Restriction map for
pF1KSDA0319
(Circular) (Six-base) MAPSORT of: pF1KSDA0319.seq Check: 1572 from: 1 to: 6355
pF1KSDA0319 6355 bp
With 182 enzymes: SgfI *
June 20, 2008 10:33 ..
AatII G_ACGT'C
Cuts at: 1477 1477
Size: 6355
AccI GT'mk_AC
Cuts at: 290 1951 2353 3391 290
Size: 1661 402 1038 3254
Fragments arranged by size:
3254 1661 1038 402
Acc65I G'GTAC_C
Cuts at: 3369 3369
Size: 6355
AclI AA'CG_TT
Cuts at: 6195 6195
Size: 6355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 839 1207 1556 1942 2795 2890 3231 3303 4178
Size: 368 349 386 853 95 341 72 875
Cuts at: 4178 4610 5359 5627 839
Size: 432 749 268 1567
Fragments arranged by size:
1567 875 853 749 432 386 368 349
341 268 95 72
AfeI AGC'GCT
Cuts at: 2911 2911
Size: 6355
AflIII A'CryG_T
Cuts at: 1845 2304 3705 4811 5788 1845
Size: 459 1401 1106 977 2412
Fragments arranged by size:
2412 1401 1106 977 459
AgeI A'CCGG_T
Cuts at: 4743 4743
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 1333 1333
Size: 6355
AleI CACnn'nnGTG
Cuts at: 142 430 2635 142
Size: 288 2205 3862
Fragments arranged by size:
3862 2205 288
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 481 513 911 943 481
Size: 32 398 32 5893
Fragments arranged by size:
5893 398 32 32
AlwNI CAG_nnn'CTG
Cuts at: 465 2005 2226 2413 2533 3658 5227 465
Size: 1540 221 187 120 1125 1569 1593
Fragments arranged by size:
1593 1569 1540 1125 221 187 120
ApaI G_GGCC'C
Cuts at: 409 1553 2703 409
Size: 1144 1150 4061
Fragments arranged by size:
4061 1150 1144
ApaLI G'TGCA_C
Cuts at: 5125 5125
Size: 6355
ApoI r'AATT_y
Cuts at: 1242 3357 5708 5964 1242
Size: 2115 2351 256 1633
Fragments arranged by size:
2351 2115 1633 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 6355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6355
AvaI C'yCGr_G
Cuts at: 504 1014 2288 3373 504
Size: 510 1274 1085 3486
Fragments arranged by size:
3486 1274 1085 510
AvrII C'CTAG_G
Cuts at: 2560 2560
Size: 6355
BamHI G'GATC_C
Cuts at: 2387 3378 2387
Size: 991 5364
BanI G'GyrC_C
Cuts at: 129 738 3369 3783 4031 4066 129
Size: 609 2631 414 248 35 2418
Fragments arranged by size:
2631 2418 609 414 248 35
BanII G_rGCy'C
Cuts at: 395 409 499 653 770 971 1460 1553 2289
Size: 14 90 154 117 201 489 93 736
Cuts at: 2289 2703 3367 4397 395
Size: 414 664 1030 2353
Fragments arranged by size:
2353 1030 736 664 489 414 201 154
117 93 90 14
BbeI G_GCGC'C
Cuts at: 133 3787 4035 133
Size: 3654 248 2453
Fragments arranged by size:
3654 2453 248
BbsI GAAGACnn'nnnn_
Cuts at: 940 1329 1573 2085 940
Size: 389 244 512 5210
Fragments arranged by size:
5210 512 389 244
BciVI GTATCCnnnnn_n'
Cuts at: 3219 4251 5014 6355 3219
Size: 1032 763 1341 3219
Fragments arranged by size:
3219 1341 1032 763
BclI T'GATC_A
Cuts at: 3873 3873
Size: 6355
BglII A'GATC_T
Cuts at: 3868 5907 3868
Size: 2039 4316
BlpI GC'TnA_GC
Cuts at: 970 3465 970
Size: 2495 3860
Bme1580I G_kGCm'C
Cuts at: 189 409 1553 2703 3978 4071 5129 189
Size: 220 1144 1150 1275 93 1058 1415
Fragments arranged by size:
1415 1275 1150 1144 1058 220 93
BmgBI CAC'GTC
Cuts at: 2112 2237 2112
Size: 125 6230
BmrI ACTGGGnnnn_n'
Cuts at: 629 687 991 2032 2112 2117 3565 3646 3981
Size: 58 304 1041 80 5 1448 81 335
Cuts at: 3981 6127 629
Size: 2146 857
Fragments arranged by size:
2146 1448 1041 857 335 304 81 80
58 5
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 990 3210 4720 990
Size: 2220 1510 2625
Fragments arranged by size:
2625 2220 1510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 877 985 1163 1982 2161 2435 3180 634
Size: 243 108 178 819 179 274 745 3809
Fragments arranged by size:
3809 819 745 274 243 179 178 108
Bpu10I CC'TnA_GC
Cuts at: 764 764
Size: 6355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2862 3529 4408 4902 5200 2862
Size: 667 879 494 298 4017
Fragments arranged by size:
4017 879 667 494 298
BsaI GGTCTCn'nnnn_
Cuts at: 2179 6059 2179
Size: 3880 2475
BsaAI yAC'GTr
Cuts at: 3345 3706 3345
Size: 361 5994
BsaBI GATnn'nnATC
Cuts at: 6 3867 5906 6
Size: 3861 2039 455
Fragments arranged by size:
3861 2039 455
BsaHI Gr'CG_yC
Cuts at: 130 1474 1870 3784 4032 4736 6232 130
Size: 1344 396 1914 248 704 1496 253
Fragments arranged by size:
1914 1496 1344 704 396 253 248
BsaWI w'CCGG_w
Cuts at: 3542 4063 4743 5017 5164 5575 3542
Size: 521 680 274 147 411 4322
Fragments arranged by size:
4322 680 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 481 511 1092 1122 2464 2494 481
Size: 30 581 30 1342 30 4342
Fragments arranged by size:
4342 1342 581 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 592 705 592
Size: 113 6242
BseYI C'CCAG_C
Cuts at: 3778 4536 5115 5611 3778
Size: 758 579 496 4522
Fragments arranged by size:
4522 758 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 279 440 2147 2569 2776 219
Size: 60 161 1707 422 207 3798
Fragments arranged by size:
3798 1707 422 207 161 60
BsiEI CG_ry'CG
Cuts at: 104 302 3895 3941 5151 104
Size: 198 3593 46 1210 1308
Fragments arranged by size:
3593 1308 1210 198 46
BsiHKAI G_wGCw'C
Cuts at: 149 437 770 971 1004 1031 1460 2111 3191
Size: 288 333 201 33 27 429 651 1080
Cuts at: 3191 3367 4145 4335 5129 149
Size: 176 778 190 794 1375
Fragments arranged by size:
1375 1080 794 778 651 429 333 288
201 190 176 33 27
BsmBI CGTCTCn'nnnn_
Cuts at: 742 742
Size: 6355
Bsp1286I G_dGCh'C
Cuts at: 149 189 395 409 437 499 653 770 971
Size: 40 206 14 28 62 154 117 201
Cuts at: 971 1004 1031 1460 1553 2111 2289 2703 3191
Size: 33 27 429 93 558 178 414 488
Cuts at: 3191 3367 3978 4071 4145 4335 4397 5129 149
Size: 176 611 93 74 190 62 732 1375
Fragments arranged by size:
1375 732 611 558 488 429 414 206
201 190 178 176 154 117 93 93
74 62 62 40 33 28 27 14
BspEI T'CCGG_A
Cuts at: 5575 5575
Size: 6355
BspHI T'CATG_A
Cuts at: 5531 5531
Size: 6355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 169 1427 2583 2848 3403 3617 3919 4300 169
Size: 1258 1156 265 555 214 302 381 2224
Fragments arranged by size:
2224 1258 1156 555 381 302 265 214
BsrBI CCG'CTC
Cuts at: 50 4645 4699 6185 6350 50
Size: 4595 54 1486 165 55
Fragments arranged by size:
4595 1486 165 55 54
BsrDI GCAATG_nn'
Cuts at: 154 1898 4265 154
Size: 1744 2367 2244
Fragments arranged by size:
2367 2244 1744
BsrFI r'CCGG_y
Cuts at: 2011 4351 4532 4743 2011
Size: 2340 181 211 3623
Fragments arranged by size:
3623 2340 211 181
BsrGI T'GTAC_A
Cuts at: 2480 2736 2480
Size: 256 6099
BssSI C'ACGA_G
Cuts at: 4624 4984 4624
Size: 360 5995
BstAPI GCAn_nnn'nTGC
Cuts at: 184 3062 3730 184
Size: 2878 668 2809
Fragments arranged by size:
2878 2809 668
BstBI TT'CG_AA
Cuts at: 110 110
Size: 6355
BstXI CCAn_nnnn'nTGG
Cuts at: 402 2130 2337 3204 402
Size: 1728 207 867 3553
Fragments arranged by size:
3553 1728 867 207
BstYI r'GATC_y
Cuts at: 1 509 2387 2709 3378 3845 3868 4203 4449
Size: 508 1878 322 669 467 23 335 246
Cuts at: 4449 5463 5549 5561 5907 1
Size: 1014 86 12 346 449
Fragments arranged by size:
1878 1014 669 508 467 449 346 335
322 246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 527 527
Size: 6355
BtgI C'CryG_G
Cuts at: 125 983 2082 2883 125
Size: 858 1099 801 3597
Fragments arranged by size:
3597 1099 858 801
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2090 4303 4484 4647 2090
Size: 2213 181 163 3798
Fragments arranged by size:
3798 2213 181 163
BtsI GCAGTG_nn'
Cuts at: 202 1103 1791 2135 2531 2889 3596 202
Size: 901 688 344 396 358 707 2961
Fragments arranged by size:
2961 901 707 688 396 358 344
ClaI AT'CG_AT
Cuts at: 2426 2426
Size: 6355
DraI TTT'AAA
Cuts at: 3352 3352
Size: 6355
DraIII CAC_nnn'GTG
Cuts at: 2445 2445
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 1585 2683 4059 4919 1585
Size: 1098 1376 860 3021
Fragments arranged by size:
3021 1376 1098 860
EaeI y'GGCC_r
Cuts at: 299 2009 2397 2880 3938 4112 4503 4530 5877
Size: 1710 388 483 1058 174 391 27 1347
Cuts at: 5877 299
Size: 777
Fragments arranged by size:
1710 1347 1058 777 483 391 388 174
27
EagI C'GGCC_G
Cuts at: 299 3938 299
Size: 3639 2716
EarI CTCTTCn'nnn_
Cuts at: 156 1404 1556 2560 4376 4586 156
Size: 1248 152 1004 1816 210 1925
Fragments arranged by size:
1925 1816 1248 1004 210 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 4561 4873 5019 6165 4561
Size: 312 146 1146 4751
Fragments arranged by size:
4751 1146 312 146
EcoICRI GAG'CTC
Cuts at: 768 969 1458 3365 768
Size: 201 489 1907 3758
Fragments arranged by size:
3758 1907 489 201
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 634 839 877 985 1163 1207 1556 1942 1982
Size: 205 38 108 178 44 349 386 40
Cuts at: 1982 2161 2435 2795 2890 3180 3231 3303 4178
Size: 179 274 360 95 290 51 72 875
Cuts at: 4178 4610 5359 5627 634
Size: 432 749 268 1362
Fragments arranged by size:
1362 875 749 432 386 360 349 290
274 268 205 179 178 108 95 72
51 44 40 38
EcoO109I rG'GnC_Cy
Cuts at: 406 570 621 681 704 759 1550 1825 2119
Size: 164 51 60 23 55 791 275 294
Cuts at: 2119 2186 2516 3492 406
Size: 67 330 976 3269
Fragments arranged by size:
3269 976 791 330 294 275 164 67
60 55 51 23
EcoRI G'AATT_C
Cuts at: 3357 3357
Size: 6355
EcoRV GAT'ATC
Cuts at: 534 534
Size: 6355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 642 2838 4372 642
Size: 2196 1534 2625
Fragments arranged by size:
2625 2196 1534
FseI GG_CCGG'CC
Cuts at: 2015 2015
Size: 6355
FspI TGC'GCA
Cuts at: 4134 4134
Size: 6355
HaeII r_GCGC'y
Cuts at: 133 1210 2913 3787 4035 5059 6038 133
Size: 1077 1703 874 248 1024 979 450
Fragments arranged by size:
1703 1077 1024 979 874 450 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 568 600 1319 1351 2408 2440 3258 3290 4195
Size: 32 719 32 1057 32 818 32 905
Cuts at: 4195 4225 4227 4257 568
Size: 30 2 30 2666
Fragments arranged by size:
2666 1057 905 818 719 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1416 1578 1634 3392 1416
Size: 162 56 1758 4379
Fragments arranged by size:
4379 1758 162 56
KasI G'GCGC_C
Cuts at: 129 3783 4031 129
Size: 3654 248 2453
Fragments arranged by size:
3654 2453 248
KpnI G_GTAC'C
Cuts at: 3373 3373
Size: 6355
MfeI C'AATT_G
Cuts at: 173 1315 173
Size: 1142 5213
MluI A'CGCG_T
Cuts at: 5788 5788
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 702 848 1305 5026 5210 702
Size: 146 457 3721 184 1847
Fragments arranged by size:
3721 1847 457 184 146
MscI TGG'CCA
Cuts at: 2399 2882 4114 2399
Size: 483 1232 4640
Fragments arranged by size:
4640 1232 483
MslI CAynn'nnrTG
Cuts at: 142 265 430 2635 4342 4469 142
Size: 123 165 2205 1707 127 2028
Fragments arranged by size:
2205 2028 1707 165 127 123
MspA1I CmG'CkG
Cuts at: 323 353 462 745 1920 2413 2610 2622 2646
Size: 30 109 283 1175 493 197 12 24
Cuts at: 2646 3464 3778 4138 5153 5398 323
Size: 818 314 360 1015 245 1280
Fragments arranged by size:
1280 1175 1015 818 493 360 314 283
245 197 109 30 24 12
NaeI GCC'GGC
Cuts at: 2013 4534 2013
Size: 2521 3834
NarI GG'CG_CC
Cuts at: 130 3784 4032 130
Size: 3654 248 2453
Fragments arranged by size:
3654 2453 248
NcoI C'CATG_G
Cuts at: 125 2883 125
Size: 2758 3597
NgoMIV G'CCGG_C
Cuts at: 2011 4532 2011
Size: 2521 3834
NruI TCG'CGA
Cuts at: 4716 4716
Size: 6355
NspI r_CATG'y
Cuts at: 1849 2308 2799 3406 3906 4815 1849
Size: 459 491 607 500 909 3389
Fragments arranged by size:
3389 909 607 500 491 459
PasI CC'CwG_GG
Cuts at: 1821 1821
Size: 6355
PciI A'CATG_T
Cuts at: 1845 2304 4811 1845
Size: 459 2507 3389
Fragments arranged by size:
3389 2507 459
PflMI CCAn_nnn'nTGG
Cuts at: 2413 2445 2413
Size: 32 6323
PfoI T'CCnGG_A
Cuts at: 893 2390 3116 893
Size: 1497 726 4132
Fragments arranged by size:
4132 1497 726
PmeI GTTT'AAAC
Cuts at: 3352 3352
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 481 513 5494 5526 481
Size: 32 4981 32 1310
Fragments arranged by size:
4981 1310 32 32
PpuMI rG'GwC_Cy
Cuts at: 621 759 1825 2186 621
Size: 138 1066 361 4790
Fragments arranged by size:
4790 1066 361 138
PsiI TTA'TAA
Cuts at: 1159 1159
Size: 6355
PspOMI G'GGCC_C
Cuts at: 405 1549 2699 405
Size: 1144 1150 4061
Fragments arranged by size:
4061 1150 1144
PstI C_TGCA'G
Cuts at: 1677 2456 2594 3400 4085 1677
Size: 779 138 806 685 3947
Fragments arranged by size:
3947 806 779 685 138
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6355
PvuII CAG'CTG
Cuts at: 323 462 1920 2413 2610 2646 3778 4138 323
Size: 139 1458 493 197 36 1132 360 2540
Fragments arranged by size:
2540 1458 1132 493 360 197 139 36
RsrII CG'GwC_CG
Cuts at: 4548 4548
Size: 6355
SacI G_AGCT'C
Cuts at: 770 971 1460 3367 770
Size: 201 489 1907 3758
Fragments arranged by size:
3758 1907 489 201
SalI G'TCGA_C
Cuts at: 3390 3390
Size: 6355
SapI GCTCTTCn'nnn_
Cuts at: 4376 4586 4376
Size: 210 6145
SbfI CC_TGCA'GG
Cuts at: 2594 3400 2594
Size: 806 5549
ScaI AGT'ACT
Cuts at: 5872 5872
Size: 6355
SexAI A'CCwGG_T
Cuts at: 359 359
Size: 6355
SfcI C'TryA_G
Cuts at: 33 1214 1427 1620 1673 1958 2452 2590 3396
Size: 1181 213 193 53 285 494 138 806
Cuts at: 3396 4081 5076 5267 33
Size: 685 995 191 1121
Fragments arranged by size:
1181 1121 995 806 685 494 285 213
193 191 138 53
SfoI GGC'GCC
Cuts at: 131 3785 4033 131
Size: 3654 248 2453
Fragments arranged by size:
3654 2453 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6355
SmaI CCC'GGG
Cuts at: 1016 2290 3375 1016
Size: 1274 1085 3996
Fragments arranged by size:
3996 1274 1085
SmlI C'TyrA_G
Cuts at: 2841 3508 4423 4917 5179 2841
Size: 667 915 494 262 4017
Fragments arranged by size:
4017 915 667 494 262
SnaBI TAC'GTA
Cuts at: 3345 3345
Size: 6355
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6355
SphI G_CATG'C
Cuts at: 3406 3906 3406
Size: 500 5855
SrfI GCCC'GGGC
Cuts at: 2290 2290
Size: 6355
SspI AAT'ATT
Cuts at: 2145 2145
Size: 6355
StuI AGG'CCT
Cuts at: 453 1192 453
Size: 739 5616
StyI C'CwwG_G
Cuts at: 125 1269 2560 2883 3487 3841 125
Size: 1144 1291 323 604 354 2639
Fragments arranged by size:
2639 1291 1144 604 354 323
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 3936 4069 4528 4739 3936
Size: 133 459 211 5552
Fragments arranged by size:
5552 459 211 133
TatI w'GTAC_w
Cuts at: 668 1021 1501 1863 2480 2736 3107 5870 668
Size: 353 480 362 617 256 371 2763 1153
Fragments arranged by size:
2763 1153 617 480 371 362 353 256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5872 5904 5872
Size: 32 6323
Tth111I GACn'n_nGTC
Cuts at: 4150 4150
Size: 6355
XbaI T'CTAG_A
Cuts at: 3384 3384
Size: 6355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 327 2247 3848 327
Size: 1920 1601 2834
Fragments arranged by size:
2834 1920 1601
XmaI C'CCGG_G
Cuts at: 1014 2288 3373 1014
Size: 1274 1085 3996
Fragments arranged by size:
3996 1274 1085
ZraI GAC'GTC
Cuts at: 1475 1475
Size: 6355
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BciVI BclI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI
HaeII Hin4I HincII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NruI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AflII AlfI AscI BaeI BbvCI BcgI BfrBI
BglI BmtI BsiWI BsmI BssHII BstEII BstZ17I CspCI
EcoNI FspAI HindIII HpaI NdeI NheI NotI NsiI
PacI PmlI PshAI PspXI PsrI SacII SanDI SfiI
SgrAI SwaI XhoI XmnI