Restriction map for pF1KSDA0251
##################################
# NO CUT ENZYMES (  102 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AviII       AvrII       BalI        
BbeI        BclI        BglI        BglII       BlnI        
BsaHI       BsiWI       BsmBI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrFI       BsrGI       
BssHII      Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      Cfr10I      ClaI        CpoI        
DrdI        Eam1105I    Eco47III    Eco81I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT14I     FauI        
FbaI        FseI        FspI        Hin1I       HindIII     
HpaI        KasI        KpnI        KspI        MfeI        
MluNI       MscI        MunI        NaeI        NarI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PinAI       PmeI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        SbfI        ScaI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SspBI       StyI        SwaI        
XbaI        XcmI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccI         2032  GTCTAC               GT^[AC][GT]AC
AccIII        130  TCCGGA               T^CCGGA
AcsI          380  GAATTT               [GA]^AATT[CT]
AflII        2358  CTTAAG               C^TTAAG
ApoI          380  GAATTT               [GA]^AATT[CT]
BamHI         631  GGATCC               G^GATCC
BanI         1317  GGTGCC               G^G[CT][GA]CC
BbrPI        1362  CACGTG               CAC^GTG
BcgI          948  CGATGACCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         347  GGATAC               GTATCC(6/5)
BfrI         2358  CTTAAG               C^TTAAG
BsaAI        1362  CACGTG               [CT]AC^GT[GA]
BsaBI        1420  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         130  TCCGGA               T^CCGGA
BsgI         2048  GTGCAG               GTGCAG(16/14)
BsiEI        1254  CGGCCG               CG[GA][CT]^CG
BsmI         1847  GCATTC               GAATGC(1/-1)
BspEI         130  TCCGGA               T^CCGGA
BspMI        1519  GCAGGT               ACCTGC(4/8)
BsrDI        1773  CATTGC               GCAATG(2/0)
DraIII       2042  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          901  CCGTGG               C^C[GA][CT]GG
EagI         1254  CGGCCG               C^GGCCG
EclXI        1254  CGGCCG               C^GGCCG
Eco52I       1254  CGGCCG               C^GGCCG
EcoT22I       205  ATGCAT               ATGCA^T
HaeII        2198  AGCGCC               [GA]GCGC^[CT]
MamI         1420  GATGTGGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI            5  ACGCGT               A^CGCGT
MroI          130  TCCGGA               T^CCGGA
NsiI          205  ATGCAT               ATGCA^T
PflMI         362  CCACCTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI        1362  CACGTG               CAC^GTG
PmlI         1362  CACGTG               CAC^GTG
SapI         1710  GAAGAGC              GCTCTTC(1/4)
SmaI         1787  CCCGGG               CCC^GGG
SphI         1074  GCATGC               GCATG^C
SspI          250  AATATT               AAT^ATT
StuI         2294  AGGCCT               AGG^CCT
Van91I        362  CCACCTTATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI        1787  CCCGGG               C^CCGGG
XmaI         1787  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccII           6  CGCG                 CG^CG
             1278  CGCG                
AfaI          610  GTAC                 GT^AC
             1472  GTAC                
AflIII          5  ACGCGT               A^C[GA][CT]GT
             1363  ACGTGT              
Asp700I      1831  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1888  GAACGGCTTC          
AspHI        1150  GAGCTC               G[AT]GC[AT]^C
             1759  GAGCTC              
AspI          284  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1741  GACAACGTC           
BbvCI        2272  CCTCAGC              CCTCAGC(-5/-2)
             1354  GCTGAGG             
BfaI          344  CTAG                 C^TAG
              545  CTAG                
BlpI         1152  GCTCAGC              GC^T[ACGT]AGC
             1756  GCTGAGC             
Bpu1102I     1152  GCTCAGC              GC^T[ACGT]AGC
             1756  GCTGAGC             
BsiHKAI      1150  GAGCTC               G[AT]GC[AT]^C
             1759  GAGCTC              
BssSI        1269  CTCGTG               CACGAG(-5/-1)
             1762  CTCGTG              
BstAPI       1311  GCAGCTGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2041  GCACAAGGTGC         
BstUI           6  CGCG                 CG^CG
             1278  CGCG                
BstXI         153  CCAGGGCAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
              246  CCAAAATATTGG        
CelII        1152  GCTCAGC              GC^T[ACGT]AGC
             1756  GCTGAGC             
CfoI         1279  GCGC                 GCG^C
             2199  GCGC                
EaeI         1254  CGGCCG               [CT]^GGCC[GA]
             1778  CGGCCA              
HhaI         1279  GCGC                 GCG^C
             2199  GCGC                
HinP1I       1279  GCGC                 G^CGC
             2199  GCGC                
HincII       1082  GTCAAC               GT[CT]^[GA]AC
             1091  GTCAAC              
HindII       1082  GTCAAC               GT[CT]^[GA]AC
             1091  GTCAAC              
MaeI          344  CTAG                 C^TAG
              545  CTAG                
MvnI            6  CGCG                 CG^CG
             1278  CGCG                
NdeI          268  CATATG               CA^TATG
              792  CATATG              
NspI         1074  GCATGC               [GA]CATG^[CT]
             1578  ACATGC              
PaeR7I       1806  CTCGAG               C^TCGAG
             2230  CTCGAG              
PflFI         284  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1741  GACAACGTC           
PpuMI        2125  AGGTCCC              [GA]G^G[AT]CC[CT]
             2309  GGGTCCC             
PstI          863  CTGCAG               CTGCA^G
             1870  CTGCAG              
RsaI          610  GTAC                 GT^AC
             1472  GTAC                
SacI         1150  GAGCTC               GAGCT^C
             1759  GAGCTC              
Tth111I       284  GACATAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             1741  GACAACGTC           
XhoI         1806  CTCGAG               C^TCGAG
             2230  CTCGAG              
XmnI         1831  GAAGTGGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1888  GAACGGCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AluI        AlwI        AvaI        AvaII       
BanII       BbsI        BbvI        BcnI        BmyI        
BpmI        BpuAI       BsaI        BsaJI       BsaWI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
BsoBI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DraI        DraII       EarI        EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HgaI        HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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