Restriction map for pF1KSDA0352
 (Linear) (Six-base) MAPSORT of: KIAA0352.seq  Check: 3226  from: 1  to: 2136



KIAA0352.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AatII G_ACGT'C

Cuts at:      0      95    2136 
   Size:         95    2041
 
AccI GT'mk_AC

Cuts at:      0     260     800    2136 
   Size:        260     540    1336

  Fragments arranged by size: 

                1336     540     260
 
Acc65I G'GTAC_C

Cuts at:      0     427    2136 
   Size:        427    1709
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     251     747    2136 
   Size:         70     181     496    1389

  Fragments arranged by size: 

                1389     496     181      70
 
AflIII A'CryG_T

Cuts at:      0    1208    2034    2136 
   Size:       1208     826     102

  Fragments arranged by size: 

                1208     826     102
 
AleI CACnn'nnGTG

Cuts at:      0     100    2136 
   Size:        100    2036
 
AlwNI CAG_nnn'CTG

Cuts at:      0     454    1451    1651    2136 
   Size:        454     997     200     485

  Fragments arranged by size: 

                 997     485     454     200
 
ApaI G_GGCC'C

Cuts at:      0    1974    2136 
   Size:       1974     162
 
ApaLI G'TGCA_C

Cuts at:      0    1585    2044    2136 
   Size:       1585     459      92

  Fragments arranged by size: 

                1585     459      92
 
ApoI r'AATT_y

Cuts at:      0    1855    2136 
   Size:       1855     281
 
AvaI C'yCGr_G

Cuts at:      0     886    1088    1157    1965    2136 
   Size:        886     202      69     808     171

  Fragments arranged by size: 

                 886     808     202     171      69
 
AvrII C'CTAG_G

Cuts at:      0     687    2136 
   Size:        687    1449
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     411     444    2136 
   Size:        411      33    1692

  Fragments arranged by size: 

                1692     411      33
 
BamHI G'GATC_C

Cuts at:      0    1874    2136 
   Size:       1874     262
 
BanI G'GyrC_C

Cuts at:      0     427    2136 
   Size:        427    1709
 
BanII G_rGCy'C

Cuts at:      0     727     881    1061    1974    2136 
   Size:        727     154     180     913     162

  Fragments arranged by size: 

                 913     727     180     162     154
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     635     926    1512    1607    2136 
   Size:        635     291     586      95     529

  Fragments arranged by size: 

                 635     586     529     291      95
 
BbvCI CC'TCA_GC

Cuts at:      0     929    2136 
   Size:        929    1207
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1069    1103    2136 
   Size:       1069      34    1033

  Fragments arranged by size: 

                1069    1033      34
 
BclI T'GATC_A

Cuts at:      0     287    2136 
   Size:        287    1849
 
BglI GCCn_nnn'nGGC

Cuts at:      0     133    2136 
   Size:        133    2003
 
BlpI GC'TnA_GC

Cuts at:      0     317    2136 
   Size:        317    1819
 
Bme1580I G_kGCm'C

Cuts at:      0    1589    1974    2003    2048    2136 
   Size:       1589     385      29      45      88

  Fragments arranged by size: 

                1589     385      88      45      29
 
BmgBI CAC'GTC

Cuts at:      0     656    1668    2136 
   Size:        656    1012     468

  Fragments arranged by size: 

                1012     656     468
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     194     518     821    1227    2136 
   Size:        194     324     303     406     909

  Fragments arranged by size: 

                 909     406     324     303     194
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     438    2136 
   Size:        438    1698
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     394     554     865    1720    2136 
   Size:        328      66     160     311     855     416

  Fragments arranged by size: 

                 855     416     328     311     160      66
 
Bpu10I CC'TnA_GC

Cuts at:      0     929    2136 
   Size:        929    1207
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      73    1126    1327    2136 
   Size:         73    1053     201     809

  Fragments arranged by size: 

                1053     809     201      73
 
BsaHI Gr'CG_yC

Cuts at:      0      92    1604    2136 
   Size:         92    1512     532

  Fragments arranged by size: 

                1512     532      92
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     630     660    1138    1168    2136 
   Size:        630      30     478      30     968

  Fragments arranged by size: 

                 968     630     478      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     331     964    1774    2136 
   Size:        331     633     810     362

  Fragments arranged by size: 

                 810     633     362     331
 
BseYI C'CCAG_C

Cuts at:      0     677    1317    1728    2136 
   Size:        677     640     411     408

  Fragments arranged by size: 

                 677     640     411     408
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     170    1647    1971    2136 
   Size:        170    1477     324     165

  Fragments arranged by size: 

                1477     324     170     165
 
BsiHKAI G_wGCw'C

Cuts at:      0     382     727     845     881    1589    2048    2136 
   Size:        382     345     118      36     708     459      88

  Fragments arranged by size: 

                 708     459     382     345     118      88      36
 
Bsp1286I G_dGCh'C

Cuts at:      0     382     727     845     881    1061    1589    1974    2003 
   Size:        382     345     118      36     180     528     385      29

Cuts at:   2003    2048    2136 
   Size:         45      88

  Fragments arranged by size: 

                 528     385     382     345     180     118      88      45
                  36      29
 
BspHI T'CATG_A

Cuts at:      0    2024    2136 
   Size:       2024     112
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      53     462    1112    2136 
   Size:         53     409     650    1024

  Fragments arranged by size: 

                1024     650     409      53
 
BsrBI CCG'CTC

Cuts at:      0     116    1934    2136 
   Size:        116    1818     202

  Fragments arranged by size: 

                1818     202     116
 
BsrDI GCAATG_nn'

Cuts at:      0     759    2136 
   Size:        759    1377
 
BsrFI r'CCGG_y

Cuts at:      0      68    1868    2136 
   Size:         68    1800     268

  Fragments arranged by size: 

                1800     268      68
 
BsrGI T'GTAC_A

Cuts at:      0    2093    2136 
   Size:       2093      43
 
BssSI C'ACGA_G

Cuts at:      0     720    2136 
   Size:        720    1416
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     454    1127    1451    2136 
   Size:        454     673     324     685

  Fragments arranged by size: 

                 685     673     454     324
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     105     466    2136 
   Size:        105     361    1670

  Fragments arranged by size: 

                1670     361     105
 
BstYI r'GATC_y

Cuts at:      0     852    1874    2136 
   Size:        852    1022     262

  Fragments arranged by size: 

                1022     852     262
 
BtsI GCAGTG_nn'

Cuts at:      0     380    1452    1614    2136 
   Size:        380    1072     162     522

  Fragments arranged by size: 

                1072     522     380     162
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1809    1844    2136 
   Size:       1809      35     292

  Fragments arranged by size: 

                1809     292      35
 
EaeI y'GGCC_r

Cuts at:      0    1379    2136 
   Size:       1379     757
 
EarI CTCTTCn'nnn_

Cuts at:      0     178     280     548    1615    1769    2136 
   Size:        178     102     268    1067     154     367

  Fragments arranged by size: 

                1067     367     268     178     154     102
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1887    2131    2136 
   Size:       1887     244       5

  Fragments arranged by size: 

                1887     244       5
 
EcoICRI GAG'CTC

Cuts at:      0     725     879    2136 
   Size:        725     154    1257

  Fragments arranged by size: 

                1257     725     154
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     251     328     394     554     747     865    1720 
   Size:         70     181      77      66     160     193     118     855

Cuts at:   1720    2136 
   Size:        416

  Fragments arranged by size: 

                 855     416     193     181     160     118      77      70
                  66
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      49     343    2136 
   Size:         49     294    1793

  Fragments arranged by size: 

                1793     294      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0     203     336     891    1970    1971    2136 
   Size:        203     133     555    1079       1     165

  Fragments arranged by size: 

                1079     555     203     165     133       1
 
EcoRI G'AATT_C

Cuts at:      0    1855    2136 
   Size:       1855     281
 
HaeII r_GCGC'y

Cuts at:      0    1786    2136 
   Size:       1786     350
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1138    1170    2136 
   Size:       1138      32     966

  Fragments arranged by size: 

                1138     966      32
 
KpnI G_GTAC'C

Cuts at:      0     431    2136 
   Size:        431    1705
 
MscI TGG'CCA

Cuts at:      0    1381    2136 
   Size:       1381     755
 
MslI CAynn'nnrTG

Cuts at:      0     100     103    1582    1899    2039    2136 
   Size:        100       3    1479     317     140      97

  Fragments arranged by size: 

                1479     317     140     100      97       3
 
MspA1I CmG'CkG

Cuts at:      0     154    1242    1985    2136 
   Size:        154    1088     743     151

  Fragments arranged by size: 

                1088     743     154     151
 
NaeI GCC'GGC

Cuts at:      0      70    2136 
   Size:         70    2066
 
NgoMIV G'CCGG_C

Cuts at:      0      68    2136 
   Size:         68    2068
 
NspI r_CATG'y

Cuts at:      0    1087    1212    2038    2136 
   Size:       1087     125     826      98

  Fragments arranged by size: 

                1087     826     125      98
 
PciI A'CATG_T

Cuts at:      0    1208    2034    2136 
   Size:       1208     826     102

  Fragments arranged by size: 

                1208     826     102
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1596    2136 
   Size:       1596     540
 
PfoI T'CCnGG_A

Cuts at:      0    1142    2136 
   Size:       1142     994
 
PpuMI rG'GwC_Cy

Cuts at:      0     203     336     891    2136 
   Size:        203     133     555    1245

  Fragments arranged by size: 

                1245     555     203     133
 
PshAI GACnn'nnGTC

Cuts at:      0    1460    2136 
   Size:       1460     676
 
PspOMI G'GGCC_C

Cuts at:      0    1970    2136 
   Size:       1970     166
 
PspXI vC'TCGA_Gb

Cuts at:      0     886    2136 
   Size:        886    1250
 
PstI C_TGCA'G

Cuts at:      0    1447    1480    1724    1790    2136 
   Size:       1447      33     244      66     346

  Fragments arranged by size: 

                1447     346     244      66      33
 
PvuII CAG'CTG

Cuts at:      0     154    1242    2136 
   Size:        154    1088     894

  Fragments arranged by size: 

                1088     894     154
 
SacI G_AGCT'C

Cuts at:      0     727     881    2136 
   Size:        727     154    1255

  Fragments arranged by size: 

                1255     727     154
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1769    2136 
   Size:       1769     367
 
SfcI C'TryA_G

Cuts at:      0    1443    1476    1720    1786    2004    2136 
   Size:       1443      33     244      66     218     132

  Fragments arranged by size: 

                1443     244     218     132      66      33
 
SgrAI Cr'CCGG_yG

Cuts at:      0    1868    2136 
   Size:       1868     268
 
SmaI CCC'GGG

Cuts at:      0    1159    2136 
   Size:       1159     977
 
SmlI C'TyrA_G

Cuts at:      0     886    2136 
   Size:        886    1250
 
SphI G_CATG'C

Cuts at:      0    1087    2136 
   Size:       1087    1049
 
SspI AAT'ATT

Cuts at:      0    1268    2136 
   Size:       1268     868
 
StuI AGG'CCT

Cuts at:      0     349    1441    2136 
   Size:        349    1092     695

  Fragments arranged by size: 

                1092     695     349
 
StyI C'CwwG_G

Cuts at:      0     465     495     687    2136 
   Size:        465      30     192    1449

  Fragments arranged by size: 

                1449     465     192      30
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1163    1742    1759    2136 
   Size:       1163     579      17     377

  Fragments arranged by size: 

                1163     579     377      17
 
TatI w'GTAC_w

Cuts at:      0    2093    2136 
   Size:       2093      43
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     630     645     662     677    1483    1515    1993    2025 
   Size:        630      15      17      15     806      32     478      32

Cuts at:   2025    2136 
   Size:        111

  Fragments arranged by size: 

                 806     630     478     111      32      32      17      15
                  15
 
XbaI T'CTAG_A

Cuts at:      0    1053    1566    2136 
   Size:       1053     513     570

  Fragments arranged by size: 

                1053     570     513
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     207    2136 
   Size:        207    1929
 
XhoI C'TCGA_G

Cuts at:      0     886    2136 
   Size:        886    1250
 
XmaI C'CCGG_G

Cuts at:      0    1157    2136 
   Size:       1157     979
 
XmnI GAAnn'nnTTC

Cuts at:      0     172    2136 
   Size:        172    1964
 
ZraI GAC'GTC

Cuts at:      0      93    2136 
   Size:         93    2043
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AcuI   AflIII     AleI    AlwNI     ApaI
    ApaLI     ApoI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbsI    BbvCI     BcgI     BclI     BglI     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I     BsaI    BsaHI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstXI    BstYI     BtsI    CspCI
     EaeI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    HaeII    Hin4I     KpnI     MscI     MslI   MspA1I     NaeI   NgoMIV
     NspI     PciI    PflMI     PfoI    PpuMI    PshAI   PspOMI    PspXI
     PstI    PvuII     SacI     SapI     SfcI    SgrAI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AclI     AfeI    AflII     AgeI     AhdI     AlfI     AloI
     AscI     AseI    AsiSI     BbeI    BciVI    BfrBI    BglII     BmtI
    BpuEI    BsaAI    BsaBI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI    EcoRV     FalI     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KasI     MfeI     MluI
     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PmlI     PpiI     PsiI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SnaBI     SpeI     SrfI     SwaI  Tth111I
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