    2856 
   Size:       1644    1212
 
XmnI GAAnn'nnTTC

Cuts at:      0    1203    2856 
   Size:       1203    1653
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII   AflIII     AlfI     ApoI     AseI     AvaI
     BaeI    BamHI     BanI     BbsI    BbvCI    BciVI     BclI     BglI
    BglII     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BstAPI    BstXI    BstYI   Bsu36I    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EarI     EciI  Eco57MI
 EcoO109I    EcoRI    EcoRV     FalI    Hin4I   HincII     KpnI     MfeI
     MmeI     MscI     MslI   MspA1I     NdeI     NruI     PacI    PflMI
     PfoI     PmlI    PpuMI     PsiI    PspXI     PstI    PvuII     ScaI
    SexAI     SfcI     SfiI     SmlI     SspI     StuI     StyI    TaqII
     TatI     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI     AgeI     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     AscI    AsiSI    AvrII    BanII
     BbeI     BcgI    BfrBI     BlpI Bme1580I    BmgBI     BmtI     BplI
    BsaHI    BsaXI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BsrFI
   BssHII    BssSI    BstBI   BstEII  BstZ17I     BtgI     ClaI    CspCI
     EagI  EcoICRI    EcoNI     FseI     FspI    FspAI    HaeII  HindIII
     HpaI     KasI     MluI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NsiI     NspI     PasI     PciI     PmeI     PpiI    PshAI
   PspOMI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SwaI     TstI  Tth111I     XbaI     XmaI     ZraI
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