Restriction map for pF1KSDA0355
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflIII      AgeI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspI        AviII       AvrII       BamHI       
BbeI        BbrPI       BciVI       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiWI       BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BssHII      BssSI       Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     Bsu36I      
CelII       ClaI        CpoI        DraI        DrdI        
Eco47III    Eco81I      EcoNI       EcoO65I     EcoRV       
EcoT22I     FseI        FspI        HpaI        KasI        
KpnI        KspI        MamI        MfeI        MluI        
MroI        MunI        NarI        NdeI        NheI        
NotI        NruI        NsiI        NspV        PacI        
PflFI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacII       SalI        SapI        SbfI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        SphI        Sse8387I    SwaI        Tth111I     


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccII        2646  CGCG                 CG^CG
AcyI         1057  GGCGTC               G[GA]^CG[CT]C
AflII        1096  CTTAAG               C^TTAAG
AhdI         1422  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        1422  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BclI         1926  TGATCA               T^GATCA
BfrI         1096  CTTAAG               C^TTAAG
BglI         2603  GCCGGCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI        1057  GGCGTC               G[GA]^CG[CT]C
BsmBI        1059  CGTCTC               CGTCTC(1/5)
BsmI         2486  GAATGC               GAATGC(1/-1)
Bsp1407I     2363  TGTACA               T^GTACA
BsrDI         889  GCAATG               GCAATG(2/0)
BsrGI        2363  TGTACA               T^GTACA
BstUI        2646  CGCG                 CG^CG
EagI         1076  CGGCCG               C^GGCCG
Eam1105I     1422  GACCCTGGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        1076  CGGCCG               C^GGCCG
Eco52I       1076  CGGCCG               C^GGCCG
FbaI         1926  TGATCA               T^GATCA
HaeII          44  GGCGCT               [GA]GCGC^[CT]
Hin1I        1057  GGCGTC               G[GA]^CG[CT]C
HincII       1270  GTTGAC               GT[CT]^[GA]AC
HindII       1270  GTTGAC               GT[CT]^[GA]AC
MvnI         2646  CGCG                 CG^CG
NcoI         2030  CCATGG               C^CATGG
NspI          366  GCATGT               [GA]CATG^[CT]
PaeR7I       2736  CTCGAG               C^TCGAG
PpuMI        2433  GGGACCT              [GA]G^G[AT]CC[CT]
SacI         1012  GAGCTC               GAGCT^C
ScaI          280  AGTACT               AGT^ACT
SexAI        1190  ACCAGGT              A^CC[AT]GGT
SmaI         2618  CCCGGG               CCC^GGG
SspBI        2363  TGTACA               T^GTACA
SspI         1747  AATATT               AAT^ATT
StuI         3161  AGGCCT               AGG^CCT
XhoI         2736  CTCGAG               C^TCGAG
XmaCI        2618  CCCGGG               C^CCGGG
XmaI         2618  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

BcgI         1213  CGAGGTGCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             3072  CGACTGCAGTGC        
BglII         294  AGATCT               A^GATCT
             1529  AGATCT              
BseRI        2318  CTCCTC               GAGGAG(10/8)
             2875  CTCCTC              
BsiEI        1076  CGGCCG               CG[GA][CT]^CG
             2598  CGGTCG              
BspMI        1556  ACCTGC               ACCTGC(4/8)
             2308  GCAGGT              
DraII        2433  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2790  AGGGCCT             
DraIII        119  CACTGAGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1356  CACATGGTG           
EarI          298  CTCTTC               CTCTTC(1/4)
             1723  GAAGAG              
EcoO109I     2433  GGGACCT              [GA]G^G[ACGT]CC[CT]
             2790  AGGGCCT             
EcoRI         472  GAATTC               G^AATTC
             1824  GAATTC              
EcoT14I      2030  CCATGG               C^C[AT][AT]GG
             3149  CCAAGG              
Ksp632I       298  CTCTTC               CTCTTC(1/4)
             1723  GAAGAG              
NaeI          898  GCCGGC               GCC^GGC
             2603  GCCGGC              
NgoMIV        898  GCCGGC               G^CCGGC
             2603  GCCGGC              
PflMI         567  CCAGCCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2915  CCAAAACGTGG         
StyI         2030  CCATGG               C^C[AT][AT]GG
             3149  CCAAGG              
TaqI          830  TCGA                 T^CGA
             2737  TCGA                
TthHB8I       830  TCGA                 T^CGA
             2737  TCGA                
Van91I        567  CCAGCCGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2915  CCAAAACGTGG         
XbaI          712  TCTAGA               T^CTAGA
             1330  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AspHI       AvaI        
AvaII       BalI        BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BmyI        
BpmI        BpuAI       BsaJI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrFI       BsrI        BssKI       BstAPI      
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EaeI        EcoRII      FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HindIII     HinfI       HpaII       HphI        
ItaI        MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XcmI        XhoII       XmnI        
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