Restriction map for
pF1KSDA0378
##################################
# NO CUT ENZYMES ( 106 enzymes) #
##################################
AatII Acc65I AccI AclI AflIII
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BanI BanII
BbeI BbrPI BcgI BciVI BcnI
BglI BlnI BlpI Bpu1102I BsaAI
BsiEI BsiWI BsmBI Bsp1407I BspLU11I
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV FseI HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NciI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI StuI
SwaI Van91I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 2133 GGCGTC G[GA]^CG[CT]C
AfaI 2692 GTAC GT^AC
AflII 568 CTTAAG C^TTAAG
AgeI 2180 ACCGGT A^CCGGT
Aor51HI 1793 AGCGCT AGC^GCT
AspI 126 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 899 CCCGAG C^[CT]CG[GA]G
AviII 2357 TGCGCA TGC^GCA
BbvCI 1377 CCTCAGC CCTCAGC(-5/-2)
BfrI 568 CTTAAG C^TTAAG
BglII 444 AGATCT A^GATCT
BsaBI 2752 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2133 GGCGTC G[GA]^CG[CT]C
BsgI 1742 CTGCAC GTGCAG(16/14)
BsmI 2418 GAATGC GAATGC(1/-1)
BsoBI 899 CCCGAG C^[CT]CG[GA]G
BspDI 2795 ATCGAT AT^CGAT
BspHI 198 TCATGA T^CATGA
BspMI 420 GCAGGT ACCTGC(4/8)
BsrBI 749 GAGCGG CCGCTC(-3/-3)
BsrDI 2607 CATTGC GCAATG(2/0)
BstXI 956 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 2795 ATCGAT AT^CGAT
DsaI 290 CCATGG C^C[GA][CT]GG
Eco47III 1793 AGCGCT AGC^GCT
EcoNI 2196 CCTCAAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1946 ATGCAT ATGCA^T
FspI 2357 TGCGCA TGC^GCA
HaeII 1793 AGCGCT [GA]GCGC^[CT]
Hin1I 2133 GGCGTC G[GA]^CG[CT]C
HincII 666 GTTGAC GT[CT]^[GA]AC
HindII 666 GTTGAC GT[CT]^[GA]AC
HindIII 1366 AAGCTT A^AGCTT
MaeII 172 ACGT A^CGT
MamI 2752 GATGACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NcoI 290 CCATGG C^CATGG
NsiI 1946 ATGCAT ATGCA^T
NspI 1944 ACATGC [GA]CATG^[CT]
PflFI 126 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2180 ACCGGT A^CCGGT
PstI 469 CTGCAG CTGCA^G
RcaI 198 TCATGA T^CATGA
RsaI 2692 GTAC GT^AC
SexAI 398 ACCAGGT A^CC[AT]GGT
SpeI 2688 ACTAGT A^CTAGT
Tth111I 126 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1998 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 1389 AAATTC [GA]^AATT[CT]
1577 AAATTC
ApoI 1389 AAATTC [GA]^AATT[CT]
1577 AAATTC
AspHI 754 GAGCAC G[AT]GC[AT]^C
1417 GTGCTC
BalI 2294 TGGCCA TGG^CCA
2510 TGGCCA
BclI 195 TGATCA T^GATCA
1308 TGATCA
BsaI 707 GAGACC GGTCTC(1/5)
68 GGTCTC
BsiHKAI 754 GAGCAC G[AT]GC[AT]^C
1417 GTGCTC
BsrFI 1570 GCCGGT [GA]^CCGG[CT]
2180 ACCGGT
Cfr10I 1570 GCCGGT [GA]^CCGG[CT]
2180 ACCGGT
EcoT14I 64 CCAAGG C^C[AT][AT]GG
290 CCATGG
FauI 1966 GCGGG CCCGC(4/6)
2673 GCGGG
FbaI 195 TGATCA T^GATCA
1308 TGATCA
MluNI 2294 TGGCCA TGG^CCA
2510 TGGCCA
MscI 2294 TGGCCA TGG^CCA
2510 TGGCCA
NdeI 2269 CATATG CA^TATG
2862 CATATG
PpuMI 401 AGGTCCC [GA]G^G[AT]CC[CT]
1541 AGGACCT
SspI 307 AATATT AAT^ATT
2068 AATATT
StyI 64 CCAAGG C^C[AT][AT]GG
290 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AccII AccIII AciI AluI AlwI
AlwNI AvaII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BsaWI
BseAI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspEI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MroI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV PleI
PvuII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII