Restriction map for pF1KSDA0280
##################################
# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       AccI        AccII       AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspHI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BclI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsiHKAI     BsiWI       BsmBI       Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BsrBI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstUI       
BstYI       BstZ17I     Bsu36I      CelII       CfoI        
ClaI        CpoI        DpnII       DraI        DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        KasI        
Ksp632I     KspI        MaeI        MamI        MboI        
MfeI        MflI        MluI        MroI        MseI        
MunI        MvnI        NaeI        NarI        NdeI        
NdeII       NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PleI        PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
PstI        PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
SwaI        TaqI        Tru9I       Tsp45I      Tsp509I     
Tth111I     TthHB8I     Van91I      XbaI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   37 enzymes) #
###################################

Acc65I        622  GGTACC               G^GTACC
ApaI          689  GGGCCC               GGGCC^C
Asp718I       622  GGTACC               G^GTACC
AvaI          561  CCCGAG               C^[CT]CG[GA]G
BbsI          216  GTCTTC               GAAGAC(2/6)
BciVI         402  GTATCC               GTATCC(6/5)
BcnI          260  CCGGG                CC^[GC]GG
BpmI          146  CTCCAG               CTGGAG(16/14)
BpuAI         216  GTCTTC               GAAGAC(2/6)
BsaAI         715  TACGTG               [CT]AC^GT[GA]
BsgI           22  GTGCAG               GTGCAG(16/14)
BsiEI         506  CGGTCG               CG[GA][CT]^CG
BsmAI         670  GTCTC                GTCTC(1/5)
BsoBI         561  CCCGAG               C^[CT]CG[GA]G
BspMI         619  GCAGGT               ACCTGC(4/8)
BsrDI         610  GCAATG               GCAATG(2/0)
BsrFI         465  GCCGGT               [GA]^CCGG[CT]
BstEII         70  GGTTACC              G^GT[ACGT]ACC
BstPI          70  GGTTACC              G^GT[ACGT]ACC
Cac8I         595  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr10I        465  GCCGGT               [GA]^CCGG[CT]
DdeI           50  CTAAG                C^T[ACGT]AG
EcoO65I        70  GGTTACC              G^GT[ACGT]ACC
FauI          313  GCGGG                CCCGC(4/6)
HinfI         432  GAATC                G^A[ACGT]TC
HphI          376  TCACC                GGTGA(8/7)
KpnI          622  GGTACC               GGTAC^C
MaeII         716  ACGT                 A^CGT
MboII         217  TCTTC                GAAGA(8/7)
MspA1I         92  CAGCTG               C[AC]G^C[GT]G
NciI          260  CCGGG                CC^[GC]GG
PpuMI         329  AGGTCCC              [GA]G^G[AT]CC[CT]
PvuII          92  CAGCTG               CAG^CTG
SfaNI         286  GCATC                GCATC(5/9)
StuI          197  AGGCCT               AGG^CCT
TfiI          432  GAATC                G^A[AT]TC
XcmI          581  CCATGGGCATGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   16 enzymes) #
###################################

BalI          202  TGGCCA               TGG^CCA
              578  TGGCCA              
BanII          32  GGGCTC               G[GA]GC[CT]^C
              689  GGGCCC              
BsmI          165  GCATTC               GAATGC(1/-1)
              524  GCATTC              
BstAPI        286  GCATCCTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              547  GCACCTGTTGC         
BstF5I        287  CATCC                GGATG(2/0)
              492  CATCC               
BstXI         569  CCAACGGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              581  CCATGGGCATGG        
DraII         329  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              688  AGGGCCC             
EcoO109I      329  AGGTCCC              [GA]G^G[ACGT]CC[CT]
              688  AGGGCCC             
FokI          287  CATCC                GGATG(9/13)
              492  CATCC               
HapII         260  CCGG                 C^CGG
              466  CCGG                
HpaII         260  CCGG                 C^CGG
              466  CCGG                
MaeIII         71  GTTAC                ^GT[ACGT]AC
              347  GTAAC               
MluNI         202  TGGCCA               TGG^CCA
              578  TGGCCA              
MscI          202  TGGCCA               TGG^CCA
              578  TGGCCA              
MspI          260  CCGG                 C^CGG
              466  CCGG                
NcoI          581  CCATGG               C^CATGG
              605  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   36 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AvaII       
BanI        BbvI        BmyI        BsaJI       BsiYI       
BslI        BsmFI       Bsp1286I    BsrI        BssKI       
BstNI       Cfr13I      DsaI        EaeI        EcoRII      
EcoT14I     Fnu4HI      HaeIII      ItaI        MnlI        
MslI        MvaI        MwoI        NlaIII      NlaIV       
RsaI        Sau96I      ScrFI       StyI        TseI        
TspRI       
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