Restriction map for pF1KSDA0353
 (Linear) (Six-base) MAPSORT of: KIAA0353.seq  Check: 8773  from: 1  to: 4695



KIAA0353.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     885    1432    2452    4695 
   Size:        885     547    1020    2243

  Fragments arranged by size: 

                2243    1020     885     547
 
AccI GT'mk_AC

Cuts at:      0    4083    4607    4695 
   Size:       4083     524      88

  Fragments arranged by size: 

                4083     524      88
 
Acc65I G'GTAC_C

Cuts at:      0    3769    4695 
   Size:       3769     926
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1960    3023    3177    3212    3416    4695 
   Size:       1960    1063     154      35     204    1279

  Fragments arranged by size: 

                1960    1279    1063     204     154      35
 
AflIII A'CryG_T

Cuts at:      0      74    4695 
   Size:         74    4621
 
AgeI A'CCGG_T

Cuts at:      0    1819    4695 
   Size:       1819    2876
 
AhdI GACnn_n'nnGTC

Cuts at:      0    4614    4695 
   Size:       4614      81
 
AleI CACnn'nnGTG

Cuts at:      0    2008    4695 
   Size:       2008    2687
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1269    1301    4695 
   Size:       1269      32    3394

  Fragments arranged by size: 

                3394    1269      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     210     291    2844    3139    3348    3613    4695 
   Size:        210      81    2553     295     209     265    1082

  Fragments arranged by size: 

                2553    1082     295     265     210     209      81
 
ApaI G_GGCC'C

Cuts at:      0      28    3292    3715    4364    4695 
   Size:         28    3264     423     649     331

  Fragments arranged by size: 

                3264     649     423     331      28
 
ApaLI G'TGCA_C

Cuts at:      0     547    4027    4051    4695 
   Size:        547    3480      24     644

  Fragments arranged by size: 

                3480     644     547      24
 
ApoI r'AATT_y

Cuts at:      0    1006    2743    2794    4695 
   Size:       1006    1737      51    1901

  Fragments arranged by size: 

                1901    1737    1006      51
 
AvaI C'yCGr_G

Cuts at:      0      28     388     724    1200    2515    2881    3209    3267 
   Size:         28     360     336     476    1315     366     328      58

Cuts at:   3267    4077    4695 
   Size:        810     618

  Fragments arranged by size: 

                1315     810     618     476     366     360     336     328
                  58      28
 
AvrII C'CTAG_G

Cuts at:      0    3567    4695 
   Size:       3567    1128
 
BanI G'GyrC_C

Cuts at:      0    1139    1728    1861    3769    4695 
   Size:       1139     589     133    1908     926

  Fragments arranged by size: 

                1908    1139     926     589     133
 
BanII G_rGCy'C

Cuts at:      0      28      44      53    1201    2255    2834    3292    3490 
   Size:         28      16       9    1148    1054     579     458     198

Cuts at:   3490    3604    3715    4042    4364    4443    4695 
   Size:        114     111     327     322      79     252

  Fragments arranged by size: 

                1148    1054     579     458     327     322     252     198
                 114     111      79      28      16       9
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     907    3620    4695 
   Size:        907    2713    1075

  Fragments arranged by size: 

                2713    1075     907
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     322     356    1430    1464    1767    1801    2599    2633 
   Size:        322      34    1074      34     303      34     798      34

Cuts at:   2633    3584    3618    4695 
   Size:        951      34    1077

  Fragments arranged by size: 

                1077    1074     951     798     322     303      34      34
                  34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     782    1758    1945    4695 
   Size:        782     976     187    2750

  Fragments arranged by size: 

                2750     976     782     187
 
BfrBI ATG'CAT

Cuts at:      0     492    4695 
   Size:        492    4203
 
BglI GCCn_nnn'nGGC

Cuts at:      0     187     391    3326    3948    4695 
   Size:        187     204    2935     622     747

  Fragments arranged by size: 

                2935     747     622     204     187
 
BlpI GC'TnA_GC

Cuts at:      0     220     980    3055    4041    4695 
   Size:        220     760    2075     986     654

  Fragments arranged by size: 

                2075     986     760     654     220
 
Bme1580I G_kGCm'C

Cuts at:      0      28     551    1142    1162    1864    2525    3292    3715 
   Size:         28     523     591      20     702     661     767     423

Cuts at:   3715    4031    4055    4364    4695 
   Size:        316      24     309     331

  Fragments arranged by size: 

                 767     702     661     591     523     423     331     316
                 309      28      24      20
 
BmgBI CAC'GTC

Cuts at:      0     453    4695 
   Size:        453    4242
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    3286    3330    4006    4695 
   Size:       3286      44     676     689

  Fragments arranged by size: 

                3286     689     676      44
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1710    4695 
   Size:       1710    2985
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     115     136     646     772     847     937    1943 
   Size:         28      87      21     510     126      75      90    1006

Cuts at:   1943    2486    2713    2887    3067    3370    3423    3778    4488 
   Size:        543     227     174     180     303      53     355     710

Cuts at:   4488    4579    4695 
   Size:         91     116

  Fragments arranged by size: 

                1006     710     543     510     355     303     227     180
                 174     126     116      91      90      87      75      53
                  28      21
 
Bpu10I CC'TnA_GC

Cuts at:      0    1682    3115    4307    4342    4695 
   Size:       1682    1433    1192      35     353

  Fragments arranged by size: 

                1682    1433    1192     353      35
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1304    4695 
   Size:       1304    3391
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    4529    4695 
   Size:       4529     166
 
BsaAI yAC'GTr

Cuts at:      0      75    2010    2442    4695 
   Size:         75    1935     432    2253

  Fragments arranged by size: 

                2253    1935     432      75
 
BsaBI GATnn'nnATC

Cuts at:      0     970    2560    3032    4695 
   Size:        970    1590     472    1663

  Fragments arranged by size: 

                1663    1590     970     472
 
BsaHI Gr'CG_yC

Cuts at:      0     329    1792    4695 
   Size:        329    1463    2903

  Fragments arranged by size: 

                2903    1463     329
 
BsaWI w'CCGG_w

Cuts at:      0    1819    3493    4695 
   Size:       1819    1674    1202

  Fragments arranged by size: 

                1819    1674    1202
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1269    1299    1941    1971    2887    2917    3869    3899 
   Size:       1269      30     642      30     916      30     952      30

Cuts at:   3899    4695 
   Size:        796

  Fragments arranged by size: 

                1269     952     916     796     642      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     133     136     199     316     643     793     814    1612 
   Size:        133       3      63     117     327     150      21     798

Cuts at:   1612    2263    2299    2326    2371    2581    2811    2911    3064 
   Size:        651      36      27      45     210     230     100     153

Cuts at:   3064    4639    4681    4684    4687    4695 
   Size:       1575      42       3       3       8

  Fragments arranged by size: 

                1575     798     651     327     230     210     153     150
                 133     117     100      63      45      42      36      27
                  21       8       3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     224     336     369     399    1226    2679    3132    3525 
   Size:        224     112      33      30     827    1453     453     393

Cuts at:   3525    3618    3962    4525    4695 
   Size:         93     344     563     170

  Fragments arranged by size: 

                1453     827     563     453     393     344     224     170
                 112      93      33      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     616    2629    2663    4172    4609    4695 
   Size:        616    2013      34    1509     437      86

  Fragments arranged by size: 

                2013    1509     616     437      86      34
 
BsiEI CG_ry'CG

Cuts at:      0     135     321    3036    3494    4695 
   Size:        135     186    2715     458    1201

  Fragments arranged by size: 

                2715    1201     458     186     135
 
BsiHKAI G_wGCw'C

Cuts at:      0      44      53     551     986    4024    4031    4055    4111 
   Size:         44       9     498     435    3038       7      24      56

Cuts at:   4111    4443    4695 
   Size:        332     252

  Fragments arranged by size: 

                3038     498     435     332     252      56      44      24
                   9       7
 
BsmI GAATG_Cn'

Cuts at:      0    3185    4695 
   Size:       3185    1510
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     583     670    3018    4466    4695 
   Size:        583      87    2348    1448     229

  Fragments arranged by size: 

                2348    1448     583     229      87
 
Bsp1286I G_dGCh'C

Cuts at:      0      28      44      53     551     986    1142    1162    1201 
   Size:         28      16       9     498     435     156      20      39

Cuts at:   1201    1864    2255    2525    2834    3292    3490    3604    3715 
   Size:        663     391     270     309     458     198     114     111

Cuts at:   3715    4024    4031    4042    4055    4111    4364    4443    4695 
   Size:        309       7      11      13      56     253      79     252

  Fragments arranged by size: 

                 663     498     458     435     391     309     309     270
                 253     252     198     156     114     111      79      56
                  39      28      20      16      13      11       9       7
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     885    1432    2452    4695 
   Size:        885     547    1020    2243

  Fragments arranged by size: 

                2243    1020     885     547
 
BsrBI CCG'CTC

Cuts at:      0     252     804    3166    4695 
   Size:        252     552    2362    1529

  Fragments arranged by size: 

                2362    1529     552     252
 
BsrDI GCAATG_nn'

Cuts at:      0    4247    4349    4695 
   Size:       4247     102     346

  Fragments arranged by size: 

                4247     346     102
 
BsrFI r'CCGG_y

Cuts at:      0     134     511     904    1819    3503    4695 
   Size:        134     377     393     915    1684    1192

  Fragments arranged by size: 

                1684    1192     915     393     377     134
 
BsrGI T'GTAC_A

Cuts at:      0    3218    3885    4695 
   Size:       3218     667     810

  Fragments arranged by size: 

                3218     810     667
 
BssHII G'CGCG_C

Cuts at:      0     190     306     374     376     378     465     467     469 
   Size:        190     116      68       2       2      87       2       2

Cuts at:    469     471     689     741     743    4695 
   Size:          2     218      52       2    3952

  Fragments arranged by size: 

                3952     218     190     116      87      68      52       2
                   2       2       2       2       2
 
BssSI C'ACGA_G

Cuts at:      0    1512    4695 
   Size:       1512    3183
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1444    4695 
   Size:       1444    3251
 
BstEII G'GTnAC_C

Cuts at:      0    3466    4695 
   Size:       3466    1229
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     237    2339    2974    3078    3596    4501    4695 
   Size:        237    2102     635     104     518     905     194

  Fragments arranged by size: 

                2102     905     635     518     237     194     104
 
BstYI r'GATC_y

Cuts at:      0    1147    2555    4195    4695 
   Size:       1147    1408    1640     500

  Fragments arranged by size: 

                1640    1408    1147     500
 
BtgI C'CryG_G

Cuts at:      0     315     857    1143    2948    3978    4257    4458    4695 
   Size:        315     542     286    1805    1030     279     201     237

  Fragments arranged by size: 

                1805    1030     542     315     286     279     237     201
            
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    2393    4695 
   Size:       2393    2302
 
BtsI GCAGTG_nn'

Cuts at:      0     230     562    2651    3434    4695 
   Size:        230     332    2089     783    1261

  Fragments arranged by size: 

                2089    1261     783     332     230
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    2748    2783    3312    3347    4695 
   Size:       2748      35     529      35    1348

  Fragments arranged by size: 

                2748    1348     529      35      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1999    4695 
   Size:       1999    2696
 
EaeI y'GGCC_r

Cuts at:      0     132     318     572     645     854    1217    3299    4695 
   Size:        132     186     254      73     209     363    2082    1396

  Fragments arranged by size: 

                2082    1396     363     254     209     186     132      73
            
 
EagI C'GGCC_G

Cuts at:      0     132     318    4695 
   Size:        132     186    4377

  Fragments arranged by size: 

                4377     186     132
 
EarI CTCTTCn'nnn_

Cuts at:      0     668    1179    1556    1702    1755    3752    4695 
   Size:        668     511     377     146      53    1997     943

  Fragments arranged by size: 

                1997     943     668     511     377     146      53
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     256     682    2895    4695 
   Size:        256     426    2213    1800

  Fragments arranged by size: 

                2213    1800     426     256
 
EcoICRI GAG'CTC

Cuts at:      0      42      51    4441    4695 
   Size:         42       9    4390     254

  Fragments arranged by size: 

                4390     254      42       9
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     115     136     646     772     847     937    1943 
   Size:         28      87      21     510     126      75      90    1006

Cuts at:   1943    1960    2486    2713    2887    3023    3067    3177    3212 
   Size:         17     526     227     174     136      44     110      35

Cuts at:   3212    3370    3416    3423    3778    4488    4579    4695 
   Size:        158      46       7     355     710      91     116

  Fragments arranged by size: 

                1006     710     526     510     355     227     174     158
                 136     126     116     110      91      90      87      75
                  46      44      35      28      21      17       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     118     541     892    3475    3809    4310    4695 
   Size:        118     423     351    2583     334     501     385

  Fragments arranged by size: 

                2583     501     423     385     351     334     118
 
EcoO109I rG'GnC_Cy

Cuts at:      0      25     150     885    1368    2335    2700    3171    3254 
   Size:         25     125     735     483     967     365     471      83

Cuts at:   3254    3288    3430    3529    3571    3711    3712    3802    4219 
   Size:         34     142      99      42     140       1      90     417

Cuts at:   4219    4360    4695 
   Size:        141     335

  Fragments arranged by size: 

                 967     735     483     471     417     365     335     142
                 141     140     125      99      90      83      42      34
                  25       1
 
EcoRI G'AATT_C

Cuts at:      0    1006    2794    4695 
   Size:       1006    1788    1901

  Fragments arranged by size: 

                1901    1788    1006
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    4128    4695 
   Size:       4128     567
 
FspI TGC'GCA

Cuts at:      0     344    4695 
   Size:        344    4351
 
HaeII r_GCGC'y

Cuts at:      0     291     370     397     423     637     709     724    2686 
   Size:        291      79      27      26     214      72      15    1962

Cuts at:   2686    3139    4695 
   Size:        453    1556

  Fragments arranged by size: 

                1962    1556     453     291     214      79      72      27
                  26      15
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    2171    2203    2210    2242    2524    2556    2557    2589 
   Size:       2171      32       7      32     282      32       1      32

Cuts at:   2589    2887    2919    3403    3435    3545    3577    4600    4632 
   Size:        298      32     484      32     110      32    1023      32

Cuts at:   4632    4695 
   Size:         63

  Fragments arranged by size: 

                2171    1023     484     298     282     110      63      32
                  32      32      32      32      32      32      32       7
                   1
 
HincII GTy'rAC

Cuts at:      0    2232    3009    4695 
   Size:       2232     777    1686

  Fragments arranged by size: 

                2232    1686     777
 
KpnI G_GTAC'C

Cuts at:      0    3773    4695 
   Size:       3773     922
 
MfeI C'AATT_G

Cuts at:      0    1397    4695 
   Size:       1397    3298
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     710    1312    4695 
   Size:        710     602    3383

  Fragments arranged by size: 

                3383     710     602
 
MscI TGG'CCA

Cuts at:      0     856    4695 
   Size:        856    3839
 
MslI CAynn'nnrTG

Cuts at:      0    2008    2806    4695 
   Size:       2008     798    1889

  Fragments arranged by size: 

                2008    1889     798
 
MspA1I CmG'CkG

Cuts at:      0     176     210     317     600     738    2270    2844    2865 
   Size:        176      34     107     283     138    1532     574      21

Cuts at:   2865    4297    4695 
   Size:       1432     398

  Fragments arranged by size: 

                1532    1432     574     398     283     176     138     107
                  34      21
 
NaeI GCC'GGC

Cuts at:      0     136    4695 
   Size:        136    4559
 
NcoI C'CATG_G

Cuts at:      0     857    3978    4695 
   Size:        857    3121     717

  Fragments arranged by size: 

                3121     857     717
 
NdeI CA'TA_TG

Cuts at:      0    3423    4695 
   Size:       3423    1272
 
NgoMIV G'CCGG_C

Cuts at:      0     134    4695 
   Size:        134    4561
 
NotI GC'GGCC_GC

Cuts at:      0     318    4695 
   Size:        318    4377
 
NsiI A_TGCA'T

Cuts at:      0     494    4695 
   Size:        494    4201
 
NspI r_CATG'y

Cuts at:      0     999    3520    4695 
   Size:        999    2521    1175

  Fragments arranged by size: 

                2521    1175     999
 
PasI CC'CwG_GG

Cuts at:      0    2339    3285    4695 
   Size:       2339     946    1410

  Fragments arranged by size: 

                2339    1410     946
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1294    2717    3139    3613    3984    4602    4695 
   Size:       1294    1423     422     474     371     618      93

  Fragments arranged by size: 

                1423    1294     618     474     422     371      93
 
PfoI T'CCnGG_A

Cuts at:      0      44     113    4465    4695 
   Size:         44      69    4352     230

  Fragments arranged by size: 

                4352     230      69      44
 
PmlI CAC'GTG

Cuts at:      0    2442    4695 
   Size:       2442    2253
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1269    1301    1555    1587    1834    1866    4695 
   Size:       1269      32     254      32     247      32    2829

  Fragments arranged by size: 

                2829    1269     254     247      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     885    1368    2700    3430    3529    4219    4695 
   Size:        885     483    1332     730      99     690     476

  Fragments arranged by size: 

                1332     885     730     690     483     476      99
 
PshAI GACnn'nnGTC

Cuts at:      0    3473    3781    4266    4695 
   Size:       3473     308     485     429

  Fragments arranged by size: 

                3473     485     429     308
 
PspOMI G'GGCC_C

Cuts at:      0      24    3288    3711    4360    4695 
   Size:         24    3264     423     649     335

  Fragments arranged by size: 

                3264     649     423     335      24
 
PspXI vC'TCGA_Gb

Cuts at:      0     388    4695 
   Size:        388    4307
 
PstI C_TGCA'G

Cuts at:      0      20     287     362     413     896    3441    3636    3676 
   Size:         20     267      75      51     483    2545     195      40

Cuts at:   3676    3729    4695 
   Size:         53     966

  Fragments arranged by size: 

                2545     966     483     267     195      75      53      51
                  40      20
 
PvuI CG_AT'CG

Cuts at:      0    3036    4695 
   Size:       3036    1659
 
PvuII CAG'CTG

Cuts at:      0     210     600     738    2844    2865    4695 
   Size:        210     390     138    2106      21    1830

  Fragments arranged by size: 

                2106    1830     390     210     138      21
 
SacI G_AGCT'C

Cuts at:      0      44      53    4443    4695 
   Size:         44       9    4390     252

  Fragments arranged by size: 

                4390     252      44       9
 
SacII CC_GC'GG

Cuts at:      0     318    4695 
   Size:        318    4377
 
SanDI GG'GwC_CC

Cuts at:      0    3529    4695 
   Size:       3529    1166
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1755    4695 
   Size:       1755    2940
 
SbfI CC_TGCA'GG

Cuts at:      0     896    4695 
   Size:        896    3799
 
SfcI C'TryA_G

Cuts at:      0      16     283     358     409     892    1045    3437    3632 
   Size:         16     267      75      51     483     153    2392     195

Cuts at:   3632    3672    3725    4223    4695 
   Size:         40      53     498     472

  Fragments arranged by size: 

                2392     498     483     472     267     195     153      75
                  53      51      40      16
 
SmaI CCC'GGG

Cuts at:      0    2517    3211    4695 
   Size:       2517     694    1484

  Fragments arranged by size: 

                2517    1484     694
 
SmlI C'TyrA_G

Cuts at:      0     388    1319    4695 
   Size:        388     931    3376

  Fragments arranged by size: 

                3376     931     388
 
StuI AGG'CCT

Cuts at:      0     428    4519    4695 
   Size:        428    4091     176

  Fragments arranged by size: 

                4091     428     176
 
StyI C'CwwG_G

Cuts at:      0     851     857    2669    2825    3567    3978    4695 
   Size:        851       6    1812     156     742     411     717

  Fragments arranged by size: 

                1812     851     742     717     411     156       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     328    3856    3976    4695 
   Size:        328    3528     120     719

  Fragments arranged by size: 

                3528     719     328     120
 
TatI w'GTAC_w

Cuts at:      0    3218    3885    4695 
   Size:       3218     667     810

  Fragments arranged by size: 

                3218     810     667
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1941    1973    3513    3545    3723    3755    4695 
   Size:       1941      32    1540      32     178      32     940

  Fragments arranged by size: 

                1941    1540     940     178      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    4217    4695 
   Size:       4217     478
 
XbaI T'CTAG_A

Cuts at:      0    1740    4656    4695 
   Size:       1740    2916      39

  Fragments arranged by size: 

                2916    1740      39
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2974    3441    4695 
   Size:       2974     467    1254

  Fragments arranged by size: 

                2974    1254     467
 
XhoI C'TCGA_G

Cuts at:      0     388    4695 
   Size:        388    4307
 
XmaI C'CCGG_G

Cuts at:      0    2515    3209    4695 
   Size:       2515     694    1486

  Fragments arranged by size: 

                2515    1486     694
 
XmnI GAAnn'nnTTC

Cuts at:      0    1330    1570    1945    2876    4695 
   Size:       1330     240     375     931    1819

  Fragments arranged by size: 

                1819    1330     931     375     240
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI   AflIII     AgeI     AhdI     AleI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII     BanI
    BanII     BbsI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI   BstEII    BstXI    BstYI     BtgI
    BtgZI     BtsI    CspCI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KpnI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NcoI     NdeI   NgoMIV     NotI     NsiI     NspI     PasI
    PflMI     PfoI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI
     PstI     PvuI    PvuII     SacI    SacII    SanDI     SapI     SbfI
     SfcI     SmaI     SmlI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII     AlfI     AscI     AseI    AsiSI
     BaeI    BamHI     BbeI    BbvCI     BclI    BglII     BmtI    BsiWI
    BspEI    BspHI    BstBI  BstZ17I   Bsu36I     ClaI     DraI   DraIII
    EcoRV     FseI    FspAI  HindIII     HpaI     KasI     MluI     NarI
     NheI     NruI     PacI     PciI     PmeI     PsiI     PsrI    RsrII
     SalI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     ZraI
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