Restriction map for pF1KSDA0280
 (Linear) (Six-base) MAPSORT of: KIAA0280.seq  Check: 8259  from: 1  to: 732



KIAA0280.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

Acc65I G'GTAC_C

Cuts at:      0     622     732 
   Size:        622     110
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     251     732 
   Size:        251     481
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     202     234     732 
   Size:        202      32     498

  Fragments arranged by size: 

                 498     202      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      94     162     435     550     732 
   Size:         94      68     273     115     182

  Fragments arranged by size: 

                 273     182     115      94      68
 
ApaI G_GGCC'C

Cuts at:      0     693     732 
   Size:        693      39
 
AvaI C'yCGr_G

Cuts at:      0     561     732 
   Size:        561     171
 
BanI G'GyrC_C

Cuts at:      0     235     598     622     732 
   Size:        235     363      24     110

  Fragments arranged by size: 

                 363     235     110      24
 
BanII G_rGCy'C

Cuts at:      0      36     693     732 
   Size:         36     657      39

  Fragments arranged by size: 

                 657      39      36
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     209     732 
   Size:        209     523
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     413     732 
   Size:        413     319
 
Bme1580I G_kGCm'C

Cuts at:      0     663     693     722     732 
   Size:        663      30      29      10

  Fragments arranged by size: 

                 663      30      29      10
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     131     244     732 
   Size:        131     113     488

  Fragments arranged by size: 

                 488     131     113
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     732 
   Size:        131     601
 
BsaAI yAC'GTr

Cuts at:      0     717     732 
   Size:        717      15
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     655     685     732 
   Size:        655      30      47

  Fragments arranged by size: 

                 655      47      30
 
BseYI C'CCAG_C

Cuts at:      0     112     425     479     543     641     732 
   Size:        112     313      54      64      98      91

  Fragments arranged by size: 

                 313     112      98      91      64      54
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     732 
   Size:         43     689
 
BsiEI CG_ry'CG

Cuts at:      0     509     732 
   Size:        509     223
 
BsmI GAATG_Cn'

Cuts at:      0     165     524     732 
   Size:        165     359     208

  Fragments arranged by size: 

                 359     208     165
 
Bsp1286I G_dGCh'C

Cuts at:      0      36     663     693     722     732 
   Size:         36     627      30      29      10

  Fragments arranged by size: 

                 627      36      30      29      10
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     610     732 
   Size:        610     122
 
BsrDI GCAATG_nn'

Cuts at:      0     617     732 
   Size:        617     115
 
BsrFI r'CCGG_y

Cuts at:      0     465     732 
   Size:        465     267
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     292     553     732 
   Size:        292     261     179

  Fragments arranged by size: 

                 292     261     179
 
BstEII G'GTnAC_C

Cuts at:      0      70     732 
   Size:         70     662
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     576     588     732 
   Size:        576      12     144

  Fragments arranged by size: 

                 576     144      12
 
BtgI C'CryG_G

Cuts at:      0     503     581     605     732 
   Size:        503      78      24     127

  Fragments arranged by size: 

                 503     127      78      24
 
BtsI GCAGTG_nn'

Cuts at:      0     350     732 
   Size:        350     382
 
EaeI y'GGCC_r

Cuts at:      0     202     395     578     732 
   Size:        202     193     183     154

  Fragments arranged by size: 

                 202     193     183     154
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     131     251     732 
   Size:        131     120     481

  Fragments arranged by size: 

                 481     131     120
 
EcoO109I rG'GnC_Cy

Cuts at:      0     330     689     732 
   Size:        330     359      43

  Fragments arranged by size: 

                 359     330      43
 
KpnI G_GTAC'C

Cuts at:      0     626     732 
   Size:        626     106
 
MscI TGG'CCA

Cuts at:      0     204     580     732 
   Size:        204     376     152

  Fragments arranged by size: 

                 376     204     152
 
MslI CAynn'nnrTG

Cuts at:      0     586     604     610     732 
   Size:        586      18       6     122

  Fragments arranged by size: 

                 586     122      18       6
 
MspA1I CmG'CkG

Cuts at:      0      94     732 
   Size:         94     638
 
NcoI C'CATG_G

Cuts at:      0     581     605     732 
   Size:        581      24     127

  Fragments arranged by size: 

                 581     127      24
 
PasI CC'CwG_GG

Cuts at:      0     444     732 
   Size:        444     288
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     142     174     732 
   Size:        142      32     558

  Fragments arranged by size: 

                 558     142      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     330     732 
   Size:        330     402
 
PspOMI G'GGCC_C

Cuts at:      0     689     732 
   Size:        689      43
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0      27      46      78     262     294     732 
   Size:         27      19      32     184      32     438

  Fragments arranged by size: 

                 438     184      32      32      27      19
 
PvuII CAG'CTG

Cuts at:      0      94     732 
   Size:         94     638
 
StuI AGG'CCT

Cuts at:      0     199     732 
   Size:        199     533
 
StyI C'CwwG_G

Cuts at:      0     581     605     693     732 
   Size:        581      24      88      39

  Fragments arranged by size: 

                 581      88      39      24
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     280     393     732 
   Size:        280     113     339

  Fragments arranged by size: 

                 339     280     113
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      69     101     117     149     558     590     655     687 
   Size:         69      32      16      32     409      32      65      32

Cuts at:    687     732 
   Size:         45

  Fragments arranged by size: 

                 409      69      65      45      32      32      32      32
                  16
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     588     732 
   Size:        588     144
 

 Enzymes that do cut:

   Acc65I     AcuI     AloI    AlwNI     ApaI     AvaI     BanI    BanII
     BbsI    BciVI Bme1580I     BmrI     BpmI    BsaAI    BsaXI    BseYI
     BsgI    BsiEI     BsmI Bsp1286I    BspMI    BsrDI    BsrFI   BstAPI
   BstEII    BstXI     BtgI     BtsI     EaeI  Eco57MI EcoO109I     KpnI
     MscI     MslI   MspA1I     NcoI     PasI     PpiI    PpuMI   PspOMI
     PsrI    PvuII     StuI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI
     BglI    BglII     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI  BsiHKAI    BsiWI    BsmBI
    BspEI    BspHI    BsrBI    BsrGI   BssHII    BssSI    BstBI    BstYI
  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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