Restriction map for pF1KSDA0383
##################################
# NO CUT ENZYMES (   74 enzymes) #
##################################

AatII       AccIII      AgeI        AhdI        Alw44I      
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BbeI        BbrPI       BbvCI       BglI        BglII       
BlnI        BsaAI       BsaBI       BseAI       BspDI       
BspEI       BssHII      BssSI       Bst1107I    BstBI       
BstZ17I     ClaI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoRV       FseI        FspI        
KasI        KspI        MamI        MluI        MroI        
NarI        NotI        NruI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       PvuI        SacI        SacII       
SbfI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SwaI        
Tth111I     XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I       1277  GGTACC               G^GTACC
AclI         1569  AACGTT               AA^CGTT
AcyI         3556  GGCGTC               G[GA]^CG[CT]C
AflII         783  CTTAAG               C^TTAAG
AflIII       3109  ACATGT               A^C[GA][CT]GT
AocI         3172  CCTGAGG              CC^T[ACGT]AGG
Asp718I      1277  GGTACC               G^GTACC
AspHI        4146  GAGCAC               G[AT]GC[AT]^C
AvaI         4477  CCCGAG               C^[CT]CG[GA]G
BamHI        1081  GGATCC               G^GATCC
BcgI         3683  GCAAGGAAGTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI          783  CTTAAG               C^TTAAG
BlpI         2146  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2146  GCTGAGC              GC^T[ACGT]AGC
BsaHI        3556  GGCGTC               G[GA]^CG[CT]C
BsaWI        2923  ACCGGA               [AT]^CCGG[AT]
BsiEI        1030  CGACCG               CG[GA][CT]^CG
BsiHKAI      4146  GAGCAC               G[AT]GC[AT]^C
BsiWI        3954  CGTACG               C^GTACG
BsoBI        4477  CCCGAG               C^[CT]CG[GA]G
BspHI         981  TCATGA               T^CATGA
BspLU11I     3109  ACATGT               A^CATGT
BsrBI        2149  GAGCGG               CCGCTC(-3/-3)
BstEII       1109  GGTCACC              G^GT[ACGT]ACC
BstPI        1109  GGTCACC              G^GT[ACGT]ACC
BstXI        4457  CCATGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       3172  CCTGAGG              CC^T[ACGT]AGG
CelII        2146  GCTGAGC              GC^T[ACGT]AGC
CpoI          504  CGGACCG              CG^G[AT]CCG
DraI         2929  TTTAAA               TTT^AAA
DraIII       3137  CACCAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         3550  GACTGTGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I       3172  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1650  CCTCTCTAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1109  GGTCACC              G^GT[ACGT]ACC
EcoRI        1378  GAATTC               G^AATTC
EcoT22I      1091  ATGCAT               ATGCA^T
Hin1I        3556  GGCGTC               G[GA]^CG[CT]C
HpaI         4590  GTTAAC               GTT^AAC
KpnI         1277  GGTACC               GGTAC^C
MfeI          341  CAATTG               C^AATTG
MunI          341  CAATTG               C^AATTG
NaeI         2276  GCCGGC               GCC^GGC
NgoMIV       2276  GCCGGC               G^CCGGC
NheI         2167  GCTAGC               G^CTAGC
NsiI         1091  ATGCAT               ATGCA^T
PshAI        3859  GACCAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I     1569  AACGTT               AA^CGTT
RcaI          981  TCATGA               T^CATGA
RsrII         504  CGGACCG              CG^G[AT]CCG
SalI         3559  GTCGAC               G^TCGAC
SphI         4322  GCATGC               GCATG^C


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI         3559  GTCGAC               GT^[AC][GT]AC
             4111  GTAGAC              
AccII         472  CGCG                 CG^CG
             2884  CGCG                
Asp700I      1089  GAATGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2434  GAAAATATTC          
BalI         2866  TGGCCA               TGG^CCA
             3945  TGGCCA              
BanII        2596  GAGCCC               G[GA]GC[CT]^C
             3586  GAGCCC              
BciVI        1645  GGATAC               GTATCC(6/5)
              558  GTATCC              
BcnI         2268  CCCGG                CC^[GC]GG
             5275  CCCGG               
BsaI         2563  GAGACC               GGTCTC(1/5)
             1787  GGTCTC              
Bsp1407I     2942  TGTACA               T^GTACA
             5264  TGTACA              
BsrFI        1274  GCCGGT               [GA]^CCGG[CT]
             2276  GCCGGC              
BsrGI        2942  TGTACA               T^GTACA
             5264  TGTACA              
BstUI         472  CGCG                 CG^CG
             2884  CGCG                
BstYI        1081  GGATCC               [GA]^GATC[CT]
             3264  AGATCC              
Cfr10I       1274  GCCGGT               [GA]^CCGG[CT]
             2276  GCCGGC              
MflI         1081  GGATCC               [GA]^GATC[CT]
             3264  AGATCC              
MluNI        2866  TGGCCA               TGG^CCA
             3945  TGGCCA              
MscI         2866  TGGCCA               TGG^CCA
             3945  TGGCCA              
MvnI          472  CGCG                 CG^CG
             2884  CGCG                
NciI         2268  CCCGG                CC^[GC]GG
             5275  CCCGG               
NdeI          886  CATATG               CA^TATG
             5219  CATATG              
PflMI         117  CCATGGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4413  CCAGCAGCTGG         
SexAI        1318  ACCTGGT              A^CC[AT]GGT
             3138  ACCAGGT             
SspBI        2942  TGTACA               T^GTACA
             5264  TGTACA              
Van91I        117  CCATGGGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             4413  CCAGCAGCTGG         
XbaI          283  TCTAGA               T^CTAGA
             1765  TCTAGA              
XhoII        1081  GGATCC               [GA]^GATC[CT]
             3264  AGATCC              
XmnI         1089  GAATGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2434  GAAAATATTC          


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        AvaII       BanI        BbsI        
BbvI        BclI        BfaI        BmyI        BpmI        
BpuAI       BsaJI       BseRI       BsgI        BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EaeI        EarI        EcoO109I    EcoRII      EcoT14I     
FauI        FbaI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HindIII     HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NcoI        NdeII       NlaIII      NlaIV       NspI        
PleI        PpuMI       PstI        PvuII       RsaI        
SapI        Sau3AI      Sau96I      ScaI        ScrFI       
SfaNI       SfcI        SmlI        SspI        StuI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
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