Restriction map for pF1KSDA0352
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AccII       AccIII      AclI        AflII       AgeI        
AhdI        AocI        Aor51HI     AscI        AseI        
AsnI        AspEI       AspI        AviII       BbeI        
BbrPI       BciVI       BfrI        BglII       BsaAI       
BsaBI       BsaWI       BseAI       BsiEI       BsiWI       
BsmBI       BsmI        BspDI       BspEI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstUI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FauI        FseI        FspI        
HincII      HindII      HindIII     HinfI       HpaI        
KasI        KspI        MamI        MfeI        MluI        
MroI        MunI        MvnI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
ScaI        SexAI       SfiI        SfoI        SfuI        
SnaBI       SpeI        Sse8387I    SwaI        TfiI        
Tth111I     


###################################
# ONE CUT ENZYMES (   51 enzymes) #
###################################

AatII          91  GACGTC               GACGT^C
Acc65I        427  GGTACC               G^GTACC
AcsI         1855  GAATTC               [GA]^AATT[CT]
ApaI         1970  GGGCCC               GGGCC^C
ApoI         1855  GAATTC               [GA]^AATT[CT]
Asp700I       168  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       427  GGTACC               G^GTACC
AvrII         687  CCTAGG               C^CTAGG
BalI         1379  TGGCCA               TGG^CCA
BamHI        1874  GGATCC               G^GATCC
BanI          427  GGTACC               G^G[CT][GA]CC
BbvCI         928  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1080  GCAGCATGCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI          287  TGATCA               T^GATCA
BglI          127  GCCCACAAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          687  CCTAGG               C^CTAGG
BlpI          316  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      316  GCTGAGC              GC^T[ACGT]AGC
Bsp1407I     2093  TGTACA               T^GTACA
BspHI        2024  TCATGA               T^CATGA
BsrDI         752  GCAATG               GCAATG(2/0)
BsrGI        2093  TGTACA               T^GTACA
BssSI         720  CACGAG               CACGAG(-5/-1)
CelII         316  GCTGAGC              GC^T[ACGT]AGC
EaeI         1379  TGGCCA               [CT]^GGCC[GA]
EcoRI        1855  GAATTC               G^AATTC
FbaI          287  TGATCA               T^GATCA
HaeII        1782  GGCGCT               [GA]GCGC^[CT]
KpnI          427  GGTACC               GGTAC^C
MluNI        1379  TGGCCA               TGG^CCA
MscI         1379  TGGCCA               TGG^CCA
NaeI           68  GCCGGC               GCC^GGC
NgoMIV         68  GCCGGC               G^CCGGC
PaeR7I        886  CTCGAG               C^TCGAG
PflMI        1590  CCAAAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI        1456  GACCAGCGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI         2024  TCATGA               T^CATGA
SapI         1774  GAAGAGC              GCTCTTC(1/4)
SgrAI        1867  CACCGGCG             C[GA]^CCGG[CT]G
SmaI         1157  CCCGGG               CCC^GGG
SmlI          886  CTCGAG               C^T[CT][GA]AG
SphI         1083  GCATGC               GCATG^C
SspBI        2093  TGTACA               T^GTACA
SspI         1266  AATATT               AAT^ATT
Tsp509I      1856  AATT                 ^AATT
Van91I       1590  CCAAAGTCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          200  CCAGGACCTATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI          886  CTCGAG               C^TCGAG
XmaCI        1157  CCCGGG               C^CCGGG
XmaI         1157  CCCGGG               C^CCGGG
XmnI          168  GAACCTCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          259  GTCTAC               GT^[AC][GT]AC
              799  GTAGAC              
AcyI           91  GACGTC               G[GA]^CG[CT]C
             1603  GACGCC              
AflIII       1208  ACATGT               A^C[GA][CT]GT
             2034  ACATGT              
Alw44I       1585  GTGCAC               G^TGCAC
             2044  GTGCAC              
ApaLI        1585  GTGCAC               G^TGCAC
             2044  GTGCAC              
BsaHI          91  GACGTC               G[GA]^CG[CT]C
             1603  GACGCC              
BspLU11I     1208  ACATGT               A^CATGT
             2034  ACATGT              
BsrBI         114  CCGCTC               CCGCTC(-3/-3)
             1932  CCGCTC              
BsrFI          68  GCCGGC               [GA]^CCGG[CT]
             1868  ACCGGC              
BstXI          98  CCATTGTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              459  CCATCCCCTTGG        
BstYI         852  AGATCC               [GA]^GATC[CT]
             1874  GGATCC              
CfoI         1358  GCGC                 GCG^C
             1783  GCGC                
Cfr10I         68  GCCGGC               [GA]^CCGG[CT]
             1868  ACCGGC              
EcoNI          45  CCTGCTGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              339  CCTCCTCCAGG         
HhaI         1358  GCGC                 GCG^C
             1783  GCGC                
Hin1I          91  GACGTC               G[GA]^CG[CT]C
             1603  GACGCC              
HinP1I       1358  GCGC                 G^CGC
             1783  GCGC                
MflI          852  AGATCC               [GA]^GATC[CT]
             1874  GGATCC              
MseI         1469  TTAA                 T^TAA
             2018  TTAA                
PvuII         152  CAGCTG               CAG^CTG
             1240  CAGCTG              
SacI          723  GAGCTC               GAGCT^C
              877  GAGCTC              
StuI          347  AGGCCT               AGG^CCT
             1439  AGGCCT              
Tru9I        1469  TTAA                 T^TAA
             2018  TTAA                
XbaI         1053  TCTAGA               T^CTAGA
             1566  TCTAGA              
XhoII         852  AGATCC               [GA]^GATC[CT]
             1874  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   81 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaI        AvaII       BanII       BbsI        
BbvI        BcnI        BfaI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       Cac8I       Cfr13I      
DdeI        DpnII       DraII       EarI        EcoO109I    
EcoRII      EcoT14I     Fnu4HI      FokI        HaeIII      
HapII       HgaI        HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       NspI        PpuMI       PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
StyI        TaqI        TseI        Tsp45I      TspRI       
TthHB8I     
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