Restriction map for pF1KSDA0317
##################################
# NO CUT ENZYMES (   89 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AgeI        AhdI        AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AvrII       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BsaBI       BseAI       BsiEI       BsiWI       Bsp1407I    
BspDI       BspEI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      ClaI        CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       FbaI        FseI        HgaI        
HpaI        KpnI        KspI        MamI        MluI        
MroI        NotI        NruI        NspV        PacI        
PinAI       PmeI        PshAI       PshBI       Psp1406I    
PvuI        RsrII       SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   65 enzymes) #
###################################

AccII         112  CGCG                 CG^CG
AcyI         1121  GGCGCC               G[GA]^CG[CT]C
Alw44I       2413  GTGCAC               G^TGCAC
AlwNI          26  CAGTGTCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI        2413  GTGCAC               G^TGCAC
AspI         1177  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         395  TGCGCA               TGC^GCA
BamHI         165  GGATCC               G^GATCC
BbeI         1121  GGCGCC               GGCGC^C
BbrPI          80  CACGTG               CAC^GTG
BbsI         1003  GAAGAC               GAAGAC(2/6)
BlpI         1236  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1236  GCTCAGC              GC^T[ACGT]AGC
BpuAI        1003  GAAGAC               GAAGAC(2/6)
BsaHI        1121  GGCGCC               G[GA]^CG[CT]C
BsaI         1309  GAGACC               GGTCTC(1/5)
BsaWI         128  ACCGGA               [AT]^CCGG[AT]
BsmBI        1421  CGTCTC               CGTCTC(1/5)
BspLU11I     2365  ACATGT               A^CATGT
BsrDI         857  GCAATG               GCAATG(2/0)
BsrFI         971  GCCGGC               [GA]^CCGG[CT]
BssSI        1672  CTCGTG               CACGAG(-5/-1)
BstAPI       2415  GCACAGGATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         112  CGCG                 CG^CG
BstYI         165  GGATCC               [GA]^GATC[CT]
CelII        1236  GCTCAGC              GC^T[ACGT]AGC
Cfr10I        971  GCCGGC               [GA]^CCGG[CT]
DraI         1977  TTTAAA               TTT^AAA
DrdI          814  GACATTATTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI          931  CCATGG               C^C[GA][CT]GG
EcoNI         825  CCTAAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1810  GAATTC               G^AATTC
EcoT22I      1777  ATGCAT               ATGCA^T
FspI          395  TGCGCA               TGC^GCA
Hin1I        1121  GGCGCC               G[GA]^CG[CT]C
HincII        988  GTTGAC               GT[CT]^[GA]AC
HindII        988  GTTGAC               GT[CT]^[GA]AC
HindIII       427  AAGCTT               A^AGCTT
KasI         1121  GGCGCC               G^GCGCC
MfeI         1903  CAATTG               C^AATTG
MflI          165  GGATCC               [GA]^GATC[CT]
MunI         1903  CAATTG               C^AATTG
MvnI          112  CGCG                 CG^CG
NaeI          971  GCCGGC               GCC^GGC
NarI         1121  GGCGCC               GG^CGCC
NcoI          931  CCATGG               C^CATGG
NgoMIV        971  GCCGGC               G^CCGGC
NheI          318  GCTAGC               G^CTAGC
NsiI         1777  ATGCAT               ATGCA^T
PaeR7I        752  CTCGAG               C^TCGAG
PflFI        1177  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         219  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI          80  CACGTG               CAC^GTG
PmlI           80  CACGTG               CAC^GTG
PpuMI         200  AGGACCC              [GA]G^G[AT]CC[CT]
PstI         2425  CTGCAG               CTGCA^G
SapI         1572  GCTCTTC              GCTCTTC(1/4)
SfoI         1121  GGCGCC               GGC^GCC
SphI         2462  GCATGC               GCATG^C
StuI         2043  AGGCCT               AGG^CCT
SwaI         1976  ATTTAAAT             ATTT^AAAT
Tth111I      1177  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        219  CCACAGCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI          752  CTCGAG               C^TCGAG
XhoII         165  GGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AflIII         81  ACGTGT               A^C[GA][CT]GT
             2365  ACATGT              
AlwI          166  GATCC                GGATC(4/5)
              165  GGATC               
AvaI          572  CCCGAG               C^[CT]CG[GA]G
              752  CTCGAG              
BalI         2003  TGGCCA               TGG^CCA
             2432  TGGCCA              
BanI          935  GGCACC               G^G[CT][GA]CC
             1121  GGCGCC              
BsaAI          80  CACGTG               [CT]AC^GT[GA]
              145  TACGTG              
BsgI          272  CTGCAC               GTGCAG(16/14)
             2357  CTGCAC              
BsoBI         572  CCCGAG               C^[CT]CG[GA]G
              752  CTCGAG              
BspMI         938  ACCTGC               ACCTGC(4/8)
             1353  GCAGGT              
DpnII         166  GATC                 ^GATC
             1110  GATC                
DraII         200  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1512  AGGGCCT             
EcoO109I      200  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1512  AGGGCCT             
EcoT14I       931  CCATGG               C^C[AT][AT]GG
             1167  CCTTGG              
MboI          166  GATC                 ^GATC
             1110  GATC                
MluNI        2003  TGGCCA               TGG^CCA
             2432  TGGCCA              
MscI         2003  TGGCCA               TGG^CCA
             2432  TGGCCA              
NdeI         1360  CATATG               CA^TATG
             2393  CATATG              
NdeII         166  GATC                 ^GATC
             1110  GATC                
Sau3AI        166  GATC                 ^GATC
             1110  GATC                
ScaI          673  AGTACT               AGT^ACT
             1787  AGTACT              
SfaNI        2421  GATGC                GCATC(5/9)
             1611  GCATC               
SfcI          918  CTGTAG               C^T[GA][CT]AG
             2425  CTGCAG              
StyI          931  CCATGG               C^C[AT][AT]GG
             1167  CCTTGG              
TfiI           20  GAATC                G^A[AT]TC
             1417  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        ApoI        
Asp700I     AspHI       AvaII       BanII       BbvI        
BcnI        BfaI        BmyI        BpmI        BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    BspHI       BsrBI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
CfoI        Cfr13I      DdeI        EaeI        EarI        
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboII       MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NlaIII      NlaIV       NspI        PleI        
PvuII       RcaI        RsaI        SacI        Sau96I      
ScrFI       SmlI        TaqI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XmnI        
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