Restriction map for pF1KSDA0237
 (Linear) (Six-base) MAPSORT of: KIAA0237.seq  Check: 5513  from: 1  to: 924



KIAA0237.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     702     924 
   Size:        702     222
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     924 
   Size:        236     688
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     724     924 
   Size:        724     200
 
AlwNI CAG_nnn'CTG

Cuts at:      0      22     453     675     924 
   Size:         22     431     222     249

  Fragments arranged by size: 

                 431     249     222      22
 
ApaLI G'TGCA_C

Cuts at:      0     478     924 
   Size:        478     446
 
AvaI C'yCGr_G

Cuts at:      0     533     924 
   Size:        533     391
 
BamHI G'GATC_C

Cuts at:      0      85     862     924 
   Size:         85     777      62

  Fragments arranged by size: 

                 777      85      62
 
BanI G'GyrC_C

Cuts at:      0     148     875     924 
   Size:        148     727      49

  Fragments arranged by size: 

                 727     148      49
 
BanII G_rGCy'C

Cuts at:      0      61     924 
   Size:         61     863
 
BbeI G_GCGC'C

Cuts at:      0     879     924 
   Size:        879      45
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     634     652     881     924 
   Size:        634      18     229      43

  Fragments arranged by size: 

                 634     229      43      18
 
BbvCI CC'TCA_GC

Cuts at:      0     796     924 
   Size:        796     128
 
BglI GCCn_nnn'nGGC

Cuts at:      0     616     924 
   Size:        616     308
 
Bme1580I G_kGCm'C

Cuts at:      0     482     924 
   Size:        482     442
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     879     924 
   Size:        879      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24      45     184     532     619     916     924 
   Size:         24      21     139     348      87     297       8

  Fragments arranged by size: 

                 348     297     139      87      24      21       8
 
Bpu10I CC'TnA_GC

Cuts at:      0     796     924 
   Size:        796     128
 
BsaHI Gr'CG_yC

Cuts at:      0     876     924 
   Size:        876      48
 
BsaWI w'CCGG_w

Cuts at:      0     497     924 
   Size:        497     427
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     828     924 
   Size:        828      96
 
BseYI C'CCAG_C

Cuts at:      0      89     109     420     570     924 
   Size:         89      20     311     150     354

  Fragments arranged by size: 

                 354     311     150      89      20
 
BsiHKAI G_wGCw'C

Cuts at:      0      61     196     259     334     482     924 
   Size:         61     135      63      75     148     442

  Fragments arranged by size: 

                 442     148     135      75      63      61
 
Bsp1286I G_dGCh'C

Cuts at:      0      61     196     259     334     482     924 
   Size:         61     135      63      75     148     442

  Fragments arranged by size: 

                 442     148     135      75      63      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     602     702     924 
   Size:        602     100     222

  Fragments arranged by size: 

                 602     222     100
 
BsrDI GCAATG_nn'

Cuts at:      0     482     924 
   Size:        482     442
 
BsrFI r'CCGG_y

Cuts at:      0     811     924 
   Size:        811     113
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     675     924 
   Size:        675     249
 
BstEII G'GTnAC_C

Cuts at:      0     807     924 
   Size:        807     117
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      48     159     924 
   Size:         48     111     765

  Fragments arranged by size: 

                 765     111      48
 
BstYI r'GATC_y

Cuts at:      0      85     862     924 
   Size:         85     777      62

  Fragments arranged by size: 

                 777      85      62
 
BtgI C'CryG_G

Cuts at:      0     467     803     924 
   Size:        467     336     121

  Fragments arranged by size: 

                 467     336     121
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     255     924 
   Size:        255     669
 
EaeI y'GGCC_r

Cuts at:      0     161     504     617     735     924 
   Size:        161     343     113     118     189

  Fragments arranged by size: 

                 343     189     161     118     113
 
EarI CTCTTCn'nnn_

Cuts at:      0     832     924 
   Size:        832      92
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     150     238     924 
   Size:        150      88     686

  Fragments arranged by size: 

                 686     150      88
 
EcoICRI GAG'CTC

Cuts at:      0      59     924 
   Size:         59     865
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      24      45     184     236     532     619     916     924 
   Size:         24      21     139      52     296      87     297       8

  Fragments arranged by size: 

                 297     296     139      87      52      24      21       8
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0      19      35     537     608     691     924 
   Size:         19      16     502      71      83     233

  Fragments arranged by size: 

                 502     233      83      71      19      16
 
FspI TGC'GCA

Cuts at:      0     238     924 
   Size:        238     686
 
HaeII r_GCGC'y

Cuts at:      0     803     879     924 
   Size:        803      76      45

  Fragments arranged by size: 

                 803      76      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     516     548     639     671     924 
   Size:        516      32      91      32     253

  Fragments arranged by size: 

                 516     253      91      32      32
 
KasI G'GCGC_C

Cuts at:      0     875     924 
   Size:        875      49
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     924 
   Size:        374     550
 
MscI TGG'CCA

Cuts at:      0     163     506     619     924 
   Size:        163     343     113     305

  Fragments arranged by size: 

                 343     305     163     113
 
MslI CAynn'nnrTG

Cuts at:      0     274     763     924 
   Size:        274     489     161

  Fragments arranged by size: 

                 489     274     161
 
MspA1I CmG'CkG

Cuts at:      0     510     805     924 
   Size:        510     295     119

  Fragments arranged by size: 

                 510     295     119
 
NarI GG'CG_CC

Cuts at:      0     876     924 
   Size:        876      48
 
NcoI C'CATG_G

Cuts at:      0     467     924 
   Size:        467     457
 
PasI CC'CwG_GG

Cuts at:      0     696     924 
   Size:        696     228
 
PfoI T'CCnGG_A

Cuts at:      0      40     410     924 
   Size:         40     370     514

  Fragments arranged by size: 

                 514     370      40
 
PpuMI rG'GwC_Cy

Cuts at:      0      19     691     924 
   Size:         19     672     233

  Fragments arranged by size: 

                 672     233      19
 
PstI C_TGCA'G

Cuts at:      0     713     924 
   Size:        713     211
 
PvuII CAG'CTG

Cuts at:      0     510     924 
   Size:        510     414
 
SacI G_AGCT'C

Cuts at:      0      61     924 
   Size:         61     863
 
SacII CC_GC'GG

Cuts at:      0     806     924 
   Size:        806     118
 
SanDI GG'GwC_CC

Cuts at:      0     691     924 
   Size:        691     233
 
SfcI C'TryA_G

Cuts at:      0     709     924 
   Size:        709     215
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     616     924 
   Size:        616     308
 
SfoI GGC'GCC

Cuts at:      0     877     924 
   Size:        877      47
 
StyI C'CwwG_G

Cuts at:      0     467     924 
   Size:        467     457
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     136     480     924 
   Size:        136     344     444

  Fragments arranged by size: 

                 444     344     136
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     736     768     924 
   Size:        736      32     156

  Fragments arranged by size: 

                 736     156      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     840     924 
   Size:        840      84
 

 Enzymes that do cut:

     AarI     AcuI     AlfI    AlwNI    ApaLI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BglI Bme1580I     BmrI     BpmI
   Bpu10I    BsaHI    BsaWI    BseRI    BseYI  BsiHKAI Bsp1286I    BspMI
    BsrDI    BsrFI   BstAPI   BstEII    BstXI    BstYI     BtgI    BtgZI
     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FspI    HaeII
    Hin4I     KasI     MmeI     MscI     MslI   MspA1I     NarI     NcoI
     PasI     PfoI    PpuMI     PstI    PvuII     SacI    SacII    SanDI
     SfcI     SfiI     SfoI     StyI    TaqII     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaXI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrGI   BssHII    BssSI    BstBI  BstZ17I   Bsu36I     BtsI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV
     FalI     FseI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SalI     SapI
     SbfI     ScaI    SexAI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI
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