Restriction map for pF1KB8804
 (Linear) (Six-base) MAPSORT of: KIBB8804.seq  Check: 5259  from: 1  to: 1050



KIBB8804.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329    1050 
   Size:        329     721
 
AlwNI CAG_nnn'CTG

Cuts at:      0     387    1050 
   Size:        387     663
 
ApoI r'AATT_y

Cuts at:      0     112     243    1050 
   Size:        112     131     807

  Fragments arranged by size: 

                 807     131     112
 
AvrII C'CTAG_G

Cuts at:      0     573    1050 
   Size:        573     477
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     981    1050 
   Size:        981      69
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     828     862    1050 
   Size:        828      34     188

  Fragments arranged by size: 

                 828     188      34
 
BclI T'GATC_A

Cuts at:      0     812    1050 
   Size:        812     238
 
BglII A'GATC_T

Cuts at:      0     611    1050 
   Size:        611     439
 
BlpI GC'TnA_GC

Cuts at:      0     502    1050 
   Size:        502     548
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     990    1050 
   Size:        990      60
 
BsaBI GATnn'nnATC

Cuts at:      0      91    1050 
   Size:         91     959
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1009    1050 
   Size:       1009      41
 
BseYI C'CCAG_C

Cuts at:      0     433     512    1050 
   Size:        433      79     538

  Fragments arranged by size: 

                 538     433      79
 
BsmI GAATG_Cn'

Cuts at:      0     383     551    1050 
   Size:        383     168     499

  Fragments arranged by size: 

                 499     383     168
 
BspHI T'CATG_A

Cuts at:      0     680    1050 
   Size:        680     370
 
BsrGI T'GTAC_A

Cuts at:      0     233     353    1050 
   Size:        233     120     697

  Fragments arranged by size: 

                 697     233     120
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     196    1050 
   Size:        196     854
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     186    1050 
   Size:        186     864
 
BstYI r'GATC_y

Cuts at:      0     611    1050 
   Size:        611     439
 
BtgI C'CryG_G

Cuts at:      0     179    1050 
   Size:        179     871
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     831     855    1050 
   Size:        831      24     195

  Fragments arranged by size: 

                 831     195      24
 
EaeI y'GGCC_r

Cuts at:      0     950    1050 
   Size:        950     100
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     990    1050 
   Size:        329     661      60

  Fragments arranged by size: 

                 661     329      60
 
EcoO109I rG'GnC_Cy

Cuts at:      0     965    1018    1050 
   Size:        965      53      32

  Fragments arranged by size: 

                 965      53      32
 
HincII GTy'rAC

Cuts at:      0      23    1050 
   Size:         23    1027
 
MscI TGG'CCA

Cuts at:      0     952    1050 
   Size:        952      98
 
MslI CAynn'nnrTG

Cuts at:      0     184     685    1050 
   Size:        184     501     365

  Fragments arranged by size: 

                 501     365     184
 
MspA1I CmG'CkG

Cuts at:      0     516     821     887    1050 
   Size:        516     305      66     163

  Fragments arranged by size: 

                 516     305     163      66
 
NcoI C'CATG_G

Cuts at:      0     179    1050 
   Size:        179     871
 
PfoI T'CCnGG_A

Cuts at:      0     967    1050 
   Size:        967      83
 
PpuMI rG'GwC_Cy

Cuts at:      0     965    1018    1050 
   Size:        965      53      32

  Fragments arranged by size: 

                 965      53      32
 
PshAI GACnn'nnGTC

Cuts at:      0      71    1050 
   Size:         71     979
 
PsiI TTA'TAA

Cuts at:      0     767    1050 
   Size:        767     283
 
PvuII CAG'CTG

Cuts at:      0     516     821     887    1050 
   Size:        516     305      66     163

  Fragments arranged by size: 

                 516     305     163      66
 
ScaI AGT'ACT

Cuts at:      0    1038    1050 
   Size:       1038      12
 
SfcI C'TryA_G

Cuts at:      0      69     251    1022    1050 
   Size:         69     182     771      28

  Fragments arranged by size: 

                 771     182      69      28
 
StyI C'CwwG_G

Cuts at:      0     179     573    1050 
   Size:        179     394     477

  Fragments arranged by size: 

                 477     394     179
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     299    1050 
   Size:        299     751
 
TatI w'GTAC_w

Cuts at:      0     233     353    1036    1050 
   Size:        233     120     683      14

  Fragments arranged by size: 

                 683     233     120      14
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    AvrII     BbsI     BcgI     BclI    BglII
     BlpI     BpmI    BsaBI    BseRI    BseYI     BsmI    BspHI    BsrGI
   BstAPI    BstXI    BstYI     BtgI    BtgZI     EaeI  Eco57MI EcoO109I
   HincII     MscI     MslI   MspA1I     NcoI     PfoI    PpuMI    PshAI
     PsiI    PvuII     ScaI     SfcI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI    BciVI    BfrBI     BglI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI    BstBI   BstEII  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI
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