Restriction map for pF1KSDA0319
 (Linear) (Six-base) MAPSORT of: KIAA0319.seq  Check: 9942  from: 1  to: 3216



KIAA0319.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AatII G_ACGT'C

Cuts at:      0    1351    3216 
   Size:       1351    1865
 
AccI GT'mk_AC

Cuts at:      0     164    1825    2227    3216 
   Size:        164    1661     402     989

  Fragments arranged by size: 

                1661     989     402     164
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     713    1081    1430    1816    2669    2764    3105    3177 
   Size:        713     368     349     386     853      95     341      72

Cuts at:   3177    3216 
   Size:         39

  Fragments arranged by size: 

                 853     713     386     368     349     341      95      72
                  39
 
AfeI AGC'GCT

Cuts at:      0    2785    3216 
   Size:       2785     431
 
AflIII A'CryG_T

Cuts at:      0    1719    2178    3216 
   Size:       1719     459    1038

  Fragments arranged by size: 

                1719    1038     459
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1207    3216 
   Size:       1207    2009
 
AleI CACnn'nnGTG

Cuts at:      0      16     304    2509    3216 
   Size:         16     288    2205     707

  Fragments arranged by size: 

                2205     707     288      16
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     355     387     785     817    3216 
   Size:        355      32     398      32    2399

  Fragments arranged by size: 

                2399     398     355      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     339    1879    2100    2287    2407    3216 
   Size:        339    1540     221     187     120     809

  Fragments arranged by size: 

                1540     809     339     221     187     120
 
ApaI G_GGCC'C

Cuts at:      0     283    1427    2577    3216 
   Size:        283    1144    1150     639

  Fragments arranged by size: 

                1150    1144     639     283
 
ApoI r'AATT_y

Cuts at:      0    1116    3216 
   Size:       1116    2100
 
AvaI C'yCGr_G

Cuts at:      0     378     888    2162    3216 
   Size:        378     510    1274    1054

  Fragments arranged by size: 

                1274    1054     510     378
 
AvrII C'CTAG_G

Cuts at:      0    2434    3216 
   Size:       2434     782
 
BamHI G'GATC_C

Cuts at:      0    2261    3216 
   Size:       2261     955
 
BanI G'GyrC_C

Cuts at:      0       3     612    3216 
   Size:          3     609    2604

  Fragments arranged by size: 

                2604     609       3
 
BanII G_rGCy'C

Cuts at:      0     269     283     373     527     644     845    1334    1427 
   Size:        269      14      90     154     117     201     489      93

Cuts at:   1427    2163    2577    3216 
   Size:        736     414     639

  Fragments arranged by size: 

                 736     639     489     414     269     201     154     117
                  93      90      14
 
BbeI G_GCGC'C

Cuts at:      0       7    3216 
   Size:          7    3209
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     814    1203    1447    1959    3216 
   Size:        814     389     244     512    1257

  Fragments arranged by size: 

                1257     814     512     389     244
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    3093    3216 
   Size:       3093     123
 
BlpI GC'TnA_GC

Cuts at:      0     844    3216 
   Size:        844    2372
 
Bme1580I G_kGCm'C

Cuts at:      0      63     283    1427    2577    3216 
   Size:         63     220    1144    1150     639

  Fragments arranged by size: 

                1150    1144     639     220      63
 
BmgBI CAC'GTC

Cuts at:      0    1986    2111    3216 
   Size:       1986     125    1105

  Fragments arranged by size: 

                1986    1105     125
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     503     561     865    1906    1986    1991    3216 
   Size:        503      58     304    1041      80       5    1225

  Fragments arranged by size: 

                1225    1041     503     304      80      58       5
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     864    3084    3216 
   Size:        864    2220     132

  Fragments arranged by size: 

                2220     864     132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     751     859    1037    1856    2035    2309    3054 
   Size:        508     243     108     178     819     179     274     745

Cuts at:   3054    3216 
   Size:        162

  Fragments arranged by size: 

                 819     745     508     274     243     179     178     162
                 108
 
Bpu10I CC'TnA_GC

Cuts at:      0     638    3216 
   Size:        638    2578
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2736    3216 
   Size:       2736     480
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2053    3216 
   Size:       2053    1163
 
BsaHI Gr'CG_yC

Cuts at:      0       4    1348    1744    3216 
   Size:          4    1344     396    1472

  Fragments arranged by size: 

                1472    1344     396       4
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     355     385     966     996    2338    2368    3216 
   Size:        355      30     581      30    1342      30     848

  Fragments arranged by size: 

                1342     848     581     355      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     466     579    3216 
   Size:        466     113    2637

  Fragments arranged by size: 

                2637     466     113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     153     314    2021    2443    2650    3216 
   Size:         93      60     161    1707     422     207     566

  Fragments arranged by size: 

                1707     566     422     207     161      93      60
 
BsiEI CG_ry'CG

Cuts at:      0     176    3216 
   Size:        176    3040
 
BsiHKAI G_wGCw'C

Cuts at:      0      23     311     644     845     878     905    1334    1985 
   Size:         23     288     333     201      33      27     429     651

Cuts at:   1985    3065    3216 
   Size:       1080     151

  Fragments arranged by size: 

                1080     651     429     333     288     201     151      33
                  27      23
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     616    3216 
   Size:        616    2600
 
Bsp1286I G_dGCh'C

Cuts at:      0      23      63     269     283     311     373     527     644 
   Size:         23      40     206      14      28      62     154     117

Cuts at:    644     845     878     905    1334    1427    1985    2163    2577 
   Size:        201      33      27     429      93     558     178     414

Cuts at:   2577    3065    3216 
   Size:        488     151

  Fragments arranged by size: 

                 558     488     429     414     206     201     178     154
                 151     117      93      62      40      33      28      27
                  23      14
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      43    1301    2457    2722    3216 
   Size:         43    1258    1156     265     494

  Fragments arranged by size: 

                1258    1156     494     265      43
 
BsrDI GCAATG_nn'

Cuts at:      0      28    1772    3216 
   Size:         28    1744    1444

  Fragments arranged by size: 

                1744    1444      28
 
BsrFI r'CCGG_y

Cuts at:      0    1885    3216 
   Size:       1885    1331
 
BsrGI T'GTAC_A

Cuts at:      0    2354    2610    3216 
   Size:       2354     256     606

  Fragments arranged by size: 

                2354     606     256
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      58    2936    3216 
   Size:         58    2878     280

  Fragments arranged by size: 

                2878     280      58
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     276    2004    2211    3078    3216 
   Size:        276    1728     207     867     138

  Fragments arranged by size: 

                1728     867     276     207     138
 
BstYI r'GATC_y

Cuts at:      0     383    2261    2583    3216 
   Size:        383    1878     322     633

  Fragments arranged by size: 

                1878     633     383     322
 
Bsu36I CC'TnA_GG

Cuts at:      0     401    3216 
   Size:        401    2815
 
BtgI C'CryG_G

Cuts at:      0     857    1956    2757    3216 
   Size:        857    1099     801     459

  Fragments arranged by size: 

                1099     857     801     459
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1964    3216 
   Size:       1964    1252
 
BtsI GCAGTG_nn'

Cuts at:      0      76     977    1665    2009    2405    2763    3216 
   Size:         76     901     688     344     396     358     453

  Fragments arranged by size: 

                 901     688     453     396     358     344      76
 
ClaI AT'CG_AT

Cuts at:      0    2300    3216 
   Size:       2300     916
 
DraIII CAC_nnn'GTG

Cuts at:      0    2319    3216 
   Size:       2319     897
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1459    2557    3216 
   Size:       1459    1098     659

  Fragments arranged by size: 

                1459    1098     659
 
EaeI y'GGCC_r

Cuts at:      0     173    1883    2271    2754    3216 
   Size:        173    1710     388     483     462

  Fragments arranged by size: 

                1710     483     462     388     173
 
EagI C'GGCC_G

Cuts at:      0     173    3216 
   Size:        173    3043
 
EarI CTCTTCn'nnn_

Cuts at:      0      30    1278    1430    2434    3216 
   Size:         30    1248     152    1004     782

  Fragments arranged by size: 

                1248    1004     782     152      30
 
EcoICRI GAG'CTC

Cuts at:      0     642     843    1332    3216 
   Size:        642     201     489    1884

  Fragments arranged by size: 

                1884     642     489     201
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     508     713     751     859    1037    1081    1430    1816 
   Size:        508     205      38     108     178      44     349     386

Cuts at:   1816    1856    2035    2309    2669    2764    3054    3105    3177 
   Size:         40     179     274     360      95     290      51      72

Cuts at:   3177    3216 
   Size:         39

  Fragments arranged by size: 

                 508     386     360     349     290     274     205     179
                 178     108      95      72      51      44      40      39
                  38
 
EcoO109I rG'GnC_Cy

Cuts at:      0     280     444     495     555     578     633    1424    1699 
   Size:        280     164      51      60      23      55     791     275

Cuts at:   1699    1993    2060    2390    3216 
   Size:        294      67     330     826

  Fragments arranged by size: 

                 826     791     330     294     280     275     164      67
                  60      55      51      23
 
EcoRV GAT'ATC

Cuts at:      0     408    3216 
   Size:        408    2808
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     516    2712    3216 
   Size:        516    2196     504

  Fragments arranged by size: 

                2196     516     504
 
FseI GG_CCGG'CC

Cuts at:      0    1889    3216 
   Size:       1889    1327
 
HaeII r_GCGC'y

Cuts at:      0       7    1084    2787    3216 
   Size:          7    1077    1703     429

  Fragments arranged by size: 

                1703    1077     429       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     442     474    1193    1225    2282    2314    3132    3164 
   Size:        442      32     719      32    1057      32     818      32

Cuts at:   3164    3216 
   Size:         52

  Fragments arranged by size: 

                1057     818     719     442      52      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1290    1452    1508    3216 
   Size:       1290     162      56    1708

  Fragments arranged by size: 

                1708    1290     162      56
 
KasI G'GCGC_C

Cuts at:      0       3    3216 
   Size:          3    3213
 
MfeI C'AATT_G

Cuts at:      0      47    1189    3216 
   Size:         47    1142    2027

  Fragments arranged by size: 

                2027    1142      47
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     576     722    1179    3216 
   Size:        576     146     457    2037

  Fragments arranged by size: 

                2037     576     457     146
 
MscI TGG'CCA

Cuts at:      0    2273    2756    3216 
   Size:       2273     483     460

  Fragments arranged by size: 

                2273     483     460
 
MslI CAynn'nnrTG

Cuts at:      0      16     139     304    2509    3216 
   Size:         16     123     165    2205     707

  Fragments arranged by size: 

                2205     707     165     123      16
 
MspA1I CmG'CkG

Cuts at:      0     197     227     336     619    1794    2287    2484    2496 
   Size:        197      30     109     283    1175     493     197      12

Cuts at:   2496    2520    3216 
   Size:         24     696

  Fragments arranged by size: 

                1175     696     493     283     197     197     109      30
                  24      12
 
NaeI GCC'GGC

Cuts at:      0    1887    3216 
   Size:       1887    1329
 
NarI GG'CG_CC

Cuts at:      0       4    3216 
   Size:          4    3212
 
NcoI C'CATG_G

Cuts at:      0    2757    3216 
   Size:       2757     459
 
NgoMIV G'CCGG_C

Cuts at:      0    1885    3216 
   Size:       1885    1331
 
NspI r_CATG'y

Cuts at:      0    1723    2182    2673    3216 
   Size:       1723     459     491     543

  Fragments arranged by size: 

                1723     543     491     459
 
PasI CC'CwG_GG

Cuts at:      0    1695    3216 
   Size:       1695    1521
 
PciI A'CATG_T

Cuts at:      0    1719    2178    3216 
   Size:       1719     459    1038

  Fragments arranged by size: 

                1719    1038     459
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2287    2319    3216 
   Size:       2287      32     897

  Fragments arranged by size: 

                2287     897      32
 
PfoI T'CCnGG_A

Cuts at:      0     767    2264    2990    3216 
   Size:        767    1497     726     226

  Fragments arranged by size: 

                1497     767     726     226
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     355     387    3216 
   Size:        355      32    2829

  Fragments arranged by size: 

                2829     355      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     495     633    1699    2060    3216 
   Size:        495     138    1066     361    1156

  Fragments arranged by size: 

                1156    1066     495     361     138
 
PsiI TTA'TAA

Cuts at:      0    1033    3216 
   Size:       1033    2183
 
PspOMI G'GGCC_C

Cuts at:      0     279    1423    2573    3216 
   Size:        279    1144    1150     643

  Fragments arranged by size: 

                1150    1144     643     279
 
PstI C_TGCA'G

Cuts at:      0    1551    2330    2468    3216 
   Size:       1551     779     138     748

  Fragments arranged by size: 

                1551     779     748     138
 
PvuII CAG'CTG

Cuts at:      0     197     336    1794    2287    2484    2520    3216 
   Size:        197     139    1458     493     197      36     696

  Fragments arranged by size: 

                1458     696     493     197     197     139      36
 
SacI G_AGCT'C

Cuts at:      0     644     845    1334    3216 
   Size:        644     201     489    1882

  Fragments arranged by size: 

                1882     644     489     201
 
SbfI CC_TGCA'GG

Cuts at:      0    2468    3216 
   Size:       2468     748
 
SexAI A'CCwGG_T

Cuts at:      0     233    3216 
   Size:        233    2983
 
SfcI C'TryA_G

Cuts at:      0    1088    1301    1494    1547    1832    2326    2464    3216 
   Size:       1088     213     193      53     285     494     138     752

  Fragments arranged by size: 

                1088     752     494     285     213     193     138      53
            
 
SfoI GGC'GCC

Cuts at:      0       5    3216 
   Size:          5    3211
 
SmaI CCC'GGG

Cuts at:      0     890    2164    3216 
   Size:        890    1274    1052

  Fragments arranged by size: 

                1274    1052     890
 
SmlI C'TyrA_G

Cuts at:      0    2715    3216 
   Size:       2715     501
 
SrfI GCCC'GGGC

Cuts at:      0    2164    3216 
   Size:       2164    1052
 
SspI AAT'ATT

Cuts at:      0    2019    3216 
   Size:       2019    1197
 
StuI AGG'CCT

Cuts at:      0     327    1066    3216 
   Size:        327     739    2150

  Fragments arranged by size: 

                2150     739     327
 
StyI C'CwwG_G

Cuts at:      0    1143    2434    2757    3216 
   Size:       1143    1291     323     459

  Fragments arranged by size: 

                1291    1143     459     323
 
TatI w'GTAC_w

Cuts at:      0     542     895    1375    1737    2354    2610    2981    3216 
   Size:        542     353     480     362     617     256     371     235

  Fragments arranged by size: 

                 617     542     480     371     362     353     256     235
            
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     201    2121    3216 
   Size:        201    1920    1095

  Fragments arranged by size: 

                1920    1095     201
 
XmaI C'CCGG_G

Cuts at:      0     888    2162    3216 
   Size:        888    1274    1054

  Fragments arranged by size: 

                1274    1054     888
 
ZraI GAC'GTC

Cuts at:      0    1349    3216 
   Size:       1349    1867
 

 Enzymes that do cut:

    AatII     AccI     AcuI     AfeI   AflIII     AhdI     AleI     AloI
    AlwNI     ApaI     ApoI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BlpI Bme1580I    BmgBI     BmrI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI    BsrFI    BsrGI
   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI     ClaI
   DraIII     DrdI     EaeI     EagI     EarI  EcoICRI  Eco57MI EcoO109I
    EcoRV     FalI     FseI    HaeII    Hin4I   HincII     KasI     MfeI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV
     NspI     PasI     PciI    PflMI     PfoI     PpiI    PpuMI     PsiI
   PspOMI     PstI    PvuII     SacI     SbfI    SexAI     SfcI     SfoI
     SmaI     SmlI     SrfI     SspI     StuI     StyI     TatI     XcmI
     XmaI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI    AflII     AgeI     AlfI    ApaLI     AscI
     AseI    AsiSI     BaeI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BmtI    BsaAI    BsaBI    BsaWI    BseYI    BsiWI     BsmI
    BspEI    BspHI    BsrBI   BssHII    BssSI    BstBI   BstEII  BstZ17I
    CspCI     DraI     EciI    EcoNI    EcoRI     FspI    FspAI  HindIII
     HpaI     KpnI     MluI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PmeI     PmlI    PshAI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     SapI     ScaI     SfiI     SgfI    SgrAI
    SnaBI     SpeI     SphI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmnI
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