Restriction map for pF1KSDA0235
##################################
# NO CUT ENZYMES (   91 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AocI        
ApaLI       AscI        AspEI       BbeI        BbrPI       
BbvCI       BfrI        BsaHI       BsaWI       BseAI       
BsgI        BsiWI       BsmBI       BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BssHII      BssSI       
BstBI       BstEII      BstPI       Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EagI        Eam1105I    EclXI       Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       FauI        FseI        
Hin1I       HindIII     KasI        KspI        MluI        
MroI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SapI        SbfI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        Sse8387I    
SspI        SwaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   56 enzymes) #
###################################

Acc65I        729  GGTACC               G^GTACC
Aor51HI       563  AGCGCT               AGC^GCT
ApaI          570  GGGCCC               GGGCC^C
AseI         2307  ATTAAT               AT^TAAT
AsnI         2307  ATTAAT               AT^TAAT
Asp718I       729  GGTACC               G^GTACC
AspI         1625  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI           35  CTCGGG               C^[CT]CG[GA]G
AviII        3086  TGCGCA               TGC^GCA
AvrII        3156  CCTAGG               C^CTAGG
BalI         3116  TGGCCA               TGG^CCA
BamHI        1933  GGATCC               G^GATCC
BbsI         2800  GAAGAC               GAAGAC(2/6)
BcgI         3057  GCACAAGATTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1056  GTATCC               GTATCC(6/5)
BclI         1323  TGATCA               T^GATCA
BglI         1469  GCCTTACAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI         3156  CCTAGG               C^CTAGG
BpuAI        2800  GAAGAC               GAAGAC(2/6)
BsaAI        3001  TACGTG               [CT]AC^GT[GA]
BsaI          431  GAGACC               GGTCTC(1/5)
BsiEI        2790  CGGTCG               CG[GA][CT]^CG
BsmI         3185  GAATGC               GAATGC(1/-1)
BsoBI          35  CTCGGG               C^[CT]CG[GA]G
BspHI           6  TCATGA               T^CATGA
Bst1107I      969  GTATAC               GTA^TAC
BstZ17I       969  GTATAC               GTA^TAC
Eco47III      563  AGCGCT               AGC^GCT
EcoT22I       851  ATGCAT               ATGCA^T
FbaI         1323  TGATCA               T^GATCA
FspI         3086  TGCGCA               TGC^GCA
HaeII         563  AGCGCT               [GA]GCGC^[CT]
HgaI         1176  GCGTC                GACGC(5/10)
HpaI          825  GTTAAC               GTT^AAC
HphI          159  TCACC                GGTGA(8/7)
KpnI          729  GGTACC               GGTAC^C
MaeII        3002  ACGT                 A^CGT
MfeI         1502  CAATTG               C^AATTG
MluNI        3116  TGGCCA               TGG^CCA
MscI         3116  TGGCCA               TGG^CCA
MunI         1502  CAATTG               C^AATTG
NsiI          851  ATGCAT               ATGCA^T
PflFI        1625  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI        3175  CCACCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        3159  AGGACCT              [GA]G^G[AT]CC[CT]
PshBI        2307  ATTAAT               AT^TAAT
RcaI            6  TCATGA               T^CATGA
SacI         1373  GAGCTC               GAGCT^C
SalI         2792  GTCGAC               G^TCGAC
ScaI         1299  AGTACT               AGT^ACT
SexAI        1626  ACCTGGT              A^CC[AT]GGT
SmlI         2204  CTCAAG               C^T[CT][GA]AG
Tsp45I       2586  GTCAC                ^GT[GC]AC
Tth111I      1625  GACCTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I       3175  CCACCAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2221  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccI          969  GTATAC               GT^[AC][GT]AC
             2792  GTCGAC              
AccII         248  CGCG                 CG^CG
             2440  CGCG                
AspHI        1373  GAGCTC               G[AT]GC[AT]^C
             2515  GTGCTC              
BanI          729  GGTACC               G^G[CT][GA]CC
             1333  GGTGCC              
BanII         570  GGGCCC               G[GA]GC[CT]^C
             1373  GAGCTC              
BcnI         2078  CCCGG                CC^[GC]GG
             3150  CCCGG               
BglII         196  AGATCT               A^GATCT
             1789  AGATCT              
BsaBI         511  GATTTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1934  GATCCTCATC          
BsiHKAI      1373  GAGCTC               G[AT]GC[AT]^C
             2515  GTGCTC              
Bsp1407I     1781  TGTACA               T^GTACA
             2553  TGTACA              
BsrDI        2680  CATTGC               GCAATG(2/0)
             2870  GCAATG              
BsrGI        1781  TGTACA               T^GTACA
             2553  TGTACA              
BstAPI        898  GCAGGCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1426  GCACAAGCTGC         
BstUI         248  CGCG                 CG^CG
             2440  CGCG                
BstXI        2943  CCAGAATGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2996  CCAATTACGTGG        
DsaI          677  CCATGG               C^C[GA][CT]GG
             1045  CCATGG              
EarI          605  CTCTTC               CTCTTC(1/4)
              741  CTCTTC              
EcoNI         376  CCTGAGAAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             3163  CCTATTGGAGG         
HapII        2079  CCGG                 C^CGG
             3151  CCGG                
HpaII        2079  CCGG                 C^CGG
             3151  CCGG                
Ksp632I       605  CTCTTC               CTCTTC(1/4)
              741  CTCTTC              
MamI          511  GATTTTAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1934  GATCCTCATC          
MslI         2641  CATCCTTATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2973  CACCATGATG          
MspI         2079  CCGG                 C^CGG
             3151  CCGG                
MvnI          248  CGCG                 CG^CG
             2440  CGCG                
NciI         2078  CCCGG                CC^[GC]GG
             3150  CCCGG               
NcoI          677  CCATGG               C^CATGG
             1045  CCATGG              
SphI         1283  GCATGC               GCATG^C
             1868  GCATGC              
SspBI        1781  TGTACA               T^GTACA
             2553  TGTACA              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AvaII       BbvI        
BfaI        BlpI        BmyI        BpmI        Bpu1102I    
BsaJI       BseRI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstYI       Cac8I       CelII       
CfoI        Cfr13I      DdeI        DpnII       DraII       
EaeI        EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HhaI        HinP1I      HincII      
HindII      HinfI       ItaI        MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspA1I      MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp509I     TspRI       TthHB8I     
XhoII       XmnI        
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