Restriction map for pF1KSDA0478
 (Linear) (Six-base) MAPSORT of: KIAA0478.seq  Check: 6046  from: 1  to: 3717



KIAA0478.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2362    3513    3552    3591    3717 
   Size:       2362    1151      39      39     126

  Fragments arranged by size: 

                2362    1151     126      39      39
 
AccI GT'mk_AC

Cuts at:      0     297    1004    3717 
   Size:        297     707    2713

  Fragments arranged by size: 

                2713     707     297
 
Acc65I G'GTAC_C

Cuts at:      0      91    3065    3717 
   Size:         91    2974     652

  Fragments arranged by size: 

                2974     652      91
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     473     650    1256    1886    2159    2572    2869 
   Size:         87     386     177     606     630     273     413     297

Cuts at:   2869    2943    3000    3717 
   Size:         74      57     717

  Fragments arranged by size: 

                 717     630     606     413     386     297     273     177
                  87      74      57
 
AfeI AGC'GCT

Cuts at:      0    2269    3717 
   Size:       2269    1448
 
AflIII A'CryG_T

Cuts at:      0    2422    2944    3717 
   Size:       2422     522     773

  Fragments arranged by size: 

                2422     773     522
 
AleI CACnn'nnGTG

Cuts at:      0    3138    3717 
   Size:       3138     579
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2311    3717 
   Size:       2311    1406
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      49      81    2572    2604    3717 
   Size:         49      32    2491      32    1113

  Fragments arranged by size: 

                2491    1113      49      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30      39    1822    2191    2774    3149    3717 
   Size:         30       9    1783     369     583     375     568

  Fragments arranged by size: 

                1783     583     568     375     369      30       9
 
ApaI G_GGCC'C

Cuts at:      0     508    3717 
   Size:        508    3209
 
ApaLI G'TGCA_C

Cuts at:      0    2257    2914    3717 
   Size:       2257     657     803

  Fragments arranged by size: 

                2257     803     657
 
ApoI r'AATT_y

Cuts at:      0     564     723    1141    1215    2053    2443    3717 
   Size:        564     159     418      74     838     390    1274

  Fragments arranged by size: 

                1274     838     564     418     390     159      74
 
AvaI C'yCGr_G

Cuts at:      0     202    3717 
   Size:        202    3515
 
BanI G'GyrC_C

Cuts at:      0      91    1675    3065    3717 
   Size:         91    1584    1390     652

  Fragments arranged by size: 

                1584    1390     652      91
 
BanII G_rGCy'C

Cuts at:      0       8     111     202     508    2384    3248    3416    3545 
   Size:          8     103      91     306    1876     864     168     129

Cuts at:   3545    3653    3717 
   Size:        108      64

  Fragments arranged by size: 

                1876     864     306     168     129     108     103      91
                  64       8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     685     741    1249    1294    2108    3717 
   Size:        685      56     508      45     814    1609

  Fragments arranged by size: 

                1609     814     685     508      56      45
 
BbvCI CC'TCA_GC

Cuts at:      0    1256    3717 
   Size:       1256    2461
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     193     227    3717 
   Size:        193      34    3490

  Fragments arranged by size: 

                3490     193      34
 
BclI T'GATC_A

Cuts at:      0    2127    3566    3717 
   Size:       2127    1439     151

  Fragments arranged by size: 

                2127    1439     151
 
BfrBI ATG'CAT

Cuts at:      0     187    2680    3717 
   Size:        187    2493    1037

  Fragments arranged by size: 

                2493    1037     187
 
BglII A'GATC_T

Cuts at:      0    2954    3717 
   Size:       2954     763
 
BlpI GC'TnA_GC

Cuts at:      0    1888    3717 
   Size:       1888    1829
 
Bme1580I G_kGCm'C

Cuts at:      0     508    2261    2918    2967    3717 
   Size:        508    1753     657      49     750

  Fragments arranged by size: 

                1753     750     657     508      49
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    2703    3717 
   Size:       2703    1014
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2934    3717 
   Size:       2934     783
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     482    1261    1505    2377    2998    3121    3368    3717 
   Size:        482     779     244     872     621     123     247     349

  Fragments arranged by size: 

                 872     779     621     482     349     247     244     123
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     285    1256    3717 
   Size:        285     971    2461

  Fragments arranged by size: 

                2461     971     285
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     416    3717 
   Size:        416    3301
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1098    2311    3388    3574    3717 
   Size:       1098    1213    1077     186     143

  Fragments arranged by size: 

                1213    1098    1077     186     143
 
BsaAI yAC'GTr

Cuts at:      0    2733    2945    3717 
   Size:       2733     212     772

  Fragments arranged by size: 

                2733     772     212
 
BsaWI w'CCGG_w

Cuts at:      0    2894    3311    3717 
   Size:       2894     417     406

  Fragments arranged by size: 

                2894     417     406
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      49      79     253     283    3717 
   Size:         49      30     174      30    3434

  Fragments arranged by size: 

                3434     174      49      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     220    1048    1264    1816    1819    3592    3717 
   Size:        220     828     216     552       3    1773     125

  Fragments arranged by size: 

                1773     828     552     220     216     125       3
 
BseYI C'CCAG_C

Cuts at:      0     606    3302    3443    3584    3717 
   Size:        606    2696     141     141     133

  Fragments arranged by size: 

                2696     606     141     141     133
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1033    1962    2317    2331    2390    2803    2883    3162 
   Size:       1033     929     355      14      59     413      80     279

Cuts at:   3162    3717 
   Size:        555

  Fragments arranged by size: 

                1033     929     555     413     355     279      80      59
                  14
 
BsiHKAI G_wGCw'C

Cuts at:      0       8    1030    1811    2261    2489    2918    3182    3248 
   Size:          8    1022     781     450     228     429     264      66

Cuts at:   3248    3380    3416    3473    3490    3653    3717 
   Size:        132      36      57      17     163      64

  Fragments arranged by size: 

                1022     781     450     429     264     228     163     132
                  66      64      57      36      17       8
 
BsmI GAATG_Cn'

Cuts at:      0     600     871    1841    3291    3717 
   Size:        600     271     970    1450     426

  Fragments arranged by size: 

                1450     970     600     426     271
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1392    3717 
   Size:       1392    2325
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     111     202     508    1030    1811    2261    2384 
   Size:          8     103      91     306     522     781     450     123

Cuts at:   2384    2489    2918    2967    3182    3248    3380    3416    3473 
   Size:        105     429      49     215      66     132      36      57

Cuts at:   3473    3490    3545    3653    3717 
   Size:         17      55     108      64

  Fragments arranged by size: 

                 781     522     450     429     306     215     132     123
                 108     105     103      91      66      64      57      55
                  49      36      17       8
 
BspHI T'CATG_A

Cuts at:      0    1407    2609    3717 
   Size:       1407    1202    1108

  Fragments arranged by size: 

                1407    1202    1108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1652    2362    3261    3513    3552    3591    3717 
   Size:       1652     710     899     252      39      39     126

  Fragments arranged by size: 

                1652     899     710     252     126      39      39
 
BsrBI CCG'CTC

Cuts at:      0    2982    3717 
   Size:       2982     735
 
BsrDI GCAATG_nn'

Cuts at:      0    3352    3717 
   Size:       3352     365
 
BsrFI r'CCGG_y

Cuts at:      0      20    3717 
   Size:         20    3697
 
BsrGI T'GTAC_A

Cuts at:      0      41     233    3717 
   Size:         41     192    3484

  Fragments arranged by size: 

                3484     192      41
 
BssSI C'ACGA_G

Cuts at:      0    3652    3717 
   Size:       3652      65
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      30    1605    2603    2861    3205    3717 
   Size:         30    1575     998     258     344     512

  Fragments arranged by size: 

                1575     998     512     344     258      30
 
BstEII G'GTnAC_C

Cuts at:      0     768    2991    3717 
   Size:        768    2223     726

  Fragments arranged by size: 

                2223     768     726
 
BstYI r'GATC_y

Cuts at:      0    2841    2954    3026    3717 
   Size:       2841     113      72     691

  Fragments arranged by size: 

                2841     691     113      72
 
Bsu36I CC'TnA_GG

Cuts at:      0    1735    2457    3717 
   Size:       1735     722    1260

  Fragments arranged by size: 

                1735    1260     722
 
BtgI C'CryG_G

Cuts at:      0    1898    2364    3371    3545    3717 
   Size:       1898     466    1007     174     172

  Fragments arranged by size: 

                1898    1007     466     174     172
 
BtsI GCAGTG_nn'

Cuts at:      0    1703    1889    1950    2305    2319    2601    3717 
   Size:       1703     186      61     355      14     282    1116

  Fragments arranged by size: 

                1703    1116     355     282     186      61      14
 
ClaI AT'CG_AT

Cuts at:      0     182    3717 
   Size:        182    3535
 
DraI TTT'AAA

Cuts at:      0     785    3717 
   Size:        785    2932
 
DraIII CAC_nnn'GTG

Cuts at:      0      50    2991    3717 
   Size:         50    2941     726

  Fragments arranged by size: 

                2941     726      50
 
EaeI y'GGCC_r

Cuts at:      0    1916    3717 
   Size:       1916    1801
 
EarI CTCTTCn'nnn_

Cuts at:      0    1232    2194    2401    2510    2888    3253    3433    3717 
   Size:       1232     962     207     109     378     365     180     284

  Fragments arranged by size: 

                1232     962     378     365     284     207     180     109
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2619    3717 
   Size:       2619    1098
 
EcoICRI GAG'CTC

Cuts at:      0       6    3246    3414    3651    3717 
   Size:          6    3240     168     237      66

  Fragments arranged by size: 

                3240     237     168      66       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      87     473     482     650    1256    1261    1505    1886 
   Size:         87     386       9     168     606       5     244     381

Cuts at:   1886    2159    2377    2572    2869    2943    2998    3000    3121 
   Size:        273     218     195     297      74      55       2     121

Cuts at:   3121    3368    3717 
   Size:        247     349

  Fragments arranged by size: 

                 606     386     381     349     297     273     247     244
                 218     195     168     121      87      74      55       9
                   5       2
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2011    3717 
   Size:       2011    1706
 
EcoO109I rG'GnC_Cy

Cuts at:      0     504     505    1077    2738    3299    3717 
   Size:        504       1     572    1661     561     418

  Fragments arranged by size: 

                1661     572     561     504     418       1
 
EcoRI G'AATT_C

Cuts at:      0     564    1141    3717 
   Size:        564     577    2576

  Fragments arranged by size: 

                2576     577     564
 
EcoRV GAT'ATC

Cuts at:      0    1553    3717 
   Size:       1553    2164
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     282    2220    3264    3717 
   Size:        282    1938    1044     453

  Fragments arranged by size: 

                1938    1044     453     282
 
HaeII r_GCGC'y

Cuts at:      0    2271    3717 
   Size:       2271    1446
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     160     192    1389    1421    3717 
   Size:        160      32    1197      32    2296

  Fragments arranged by size: 

                2296    1197     160      32      32
 
HindIII A'AGCT_T

Cuts at:      0     115     632    1507    1868    2229    3717 
   Size:        115     517     875     361     361    1488

  Fragments arranged by size: 

                1488     875     517     361     361     115
 
KpnI G_GTAC'C

Cuts at:      0      95    3069    3717 
   Size:         95    2974     648

  Fragments arranged by size: 

                2974     648      95
 
MfeI C'AATT_G

Cuts at:      0    3049    3717 
   Size:       3049     668
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     203    1386    1791    2982    3717 
   Size:        203    1183     405    1191     735

  Fragments arranged by size: 

                1191    1183     735     405     203
 
MslI CAynn'nnrTG

Cuts at:      0    2492    2906    3138    3227    3312    3717 
   Size:       2492     414     232      89      85     405

  Fragments arranged by size: 

                2492     414     405     232      89      85
 
MspA1I CmG'CkG

Cuts at:      0      27      39     610     628     720    1101    2355    3020 
   Size:         27      12     571      18      92     381    1254     665

Cuts at:   3020    3717 
   Size:        697

  Fragments arranged by size: 

                1254     697     665     571     381      92      27      18
                  12
 
NaeI GCC'GGC

Cuts at:      0      22    3717 
   Size:         22    3695
 
NcoI C'CATG_G

Cuts at:      0    1898    2364    3717 
   Size:       1898     466    1353

  Fragments arranged by size: 

                1898    1353     466
 
NgoMIV G'CCGG_C

Cuts at:      0      20    3717 
   Size:         20    3697
 
NsiI A_TGCA'T

Cuts at:      0     189    2682    3717 
   Size:        189    2493    1035

  Fragments arranged by size: 

                2493    1035     189
 
NspI r_CATG'y

Cuts at:      0    1717    2426    2466    2684    3208    3717 
   Size:       1717     709      40     218     524     509

  Fragments arranged by size: 

                1717     709     524     509     218      40
 
PciI A'CATG_T

Cuts at:      0    2422    3717 
   Size:       2422    1295
 
PfoI T'CCnGG_A

Cuts at:      0    3345    3717 
   Size:       3345     372
 
PmlI CAC'GTG

Cuts at:      0    2733    2945    3717 
   Size:       2733     212     772

  Fragments arranged by size: 

                2733     772     212
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      49      81    3717 
   Size:         49      32    3636

  Fragments arranged by size: 

                3636      49      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1077    2738    3299    3717 
   Size:       1077    1661     561     418

  Fragments arranged by size: 

                1661    1077     561     418
 
PspOMI G'GGCC_C

Cuts at:      0     504    3717 
   Size:        504    3213
 
PstI C_TGCA'G

Cuts at:      0      35     966    1973    2770    3272    3717 
   Size:         35     931    1007     797     502     445

  Fragments arranged by size: 

                1007     931     797     502     445      35
 
PvuII CAG'CTG

Cuts at:      0      27      39     628     720    1101    2355    3020    3717 
   Size:         27      12     589      92     381    1254     665     697

  Fragments arranged by size: 

                1254     697     665     589     381      92      27      12
            
 
SacI G_AGCT'C

Cuts at:      0       8    3248    3416    3653    3717 
   Size:          8    3240     168     237      64

  Fragments arranged by size: 

                3240     237     168      64       8
 
SanDI GG'GwC_CC

Cuts at:      0    3299    3717 
   Size:       3299     418
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2194    3253    3717 
   Size:       2194    1059     464

  Fragments arranged by size: 

                2194    1059     464
 
ScaI AGT'ACT

Cuts at:      0    2689    3717 
   Size:       2689    1028
 
SfcI C'TryA_G

Cuts at:      0      15      31     298     623     962    1969    2766    3268 
   Size:         15      16     267     325     339    1007     797     502

Cuts at:   3268    3717 
   Size:        449

  Fragments arranged by size: 

                1007     797     502     449     339     325     267      16
                  15
 
SmlI C'TyrA_G

Cuts at:      0     431    3717 
   Size:        431    3286
 
SphI G_CATG'C

Cuts at:      0    2466    2684    3717 
   Size:       2466     218    1033

  Fragments arranged by size: 

                2466    1033     218
 
SspI AAT'ATT

Cuts at:      0     952    3717 
   Size:        952    2765
 
StuI AGG'CCT

Cuts at:      0    1967    2218    2626    3040    3717 
   Size:       1967     251     408     414     677

  Fragments arranged by size: 

                1967     677     414     408     251
 
StyI C'CwwG_G

Cuts at:      0    1133    1199    1898    2321    2364    3717 
   Size:       1133      66     699     423      43    1353

  Fragments arranged by size: 

                1353    1133     699     423      66      43
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1212    2635    3488    3717 
   Size:       1212    1423     853     229

  Fragments arranged by size: 

                1423    1212     853     229
 
TatI w'GTAC_w

Cuts at:      0      41     233    2687    3320    3717 
   Size:         41     192    2454     633     397

  Fragments arranged by size: 

                2454     633     397     192      41
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     253     285    3103    3135    3717 
   Size:        253      32    2818      32     582

  Fragments arranged by size: 

                2818     582     253      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1047    3717 
   Size:       1047    2670
 
XbaI T'CTAG_A

Cuts at:      0     732    1563    3717 
   Size:        732     831    2154

  Fragments arranged by size: 

                2154     831     732
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2371    3052    3717 
   Size:       2371     681     665

  Fragments arranged by size: 

                2371     681     665
 
XmnI GAAnn'nnTTC

Cuts at:      0     844    2203    2503    2956    3013    3717 
   Size:        844    1359     300     453      57     704

  Fragments arranged by size: 

                1359     844     704     453     300      57
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI   AflIII     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI     BanI    BanII
     BbsI    BbvCI     BcgI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaWI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI
   BstEII    BstYI   Bsu36I     BtgI     BtsI     ClaI     DraI   DraIII
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI    HaeII    Hin4I  HindIII     KpnI     MfeI     MmeI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI     PciI
     PfoI     PmlI     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI
    SanDI     SapI     ScaI     SfcI     SmlI     SphI     SspI     StuI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI    AflII     AgeI     AhdI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BciVI     BglI    BmgBI     BmtI
    BsaBI    BsaHI    BsiEI    BsiWI    BspEI   BssHII    BstBI    BstXI
  BstZ17I    BtgZI    CspCI     DrdI     EagI     FseI     FspI    FspAI
   HincII     HpaI     KasI     MluI     MscI     NarI     NdeI     NheI
     NotI     NruI     PacI     PasI    PflMI     PmeI    PshAI     PsiI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI
     SwaI     XhoI     XmaI     ZraI
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