Restriction map for pF1KSDA0238
##################################
# NO CUT ENZYMES (  100 enzymes) #
##################################

AatII       AccI        AflII       AflIII      AgeI        
AhdI        AlwNI       Aor51HI     ApaI        AscI        
AseI        AsnI        AspEI       AspI        AviII       
AvrII       BbrPI       BbvCI       BfrI        BglII       
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspHI       
BspLU11I    BsrBI       BsrGI       BssHII      BssSI       
Bst1107I    BstBI       BstZ17I     CelII       CpoI        
DraII       DraIII      DrdI        Eam1105I    Eco47III    
EcoO109I    EcoRI       EcoRV       EcoT14I     EcoT22I     
FseI        FspI        HincII      HindII      HindIII     
HpaI        KspI        MamI        MfeI        MluI        
MunI        NaeI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshBI       RcaI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfuI        SgrAI       SmaI        SnaBI       
SpeI        Sse8387I    SspBI       StyI        Tth111I     
Van91I      XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   63 enzymes) #
###################################

Acc65I        296  GGTACC               G^GTACC
AccII        1369  CGCG                 CG^CG
AclI          468  AACGTT               AA^CGTT
AcyI          124  GGCGCC               G[GA]^CG[CT]C
Alw44I       1202  GTGCAC               G^TGCAC
AocI         1896  CCTCAGG              CC^T[ACGT]AGG
ApaLI        1202  GTGCAC               G^TGCAC
Asp700I       280  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       296  GGTACC               G^GTACC
AvaI          527  CTCGAG               C^[CT]CG[GA]G
BalI          953  TGGCCA               TGG^CCA
BbeI          124  GGCGCC               GGCGC^C
BcgI          350  CGATCGCAGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         1490  TGATCA               T^GATCA
BfaI          835  CTAG                 C^TAG
BglI          689  GCCTCCTCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI         124  GGCGCC               G[GA]^CG[CT]C
BsaI          220  GAGACC               GGTCTC(1/5)
BseRI         691  CTCCTC               GAGGAG(10/8)
BsgI         1473  CTGCAC               GTGCAG(16/14)
BsmAI         220  GAGAC                GTCTC(1/5)
BsoBI         527  CTCGAG               C^[CT]CG[GA]G
BspDI        1636  ATCGAT               AT^CGAT
BsrFI        1132  GCCGGT               [GA]^CCGG[CT]
BstAPI       1056  GCAAGGCGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        482  GGTTACC              G^GT[ACGT]ACC
BstPI         482  GGTTACC              G^GT[ACGT]ACC
BstUI        1369  CGCG                 CG^CG
Bsu36I       1896  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1132  GCCGGT               [GA]^CCGG[CT]
ClaI         1636  ATCGAT               AT^CGAT
DraI         1530  TTTAAA               TTT^AAA
EagI         1130  CGGCCG               C^GGCCG
EclXI        1130  CGGCCG               C^GGCCG
Eco52I       1130  CGGCCG               C^GGCCG
Eco81I       1896  CCTCAGG              CC^T[ACGT]AGG
EcoNI         489  CCTGTTTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       482  GGTTACC              G^GT[ACGT]ACC
FauI          784  GCGGG                CCCGC(4/6)
FbaI         1490  TGATCA               T^GATCA
HgaI         1438  GCGTC                GACGC(5/10)
Hin1I         124  GGCGCC               G[GA]^CG[CT]C
KasI          124  GGCGCC               G^GCGCC
KpnI          296  GGTACC               GGTAC^C
MaeI          835  CTAG                 C^TAG
MluNI         953  TGGCCA               TGG^CCA
MscI          953  TGGCCA               TGG^CCA
MvnI         1369  CGCG                 CG^CG
NarI          124  GGCGCC               GG^CGCC
PaeR7I        527  CTCGAG               C^TCGAG
PshAI         996  GACAGATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      468  AACGTT               AA^CGTT
PstI         1516  CTGCAG               CTGCA^G
PvuI          350  CGATCG               CGAT^CG
SapI         1902  GAAGAGC              GCTCTTC(1/4)
SfiI          688  GGCCTCCTCGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI          124  GGCGCC               GGC^GCC
SmlI          527  CTCGAG               C^T[CT][GA]AG
SphI         1145  GCATGC               GCATG^C
SspI          828  AATATT               AAT^ATT
SwaI         1529  ATTTAAAT             ATTT^AAAT
XhoI          527  CTCGAG               C^TCGAG
XmnI          280  GAAGATGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AccIII       1446  TCCGGA               T^CCGGA
             1742  TCCGGA              
BamHI         632  GGATCC               G^GATCC
             1449  GGATCC              
BanI          124  GGCGCC               G^G[CT][GA]CC
              296  GGTACC              
BciVI         624  GTATCC               GTATCC(6/5)
              964  GTATCC              
BpmI          132  CTCCAG               CTGGAG(16/14)
             1724  CTCCAG              
BseAI        1446  TCCGGA               T^CCGGA
             1742  TCCGGA              
BsiEI         350  CGATCG               CG[GA][CT]^CG
             1130  CGGCCG              
BspEI        1446  TCCGGA               T^CCGGA
             1742  TCCGGA              
BspMI        1514  ACCTGC               ACCTGC(4/8)
              479  GCAGGT              
BsrDI         195  CATTGC               GCAATG(2/0)
              813  CATTGC              
CfoI          125  GCGC                 GCG^C
             1427  GCGC                
EaeI          953  TGGCCA               [CT]^GGCC[GA]
             1130  CGGCCG              
EarI          101  CTCTTC               CTCTTC(1/4)
             1902  GAAGAG              
HaeII         124  GGCGCC               [GA]GCGC^[CT]
             1426  AGCGCC              
HhaI          125  GCGC                 GCG^C
             1427  GCGC                
HinP1I        125  GCGC                 G^CGC
             1427  GCGC                
Ksp632I       101  CTCTTC               CTCTTC(1/4)
             1902  GAAGAG              
MaeII         469  ACGT                 A^CGT
             1652  ACGT                
MroI         1446  TCCGGA               T^CCGGA
             1742  TCCGGA              
NspI          334  ACATGC               [GA]CATG^[CT]
             1145  GCATGC              
PvuII         925  CAGCTG               CAG^CTG
             1496  CAGCTG              
SacI          163  GAGCTC               GAGCT^C
              207  GAGCTC              
StuI         1266  AGGCCT               AGG^CCT
             1893  AGGCCT              


#########################################
# 3 OR MORE CUT ENZYMES (   72 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
ApoI        AspHI       AvaII       BanII       BbsI        
BbvI        BcnI        BmyI        BpuAI       BsaJI       
BsaWI       BsiHKAI     BsiYI       BslI        BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Cac8I       Cfr13I      DdeI        
DpnII       DsaI        EcoRII      Fnu4HI      FokI        
HaeIII      HapII       HinfI       HpaII       HphI        
ItaI        MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MspI        
MvaI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PleI        RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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