Restriction map for pF1KSDA0313
##################################
# NO CUT ENZYMES (   82 enzymes) #
##################################

AccIII      AclI        AflII       AflIII      AgeI        
Alw44I      Aor51HI     ApaI        ApaLI       AscI        
AseI        AsnI        AspHI       AviII       BbrPI       
BbvCI       BcgI        BciVI       BfrI        BsaAI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstBI       BstZ17I     Cfr10I      ClaI        CpoI        
EagI        EclXI       Eco47III    Eco52I      FseI        
FspI        KspI        MluI        MroI        NaeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SexAI       
SfiI        SfuI        SgrAI       SmaI        SnaBI       
SphI        SspBI       SwaI        XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AatII        3747  GACGTC               GACGT^C
Acc65I        820  GGTACC               G^GTACC
AccI         2662  GTAGAC               GT^[AC][GT]AC
Asp718I       820  GGTACC               G^GTACC
AspI          417  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII        3549  CCTAGG               C^CTAGG
BalI         1311  TGGCCA               TGG^CCA
BamHI        3849  GGATCC               G^GATCC
BbeI         3512  GGCGCC               GGCGC^C
BlnI         3549  CCTAGG               C^CTAGG
BlpI         2145  GCTCAGC              GC^T[ACGT]AGC
BpmI         3065  CTCCAG               CTGGAG(16/14)
Bpu1102I     2145  GCTCAGC              GC^T[ACGT]AGC
BsaWI         350  ACCGGA               [AT]^CCGG[AT]
BspHI        1865  TCATGA               T^CATGA
BstAPI        390  GCACCAGTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        111  GGTGACC              G^GT[ACGT]ACC
BstPI         111  GGTGACC              G^GT[ACGT]ACC
CelII        2145  GCTCAGC              GC^T[ACGT]AGC
DraIII       1952  CACTATGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2665  GACGGGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I       111  GGTGACC              G^GT[ACGT]ACC
EcoRI        4223  GAATTC               G^AATTC
EcoRV         181  GATATC               GAT^ATC
HaeII        3512  GGCGCC               [GA]GCGC^[CT]
KasI         3512  GGCGCC               G^GCGCC
KpnI          820  GGTACC               GGTAC^C
MfeI          344  CAATTG               C^AATTG
MluNI        1311  TGGCCA               TGG^CCA
MscI         1311  TGGCCA               TGG^CCA
MunI          344  CAATTG               C^AATTG
NarI         3512  GGCGCC               GG^CGCC
NdeI         3880  CATATG               CA^TATG
NspI          722  ACATGC               [GA]CATG^[CT]
PflFI         417  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3220  CCACCTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI         1865  TCATGA               T^CATGA
SbfI         3853  CCTGCAGG             CCTGCA^GG
ScaI         4147  AGTACT               AGT^ACT
SfoI         3512  GGCGCC               GGC^GCC
SpeI          151  ACTAGT               A^CTAGT
Sse8387I     3853  CCTGCAGG             CCTGCA^GG
SspI         1946  AATATT               AAT^ATT
StuI         4381  AGGCCT               AGG^CCT
Tth111I       417  GACAATGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3220  CCACCTTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         3398  CCAGTGGTACTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwNI        3103  CAGTCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             3422  CAGATTCTG           
BanII        4159  GGGCTC               G[GA]GC[CT]^C
             4192  GAGCCC              
BcnI         1938  CCCGG                CC^[GC]GG
             4210  CCCGG               
BglI         2911  GCCCAAATGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             4356  GCCACATGGGC         
BglII        1509  AGATCT               A^GATCT
             2514  AGATCT              
BsaBI        1623  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2352  GATGAAGATC          
BsgI         2386  CTGCAC               GTGCAG(16/14)
             3499  CTGCAC              
BspMI        3856  GCAGGT               ACCTGC(4/8)
             4181  GCAGGT              
BstXI        1571  CCAACACTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2540  CCAGAAACATGG        
DraII         338  AGGACCC              [GA]G^G[ACGT]CC[CT]
             3607  GGGTCCT             
EcoNI         330  CCTAGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              824  CCTCAGAAAGG         
EcoO109I      338  AGGACCC              [GA]G^G[ACGT]CC[CT]
             3607  GGGTCCT             
EcoT22I       841  ATGCAT               ATGCA^T
             3326  ATGCAT              
HgaI         3709  GCGTC                GACGC(5/10)
             3979  GCGTC               
HindIII      2896  AAGCTT               A^AGCTT
             3245  AAGCTT              
HpaI          834  GTTAAC               GTT^AAC
             1161  GTTAAC              
MamI         1623  GATCAGTATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             2352  GATGAAGATC          
MslI         1018  CACTTCAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             4131  CATAGCTGTG          
NciI         1938  CCCGG                CC^[GC]GG
             4210  CCCGG               
NcoI           27  CCATGG               C^CATGG
              611  CCATGG              
NsiI          841  ATGCAT               ATGCA^T
             3326  ATGCAT              
PpuMI         338  AGGACCC              [GA]G^G[AT]CC[CT]
             3607  GGGTCCT             
SapI          502  GAAGAGC              GCTCTTC(1/4)
              853  GAAGAGC             
SmlI         1673  CTCAAG               C^T[CT][GA]AG
             3756  CTCAAG              


#########################################
# 3 OR MORE CUT ENZYMES (  105 enzymes) #
#########################################

AccII       AciI        AcsI        AcyI        AfaI        
AhdI        AluI        AlwI        AocI        ApoI        
Asp700I     AspEI       AvaI        AvaII       BanI        
BbsI        BbvI        BclI        BfaI        BmyI        
BpuAI       BsaHI       BsaI        BsaJI       BseRI       
BsiYI       BslI        BsmAI       BsmFI       BsmI        
BsoBI       Bsp1286I    BsrDI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Bsu36I      
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraI        DsaI        EaeI        Eam1105I    EarI        
Eco81I      EcoRII      EcoT14I     FauI        FbaI        
Fnu4HI      FokI        HaeIII      HapII       HhaI        
Hin1I       HinP1I      HincII      HindII      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MspA1I      MspI        MvaI        
MvnI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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