Restriction map for pF1KB8804
##################################
# NO CUT ENZYMES (  158 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BciVI       BcnI        BfrI        
BglI        BmyI        BsaAI       BsaHI       BsaI        
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsoBI       Bsp1286I    BspDI       
BspEI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstZ17I     Bsu36I      
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        EagI        Eam1105I    EarI        
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FseI        
FspI        HaeII       HapII       HgaI        HhaI        
Hin1I       HinP1I      HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MfeI        
MluI        MroI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PshBI       Psp1406I    PstI        PvuI        RsrII       
SacI        SacII       SalI        SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspI        StuI        SwaI        TspRI       
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AciI          567  CCGC                 CCGC(-3/-1)
AlwI          464  GGATC                GGATC(4/5)
AlwNI         382  CAGTTTCTG            CAG[ACGT][ACGT][ACGT]^CTG
AvrII         573  CCTAGG               C^CTAGG
BalI          950  TGGCCA               TGG^CCA
BbsI          974  GAAGAC               GAAGAC(2/6)
BcgI          839  GCATGGACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          812  TGATCA               T^GATCA
BglII         611  AGATCT               A^GATCT
BlnI          573  CCTAGG               C^CTAGG
BlpI          501  GCTGAGC              GC^T[ACGT]AGC
BpmI          969  CTGGAG               CTGGAG(16/14)
Bpu1102I      501  GCTGAGC              GC^T[ACGT]AGC
BpuAI         974  GAAGAC               GAAGAC(2/6)
BsaBI          87  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI         994  GAGGAG               GAGGAG(10/8)
BsiYI         240  CCTGAATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          240  CCTGAATTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         430  GTCCC                GGGAC(10/14)
BspHI         680  TCATGA               T^CATGA
BssKI         968  CCTGG                ^CC[ACGT]GG
BstAPI        190  GCAATTTATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI         968  CCTGG                CC^[AT]GG
BstXI         179  CCATGGTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI         611  AGATCT               [GA]^GATC[CT]
Cac8I         169  GCAGGC               GC[ACGT]^[ACGT]GC
CelII         501  GCTGAGC              GC^T[ACGT]AGC
DsaI          179  CCATGG               C^C[GA][CT]GG
EaeI          950  TGGCCA               [CT]^GGCC[GA]
EcoRII        968  CCTGG                ^CC[AT]GG
FauI          566  CCCGC                CCCGC(4/6)
FbaI          812  TGATCA               T^GATCA
HaeIII        951  GGCC                 GG^CC
HincII         21  GTCAAC               GT[CT]^[GA]AC
HindII         21  GTCAAC               GT[CT]^[GA]AC
HphI          815  TCACC                GGTGA(8/7)
MaeII         938  ACGT                 A^CGT
MamI           87  GATCTGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          611  AGATCT               [GA]^GATC[CT]
MluNI         950  TGGCCA               TGG^CCA
MscI          950  TGGCCA               TGG^CCA
MvaI          968  CCTGG                CC^[AT]GG
NcoI          179  CCATGG               C^CATGG
PshAI          67  GACTACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI          680  TCATGA               T^CATGA
ScaI         1036  AGTACT               AGT^ACT
ScrFI         968  CCTGG                CC^[ACGT]GG
TaqI          708  TCGA                 T^CGA
TthHB8I       708  TCGA                 T^CGA
XhoII         611  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AcsI          112  GAATTT               [GA]^AATT[CT]
              243  GAATTT              
ApoI          112  GAATTT               [GA]^AATT[CT]
              243  GAATTT              
AvaII         965  GGTCC                G^G[AT]CC
             1018  GGTCC               
BsaJI         179  CCATGG               C^C[ACGT][ACGT]GG
              573  CCTAGG              
BsmAI         981  GAGAC                GTCTC(1/5)
              370  GTCTC               
BsmI          377  GAATGC               GAATGC(1/-1)
              551  GCATTC              
Bsp1407I      233  TGTACA               T^GTACA
              353  TGTACA              
BsrGI         233  TGTACA               T^GTACA
              353  TGTACA              
BsrI           18  CCAGT                ACTGG(1/-1)
             1034  CCAGT               
Cfr13I        965  GGTCC                G^G[ACGT]CC
             1018  GGTCC               
DdeI          502  CTGAG                C^T[ACGT]AG
             1013  CTGAG               
DraII         964  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1017  GGGTCCT             
EcoO109I      964  GGGTCCT              [GA]G^G[ACGT]CC[CT]
             1017  GGGTCCT             
EcoT14I       179  CCATGG               C^C[AT][AT]GG
              573  CCTAGG              
HinfI         597  GATTC                G^A[ACGT]TC
             1009  GATTC               
MseI          296  TTAA                 T^TAA
              698  TTAA                
MslI          180  CATGGTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              681  CATGAAGATG          
NlaIV         964  GGGTCC               GG[ACGT]^[ACGT]CC
             1017  GGGTCC              
PpuMI         964  GGGTCCT              [GA]G^G[AT]CC[CT]
             1017  GGGTCCT             
Sau96I        965  GGTCC                G^G[ACGT]CC
             1018  GGTCC               
SspBI         233  TGTACA               T^GTACA
              353  TGTACA              
StyI          179  CCATGG               C^C[AT][AT]GG
              573  CCTAGG              
TfiI          597  GATTC                G^A[AT]TC
             1009  GATTC               
Tru9I         296  TTAA                 T^TAA
              698  TTAA                
Tsp45I        751  GTCAC                ^GT[GC]AC
              859  GTCAC               


#########################################
# 3 OR MORE CUT ENZYMES (   25 enzymes) #
#########################################

AfaI        AluI        BbvI        BfaI        BstF5I      
DpnII       Fnu4HI      FokI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MspA1I      
MwoI        NdeII       NlaIII      PvuII       RsaI        
Sau3AI      SfaNI       SfcI        TseI        Tsp509I     
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