Restriction map for pF1KSDA0326
##################################
# NO CUT ENZYMES (  101 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcyI        
AfaI        AflIII      AhdI        AlwNI       AocI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AviII       BalI        BbeI        BbrPI       
BciVI       BclI        BglII       BlpI        Bpu1102I    
BsaAI       BsaHI       BsiWI       BsmBI       Bsp1407I    
BspDI       BspLU11I    BsrDI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        EagI        
Eam1105I    EclXI       Eco52I      Eco81I      EcoO65I     
EcoRV       EcoT22I     FbaI        FseI        FspI        
Hin1I       HincII      HindII      HpaI        KasI        
KpnI        MfeI        MluI        MluNI       MscI        
MunI        NarI        NdeI        NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RsaI        
RsrII       SalI        SbfI        ScaI        SfoI        
SfuI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        


###################################
# ONE CUT ENZYMES (   48 enzymes) #
###################################

AccIII         42  TCCGGA               T^CCGGA
AcsI         1869  GAATTC               [GA]^AATT[CT]
AgeI         1357  ACCGGT               A^CCGGT
Alw44I        705  GTGCAC               G^TGCAC
Aor51HI       890  AGCGCT               AGC^GCT
ApaLI         705  GTGCAC               G^TGCAC
ApoI         1869  GAATTC               [GA]^AATT[CT]
AvrII        2094  CCTAGG               C^CTAGG
BamHI        1766  GGATCC               G^GATCC
BbsI         1617  GAAGAC               GAAGAC(2/6)
BbvCI        2172  CCTCAGC              CCTCAGC(-5/-2)
BcgI         1055  GCAAGAGCTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          325  GCCTGCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         2094  CCTAGG               C^CTAGG
BpuAI        1617  GAAGAC               GAAGAC(2/6)
BsaBI        1767  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI          42  TCCGGA               T^CCGGA
BsiEI        2334  CGACCG               CG[GA][CT]^CG
BsmI         2152  GAATGC               GAATGC(1/-1)
BspEI          42  TCCGGA               T^CCGGA
BspHI        1319  TCATGA               T^CATGA
DraI         2140  TTTAAA               TTT^AAA
DrdI          205  GACCCCTCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III      890  AGCGCT               AGC^GCT
EcoRI        1869  GAATTC               G^AATTC
HgaI         1235  GCGTC                GACGC(5/10)
HindIII      2163  AAGCTT               A^AGCTT
KspI         1272  CCGCGG               CCGC^GG
MamI         1767  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI           42  TCCGGA               T^CCGGA
NaeI         2545  GCCGGC               GCC^GGC
NcoI         2478  CCATGG               C^CATGG
NgoMIV       2545  GCCGGC               G^CCGGC
PflMI        1578  CCACGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI        1357  ACCGGT               A^CCGGT
PstI           34  CTGCAG               CTGCA^G
RcaI         1319  TCATGA               T^CATGA
SacII        1272  CCGCGG               CCGC^GG
SfcI           34  CTGCAG               C^T[GA][CT]AG
SfiI          324  GGCCTGCGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI         1879  CCCGGG               CCC^GGG
TaqI         2333  TCGA                 T^CGA
TfiI          160  GAATC                G^A[AT]TC
TthHB8I      2333  TCGA                 T^CGA
Van91I       1578  CCACGAGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         2498  CCAAAACCCTAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI        1879  CCCGGG               C^CCGGG
XmaI         1879  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

AflII        1921  CTTAAG               C^TTAAG
             2124  CTTAAG              
BfrI         1921  CTTAAG               C^TTAAG
             2124  CTTAAG              
BsaI          140  GAGACC               GGTCTC(1/5)
             2449  GAGACC              
BsaWI          42  TCCGGA               [AT]^CCGG[AT]
             1357  ACCGGT              
BspMI         535  ACCTGC               ACCTGC(4/8)
             1103  ACCTGC              
BssSI        1579  CACGAG               CACGAG(-5/-1)
             1714  CTCGTG              
BstXI         248  CCAATCCCTTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              307  CCATCTGACTGG        
EaeI          897  CGGCCA               [CT]^GGCC[GA]
             1065  CGGCCA              
EcoNI        1104  CCTGCGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1858  CCTTTCAGAGG         
MaeII        1162  ACGT                 A^CGT
             1511  ACGT                
PvuII         336  CAGCTG               CAG^CTG
              645  CAGCTG              
SexAI         575  ACCTGGT              A^CC[AT]GGT
              659  ACCTGGT             
StuI          323  AGGCCT               AGG^CCT
             1562  AGGCCT              
Tsp45I        242  GTCAC                ^GT[GC]AC
             2416  GTCAC               
Tsp509I      1870  AATT                 ^AATT
             2088  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AccII       AciI        AluI        AlwI        ApaI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       BsoBI       
Bsp1286I    BsrBI       BsrFI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr10I      Cfr13I      DdeI        
DpnII       DraII       DraIII      DsaI        EarI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PleI        PpuMI       
SacI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SgrAI       SmlI        StyI        Tru9I       
TseI        TspRI       XhoII       XmnI        
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com