Restriction map for pF1KSDA0282
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AflIII      AgeI        AocI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspI        AvrII       BalI        BamHI       
BbrPI       BbvCI       BcgI        BclI        BfrI        
BlnI        BsaAI       BsaBI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspEI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
Bst1107I    BstBI       BstEII      BstPI       BstXI       
BstZ17I     Bsu36I      CpoI        DraI        DraIII      
EagI        EclXI       Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HpaI        KpnI        MamI        MluI        MluNI       
MroI        MscI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SalI        
SapI        SbfI        SexAI       SfiI        SfuI        
SgrAI       SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        Tth111I     XbaI        
XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AhdI         1086  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI      1369  AGCGCT               AGC^GCT
ApaI          604  GGGCCC               GGGCC^C
AspEI        1086  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         893  TGCGCA               TGC^GCA
BbeI          519  GGCGCC               GGCGC^C
BciVI        1420  GGATAC               GTATCC(6/5)
BglI          597  GCCCCGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        1055  AGATCT               A^GATCT
BlpI          660  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      660  GCTGAGC              GC^T[ACGT]AGC
BsaWI        1868  ACCGGA               [AT]^CCGG[AT]
BspDI         970  ATCGAT               AT^CGAT
BssSI         694  CACGAG               CACGAG(-5/-1)
BstAPI        713  GCATGTACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         660  GCTGAGC              GC^T[ACGT]AGC
ClaI          970  ATCGAT               AT^CGAT
DrdI          120  GACCCCAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI          326  CGGCCA               [CT]^GGCC[GA]
Eam1105I     1086  GACCACAGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III     1369  AGCGCT               AGC^GCT
EcoNI        1182  CCTGACTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI          893  TGCGCA               TGC^GCA
HincII       1012  GTCAAC               GT[CT]^[GA]AC
HindII       1012  GTCAAC               GT[CT]^[GA]AC
KasI          519  GGCGCC               G^GCGCC
MfeI         1937  CAATTG               C^AATTG
MseI         1121  TTAA                 T^TAA
MunI         1937  CAATTG               C^AATTG
NarI          519  GGCGCC               GG^CGCC
NspI          713  GCATGT               [GA]CATG^[CT]
PpuMI        1943  GGGTCCT              [GA]G^G[AT]CC[CT]
SacI         1744  GAGCTC               GAGCT^C
ScaI         1103  AGTACT               AGT^ACT
SfoI          519  GGCGCC               GGC^GCC
SmaI          599  CCCGGG               CCC^GGG
StuI         1541  AGGCCT               AGG^CCT
Tru9I        1121  TTAA                 T^TAA
XmaCI         599  CCCGGG               C^CCGGG
XmaI          599  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

Alw44I       1177  GTGCAC               G^TGCAC
             1491  GTGCAC              
ApaLI        1177  GTGCAC               G^TGCAC
             1491  GTGCAC              
AvaI          599  CCCGGG               C^[CT]CG[GA]G
             1532  CTCGGG              
BsmFI         282  GTCCC                GGGAC(10/14)
             1318  GTCCC               
BsoBI         599  CCCGGG               C^[CT]CG[GA]G
             1532  CTCGGG              
BspMI         379  ACCTGC               ACCTGC(4/8)
              682  ACCTGC              
BsrDI         726  GCAATG               GCAATG(2/0)
             1849  GCAATG              
BsrFI         324  ACCGGC               [GA]^CCGG[CT]
              342  ACCGGC              
BstYI         911  AGATCC               [GA]^GATC[CT]
             1055  AGATCT              
Cfr10I        324  ACCGGC               [GA]^CCGG[CT]
              342  ACCGGC              
EarI           13  GAAGAG               CTCTTC(1/4)
             1172  GAAGAG              
HindIII      1304  AAGCTT               A^AGCTT
             1841  AAGCTT              
Ksp632I        13  GAAGAG               CTCTTC(1/4)
             1172  GAAGAG              
MflI          911  AGATCC               [GA]^GATC[CT]
             1055  AGATCT              
PshAI        1240  GACTTGAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1578  GACCCTGGTC          
SfcI         1336  CTACAG               C^T[GA][CT]AG
             1677  CTGTAG              
SmlI          496  CTCAAG               C^T[CT][GA]AG
             1242  CTTGAG              
TfiI          133  GAATC                G^A[AT]TC
              441  GAATC               
XhoII         911  AGATCC               [GA]^GATC[CT]
             1055  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   92 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AspHI       AvaII       BanI        
BanII       BbsI        BbvI        BcnI        BfaI        
BmyI        BpmI        BpuAI       BsaHI       BsaI        
BsaJI       BseRI       BsgI        BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        KspI        MaeI        MaeII       
MaeIII      MboI        MboII       MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PvuII       RsaI        SacII       Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
Van91I      XcmI        
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