Restriction map for pF1KSDA0441
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbvCI       BfrI        BglII       
BlnI        BsaBI       BsaHI       BsaI        BseAI       
BsiWI       BsmBI       BsmFI       BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BssHII      BssSI       
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstXI       BstZ17I     Cfr10I      ClaI        CpoI        
DraI        DrdI        Eam1105I    Eco47III    EcoO65I     
EcoRI       EcoRV       EcoT22I     FseI        FspI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        MvnI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmeI        PpuMI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SapI        SbfI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        Sse8387I    StuI        SwaI        
Tth111I     Van91I      XbaI        XhoI        XmaCI       
XmaI        


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# ONE CUT ENZYMES (   41 enzymes) #
###################################

Alw44I       1903  GTGCAC               G^TGCAC
AlwNI        2005  CAGCACCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI          874  CCTGAGG              CC^T[ACGT]AGG
ApaLI        1903  GTGCAC               G^TGCAC
AvaI         1638  CTCGGG               C^[CT]CG[GA]G
BanI          430  GGTGCC               G^G[CT][GA]CC
BbsI          488  GAAGAC               GAAGAC(2/6)
BcgI            4  GCAGAAACATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         263  GTATCC               GTATCC(6/5)
BfaI         1706  CTAG                 C^TAG
BlpI         2002  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2002  GCTCAGC              GC^T[ACGT]AGC
BpuAI         488  GAAGAC               GAAGAC(2/6)
BsaWI        1215  ACCGGA               [AT]^CCGG[AT]
BsiEI        1042  CGGCCG               CG[GA][CT]^CG
BsoBI        1638  CTCGGG               C^[CT]CG[GA]G
BsrBI        1039  GAGCGG               CCGCTC(-3/-3)
BsrDI         509  CATTGC               GCAATG(2/0)
Bsu36I        874  CCTGAGG              CC^T[ACGT]AGG
CelII        2002  GCTCAGC              GC^T[ACGT]AGC
CfoI          861  GCGC                 GCG^C
DsaI          825  CCATGG               C^C[GA][CT]GG
EagI         1042  CGGCCG               C^GGCCG
EclXI        1042  CGGCCG               C^GGCCG
Eco52I       1042  CGGCCG               C^GGCCG
Eco81I        874  CCTGAGG              CC^T[ACGT]AGG
EcoNI         360  CCTGGTAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          881  CCCGC                CCCGC(4/6)
HaeII         860  AGCGCC               [GA]GCGC^[CT]
HgaI           49  GACGC                GACGC(5/10)
HhaI          861  GCGC                 GCG^C
HinP1I        861  GCGC                 G^CGC
MaeI         1706  CTAG                 C^TAG
NcoI          825  CCATGG               C^CATGG
NspI         1548  GCATGC               [GA]CATG^[CT]
PshAI        1121  GACAGAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII        2014  CAGCTG               CAG^CTG
SacI         1969  GAGCTC               GAGCT^C
ScaI         1987  AGTACT               AGT^ACT
SexAI         359  ACCTGGT              A^CC[AT]GGT
SphI         1548  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

ApaI          146  GGGCCC               GGGCC^C
             1882  GGGCCC              
BbrPI        1906  CACGTG               CAC^GTG
             2053  CACGTG              
BclI         1140  TGATCA               T^GATCA
             1910  TGATCA              
BcnI          144  CCGGG                CC^[GC]GG
             1699  CCCGG               
BglI           21  GCCTTCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              863  GCCCTGGAGGC         
BsaAI        1906  CACGTG               [CT]AC^GT[GA]
             2053  CACGTG              
BsmI         1219  GAATGC               GAATGC(1/-1)
             1442  GCATTC              
BspMI        1051  ACCTGC               ACCTGC(4/8)
             2009  ACCTGC              
DraIII       1056  CACCGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2050  CACCACGTG           
EcoT14I       825  CCATGG               C^C[AT][AT]GG
             1922  CCAAGG              
FbaI         1140  TGATCA               T^GATCA
             1910  TGATCA              
HphI         1288  GGTGA                GGTGA(8/7)
             2049  TCACC               
MaeII        1907  ACGT                 A^CGT
             2054  ACGT                
MspA1I        882  CCGCTG               C[AC]G^C[GT]G
             2014  CAGCTG              
NciI          144  CCGGG                CC^[GC]GG
             1699  CCCGG               
PleI         1417  GACTC                GAGTC(4/5)
              750  GAGTC               
PmaCI        1906  CACGTG               CAC^GTG
             2053  CACGTG              
PmlI         1906  CACGTG               CAC^GTG
             2053  CACGTG              
SfaNI        1026  GATGC                GCATC(5/9)
             1558  GATGC               
SspI         1154  AATATT               AAT^ATT
             1603  AATATT              
StyI          825  CCATGG               C^C[AT][AT]GG
             1922  CCAAGG              
TaqI          753  TCGA                 T^CGA
             1368  TCGA                
TthHB8I       753  TCGA                 T^CGA
             1368  TCGA                
XcmI          224  CCATTTATATGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1922  CCAAGGAAACACTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        Asp700I     AspHI       AvaII       BalI        
BanII       BbvI        BmyI        BpmI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       Bsp1286I    Bsp1407I    BsrGI       BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstYI       
Cac8I       Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
ItaI        Ksp632I     MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MseI        
MslI        MspI        MvaI        MwoI        NdeII       
NlaIII      NlaIV       PstI        RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        SspBI       TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
XhoII       XmnI        
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