Restriction map for pF1KSDA0211
##################################
# NO CUT ENZYMES (   73 enzymes) #
##################################

AccI        AclI        AflII       AgeI        Aor51HI     
AscI        AseI        AsnI        Asp700I     AspI        
BbeI        BciVI       BfrI        BlpI        Bpu1102I    
BsaBI       BsiWI       Bsp1407I    BspDI       BspHI       
BspLU11I    BsrGI       BssHII      Bst1107I    BstBI       
BstZ17I     CelII       ClaI        CpoI        DraI        
Eco47III    EcoRI       EcoRV       FseI        HpaI        
KasI        MamI        MfeI        MluI        MunI        
NarI        NheI        NotI        NruI        NspI        
NspV        PacI        PflFI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SalI        SbfI        ScaI        
SfoI        SfuI        SgrAI       SnaBI       SphI        
Sse8387I    SspBI       SspI        SwaI        Tth111I     
XbaI        XcmI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AatII        3234  GACGTC               GACGT^C
AccIII        693  TCCGGA               T^CCGGA
AhdI         2764  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI        2764  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        3083  TGCGCA               TGC^GCA
AvrII         808  CCTAGG               C^CTAGG
BbrPI        1741  CACGTG               CAC^GTG
BglII         294  AGATCT               A^GATCT
BlnI          808  CCTAGG               C^CTAGG
BsaI         3536  GAGACC               GGTCTC(1/5)
BseAI         693  TCCGGA               T^CCGGA
BsmBI        1778  GAGACG               CGTCTC(1/5)
BsmI         2488  GCATTC               GAATGC(1/-1)
BspEI         693  TCCGGA               T^CCGGA
BsrBI        2331  CCGCTC               CCGCTC(-3/-3)
BssSI         652  CACGAG               CACGAG(-5/-1)
BstAPI       1850  GCACACTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII       1220  GGTCACC              G^GT[ACGT]ACC
BstPI        1220  GGTCACC              G^GT[ACGT]ACC
EagI          191  CGGCCG               C^GGCCG
Eam1105I     2764  GACGAGCTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI         191  CGGCCG               C^GGCCG
Eco52I        191  CGGCCG               C^GGCCG
EcoNI        1297  CCTGAGGCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1220  GGTCACC              G^GT[ACGT]ACC
EcoT22I      3202  ATGCAT               ATGCA^T
FspI         3083  TGCGCA               TGC^GCA
HaeII        1234  AGCGCC               [GA]GCGC^[CT]
HincII       3749  GTCAAC               GT[CT]^[GA]AC
HindII       3749  GTCAAC               GT[CT]^[GA]AC
HindIII       719  AAGCTT               A^AGCTT
KspI         2926  CCGCGG               CCGC^GG
MroI          693  TCCGGA               T^CCGGA
MseI         1139  TTAA                 T^TAA
NsiI         3202  ATGCAT               ATGCA^T
PmaCI        1741  CACGTG               CAC^GTG
PmlI         1741  CACGTG               CAC^GTG
SacII        2926  CCGCGG               CCGC^GG
SfiI          867  GGCCTTGCAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI         2845  ACTAGT               A^CTAGT
Tru9I        1139  TTAA                 T^TAA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

Acc65I        787  GGTACC               G^GTACC
             3035  GGTACC              
AccII        2927  CGCG                 CG^CG
             3378  CGCG                
AcsI          325  AAATTT               [GA]^AATT[CT]
             1311  AAATTC              
AcyI         2668  GGCGTC               G[GA]^CG[CT]C
             3234  GACGTC              
AflIII       2727  ACACGT               A^C[GA][CT]GT
             2993  ACGTGT              
Alw44I       1507  GTGCAC               G^TGCAC
             1848  GTGCAC              
ApaLI        1507  GTGCAC               G^TGCAC
             1848  GTGCAC              
ApoI          325  AAATTT               [GA]^AATT[CT]
             1311  AAATTC              
Asp718I       787  GGTACC               G^GTACC
             3035  GGTACC              
BcgI         3695  CGAGGAGATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1922  GCAAGGGGCTCG        
BclI          591  TGATCA               T^GATCA
             3741  TGATCA              
BsaAI        1741  CACGTG               [CT]AC^GT[GA]
             2992  TACGTG              
BsaHI        2668  GGCGTC               G[GA]^CG[CT]C
             3234  GACGTC              
BsiEI         191  CGGCCG               CG[GA][CT]^CG
             3018  CGGTCG              
BsrDI        1239  CATTGC               GCAATG(2/0)
             3326  GCAATG              
BstUI        2927  CGCG                 CG^CG
             3378  CGCG                
DrdI         1557  GACAGCCAAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2164  GACTACATGGTC        
FbaI          591  TGATCA               T^GATCA
             3741  TGATCA              
Hin1I        2668  GGCGTC               G[GA]^CG[CT]C
             3234  GACGTC              
KpnI          787  GGTACC               GGTAC^C
             3035  GGTACC              
MvnI         2927  CGCG                 CG^CG
             3378  CGCG                
NcoI         2498  CCATGG               C^CATGG
             3629  CCATGG              
NdeI          135  CATATG               CA^TATG
              583  CATATG              
PaeR7I       2819  CTCGAG               C^TCGAG
             3409  CTCGAG              
PflMI        1947  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3292  CCAGTCAGTGG         
SexAI        1625  ACCAGGT              A^CC[AT]GGT
             3437  ACCAGGT             
SmaI          776  CCCGGG               CCC^GGG
             1400  CCCGGG              
StuI         2893  AGGCCT               AGG^CCT
             3758  AGGCCT              
Van91I       1947  CCAGCTGCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3292  CCAGTCAGTGG         
XhoI         2819  CTCGAG               C^TCGAG
             3409  CTCGAG              
XmaCI         776  CCCGGG               C^CCGGG
             1400  CCCGGG              
XmaI          776  CCCGGG               C^CCGGG
             1400  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  112 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaI        AvaII       
BalI        BamHI       BanI        BanII       BbsI        
BbvCI       BbvI        BcnI        BfaI        BglI        
BmyI        BpmI        BpuAI       BsaJI       BsaWI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstXI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
DraIII      DsaI        EaeI        EarI        Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeIII      HapII       HgaI        HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NaeI        NciI        NdeII       NgoMIV      NlaIII      
NlaIV       PleI        PpuMI       PstI        PvuII       
RsaI        SapI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SmlI        StyI        TaqI        
TfiI        TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XhoII       
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