Restriction map for pF1KSDA0470
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AatII       Acc65I      AccII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvrII       BbeI        BbrPI       BcgI        
BglI        BlnI        BsiWI       BspDI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BstBI       
BstEII      BstPI       BstUI       Bsu36I      Cfr10I      
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco52I      Eco81I      EcoO65I     EcoRV       FauI        
FseI        HpaI        KasI        KpnI        KspI        
MfeI        MluI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       Sse8387I    SwaI        Tth111I     XcmI        
XhoI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

AccIII        219  TCCGGA               T^CCGGA
AclI         1448  AACGTT               AA^CGTT
AcyI         1202  GACGCC               G[GA]^CG[CT]C
Aor51HI      3406  AGCGCT               AGC^GCT
Asp700I      2257  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII        3014  TGCGCA               TGC^GCA
BalI         4236  TGGCCA               TGG^CCA
BamHI        2352  GGATCC               G^GATCC
BanI          981  GGCACC               G^G[CT][GA]CC
BciVI        3088  GTATCC               GTATCC(6/5)
BlpI         3157  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     3157  GCTCAGC              GC^T[ACGT]AGC
BsaAI        2429  TACGTG               [CT]AC^GT[GA]
BsaHI        1202  GACGCC               G[GA]^CG[CT]C
BsaI          884  GAGACC               GGTCTC(1/5)
BseAI         219  TCCGGA               T^CCGGA
BsiEI        3771  CGGTCG               CG[GA][CT]^CG
BsmBI        1629  GAGACG               CGTCTC(1/5)
BsmI         4296  GAATGC               GAATGC(1/-1)
Bsp1407I     2895  TGTACA               T^GTACA
BspEI         219  TCCGGA               T^CCGGA
BspHI         777  TCATGA               T^CATGA
BsrGI        2895  TGTACA               T^GTACA
BssSI          56  CACGAG               CACGAG(-5/-1)
CelII        3157  GCTCAGC              GC^T[ACGT]AGC
DraII         311  GGGTCCC              [GA]G^G[ACGT]CC[CT]
DraIII       2114  CACAAAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3337  GACTCCAAGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         4236  TGGCCA               [CT]^GGCC[GA]
Eco47III     3406  AGCGCT               AGC^GCT
EcoO109I      311  GGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI         739  GAATTC               G^AATTC
EcoT22I      3679  ATGCAT               ATGCA^T
FspI         3014  TGCGCA               TGC^GCA
Hin1I        1202  GACGCC               G[GA]^CG[CT]C
HincII       2559  GTCAAC               GT[CT]^[GA]AC
HindII       2559  GTCAAC               GT[CT]^[GA]AC
HindIII      3035  AAGCTT               A^AGCTT
MluNI        4236  TGGCCA               TGG^CCA
MroI          219  TCCGGA               T^CCGGA
MscI         4236  TGGCCA               TGG^CCA
NcoI          491  CCATGG               C^CATGG
NsiI         3679  ATGCAT               ATGCA^T
PpuMI         311  GGGTCCC              [GA]G^G[AT]CC[CT]
Psp1406I     1448  AACGTT               AA^CGTT
RcaI          777  TCATGA               T^CATGA
SacI           91  GAGCTC               GAGCT^C
SapI         4255  GCTCTTC              GCTCTTC(1/4)
SmaI         4215  CCCGGG               CCC^GGG
SphI          828  GCATGC               GCATG^C
SspBI        2895  TGTACA               T^GTACA
SspI         3948  AATATT               AAT^ATT
StuI         1306  AGGCCT               AGG^CCT
XmaCI        4215  CCCGGG               C^CCGGG
XmaI         4215  CCCGGG               C^CCGGG
XmnI         2257  GAACTTATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflII         328  CTTAAG               C^TTAAG
             1197  CTTAAG              
AspHI          91  GAGCTC               G[AT]GC[AT]^C
             3020  GAGCAC              
AvaI         3536  CTCGGG               C^[CT]CG[GA]G
             4215  CCCGGG              
BcnI         4215  CCCGG                CC^[GC]GG
             4216  CCGGG               
BfrI          328  CTTAAG               C^TTAAG
             1197  CTTAAG              
BsaBI         274  GATACAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1285  GATGACAATC          
BsiHKAI        91  GAGCTC               G[AT]GC[AT]^C
             3020  GAGCAC              
BsoBI        3536  CTCGGG               C^[CT]CG[GA]G
             4215  CCCGGG              
BsrDI        3531  CATTGC               GCAATG(2/0)
             3418  GCAATG              
Bst1107I     1762  GTATAC               GTA^TAC
             4322  GTATAC              
BstXI         612  CCATGAAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2693  CCACAGATGTGG        
BstZ17I      1762  GTATAC               GTA^TAC
             4322  GTATAC              
DraI         2047  TTTAAA               TTT^AAA
             3955  TTTAAA              
DsaI          491  CCATGG               C^C[GA][CT]GG
              513  CCGTGG              
MamI          274  GATACAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1285  GATGACAATC          
NciI         4215  CCCGG                CC^[GC]GG
             4216  CCGGG               
NheI         1008  GCTAGC               G^CTAGC
             1609  GCTAGC              
NspI          828  GCATGC               [GA]CATG^[CT]
             2892  GCATGT              
PflMI         523  CCATTATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4228  CCAACACTTGG         
PstI          668  CTGCAG               CTGCA^G
             1364  CTGCAG              
ScaI          687  AGTACT               AGT^ACT
             1585  AGTACT              
SpeI         3276  ACTAGT               A^CTAGT
             3427  ACTAGT              
Van91I        523  CCATTATATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4228  CCAACACTTGG         
XbaI         2415  TCTAGA               T^CTAGA
             3220  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AlwNI       ApoI        AvaII       BanII       
BbsI        BbvCI       BbvI        BclI        BfaI        
BglII       BmyI        BpmI        BpuAI       BsaJI       
BsaWI       BseRI       BsgI        BsiYI       BslI        
BsmAI       BsmFI       Bsp1286I    BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       EarI        
EcoNI       EcoRII      EcoT14I     FbaI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PleI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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