Restriction map for pF1KSDA0210
##################################
# NO CUT ENZYMES (  126 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflIII      AgeI        AocI        Aor51HI     
AscI        Asp718I     AspI        AviII       AvrII       
BamHI       BanI        BbeI        BbrPI       BbsI        
BcgI        BciVI       BcnI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
DsaI        EagI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRV       FauI        
FseI        FspI        HaeII       HgaI        HhaI        
Hin1I       HinP1I      HpaI        KasI        KpnI        
KspI        MamI        MluI        MroI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PvuI        RsrII       
SacI        SacII       SalI        SapI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SwaI        Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AclI          507  AACGTT               AA^CGTT
AflII        1216  CTTAAG               C^TTAAG
AhdI           64  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I       2115  GTGCAC               G^TGCAC
AlwNI        1543  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         2289  GGGCCC               GGGCC^C
ApaLI        2115  GTGCAC               G^TGCAC
AspEI          64  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI          378  CCCGAG               C^[CT]CG[GA]G
BalI          393  TGGCCA               TGG^CCA
BclI          410  TGATCA               T^GATCA
BfrI         1216  CTTAAG               C^TTAAG
BsaI         1368  GGTCTC               GGTCTC(1/5)
BsoBI         378  CCCGAG               C^[CT]CG[GA]G
Bsp1407I     1200  TGTACA               T^GTACA
BspHI        1316  TCATGA               T^CATGA
BsrGI        1200  TGTACA               T^GTACA
BssSI        1301  CACGAG               CACGAG(-5/-1)
BstAPI        260  GCAGCATTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Eam1105I       64  GACCCCTTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I      1177  ATGCAT               ATGCA^T
FbaI          410  TGATCA               T^GATCA
HincII        901  GTCAAC               GT[CT]^[GA]AC
HindII        901  GTCAAC               GT[CT]^[GA]AC
HindIII       665  AAGCTT               A^AGCTT
MluNI         393  TGGCCA               TGG^CCA
MscI          393  TGGCCA               TGG^CCA
NsiI         1177  ATGCAT               ATGCA^T
PshAI         301  GACAAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I      507  AACGTT               AA^CGTT
PvuII         238  CAGCTG               CAG^CTG
RcaI         1316  TCATGA               T^CATGA
SbfI         1032  CCTGCAGG             CCTGCA^GG
Sse8387I     1032  CCTGCAGG             CCTGCA^GG
SspBI        1200  TGTACA               T^GTACA
SspI         1855  AATATT               AAT^ATT
StuI         2093  AGGCCT               AGG^CCT
TaqI          477  TCGA                 T^CGA
TthHB8I       477  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AfaI         1201  GTAC                 GT^AC
             1444  GTAC                
AseI          229  ATTAAT               AT^TAAT
             1384  ATTAAT              
AsnI          229  ATTAAT               AT^TAAT
             1384  ATTAAT              
Asp700I       122  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              450  GAACAAATTC          
AspHI        1346  GTGCTC               G[AT]GC[AT]^C
             2115  GTGCAC              
BanII        2184  GGGCTC               G[GA]GC[CT]^C
             2289  GGGCCC              
BsgI          689  CTGCAC               GTGCAG(16/14)
             1430  GTGCAG              
BsiHKAI      1346  GTGCTC               G[AT]GC[AT]^C
             2115  GTGCAC              
BsmAI        1163  GTCTC                GTCTC(1/5)
             1369  GTCTC               
BsmFI         148  GGGAC                GGGAC(10/14)
             2372  GTCCC               
BspMI         251  GCAGGT               ACCTGC(4/8)
             1432  GCAGGT              
BsrDI        2007  CATTGC               GCAATG(2/0)
             1414  GCAATG              
EcoRI        1648  GAATTC               G^AATTC
             2130  GAATTC              
EcoT14I       787  CCTTGG               C^C[AT][AT]GG
             2055  CCAAGG              
HapII        1329  CCGG                 C^CGG
             2195  CCGG                
HpaII        1329  CCGG                 C^CGG
             2195  CCGG                
MaeII         508  ACGT                 A^CGT
             1710  ACGT                
MfeI          881  CAATTG               C^AATTG
             1576  CAATTG              
MspA1I        238  CAGCTG               C[AC]G^C[GT]G
             2031  CAGCGG              
MspI         1329  CCGG                 C^CGG
             2195  CCGG                
MunI          881  CAATTG               C^AATTG
             1576  CAATTG              
PpuMI        1037  AGGACCT              [GA]G^G[AT]CC[CT]
             1492  AGGACCT             
PshBI         229  ATTAAT               AT^TAAT
             1384  ATTAAT              
PstI           34  CTGCAG               CTGCA^G
             1033  CTGCAG              
RsaI         1201  GTAC                 GT^AC
             1444  GTAC                
SmlI          296  CTCAAG               C^T[CT][GA]AG
             1216  CTTAAG              
SphI          614  GCATGC               GCATG^C
             1565  GCATGC              
StyI          787  CCTTGG               C^C[AT][AT]GG
             2055  CCAAGG              
Tsp45I        656  GTGAC                ^GT[GC]AC
             1261  GTGAC               
XmnI          122  GAACAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              450  GAACAAATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   63 enzymes) #
#########################################

AciI        AcsI        AluI        AlwI        ApoI        
AvaII       BbvCI       BbvI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiYI       BslI        
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
Fnu4HI      FokI        HaeIII      HinfI       HphI        
ItaI        Ksp632I     MaeI        MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
NspI        PleI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TfiI        Tru9I       TseI        
Tsp509I     TspRI       XhoII       
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