Restriction map for pF1KSDA0422
##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AclI        AcsI        AflII       
AgeI        AhdI        Aor51HI     ApoI        AscI        
AseI        AsnI        Asp718I     AspEI       AvrII       
BbrPI       BfrI        BlnI        BsiWI       BsmBI       
BsmI        Bsp1407I    BsrGI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstZ17I     CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoRI       
EcoRV       EcoT22I     FseI        HindIII     HpaI        
KpnI        Ksp632I     MfeI        MunI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SapI        SbfI        
SfuI        SgrAI       SnaBI       SpeI        Sse8387I    
SspBI       SspI        SwaI        XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccIII        212  TCCGGA               T^CCGGA
Asp700I       384  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI         1126  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1188  TGCGCA               TGC^GCA
BamHI        1995  GGATCC               G^GATCC
BciVI         276  GGATAC               GTATCC(6/5)
BclI         1911  TGATCA               T^GATCA
BlpI         2578  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     2578  GCTCAGC              GC^T[ACGT]AGC
BsaBI        1266  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         212  TCCGGA               T^CCGGA
BsiEI        3210  CGACCG               CG[GA][CT]^CG
BspDI        1894  ATCGAT               AT^CGAT
BspEI         212  TCCGGA               T^CCGGA
BspHI        1202  TCATGA               T^CATGA
BspLU11I     2279  ACATGT               A^CATGT
BstEII       3222  GGTGACC              G^GT[ACGT]ACC
BstPI        3222  GGTGACC              G^GT[ACGT]ACC
CelII        2578  GCTCAGC              GC^T[ACGT]AGC
ClaI         1894  ATCGAT               AT^CGAT
EcoNI        3234  CCTGTACCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      3222  GGTGACC              G^GT[ACGT]ACC
FbaI         1911  TGATCA               T^GATCA
FspI         1188  TGCGCA               TGC^GCA
HincII       2592  GTCGAC               GT[CT]^[GA]AC
HindII       2592  GTCGAC               GT[CT]^[GA]AC
KspI          963  CCGCGG               CCGC^GG
MamI         1266  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI         1616  ACGCGT               A^CGCGT
MroI          212  TCCGGA               T^CCGGA
MseI          833  TTAA                 T^TAA
PflFI        1126  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         1202  TCATGA               T^CATGA
SacI         1216  GAGCTC               GAGCT^C
SacII         963  CCGCGG               CCGC^GG
SalI         2592  GTCGAC               G^TCGAC
ScaI         1979  AGTACT               AGT^ACT
SexAI        3239  ACCAGGT              A^CC[AT]GGT
Tru9I         833  TTAA                 T^TAA
Tsp509I      3079  AATT                 ^AATT
Tth111I      1126  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmnI          384  GAAGCAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AflIII       1616  ACGCGT               A^C[GA][CT]GT
             2279  ACATGT              
Alw44I       1182  GTGCAC               G^TGCAC
             1438  GTGCAC              
AlwNI        1192  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1564  CAGTACCTG           
ApaLI        1182  GTGCAC               G^TGCAC
             1438  GTGCAC              
BcgI          996  GCATGAGAATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1707  GCAGCGGACTCG        
BglII        1106  AGATCT               A^GATCT
             1271  AGATCT              
BsaAI         637  TACGTG               [CT]AC^GT[GA]
             1382  TACGTG              
BsaI          958  GAGACC               GGTCTC(1/5)
             2476  GAGACC              
BsaWI         212  TCCGGA               [AT]^CCGG[AT]
             2672  ACCGGA              
BsrFI         596  ACCGGC               [GA]^CCGG[CT]
             1937  GCCGGT              
Cfr10I        596  ACCGGC               [GA]^CCGG[CT]
             1937  GCCGGT              
PpuMI        2406  GGGTCCC              [GA]G^G[AT]CC[CT]
             3203  GGGTCCC             
SfiI         1525  GGCCGGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             1689  GGCCATGCTGGCC       
SmaI         1316  CCCGGG               CCC^GGG
             2726  CCCGGG              
SmlI         1624  CTCAAG               C^T[CT][GA]AG
             1969  CTTGAG              
SphI          767  GCATGC               GCATG^C
             2574  GCATGC              
StuI          947  AGGCCT               AGG^CCT
             1790  AGGCCT              
XcmI         3029  CCATGCGGCTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             3084  CCAGATGAAGATTGG     
XmaCI        1316  CCCGGG               C^CCGGG
             2726  CCCGGG              
XmaI         1316  CCCGGG               C^CCGGG
             2726  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  116 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AocI        ApaI        AspHI       
AvaI        AvaII       BalI        BanI        BanII       
BbeI        BbsI        BbvCI       BbvI        BcnI        
BfaI        BglI        BmyI        BpmI        BpuAI       
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BspMI       BsrBI       BsrDI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstXI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
Eco81I      EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MluNI       MnlI        MscI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NcoI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SfoI        StyI        TaqI        TfiI        
TseI        Tsp45I      TspRI       TthHB8I     Van91I      
XhoII       
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