Restriction map for pF1KSDA0272
##################################
# NO CUT ENZYMES (  107 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp718I     AspEI       AspI        AvrII       BamHI       
BbrPI       BcgI        BciVI       BclI        BfrI        
BlnI        BsaAI       BsaBI       BsaWI       BseAI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspEI       BspHI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstZ17I     ClaI        
CpoI        DraI        DraIII      Eam1105I    Eco47III    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        HindIII     HpaI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MseI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SbfI        
SexAI       SfiI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       SspI        
SwaI        Tru9I       Tth111I     Van91I      XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   43 enzymes) #
###################################

AccI          562  GTCTAC               GT^[AC][GT]AC
AccII        1550  CGCG                 CG^CG
AcyI         2099  GGCGCC               G[GA]^CG[CT]C
AflIII        236  ACATGT               A^C[GA][CT]GT
AspHI         295  GTGCTC               G[AT]GC[AT]^C
AvaI          439  CCCGAG               C^[CT]CG[GA]G
AviII         958  TGCGCA               TGC^GCA
BbeI         2099  GGCGCC               GGCGC^C
BbvCI        1693  GCTGAGG              CCTCAGC(-5/-2)
BglI         2142  GCCTGACCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII          92  AGATCT               A^GATCT
BsaHI        2099  GGCGCC               G[GA]^CG[CT]C
BsaI          183  GGTCTC               GGTCTC(1/5)
BsiHKAI       295  GTGCTC               G[AT]GC[AT]^C
BsoBI         439  CCCGAG               C^[CT]CG[GA]G
BspLU11I      236  ACATGT               A^CATGT
BspMI        1135  GCAGGT               ACCTGC(4/8)
BssSI          52  CTCGTG               CACGAG(-5/-1)
BstAPI        274  GCAACTCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        1550  CGCG                 CG^CG
BstYI          92  AGATCT               [GA]^GATC[CT]
DrdI          816  GACCCACAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2118  CGGCCG               C^GGCCG
EclXI        2118  CGGCCG               C^GGCCG
Eco52I       2118  CGGCCG               C^GGCCG
FspI          958  TGCGCA               TGC^GCA
HgaI         1548  GACGC                GACGC(5/10)
Hin1I        2099  GGCGCC               G[GA]^CG[CT]C
HincII       1621  GTTGAC               GT[CT]^[GA]AC
HindII       1621  GTTGAC               GT[CT]^[GA]AC
KasI         2099  GGCGCC               G^GCGCC
MflI           92  AGATCT               [GA]^GATC[CT]
MvnI         1550  CGCG                 CG^CG
NarI         2099  GGCGCC               GG^CGCC
SapI          160  GAAGAGC              GCTCTTC(1/4)
SfaNI        2114  GCATC                GCATC(5/9)
SfoI         2099  GGCGCC               GGC^GCC
SphI         2057  GCATGC               GCATG^C
TaqI          158  TCGA                 T^CGA
TfiI          810  GATTC                G^A[AT]TC
TthHB8I       158  TCGA                 T^CGA
XbaI         1080  TCTAGA               T^CTAGA
XhoII          92  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AlwI          155  GGATC                GGATC(4/5)
              713  GGATC               
AocI          844  CCTGAGG              CC^T[ACGT]AGG
             1245  CCTTAGG             
Asp700I       345  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1971  GAAGAAGTTC          
BalI          410  TGGCCA               TGG^CCA
             2063  TGGCCA              
BanI          699  GGTGCC               G^G[CT][GA]CC
             2099  GGCGCC              
BbsI         1123  GAAGAC               GAAGAC(2/6)
             1833  GAAGAC              
BlpI          288  GCTGAGC              GC^T[ACGT]AGC
             1535  GCTCAGC             
Bpu1102I      288  GCTGAGC              GC^T[ACGT]AGC
             1535  GCTCAGC             
BpuAI        1123  GAAGAC               GAAGAC(2/6)
             1833  GAAGAC              
BsiEI         705  CGACCG               CG[GA][CT]^CG
             2118  CGGCCG              
BsmAI         184  GTCTC                GTCTC(1/5)
              825  GTCTC               
BsrBI        1533  CCGCTC               CCGCTC(-3/-3)
             1287  GAGCGG              
BsrDI         395  GCAATG               GCAATG(2/0)
             1466  GCAATG              
BsrI         1159  CCAGT                ACTGG(1/-1)
             1792  CCAGT               
Bsu36I        844  CCTGAGG              CC^T[ACGT]AGG
             1245  CCTTAGG             
CelII         288  GCTGAGC              GC^T[ACGT]AGC
             1535  GCTCAGC             
Eco81I        844  CCTGAGG              CC^T[ACGT]AGG
             1245  CCTTAGG             
MluNI         410  TGGCCA               TGG^CCA
             2063  TGGCCA              
MscI          410  TGGCCA               TGG^CCA
             2063  TGGCCA              
NcoI          491  CCATGG               C^CATGG
              576  CCATGG              
ScaI          762  AGTACT               AGT^ACT
             1178  AGTACT              
Tsp45I        834  GTCAC                ^GT[GC]AC
             1567  GTCAC               
Tsp509I      1093  AATT                 ^AATT
             1380  AATT                
XmnI          345  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1971  GAAGAAGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   84 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       ApaI        
AvaII       BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmFI       Bsp1286I    BsrFI       
BssKI       BstF5I      BstNI       BstXI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MwoI        NaeI        NciI        NdeII       
NgoMIV      NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfcI        SmlI        StuI        
StyI        TseI        TspRI       XcmI        
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