Restriction map for pF1KSDA0317
 (Linear) (Six-base) MAPSORT of: KIAA0317.seq  Check: 3766  from: 1  to: 2469



KIAA0317.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     947    2469 
   Size:        947    1522
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     854    1126    1411    2239    2469 
   Size:        337     517     272     285     828     230

  Fragments arranged by size: 

                 828     517     337     285     272     230
 
AflIII A'CryG_T

Cuts at:      0      81    2365    2469 
   Size:         81    2284     104

  Fragments arranged by size: 

                2284     104      81
 
AleI CACnn'nnGTG

Cuts at:      0    1201    2469 
   Size:       1201    1268
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     281     313     473     505    2469 
   Size:        281      32     160      32    1964

  Fragments arranged by size: 

                1964     281     160      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      31    2469 
   Size:         31    2438
 
ApaLI G'TGCA_C

Cuts at:      0    2413    2469 
   Size:       2413      56
 
ApoI r'AATT_y

Cuts at:      0     329     467     808    1156    1440    1810    1980    2469 
   Size:        329     138     341     348     284     370     170     489

  Fragments arranged by size: 

                 489     370     348     341     329     284     170     138
            
 
AvaI C'yCGr_G

Cuts at:      0     572     752    2469 
   Size:        572     180    1717

  Fragments arranged by size: 

                1717     572     180
 
BamHI G'GATC_C

Cuts at:      0     165    2469 
   Size:        165    2304
 
BanI G'GyrC_C

Cuts at:      0     935    1121    2469 
   Size:        935     186    1348

  Fragments arranged by size: 

                1348     935     186
 
BanII G_rGCy'C

Cuts at:      0     362     653    1238    1734    1941    2469 
   Size:        362     291     585     496     207     528

  Fragments arranged by size: 

                 585     528     496     362     291     207
 
BbeI G_GCGC'C

Cuts at:      0    1125    2469 
   Size:       1125    1344
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1010    2469 
   Size:       1010    1459
 
BfrBI ATG'CAT

Cuts at:      0    1779    2469 
   Size:       1779     690
 
BlpI GC'TnA_GC

Cuts at:      0    1237    2469 
   Size:       1237    1232
 
Bme1580I G_kGCm'C

Cuts at:      0    2417    2469 
   Size:       2417      52
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1515    2469 
   Size:       1515     954
 
BmtI G_CTAG'C

Cuts at:      0     322    2469 
   Size:        322    2147
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1931    2141    2309    2469 
   Size:         82    1849     210     168     160

  Fragments arranged by size: 

                1849     210     168     160      82
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     633     644    2119    2469 
   Size:        633      11    1475     350

  Fragments arranged by size: 

                1475     633     350      11
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1303    2469 
   Size:       1303    1166
 
BsaAI yAC'GTr

Cuts at:      0      82     147    2469 
   Size:         82      65    2322

  Fragments arranged by size: 

                2322      82      65
 
BsaHI Gr'CG_yC

Cuts at:      0    1122    2469 
   Size:       1122    1347
 
BsaWI w'CCGG_w

Cuts at:      0     128    2469 
   Size:        128    2341
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1721    2035    2251    2262    2469 
   Size:       1721     314     216      11     207

  Fragments arranged by size: 

                1721     314     216     207      11
 
BseYI C'CCAG_C

Cuts at:      0     403     965    2469 
   Size:        403     562    1504

  Fragments arranged by size: 

                1504     562     403
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     257    2342    2469 
   Size:        257    2085     127

  Fragments arranged by size: 

                2085     257     127
 
BsiHKAI G_wGCw'C

Cuts at:      0     626     653    1238    1734    1941    2417    2469 
   Size:        626      27     585     496     207     476      52

  Fragments arranged by size: 

                 626     585     496     476     207      52      27
 
BsmI GAATG_Cn'

Cuts at:      0      40     747    1217    2469 
   Size:         40     707     470    1252

  Fragments arranged by size: 

                1252     707     470      40
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1427    2469 
   Size:       1427    1042
 
Bsp1286I G_dGCh'C

Cuts at:      0     362     626     653    1238    1734    1941    2417    2469 
   Size:        362     264      27     585     496     207     476      52

  Fragments arranged by size: 

                 585     496     476     362     264     207      52      27
            
 
BspHI T'CATG_A

Cuts at:      0     645    1925    2195    2469 
   Size:        645    1280     270     274

  Fragments arranged by size: 

                1280     645     274     270
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     947    1344    2469 
   Size:        947     397    1125

  Fragments arranged by size: 

                1125     947     397
 
BsrBI CCG'CTC

Cuts at:      0    1280    1633    1745    2332    2469 
   Size:       1280     353     112     587     137

  Fragments arranged by size: 

                1280     587     353     137     112
 
BsrDI GCAATG_nn'

Cuts at:      0     864    2469 
   Size:        864    1605
 
BsrFI r'CCGG_y

Cuts at:      0     971    2469 
   Size:        971    1498
 
BssSI C'ACGA_G

Cuts at:      0    1672    2469 
   Size:       1672     797
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    2421    2469 
   Size:       2421      48
 
BstYI r'GATC_y

Cuts at:      0     165    2469 
   Size:        165    2304
 
BtgI C'CryG_G

Cuts at:      0     931    2469 
   Size:        931    1538
 
BtsI GCAGTG_nn'

Cuts at:      0     329     980    2469 
   Size:        329     651    1489

  Fragments arranged by size: 

                1489     651     329
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     969    1004    2469 
   Size:        969      35    1465

  Fragments arranged by size: 

                1465     969      35
 
DraI TTT'AAA

Cuts at:      0    1979    2469 
   Size:       1979     490
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     820    2469 
   Size:        820    1649
 
EaeI y'GGCC_r

Cuts at:      0     973    2003    2432    2469 
   Size:        973    1030     429      37

  Fragments arranged by size: 

                1030     973     429      37
 
EarI CTCTTCn'nnn_

Cuts at:      0     662     966    1579    1874    2402    2469 
   Size:        662     304     613     295     528      67

  Fragments arranged by size: 

                 662     613     528     304     295      67
 
EcoICRI GAG'CTC

Cuts at:      0     651    1236    1732    1939    2469 
   Size:        651     585     496     207     530

  Fragments arranged by size: 

                 651     585     530     496     207
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82     337     854    1126    1411    1931    2141    2239 
   Size:         82     255     517     272     285     520     210      98

Cuts at:   2239    2309    2469 
   Size:         70     160

  Fragments arranged by size: 

                 520     517     285     272     255     210     160      98
                  82      70
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     829    2469 
   Size:        829    1640
 
EcoO109I rG'GnC_Cy

Cuts at:      0     201    1513    2469 
   Size:        201    1312     956

  Fragments arranged by size: 

                1312     956     201
 
EcoRI G'AATT_C

Cuts at:      0    1810    2469 
   Size:       1810     659
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      81    1347    2469 
   Size:         81    1266    1122

  Fragments arranged by size: 

                1266    1122      81
 
FspI TGC'GCA

Cuts at:      0     397    2469 
   Size:        397    2072
 
HaeII r_GCGC'y

Cuts at:      0     120     972    1125    2469 
   Size:        120     852     153    1344

  Fragments arranged by size: 

                1344     852     153     120
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     157     189     287     319     991     997    1023    1029 
   Size:        157      32      98      32     672       6      26       6

Cuts at:   1029    2177    2209    2469 
   Size:       1148      32     260

  Fragments arranged by size: 

                1148     672     260     157      98      32      32      32
                  26       6       6
 
HincII GTy'rAC

Cuts at:      0     990    2469 
   Size:        990    1479
 
HindIII A'AGCT_T

Cuts at:      0     427    2469 
   Size:        427    2042
 
KasI G'GCGC_C

Cuts at:      0    1121    2469 
   Size:       1121    1348
 
MfeI C'AATT_G

Cuts at:      0    1903    2469 
   Size:       1903     566
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     731    1394    2359    2469 
   Size:        392     339     663     965     110

  Fragments arranged by size: 

                 965     663     392     339     110
 
MscI TGG'CCA

Cuts at:      0    2005    2434    2469 
   Size:       2005     429      35

  Fragments arranged by size: 

                2005     429      35
 
MslI CAynn'nnrTG

Cuts at:      0     952    1201    2131    2364    2469 
   Size:        952     249     930     233     105

  Fragments arranged by size: 

                 952     930     249     233     105
 
MspA1I CmG'CkG

Cuts at:      0    1241    2379    2430    2469 
   Size:       1241    1138      51      39

  Fragments arranged by size: 

                1241    1138      51      39
 
NaeI GCC'GGC

Cuts at:      0     973    2469 
   Size:        973    1496
 
NarI GG'CG_CC

Cuts at:      0    1122    2469 
   Size:       1122    1347
 
NcoI C'CATG_G

Cuts at:      0     931    2469 
   Size:        931    1538
 
NdeI CA'TA_TG

Cuts at:      0    1361    2394    2469 
   Size:       1361    1033      75

  Fragments arranged by size: 

                1361    1033      75
 
NgoMIV G'CCGG_C

Cuts at:      0     971    2469 
   Size:        971    1498
 
NheI G'CTAG_C

Cuts at:      0     318    2469 
   Size:        318    2151
 
NsiI A_TGCA'T

Cuts at:      0    1781    2469 
   Size:       1781     688
 
NspI r_CATG'y

Cuts at:      0    1408    2369    2466    2469 
   Size:       1408     961      97       3

  Fragments arranged by size: 

                1408     961      97       3
 
PciI A'CATG_T

Cuts at:      0    2365    2469 
   Size:       2365     104
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     225    2469 
   Size:        225    2244
 
PfoI T'CCnGG_A

Cuts at:      0    1146    1484    2469 
   Size:       1146     338     985

  Fragments arranged by size: 

                1146     985     338
 
PmlI CAC'GTG

Cuts at:      0      82    2469 
   Size:         82    2387
 
PpuMI rG'GwC_Cy

Cuts at:      0     201    2469 
   Size:        201    2268
 
PsiI TTA'TAA

Cuts at:      0     905    2469 
   Size:        905    1564
 
PstI C_TGCA'G

Cuts at:      0    2429    2469 
   Size:       2429      40
 
PvuII CAG'CTG

Cuts at:      0    1241    2379    2430    2469 
   Size:       1241    1138      51      39

  Fragments arranged by size: 

                1241    1138      51      39
 
SacI G_AGCT'C

Cuts at:      0     653    1238    1734    1941    2469 
   Size:        653     585     496     207     528

  Fragments arranged by size: 

                 653     585     528     496     207
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1579    2469 
   Size:       1579     890
 
ScaI AGT'ACT

Cuts at:      0     675    1789    2469 
   Size:        675    1114     680

  Fragments arranged by size: 

                1114     680     675
 
SfcI C'TryA_G

Cuts at:      0     918    2425    2469 
   Size:        918    1507      44

  Fragments arranged by size: 

                1507     918      44
 
SfoI GGC'GCC

Cuts at:      0    1123    2469 
   Size:       1123    1346
 
SmlI C'TyrA_G

Cuts at:      0     612     659     752    2098    2469 
   Size:        612      47      93    1346     371

  Fragments arranged by size: 

                1346     612     371      93      47
 
SphI G_CATG'C

Cuts at:      0    2466    2469 
   Size:       2466       3
 
StuI AGG'CCT

Cuts at:      0    2045    2469 
   Size:       2045     424
 
StyI C'CwwG_G

Cuts at:      0     931    1167    2469 
   Size:        931     236    1302

  Fragments arranged by size: 

                1302     931     236
 
SwaI ATTT'AAAT

Cuts at:      0    1979    2469 
   Size:       1979     490
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     960    2469 
   Size:        960    1509
 
TatI w'GTAC_w

Cuts at:      0     673    1058    1787    2469 
   Size:        673     385     729     682

  Fragments arranged by size: 

                 729     682     673     385
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      11      43     428     460    1658    1690    2469 
   Size:         11      32     385      32    1198      32     779

  Fragments arranged by size: 

                1198     779     385      32      32      32      11
 
Tth111I GACn'n_nGTC

Cuts at:      0    1180    2469 
   Size:       1180    1289
 
XhoI C'TCGA_G

Cuts at:      0     752    2469 
   Size:        752    1717
 
XmnI GAAnn'nnTTC

Cuts at:      0     350    1096    1333    2469 
   Size:        350     746     237    1136

  Fragments arranged by size: 

                1136     746     350     237
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AleI     AloI    AlwNI    ApaLI     ApoI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BfrBI     BlpI
 Bme1580I     BmrI     BmtI     BpmI    BpuEI     BsaI    BsaAI    BsaHI
    BsaWI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstYI
     BtgI     BtsI    CspCI     DraI     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     MfeI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NsiI     NspI
     PciI    PflMI     PfoI     PmlI    PpuMI     PsiI     PstI    PvuII
     SacI     SapI     ScaI     SfcI     SfoI     SmlI     SphI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XhoI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     ApaI     AscI     AseI    AsiSI    AvrII     BaeI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII    BmgBI     BplI   Bpu10I
    BsaBI    BsaXI    BsiEI    BsiWI    BspEI    BsrGI   BssHII    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI   DraIII     EagI
     EciI    EcoRV     FseI    FspAI     HpaI     KpnI     MluI     NotI
     NruI     PacI     PasI     PmeI     PpiI    PshAI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI    SexAI
     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI
     XbaI     XcmI     XmaI     ZraI
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