Restriction map for pF1KSDA0224
##################################
# NO CUT ENZYMES (   90 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AhdI        AocI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AviII       AvrII       
BbeI        BbrPI       BciVI       BfaI        BfrI        
BlnI        BsaI        BsiWI       BsmI        Bsp1407I    
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     Bsu36I      CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoRI       EcoRV       EcoT22I     FseI        
FspI        HindIII     HpaI        KasI        KpnI        
KspI        MaeI        MfeI        MluI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PinAI       PmaCI       
PmeI        PmlI        PshAI       PshBI       Psp1406I    
RsrII       SacII       SalI        SbfI        SexAI       
SfiI        SfoI        SfuI        SgrAI       SmaI        
SnaBI       SpeI        Sse8387I    SspBI       StuI        
SwaI        XbaI        XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AatII        2427  GACGTC               GACGT^C
AccIII        311  TCCGGA               T^CCGGA
AspI         3199  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         1236  GGTGCC               G^G[CT][GA]CC
BbvCI         274  GCTGAGG              CCTCAGC(-5/-2)
BglI         3581  GCCCCCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI        2871  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         311  TCCGGA               [AT]^CCGG[AT]
BseAI         311  TCCGGA               T^CCGGA
BsiEI         966  CGATCG               CG[GA][CT]^CG
BspEI         311  TCCGGA               T^CCGGA
BspHI        3368  TCATGA               T^CATGA
BsrDI        1326  CATTGC               GCAATG(2/0)
BsrFI        2569  GCCGGC               [GA]^CCGG[CT]
Cfr10I       2569  GCCGGC               [GA]^CCGG[CT]
EcoNI        1812  CCTTGGCGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
MamI         2871  GATGCTCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          311  TCCGGA               T^CCGGA
MseI         2482  TTAA                 T^TAA
NaeI         2569  GCCGGC               GCC^GGC
NgoMIV       2569  GCCGGC               G^CCGGC
PaeR7I        749  CTCGAG               C^TCGAG
PflFI        3199  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI        2711  AGGACCT              [GA]G^G[AT]CC[CT]
PvuI          966  CGATCG               CGAT^CG
RcaI         3368  TCATGA               T^CATGA
SapI         1453  GAAGAGC              GCTCTTC(1/4)
ScaI          779  AGTACT               AGT^ACT
SphI         1142  GCATGC               GCATG^C
SspI         2416  AATATT               AAT^ATT
Tru9I        2482  TTAA                 T^TAA
Tth111I      3199  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI          749  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AccI          636  GTCTAC               GT^[AC][GT]AC
             3600  GTCTAC              
AcsI          908  GAATTT               [GA]^AATT[CT]
             1552  GAATTT              
Alw44I       1225  GTGCAC               G^TGCAC
             2200  GTGCAC              
Aor51HI      1124  AGCGCT               AGC^GCT
             1967  AGCGCT              
ApaLI        1225  GTGCAC               G^TGCAC
             2200  GTGCAC              
ApoI          908  GAATTT               [GA]^AATT[CT]
             1552  GAATTT              
BamHI        1892  GGATCC               G^GATCC
             2783  GGATCC              
BcgI          960  GCAAGCCGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1646  GCATCGTGATCG        
BclI         1871  TGATCA               T^GATCA
             2982  TGATCA              
BglII        2522  AGATCT               A^GATCT
             3608  AGATCT              
BlpI         1093  GCTCAGC              GC^T[ACGT]AGC
             1387  GCTCAGC             
Bpu1102I     1093  GCTCAGC              GC^T[ACGT]AGC
             1387  GCTCAGC             
BspDI          32  ATCGAT               AT^CGAT
             2461  ATCGAT              
BstEII        289  GGTGACC              G^GT[ACGT]ACC
             2262  GGTGACC             
BstPI         289  GGTGACC              G^GT[ACGT]ACC
             2262  GGTGACC             
CelII        1093  GCTCAGC              GC^T[ACGT]AGC
             1387  GCTCAGC             
ClaI           32  ATCGAT               AT^CGAT
             2461  ATCGAT              
DsaI         3512  CCATGG               C^C[GA][CT]GG
             3644  CCATGG              
Eco47III     1124  AGCGCT               AGC^GCT
             1967  AGCGCT              
EcoO65I       289  GGTGACC              G^GT[ACGT]ACC
             2262  GGTGACC             
FbaI         1871  TGATCA               T^GATCA
             2982  TGATCA              
HincII       2131  GTTGAC               GT[CT]^[GA]AC
             2440  GTTGAC              
HindII       2131  GTTGAC               GT[CT]^[GA]AC
             2440  GTTGAC              
NcoI         3512  CCATGG               C^CATGG
             3644  CCATGG              
SacI         2629  GAGCTC               GAGCT^C
             3134  GAGCTC              
XcmI         1307  CCACTTCTGACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2498  CCAGGATTGGCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       ApaI        AspHI       AvaI        
AvaII       BalI        BanII       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaAI       
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrBI       BsrI        
BssKI       BssSI       BstAPI      BstF5I      BstNI       
BstUI       BstXI       BstYI       Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraII       EaeI        
EarI        EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MaeIII      MboI        MboII       MflI        MluNI       
MnlI        MscI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PflMI       PleI        
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmlI        StyI        
TaqI        TfiI        TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     Van91I      XhoII       
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