Restriction map for pF1KSDA0215
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       AccII       AclI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        AspEI       AspI        
AviII       AvrII       BbeI        BbrPI       BclI        
BfrI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaHI       BsiEI       BsiWI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstZ17I     CelII       CfoI        ClaI        
CpoI        DraIII      DrdI        EagI        Eam1105I    
EarI        EclXI       Eco47III    Eco52I      EcoO65I     
EcoRI       EcoRV       FbaI        FseI        FspI        
HaeII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HpaI        KasI        Ksp632I     
KspI        MfeI        MluI        MunI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NspV        PacI        PflFI       PinAI       
PmaCI       PmeI        PmlI        Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tth111I     XmaCI       XmaI        


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# ONE CUT ENZYMES (   54 enzymes) #
###################################

Acc65I        243  GGTACC               G^GTACC
AccI         2243  GTAGAC               GT^[AC][GT]AC
AccIII        131  TCCGGA               T^CCGGA
AcsI           95  AAATTC               [GA]^AATT[CT]
AocI         1744  CCTCAGG              CC^T[ACGT]AGG
ApoI           95  AAATTC               [GA]^AATT[CT]
AseI          178  ATTAAT               AT^TAAT
AsnI          178  ATTAAT               AT^TAAT
Asp700I      1387  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       243  GGTACC               G^GTACC
AvaI         2046  CTCGAG               C^[CT]CG[GA]G
BalI         1817  TGGCCA               TGG^CCA
BamHI         845  GGATCC               G^GATCC
BbvCI         121  GCTGAGG              CCTCAGC(-5/-2)
BcgI         1447  CGAAATCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1776  GTATCC               GTATCC(6/5)
BcnI          326  CCCGG                CC^[GC]GG
BglI         2003  GCCTGGAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         490  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         131  TCCGGA               [AT]^CCGG[AT]
BseAI         131  TCCGGA               T^CCGGA
BsgI         1372  GTGCAG               GTGCAG(16/14)
BsmBI        1058  GAGACG               CGTCTC(1/5)
BsmI         1391  GCATTC               GAATGC(1/-1)
BsoBI        2046  CTCGAG               C^[CT]CG[GA]G
BspEI         131  TCCGGA               T^CCGGA
BsrFI         617  GCCGGT               [GA]^CCGG[CT]
BstAPI        935  GCAACTTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1997  CCAATGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1744  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        617  GCCGGT               [GA]^CCGG[CT]
DraI         2146  TTTAAA               TTT^AAA
Eco81I       1744  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1047  CCTAGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      1417  ATGCAT               ATGCA^T
FauI         1185  GCGGG                CCCGC(4/6)
HindIII      1195  AAGCTT               A^AGCTT
KpnI          243  GGTACC               GGTAC^C
MamI          490  GATGAGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI        1817  TGGCCA               TGG^CCA
MroI          131  TCCGGA               T^CCGGA
MscI         1817  TGGCCA               TGG^CCA
NciI          326  CCCGG                CC^[GC]GG
NheI         1272  GCTAGC               G^CTAGC
NsiI         1417  ATGCAT               ATGCA^T
NspI         1733  ACATGC               [GA]CATG^[CT]
PaeR7I       2046  CTCGAG               C^TCGAG
PshAI          40  GACGAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI         178  ATTAAT               AT^TAAT
PstI         2264  CTGCAG               CTGCA^G
SacI         2273  GAGCTC               GAGCT^C
XbaI         2407  TCTAGA               T^CTAGA
XhoI         2046  CTCGAG               C^TCGAG
XmnI         1387  GAAAGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BanI          243  GGTACC               G^G[CT][GA]CC
             1740  GGTGCC              
BglII         896  AGATCT               A^GATCT
             1514  AGATCT              
BsaI         2152  GAGACC               GGTCTC(1/5)
              620  GGTCTC              
BseRI        1210  GAGGAG               GAGGAG(10/8)
             1213  GAGGAG              
BsmFI         811  GGGAC                GGGAC(10/14)
              709  GTCCC               
BspMI         117  ACCTGC               ACCTGC(4/8)
             1737  GCAGGT              
DsaI         1938  CCATGG               C^C[GA][CT]GG
             2421  CCATGG              
EaeI         1247  TGGCCG               [CT]^GGCC[GA]
             1817  TGGCCA              
NcoI         1938  CCATGG               C^CATGG
             2421  CCATGG              
SfcI         2123  CTGTAG               C^T[GA][CT]AG
             2264  CTGCAG              
StuI           13  AGGCCT               AGG^CCT
              686  AGGCCT              
TaqI         1401  TCGA                 T^CGA
             2047  TCGA                
TthHB8I      1401  TCGA                 T^CGA
             2047  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   78 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AspHI       AvaII       BanII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EcoO109I    EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MwoI        
NdeII       NlaIII      NlaIV       PflMI       PleI        
PpuMI       PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SmlI        StyI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
Van91I      XcmI        XhoII       
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