Restriction map for pF1KSDA0290
 (Linear) (Six-base) MAPSORT of: KIAA0290.seq  Check: 9944  from: 1  to: 2667



KIAA0290.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     681     918    1278    1650    2667 
   Size:        681     237     360     372    1017

  Fragments arranged by size: 

                1017     681     372     360     237
 
AclI AA'CG_TT

Cuts at:      0     843    2667 
   Size:        843    1824
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1757    2667 
   Size:       1757     910
 
AflIII A'CryG_T

Cuts at:      0     946    1720    2667 
   Size:        946     774     947

  Fragments arranged by size: 

                 947     946     774
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1437    1828    2667 
   Size:       1437     391     839

  Fragments arranged by size: 

                1437     839     391
 
AleI CACnn'nnGTG

Cuts at:      0     676    2338    2667 
   Size:        676    1662     329

  Fragments arranged by size: 

                1662     676     329
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2282    2432    2667 
   Size:       2282     150     235

  Fragments arranged by size: 

                2282     235     150
 
AlwNI CAG_nnn'CTG

Cuts at:      0     399    1259    2370    2667 
   Size:        399     860    1111     297

  Fragments arranged by size: 

                1111     860     399     297
 
ApaI G_GGCC'C

Cuts at:      0      80    2525    2667 
   Size:         80    2445     142

  Fragments arranged by size: 

                2445     142      80
 
ApaLI G'TGCA_C

Cuts at:      0    1075    2667 
   Size:       1075    1592
 
ApoI r'AATT_y

Cuts at:      0     703    2667 
   Size:        703    1964
 
AvaI C'yCGr_G

Cuts at:      0     381     554    1097    1117    1835    1946    2235    2667 
   Size:        381     173     543      20     718     111     289     432

  Fragments arranged by size: 

                 718     543     432     381     289     173     111      20
            
 
BanI G'GyrC_C

Cuts at:      0    1183    1449    1489    1518    1548    2329    2667 
   Size:       1183     266      40      29      30     781     338

  Fragments arranged by size: 

                1183     781     338     266      40      30      29
 
BanII G_rGCy'C

Cuts at:      0      80     649    1016    1061    1104    1504    1756    1771 
   Size:         80     569     367      45      43     400     252      15

Cuts at:   1771    1849    1864    2525    2667 
   Size:         78      15     661     142

  Fragments arranged by size: 

                 661     569     400     367     252     142      80      78
                  45      43      15      15
 
BbeI G_GCGC'C

Cuts at:      0    1522    2667 
   Size:       1522    1145
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     210    2667 
   Size:        210    2457
 
BbvCI CC'TCA_GC

Cuts at:      0    2026    2667 
   Size:       2026     641
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1552    2667 
   Size:       1552    1115
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1181    2667 
   Size:       1181    1486
 
Bme1580I G_kGCm'C

Cuts at:      0      80     772    1079    1186    1551    2271    2345    2525 
   Size:         80     692     307     107     365     720      74     180

Cuts at:   2525    2667 
   Size:        142

  Fragments arranged by size: 

                 720     692     365     307     180     142     107      80
                  74
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1774    1964    2238    2667 
   Size:       1774     190     274     429

  Fragments arranged by size: 

                1774     429     274     190
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     156    2308    2532    2667 
   Size:        156    2152     224     135

  Fragments arranged by size: 

                2152     224     156     135
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1088    1339    1359    1574    1627    2452    2667 
   Size:        949     139     251      20     215      53     825     215

  Fragments arranged by size: 

                 949     825     251     215     215     139      53      20
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1138    1750    2026    2667 
   Size:       1138     612     276     641

  Fragments arranged by size: 

                1138     641     612     276
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304    2667 
   Size:        304    2363
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     127    1224    1619    2110    2307    2667 
   Size:        127    1097     395     491     197     360

  Fragments arranged by size: 

                1097     491     395     360     197     127
 
BsaAI yAC'GTr

Cuts at:      0     678    1723    2667 
   Size:        678    1045     944

  Fragments arranged by size: 

                1045     944     678
 
BsaHI Gr'CG_yC

Cuts at:      0     386    1519    2667 
   Size:        386    1133    1148

  Fragments arranged by size: 

                1148    1133     386
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     447     477    1276    1306    1816    1846    2212    2242 
   Size:        447      30     799      30     510      30     366      30

Cuts at:   2242    2667 
   Size:        425

  Fragments arranged by size: 

                 799     510     447     425     366      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145     219     625    1297    1383    1386    1637    2449 
   Size:        145      74     406     672      86       3     251     812

Cuts at:   2449    2646    2667 
   Size:        197      21

  Fragments arranged by size: 

                 812     672     406     251     197     145      86      74
                  21       3
 
BseYI C'CCAG_C

Cuts at:      0     642    1216    1507    1801    1933    2504    2524    2533 
   Size:        642     574     291     294     132     571      20       9

Cuts at:   2533    2667 
   Size:        134

  Fragments arranged by size: 

                 642     574     571     294     291     134     132      20
                   9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     391     700    1283    1622    2531    2667 
   Size:        391     309     583     339     909     136

  Fragments arranged by size: 

                 909     583     391     339     309     136
 
BsiEI CG_ry'CG

Cuts at:      0    1010    2667 
   Size:       1010    1657
 
BsiHKAI G_wGCw'C

Cuts at:      0     358    1079    2063    2667 
   Size:        358     721     984     604

  Fragments arranged by size: 

                 984     721     604     358
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     231    1592    2667 
   Size:        231    1361    1075

  Fragments arranged by size: 

                1361    1075     231
 
Bsp1286I G_dGCh'C

Cuts at:      0      80     358     649     772    1016    1061    1079    1104 
   Size:         80     278     291     123     244      45      18      25

Cuts at:   1104    1186    1504    1551    1756    1771    1849    1864    2063 
   Size:         82     318      47     205      15      78      15     199

Cuts at:   2063    2271    2345    2525    2667 
   Size:        208      74     180     142

  Fragments arranged by size: 

                 318     291     278     244     208     205     199     180
                 142     123      82      80      78      74      47      45
                  25      18      15      15
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     363     681     918    1278    1650    2667 
   Size:        363     318     237     360     372    1017

  Fragments arranged by size: 

                1017     372     363     360     318     237
 
BsrBI CCG'CTC

Cuts at:      0     207     447     894    2514    2667 
   Size:        207     240     447    1620     153

  Fragments arranged by size: 

                1620     447     240     207     153
 
BsrDI GCAATG_nn'

Cuts at:      0    1734    2667 
   Size:       1734     933
 
BsrFI r'CCGG_y

Cuts at:      0     884    2321    2412    2667 
   Size:        884    1437      91     255

  Fragments arranged by size: 

                1437     884     255      91
 
BsrGI T'GTAC_A

Cuts at:      0    2040    2667 
   Size:       2040     627
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     576     814    2667 
   Size:        576     238    1853

  Fragments arranged by size: 

                1853     576     238
 
BstEII G'GTnAC_C

Cuts at:      0    2608    2667 
   Size:       2608      59
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      96    1472    1598    1941    2541    2667 
   Size:         96    1376     126     343     600     126

  Fragments arranged by size: 

                1376     600     343     126     126      96
 
BstYI r'GATC_y

Cuts at:      0     279    2366    2667 
   Size:        279    2087     301

  Fragments arranged by size: 

                2087     301     279
 
Bsu36I CC'TnA_GG

Cuts at:      0     622    2667 
   Size:        622    2045
 
BtgI C'CryG_G

Cuts at:      0     188     605    1148    1920    2333    2540    2667 
   Size:        188     417     543     772     413     207     127

  Fragments arranged by size: 

                 772     543     417     413     207     188     127
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     163     850    2667 
   Size:        163     687    1817

  Fragments arranged by size: 

                1817     687     163
 
BtsI GCAGTG_nn'

Cuts at:      0     637    1784    2186    2284    2667 
   Size:        637    1147     402      98     383

  Fragments arranged by size: 

                1147     637     402     383      98
 
DraIII CAC_nnn'GTG

Cuts at:      0      66     663    2667 
   Size:         66     597    2004

  Fragments arranged by size: 

                2004     597      66
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2574    2667 
   Size:       2574      93
 
EaeI y'GGCC_r

Cuts at:      0     170    1007    1616    1923    2487    2667 
   Size:        170     837     609     307     564     180

  Fragments arranged by size: 

                 837     609     564     307     180     170
 
EagI C'GGCC_G

Cuts at:      0    1007    2667 
   Size:       1007    1660
 
EarI CTCTTCn'nnn_

Cuts at:      0    1049    1776    2623    2667 
   Size:       1049     727     847      44

  Fragments arranged by size: 

                1049     847     727      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     115     505    2667 
   Size:        115     390    2162

  Fragments arranged by size: 

                2162     390     115
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1088    1339    1359    1574    1627    1757    2452 
   Size:        949     139     251      20     215      53     130     695

Cuts at:   2452    2667 
   Size:        215

  Fragments arranged by size: 

                 949     695     251     215     215     139     130      53
                  20
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     938    1175    2296    2667 
   Size:        938     237    1121     371

  Fragments arranged by size: 

                1121     938     371     237
 
EcoO109I rG'GnC_Cy

Cuts at:      0      51      76     182     194    1476    1594    1665    1734 
   Size:         51      25     106      12    1282     118      71      69

Cuts at:   1734    2006    2239    2521    2667 
   Size:        272     233     282     146

  Fragments arranged by size: 

                1282     282     272     233     146     118     106      71
                  69      51      25      12
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1320    2667 
   Size:       1320    1347
 
HaeII r_GCGC'y

Cuts at:      0     247     531    1522    2169    2667 
   Size:        247     284     991     647     498

  Fragments arranged by size: 

                 991     647     498     284     247
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1276    1308    1423    1455    1814    1846    2667 
   Size:       1276      32     115      32     359      32     821

  Fragments arranged by size: 

                1276     821     359     115      32      32      32
 
KasI G'GCGC_C

Cuts at:      0    1518    2667 
   Size:       1518    1149
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1064    2667 
   Size:       1064    1603
 
MscI TGG'CCA

Cuts at:      0     172    1925    2667 
   Size:        172    1753     742

  Fragments arranged by size: 

                1753     742     172
 
MslI CAynn'nnrTG

Cuts at:      0     676    2338    2647    2667 
   Size:        676    1662     309      20

  Fragments arranged by size: 

                1662     676     309      20
 
MspA1I CmG'CkG

Cuts at:      0     916    1129    1150    1259    1511    1937    2003    2504 
   Size:        916     213      21     109     252     426      66     501

Cuts at:   2504    2606    2667 
   Size:        102      61

  Fragments arranged by size: 

                 916     501     426     252     213     109     102      66
                  61      21
 
NaeI GCC'GGC

Cuts at:      0     886    2414    2667 
   Size:        886    1528     253

  Fragments arranged by size: 

                1528     886     253
 
NarI GG'CG_CC

Cuts at:      0    1519    2667 
   Size:       1519    1148
 
NcoI C'CATG_G

Cuts at:      0     188     605    2667 
   Size:        188     417    2062

  Fragments arranged by size: 

                2062     417     188
 
NdeI CA'TA_TG

Cuts at:      0     651    2667 
   Size:        651    2016
 
NgoMIV G'CCGG_C

Cuts at:      0     884    2412    2667 
   Size:        884    1528     255

  Fragments arranged by size: 

                1528     884     255
 
NspI r_CATG'y

Cuts at:      0     950    2619    2667 
   Size:        950    1669      48

  Fragments arranged by size: 

                1669     950      48
 
PasI CC'CwG_GG

Cuts at:      0    1177    1598    2667 
   Size:       1177     421    1069

  Fragments arranged by size: 

                1177    1069     421
 
PciI A'CATG_T

Cuts at:      0     946    2667 
   Size:        946    1721
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     606    2667 
   Size:        606    2061
 
PfoI T'CCnGG_A

Cuts at:      0     110     873     926    2667 
   Size:        110     763      53    1741

  Fragments arranged by size: 

                1741     763     110      53
 
PmlI CAC'GTG

Cuts at:      0     678    2667 
   Size:        678    1989
 
PpuMI rG'GwC_Cy

Cuts at:      0      51     182     194    1476    1734    2006    2239    2667 
   Size:         51     131      12    1282     258     272     233     428

  Fragments arranged by size: 

                1282     428     272     258     233     131      51      12
            
 
PshAI GACnn'nnGTC

Cuts at:      0    1236    2398    2667 
   Size:       1236    1162     269

  Fragments arranged by size: 

                1236    1162     269
 
PspOMI G'GGCC_C

Cuts at:      0      76    2521    2667 
   Size:         76    2445     146

  Fragments arranged by size: 

                2445     146      76
 
PspXI vC'TCGA_Gb

Cuts at:      0    1946    2667 
   Size:       1946     721
 
PstI C_TGCA'G

Cuts at:      0     830     915    1114    2165    2261    2667 
   Size:        830      85     199    1051      96     406

  Fragments arranged by size: 

                1051     830     406     199      96      85
 
PvuII CAG'CTG

Cuts at:      0     916    1259    1511    1937    2504    2667 
   Size:        916     343     252     426     567     163

  Fragments arranged by size: 

                 916     567     426     343     252     163
 
SacII CC_GC'GG

Cuts at:      0    1151    2667 
   Size:       1151    1516
 
SanDI GG'GwC_CC

Cuts at:      0     182    1476    2006    2239    2667 
   Size:        182    1294     530     233     428

  Fragments arranged by size: 

                1294     530     428     233     182
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1049    1776    2667 
   Size:       1049     727     891

  Fragments arranged by size: 

                1049     891     727
 
SbfI CC_TGCA'GG

Cuts at:      0     830    2667 
   Size:        830    1837
 
SexAI A'CCwGG_T

Cuts at:      0    2654    2667 
   Size:       2654      13
 
SfcI C'TryA_G

Cuts at:      0     826     911     917    1110    1512    2161    2257    2667 
   Size:        826      85       6     193     402     649      96     410

  Fragments arranged by size: 

                 826     649     410     402     193      96      85       6
            
 
SfoI GGC'GCC

Cuts at:      0    1520    2667 
   Size:       1520    1147
 
SmaI CCC'GGG

Cuts at:      0    1099    1837    2237    2667 
   Size:       1099     738     400     430

  Fragments arranged by size: 

                1099     738     430     400
 
SmlI C'TyrA_G

Cuts at:      0     319    1946    2667 
   Size:        319    1627     721

  Fragments arranged by size: 

                1627     721     319
 
SrfI GCCC'GGGC

Cuts at:      0    1099    2667 
   Size:       1099    1568
 
StuI AGG'CCT

Cuts at:      0     862    1612    2667 
   Size:        862     750    1055

  Fragments arranged by size: 

                1055     862     750
 
StyI C'CwwG_G

Cuts at:      0      89     188     359     605     857    1613    1790    2667 
   Size:         89      99     171     246     252     756     177     877

  Fragments arranged by size: 

                 877     756     252     246     177     171      99      89
            
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1226    1695    2167    2311    2547    2667 
   Size:       1226     469     472     144     236     120

  Fragments arranged by size: 

                1226     472     469     236     144     120
 
TatI w'GTAC_w

Cuts at:      0    2040    2667 
   Size:       2040     627
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1816    1848    2006    2038    2667 
   Size:       1816      32     158      32     629

  Fragments arranged by size: 

                1816     629     158      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      93    1450    1665    1788    2278    2427    2667 
   Size:         93    1357     215     123     490     149     240

  Fragments arranged by size: 

                1357     490     240     215     149     123      93
 
XhoI C'TCGA_G

Cuts at:      0    1946    2667 
   Size:       1946     721
 
XmaI C'CCGG_G

Cuts at:      0    1097    1835    2235    2667 
   Size:       1097     738     400     432

  Fragments arranged by size: 

                1097     738     432     400
 

 Enzymes that do cut:

     AarI     AclI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AvaI     BanI    BanII     BbeI     BbsI
    BbvCI    BciVI     BglI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BstAPI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I     FalI    HaeII    Hin4I     KasI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NspI
     PasI     PciI    PflMI     PfoI     PmlI    PpuMI    PshAI   PspOMI
    PspXI     PstI    PvuII    SacII    SanDI     SapI     SbfI    SexAI
     SfcI     SfoI     SmaI     SmlI     SrfI     StuI     StyI    TaqII
     TatI     TstI     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI    AflII     AgeI     AloI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BcgI     BclI    BfrBI
    BglII     BlpI    BmgBI     BmtI    BsaBI    BsaWI    BsiWI     BsmI
    BspEI    BspHI   BssHII    BssSI    BstBI  BstZ17I     ClaI    CspCI
     DraI  EcoICRI    EcoRI    EcoRV     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     NheI     NotI     NruI
     NsiI     PacI     PmeI     PpiI     PsiI     PsrI     PvuI    RsrII
     SacI     SalI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SspI     SwaI  Tth111I     XbaI     XmnI     ZraI
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