Restriction map for pF1KSDA0211
 (Linear) (Six-base) MAPSORT of: KIAA0211.seq  Check: 1036  from: 1  to: 3801



KIAA0211.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2652    3139    3801 
   Size:       2652     487     662

  Fragments arranged by size: 

                2652     662     487
 
AatII G_ACGT'C

Cuts at:      0    3238    3801 
   Size:       3238     563
 
Acc65I G'GTAC_C

Cuts at:      0     787    3035    3801 
   Size:        787    2248     766

  Fragments arranged by size: 

                2248     787     766
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1553    2771    2918    3801 
   Size:       1553    1218     147     883

  Fragments arranged by size: 

                1553    1218     883     147
 
AflIII A'CryG_T

Cuts at:      0    2727    2993    3801 
   Size:       2727     266     808

  Fragments arranged by size: 

                2727     808     266
 
AhdI GACnn_n'nnGTC

Cuts at:      0    2769    3801 
   Size:       2769    1032
 
AleI CACnn'nnGTG

Cuts at:      0     317    1907    2411    2483    2848    3801 
   Size:        317    1590     504      72     365     953

  Fragments arranged by size: 

                1590     953     504     365     317      72
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1477    2887    3801 
   Size:       1477    1410     914

  Fragments arranged by size: 

                1477    1410     914
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1966    1998    3047    3079    3801 
   Size:       1966      32    1049      32     722

  Fragments arranged by size: 

                1966    1049     722      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     445    1192    1953    2107    2241    2264    3801 
   Size:        445     747     761     154     134      23    1537

  Fragments arranged by size: 

                1537     761     747     445     154     134      23
 
ApaI G_GGCC'C

Cuts at:      0     941    1400    3712    3801 
   Size:        941     459    2312      89

  Fragments arranged by size: 

                2312     941     459      89
 
ApaLI G'TGCA_C

Cuts at:      0    1507    1848    3801 
   Size:       1507     341    1953

  Fragments arranged by size: 

                1953    1507     341
 
ApoI r'AATT_y

Cuts at:      0     325    1311    3801 
   Size:        325     986    2490

  Fragments arranged by size: 

                2490     986     325
 
AvaI C'yCGr_G

Cuts at:      0     776    1400    2819    3402    3409    3801 
   Size:        776     624    1419     583       7     392

  Fragments arranged by size: 

                1419     776     624     583     392       7
 
AvrII C'CTAG_G

Cuts at:      0     808    3801 
   Size:        808    2993
 
BamHI G'GATC_C

Cuts at:      0     450     690    1178    1736    3801 
   Size:        450     240     488     558    2065

  Fragments arranged by size: 

                2065     558     488     450     240
 
BanI G'GyrC_C

Cuts at:      0     787    1275    1611    2010    3035    3319    3624    3801 
   Size:        787     488     336     399    1025     284     305     177

  Fragments arranged by size: 

                1025     787     488     399     336     305     284     177
            
 
BanII G_rGCy'C

Cuts at:      0     292     941    1003    1324    1400    1931    3410    3613 
   Size:        292     649      62     321      76     531    1479     203

Cuts at:   3613    3712    3801 
   Size:         99      89

  Fragments arranged by size: 

                1479     649     531     321     292     203      99      89
                  76      62
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1559    1653    2598    2664    2777    3801 
   Size:       1559      94     945      66     113    1024

  Fragments arranged by size: 

                1559    1024     945     113      94      66
 
BbvCI CC'TCA_GC

Cuts at:      0    2932    3431    3502    3801 
   Size:       2932     499      71     299

  Fragments arranged by size: 

                2932     499     299      71
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1911    1945    3684    3718    3801 
   Size:       1911      34    1739      34      83

  Fragments arranged by size: 

                1911    1739      83      34      34
 
BclI T'GATC_A

Cuts at:      0     591    3741    3801 
   Size:        591    3150      60

  Fragments arranged by size: 

                3150     591      60
 
BfrBI ATG'CAT

Cuts at:      0    3204    3801 
   Size:       3204     597
 
BglI GCCn_nnn'nGGC

Cuts at:      0      82     865     874    2813    3801 
   Size:         82     783       9    1939     988

  Fragments arranged by size: 

                1939     988     783      82       9
 
BglII A'GATC_T

Cuts at:      0     294    3801 
   Size:        294    3507
 
Bme1580I G_kGCm'C

Cuts at:      0     941    1400    1511    1616    1697    1852    2271    2683 
   Size:        941     459     111     105      81     155     419     412

Cuts at:   2683    2692    3629    3712    3801 
   Size:          9     937      83      89

  Fragments arranged by size: 

                 941     937     459     419     412     155     111     105
                  89      83      81       9
 
BmgBI CAC'GTC

Cuts at:      0    2730    3801 
   Size:       2730    1071
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      92     257     535    3801 
   Size:         92     165     278    3266

  Fragments arranged by size: 

                3266     278     165      92
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    3627    3801 
   Size:       3627     174
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     287     400     417     568    1027    1789    2066 
   Size:        113     174     113      17     151     459     762     277

Cuts at:   2066    2764    3202    3299    3801 
   Size:        698     438      97     502

  Fragments arranged by size: 

                 762     698     502     459     438     277     174     151
                 113     113      97      17
 
Bpu10I CC'TnA_GC

Cuts at:      0    1804    2932    3431    3502    3801 
   Size:       1804    1128     499      71     299

  Fragments arranged by size: 

                1804    1128     499     299      71
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2787    3801 
   Size:       2787    1014
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    3530    3801 
   Size:       3530     271
 
BsaAI yAC'GTr

Cuts at:      0    1743    2994    3801 
   Size:       1743    1251     807

  Fragments arranged by size: 

                1743    1251     807
 
BsaHI Gr'CG_yC

Cuts at:      0    2669    3235    3801 
   Size:       2669     566     566

  Fragments arranged by size: 

                2669     566     566
 
BsaWI w'CCGG_w

Cuts at:      0     567     693    2120    3801 
   Size:        567     126    1427    1681

  Fragments arranged by size: 

                1681    1427     567     126
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     527     557    2554    2584    3801 
   Size:        527      30    1997      30    1217

  Fragments arranged by size: 

                1997    1217     527      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118    2069    2218    3457    3559    3562    3565    3574 
   Size:        118    1951     149    1239     102       3       3       9

Cuts at:   3574    3619    3634    3711    3801 
   Size:         45      15      77      90

  Fragments arranged by size: 

                1951    1239     149     118     102      90      77      45
                  15       9       3       3
 
BseYI C'CCAG_C

Cuts at:      0    1252    1381    1946    2022    2543    2555    2573    2885 
   Size:       1252     129     565      76     521      12      18     312

Cuts at:   2885    3157    3705    3801 
   Size:        272     548      96

  Fragments arranged by size: 

                1252     565     548     521     312     272     129      96
                  76      18      12
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1908    2165    2277    2356    2725    3126    3801 
   Size:       1908     257     112      79     369     401     675

  Fragments arranged by size: 

                1908     675     401     369     257     112      79
 
BsiEI CG_ry'CG

Cuts at:      0     194    3021    3801 
   Size:        194    2827     780

  Fragments arranged by size: 

                2827     780     194
 
BsiHKAI G_wGCw'C

Cuts at:      0    1292    1456    1511    1852    1861    2593    2740    3801 
   Size:       1292     164      55     341       9     732     147    1061

  Fragments arranged by size: 

                1292    1061     732     341     164     147      55       9
            
 
BsmI GAATG_Cn'

Cuts at:      0    2488    3801 
   Size:       2488    1313
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1772    3801 
   Size:       1772    2029
 
Bsp1286I G_dGCh'C

Cuts at:      0     292     941    1003    1292    1324    1400    1456    1511 
   Size:        292     649      62     289      32      76      56      55

Cuts at:   1511    1616    1697    1852    1861    1931    2271    2593    2683 
   Size:        105      81     155       9      70     340     322      90

Cuts at:   2683    2692    2740    3410    3613    3629    3712    3801 
   Size:          9      48     670     203      16      83      89

  Fragments arranged by size: 

                 670     649     340     322     292     289     203     155
                 105      90      89      83      81      76      70      62
                  56      55      48      32      16       9       9
 
BspEI T'CCGG_A

Cuts at:      0     693    3801 
   Size:        693    3108
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     799    2230    2652    2959    3139    3801 
   Size:        799    1431     422     307     180     662

  Fragments arranged by size: 

                1431     799     662     422     307     180
 
BsrBI CCG'CTC

Cuts at:      0    2333    3801 
   Size:       2333    1468
 
BsrDI GCAATG_nn'

Cuts at:      0    1238    3333    3801 
   Size:       1238    2095     468

  Fragments arranged by size: 

                2095    1238     468
 
BsrFI r'CCGG_y

Cuts at:      0     189    1746    1820    3044    3664    3801 
   Size:        189    1557      74    1224     620     137

  Fragments arranged by size: 

                1557    1224     620     189     137      74
 
BssSI C'ACGA_G

Cuts at:      0     652    3801 
   Size:        652    3149
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1856    3801 
   Size:       1856    1945
 
BstEII G'GTnAC_C

Cuts at:      0    1220    3801 
   Size:       1220    2581
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     319     885    1816    2850    3801 
   Size:        319     566     931    1034     951

  Fragments arranged by size: 

                1034     951     931     566     319
 
BstYI r'GATC_y

Cuts at:      0     294     306     450     690     927    1178    1188    1736 
   Size:        294      12     144     240     237     251      10     548

Cuts at:   1736    3801 
   Size:       2065

  Fragments arranged by size: 

                2065     548     294     251     240     237     144      12
                  10
 
Bsu36I CC'TnA_GG

Cuts at:      0     446    1298    3269    3719    3794    3801 
   Size:        446     852    1971     450      75       7

  Fragments arranged by size: 

                1971     852     450     446      75       7
 
BtgI C'CryG_G

Cuts at:      0    2006    2498    2742    2926    3015    3629    3801 
   Size:       2006     492     244     184      89     614     172

  Fragments arranged by size: 

                2006     614     492     244     184     172      89
 
BtsI GCAGTG_nn'

Cuts at:      0    1065    1459    1945    2070    2463    2522    3055    3590 
   Size:       1065     394     486     125     393      59     533     535

Cuts at:   3590    3801 
   Size:        211

  Fragments arranged by size: 

                1065     535     533     486     394     393     211     125
                  59
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0      51      86    3801 
   Size:         51      35    3715

  Fragments arranged by size: 

                3715      51      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     100    1629    1856    3441    3531    3801 
   Size:        100    1529     227    1585      90     270

  Fragments arranged by size: 

                1585    1529     270     227     100      90
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1563    2170    3801 
   Size:       1563     607    1631

  Fragments arranged by size: 

                1631    1563     607
 
EaeI y'GGCC_r

Cuts at:      0     191     915    1514    1748    1818    2880    3732    3801 
   Size:        191     724     599     234      70    1062     852      69

  Fragments arranged by size: 

                1062     852     724     599     234     191      70      69
            
 
EagI C'GGCC_G

Cuts at:      0     191    3801 
   Size:        191    3610
 
EarI CTCTTCn'nnn_

Cuts at:      0    1280    1792    1879    2611    3348    3520    3551    3801 
   Size:       1280     512      87     732     737     172      31     250

  Fragments arranged by size: 

                1280     737     732     512     250     172      87      31
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1839    3801 
   Size:       1839    1962
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     287     400     417     568    1027    1553    1789 
   Size:        113     174     113      17     151     459     526     236

Cuts at:   1789    2066    2764    2771    2918    3202    3299    3801 
   Size:        277     698       7     147     284      97     502

  Fragments arranged by size: 

                 698     526     502     459     284     277     236     174
                 151     147     113     113      97      17       7
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1301    3801 
   Size:       1301    2500
 
EcoO109I rG'GnC_Cy

Cuts at:      0     937    1248    1397    1404    1671    2322    3801 
   Size:        937     311     149       7     267     651    1479

  Fragments arranged by size: 

                1479     937     651     311     267     149       7
 
FspI TGC'GCA

Cuts at:      0    3085    3801 
   Size:       3085     716
 
HaeII r_GCGC'y

Cuts at:      0    1238    3801 
   Size:       1238    2563
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     527     559    1360    1392    2755    2785    2787    2817 
   Size:        527      32     801      32    1363      30       2      30

Cuts at:   2817    3003    3035    3661    3693    3801 
   Size:        186      32     626      32     108

  Fragments arranged by size: 

                1363     801     626     527     186     108      32      32
                  32      32      30      30       2
 
HincII GTy'rAC

Cuts at:      0    3751    3801 
   Size:       3751      50
 
HindIII A'AGCT_T

Cuts at:      0     719    3801 
   Size:        719    3082
 
KpnI G_GTAC'C

Cuts at:      0     791    3039    3801 
   Size:        791    2248     762

  Fragments arranged by size: 

                2248     791     762
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1709    3001    3801 
   Size:       1709    1292     800

  Fragments arranged by size: 

                1709    1292     800
 
MscI TGG'CCA

Cuts at:      0     917    1516    3734    3801 
   Size:        917     599    2218      67

  Fragments arranged by size: 

                2218     917     599      67
 
MslI CAynn'nnrTG

Cuts at:      0     139     317    1907    2411    2483    2848    3801 
   Size:        139     178    1590     504      72     365     953

  Fragments arranged by size: 

                1590     953     504     365     178     139      72
 
MspA1I CmG'CkG

Cuts at:      0    1385    1950    2277    2791    2885    2928    3574    3682 
   Size:       1385     565     327     514      94      43     646     108

Cuts at:   3682    3801 
   Size:        119

  Fragments arranged by size: 

                1385     646     565     514     327     119     108      94
                  43
 
NaeI GCC'GGC

Cuts at:      0     191    1748    1822    3046    3801 
   Size:        191    1557      74    1224     755

  Fragments arranged by size: 

                1557    1224     755     191      74
 
NcoI C'CATG_G

Cuts at:      0    2498    3629    3801 
   Size:       2498    1131     172

  Fragments arranged by size: 

                2498    1131     172
 
NdeI CA'TA_TG

Cuts at:      0     136     584    3801 
   Size:        136     448    3217

  Fragments arranged by size: 

                3217     448     136
 
NgoMIV G'CCGG_C

Cuts at:      0     189    1746    1820    3044    3801 
   Size:        189    1557      74    1224     757

  Fragments arranged by size: 

                1557    1224     757     189      74
 
NsiI A_TGCA'T

Cuts at:      0    3206    3801 
   Size:       3206     595
 
PasI CC'CwG_GG

Cuts at:      0    1326    3801 
   Size:       1326    2475
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1953    3298    3801 
   Size:       1953    1345     503

  Fragments arranged by size: 

                1953    1345     503
 
PfoI T'CCnGG_A

Cuts at:      0     545    2453    3801 
   Size:        545    1908    1348

  Fragments arranged by size: 

                1908    1348     545
 
PmlI CAC'GTG

Cuts at:      0    1743    3801 
   Size:       1743    2058
 
PpuMI rG'GwC_Cy

Cuts at:      0    1404    1671    2322    3801 
   Size:       1404     267     651    1479

  Fragments arranged by size: 

                1479    1404     651     267
 
PsiI TTA'TAA

Cuts at:      0    2585    3801 
   Size:       2585    1216
 
PspOMI G'GGCC_C

Cuts at:      0     937    1396    3708    3801 
   Size:        937     459    2312      93

  Fragments arranged by size: 

                2312     937     459      93
 
PspXI vC'TCGA_Gb

Cuts at:      0    3409    3801 
   Size:       3409     392
 
PstI C_TGCA'G

Cuts at:      0    1060    1390    1466    2529    3801 
   Size:       1060     330      76    1063    1272

  Fragments arranged by size: 

                1272    1063    1060     330      76
 
PvuII CAG'CTG

Cuts at:      0    1385    1950    3682    3801 
   Size:       1385     565    1732     119

  Fragments arranged by size: 

                1732    1385     565     119
 
SacII CC_GC'GG

Cuts at:      0    2929    3801 
   Size:       2929     872
 
SanDI GG'GwC_CC

Cuts at:      0    1404    3801 
   Size:       1404    2397
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1280    1792    1879    3801 
   Size:       1280     512      87    1922

  Fragments arranged by size: 

                1922    1280     512      87
 
SexAI A'CCwGG_T

Cuts at:      0    1625    3437    3801 
   Size:       1625    1812     364

  Fragments arranged by size: 

                1812    1625     364
 
SfcI C'TryA_G

Cuts at:      0    1056    1386    1462    2394    2525    3801 
   Size:       1056     330      76     932     131    1276

  Fragments arranged by size: 

                1276    1056     932     330     131      76
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     874    3801 
   Size:        874    2927
 
SmaI CCC'GGG

Cuts at:      0     778    1402    3801 
   Size:        778     624    2399

  Fragments arranged by size: 

                2399     778     624
 
SmlI C'TyrA_G

Cuts at:      0    2802    2819    3409    3801 
   Size:       2802      17     590     392

  Fragments arranged by size: 

                2802     590     392      17
 
SpeI A'CTAG_T

Cuts at:      0    2845    3801 
   Size:       2845     956
 
StuI AGG'CCT

Cuts at:      0    2895    3760    3801 
   Size:       2895     865      41

  Fragments arranged by size: 

                2895     865      41
 
StyI C'CwwG_G

Cuts at:      0      77     632     764     808     860    2429    2498    3629 
   Size:         77     555     132      44      52    1569      69    1131

Cuts at:   3629    3801 
   Size:        172

  Fragments arranged by size: 

                1569    1131     555     172     132      77      69      52
                  44
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     111     509    2557    2569    2755    3801 
   Size:        111     398    2048      12     186    1046

  Fragments arranged by size: 

                2048    1046     398     186     111      12
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    2554    2586    3772    3801 
   Size:       2554      32    1186      29

  Fragments arranged by size: 

                2554    1186      32      29
 
XhoI C'TCGA_G

Cuts at:      0    2819    3409    3801 
   Size:       2819     590     392

  Fragments arranged by size: 

                2819     590     392
 
XmaI C'CCGG_G

Cuts at:      0     776    1400    3801 
   Size:        776     624    2401

  Fragments arranged by size: 

                2401     776     624
 
ZraI GAC'GTC

Cuts at:      0    3236    3801 
   Size:       3236     565
 

 Enzymes that do cut:

     AarI    AatII   Acc65I     AcuI   AflIII     AhdI     AleI     AlfI
     AloI    AlwNI     ApaI    ApaLI     ApoI     AvaI    AvrII    BamHI
     BanI    BanII     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI   BstEII    BstXI    BstYI   Bsu36I
     BtgI     BtsI    CspCI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I     FspI    HaeII    Hin4I   HincII
  HindIII     KpnI     MmeI     MscI     MslI   MspA1I     NaeI     NcoI
     NdeI   NgoMIV     NsiI     PasI    PflMI     PfoI     PmlI    PpuMI
     PsiI   PspOMI    PspXI     PstI    PvuII    SacII    SanDI     SapI
    SexAI     SfcI     SfiI     SmaI     SmlI     SpeI     StuI     StyI
    TaqII     TstI     XhoI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI     AclI     AfeI    AflII     AgeI     AscI     AseI    AsiSI
     BaeI     BbeI    BciVI     BlpI     BmtI    BsaBI    BsiWI    BspHI
    BsrGI   BssHII    BstBI  BstZ17I    BtgZI     ClaI     DraI  EcoICRI
    EcoRI    EcoRV     FalI     FseI    FspAI     HpaI     KasI     MfeI
     MluI     NarI     NheI     NotI     NruI     NspI     PacI     PciI
     PmeI     PpiI    PshAI     PsrI     PvuI    RsrII     SacI     SalI
     SbfI     ScaI     SfoI     SgfI    SgrAI    SnaBI     SphI     SrfI
     SspI     SwaI     TatI  Tth111I     XbaI     XcmI     XmnI
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