Restriction map for pF1KSDA0378
 (Linear) (Six-base) MAPSORT of: KIAA0378.seq  Check: 9659  from: 1  to: 2871



KIAA0378.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433    1081    1336    1447    1615    2871 
   Size:        433     648     255     111     168    1256

  Fragments arranged by size: 

                1256     648     433     255     168     111
 
AfeI AGC'GCT

Cuts at:      0    1795    2871 
   Size:       1795    1076
 
AflII C'TTAA_G

Cuts at:      0     568    2871 
   Size:        568    2303
 
AgeI A'CCGG_T

Cuts at:      0    2180    2871 
   Size:       2180     691
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1003    1741    2445    2487    2871 
   Size:       1003     738     704      42     384

  Fragments arranged by size: 

                1003     738     704     384      42
 
ApoI r'AATT_y

Cuts at:      0    1389    1577    2871 
   Size:       1389     188    1294

  Fragments arranged by size: 

                1389    1294     188
 
AvaI C'yCGr_G

Cuts at:      0     899    2871 
   Size:        899    1972
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     169     202    2871 
   Size:        169      33    2669

  Fragments arranged by size: 

                2669     169      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     850    1303    2375    2437    2871 
   Size:        850     453    1072      62     434

  Fragments arranged by size: 

                1072     850     453     434      62
 
BbvCI CC'TCA_GC

Cuts at:      0    1378    2871 
   Size:       1378    1493
 
BclI T'GATC_A

Cuts at:      0     195    1308    2871 
   Size:        195    1113    1563

  Fragments arranged by size: 

                1563    1113     195
 
BfrBI ATG'CAT

Cuts at:      0    1948    2871 
   Size:       1948     923
 
BglII A'GATC_T

Cuts at:      0     444    2871 
   Size:        444    2427
 
Bme1580I G_kGCm'C

Cuts at:      0     235    2871 
   Size:        235    2636
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2276    2871 
   Size:       2276     595
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     576     709     985    1111    2067    2209    2269    2446 
   Size:        576     133     276     126     956     142      60     177

Cuts at:   2446    2563    2575    2871 
   Size:        117      12     296

  Fragments arranged by size: 

                 956     576     296     276     177     142     133     126
                 117      60      12
 
Bpu10I CC'TnA_GC

Cuts at:      0     563     821    1378    2871 
   Size:        563     258     557    1493

  Fragments arranged by size: 

                1493     563     557     258
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     604     606     946    1210    2182    2287    2653    2871 
   Size:        604       2     340     264     972     105     366     218

  Fragments arranged by size: 

                 972     604     366     340     264     218     105       2
            
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      74     701    2871 
   Size:         74     627    2170

  Fragments arranged by size: 

                2170     627      74
 
BsaBI GATnn'nnATC

Cuts at:      0    2756    2871 
   Size:       2756     115
 
BsaHI Gr'CG_yC

Cuts at:      0    2134    2871 
   Size:       2134     737
 
BsaWI w'CCGG_w

Cuts at:      0     491    2180    2519    2838    2871 
   Size:        491    1689     339     319      33

  Fragments arranged by size: 

                1689     491     339     319      33
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     266     296    2871 
   Size:        266      30    2575

  Fragments arranged by size: 

                2575     266      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      62     478     712     761     793    2544    2560    2869 
   Size:         62     416     234      49      32    1751      16     309

Cuts at:   2869    2871 
   Size:          2

  Fragments arranged by size: 

                1751     416     309     234      62      49      32      16
                   2
 
BseYI C'CCAG_C

Cuts at:      0     455    2871 
   Size:        455    2416
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1727    2871 
   Size:       1727    1144
 
BsiHKAI G_wGCw'C

Cuts at:      0     758    1421    2871 
   Size:        758     663    1450

  Fragments arranged by size: 

                1450     758     663
 
BsmI GAATG_Cn'

Cuts at:      0    2424    2871 
   Size:       2424     447
 
Bsp1286I G_dGCh'C

Cuts at:      0     235     758    1421    2871 
   Size:        235     523     663    1450

  Fragments arranged by size: 

                1450     663     523     235
 
BspEI T'CCGG_A

Cuts at:      0     491    2519    2838    2871 
   Size:        491    2028     319      33

  Fragments arranged by size: 

                2028     491     319      33
 
BspHI T'CATG_A

Cuts at:      0     198    2871 
   Size:        198    2673
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     411    2871 
   Size:        411    2460
 
BsrBI CCG'CTC

Cuts at:      0     751    2871 
   Size:        751    2120
 
BsrDI GCAATG_nn'

Cuts at:      0    2606    2871 
   Size:       2606     265
 
BsrFI r'CCGG_y

Cuts at:      0    1570    2180    2871 
   Size:       1570     610     691

  Fragments arranged by size: 

                1570     691     610
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     166    2578    2608    2871 
   Size:        166    2412      30     263

  Fragments arranged by size: 

                2412     263     166      30
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     963    2871 
   Size:        963    1908
 
BstYI r'GATC_y

Cuts at:      0      49     444     526    1185    1199    2192    2549    2871 
   Size:         49     395      82     659      14     993     357     322

  Fragments arranged by size: 

                 993     659     395     357     322      82      49      14
            
 
BtgI C'CryG_G

Cuts at:      0     290    2871 
   Size:        290    2581
 
ClaI AT'CG_AT

Cuts at:      0    2796    2871 
   Size:       2796      75
 
EaeI y'GGCC_r

Cuts at:      0    1568    2294    2489    2510    2871 
   Size:       1568     726     195      21     361

  Fragments arranged by size: 

                1568     726     361     195      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     590    1244    1547    1832    2871 
   Size:        590     654     303     285    1039

  Fragments arranged by size: 

                1039     654     590     303     285
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1005    2871 
   Size:       1005    1866
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     433     576     709     985    1081    1111    1336    1447 
   Size:        433     143     133     276      96      30     225     111

Cuts at:   1447    1615    2067    2209    2269    2446    2563    2575    2871 
   Size:        168     452     142      60     177     117      12     296

  Fragments arranged by size: 

                 452     433     296     276     225     177     168     143
                 142     133     117     111      96      60      30      12
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    2200    2871 
   Size:       2200     671
 
EcoO109I rG'GnC_Cy

Cuts at:      0     402     678    1542    2831    2871 
   Size:        402     276     864    1289      40

  Fragments arranged by size: 

                1289     864     402     276      40
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1447    1678    2593    2871 
   Size:       1447     231     915     278

  Fragments arranged by size: 

                1447     915     278     231
 
FspI TGC'GCA

Cuts at:      0    2359    2871 
   Size:       2359     512
 
HaeII r_GCGC'y

Cuts at:      0    1797    2871 
   Size:       1797    1074
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     266     298     991    1023    1055    1107    1139    1831 
   Size:        266      32     693      32      32      52      32     692

Cuts at:   1831    1863    1903    1935    2871 
   Size:         32      40      32     936

  Fragments arranged by size: 

                 936     693     692     266      52      40      32      32
                  32      32      32      32
 
HincII GTy'rAC

Cuts at:      0     668    2871 
   Size:        668    2203
 
HindIII A'AGCT_T

Cuts at:      0    1366    2871 
   Size:       1366    1505
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1322    2300    2871 
   Size:       1322     978     571

  Fragments arranged by size: 

                1322     978     571
 
MscI TGG'CCA

Cuts at:      0    2296    2512    2871 
   Size:       2296     216     359

  Fragments arranged by size: 

                2296     359     216
 
MslI CAynn'nnrTG

Cuts at:      0     289     335    1141    1414    2871 
   Size:        289      46     806     273    1457

  Fragments arranged by size: 

                1457     806     289     273      46
 
MspA1I CmG'CkG

Cuts at:      0     459    1314    2541    2871 
   Size:        459     855    1227     330

  Fragments arranged by size: 

                1227     855     459     330
 
NcoI C'CATG_G

Cuts at:      0     290    2871 
   Size:        290    2581
 
NdeI CA'TA_TG

Cuts at:      0    2270    2863    2871 
   Size:       2270     593       8

  Fragments arranged by size: 

                2270     593       8
 
NsiI A_TGCA'T

Cuts at:      0    1950    2871 
   Size:       1950     921
 
NspI r_CATG'y

Cuts at:      0    1948    2871 
   Size:       1948     923
 
PfoI T'CCnGG_A

Cuts at:      0    2083    2552    2871 
   Size:       2083     469     319

  Fragments arranged by size: 

                2083     469     319
 
PpuMI rG'GwC_Cy

Cuts at:      0     402    1542    2871 
   Size:        402    1140    1329

  Fragments arranged by size: 

                1329    1140     402
 
PstI C_TGCA'G

Cuts at:      0     473    2871 
   Size:        473    2398
 
PvuII CAG'CTG

Cuts at:      0     459    1314    2541    2871 
   Size:        459     855    1227     330

  Fragments arranged by size: 

                1227     855     459     330
 
SexAI A'CCwGG_T

Cuts at:      0     398    2871 
   Size:        398    2473
 
SfcI C'TryA_G

Cuts at:      0     469     661    1537    2871 
   Size:        469     192     876    1334

  Fragments arranged by size: 

                1334     876     469     192
 
SmlI C'TyrA_G

Cuts at:      0     568     585     619     925    1189    2197    2302    2668 
   Size:        568      17      34     306     264    1008     105     366

Cuts at:   2668    2871 
   Size:        203

  Fragments arranged by size: 

                1008     568     366     306     264     203     105      34
                  17
 
SpeI A'CTAG_T

Cuts at:      0    2688    2871 
   Size:       2688     183
 
SspI AAT'ATT

Cuts at:      0     309    2070    2871 
   Size:        309    1761     801

  Fragments arranged by size: 

                1761     801     309
 
StyI C'CwwG_G

Cuts at:      0      64     290    2871 
   Size:         64     226    2581

  Fragments arranged by size: 

                2581     226      64
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     790    2699    2871 
   Size:        790    1909     172

  Fragments arranged by size: 

                1909     790     172
 
TatI w'GTAC_w

Cuts at:      0    2691    2871 
   Size:       2691     180
 
Tth111I GACn'n_nGTC

Cuts at:      0     129    2871 
   Size:        129    2742
 
XbaI T'CTAG_A

Cuts at:      0    1998    2871 
   Size:       1998     873
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     733    2442    2800    2871 
   Size:        733    1709     358      71

  Fragments arranged by size: 

                1709     733     358      71
 

 Enzymes that do cut:

     AcuI     AfeI    AflII     AgeI    AlwNI     ApoI     AvaI     BaeI
     BbsI    BbvCI     BclI    BfrBI    BglII Bme1580I     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI     ClaI
     EaeI     EarI     EciI  Eco57MI    EcoNI EcoO109I     FalI     FspI
    HaeII    Hin4I   HincII  HindIII     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NsiI     NspI     PfoI    PpuMI     PstI    PvuII
    SexAI     SfcI     SmlI     SpeI     SspI     StyI    TaqII     TatI
  Tth111I     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI   AflIII     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI     BanI    BanII     BbeI     BcgI    BciVI     BglI     BlpI
    BmgBI     BmrI     BmtI    BsaAI    BsiEI    BsiWI    BsmBI    BsrGI
   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoRI    EcoRV
     FseI    FspAI     HpaI     KasI     KpnI     MfeI     MluI     NaeI
     NarI   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI
    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SphI     SrfI     StuI     SwaI     TstI     XhoI     XmaI     XmnI
     ZraI
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