Restriction map for pF1KSDA0210
 (Linear) (Six-base) MAPSORT of: KIAA0210.seq  Check: 2369  from: 1  to: 2385



KIAA0210.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1423    2385 
   Size:       1423     962
 
AclI AA'CG_TT

Cuts at:      0     508    2385 
   Size:        508    1877
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     164     946    1772    2084    2385 
   Size:        164     782     826     312     301

  Fragments arranged by size: 

                 826     782     312     301     164
 
AflII C'TTAA_G

Cuts at:      0    1216    2385 
   Size:       1216    1169
 
AhdI GACnn_n'nnGTC

Cuts at:      0      69    2385 
   Size:         69    2316
 
AleI CACnn'nnGTG

Cuts at:      0    2365    2385 
   Size:       2365      20
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1588    2385 
   Size:       1588     797
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1548    2385 
   Size:       1548     837
 
ApaI G_GGCC'C

Cuts at:      0    2293    2385 
   Size:       2293      92
 
ApaLI G'TGCA_C

Cuts at:      0    2115    2385 
   Size:       2115     270
 
ApoI r'AATT_y

Cuts at:      0     454     551    1281    1648    1772    1881    1901    2130 
   Size:        454      97     730     367     124     109      20     229

Cuts at:   2130    2385 
   Size:        255

  Fragments arranged by size: 

                 730     454     367     255     229     124     109      97
                  20
 
AseI AT'TA_AT

Cuts at:      0     230    1385    2385 
   Size:        230    1155    1000

  Fragments arranged by size: 

                1155    1000     230
 
AvaI C'yCGr_G

Cuts at:      0     378    2385 
   Size:        378    2007
 
BanII G_rGCy'C

Cuts at:      0    2188    2293    2385 
   Size:       2188     105      92

  Fragments arranged by size: 

                2188     105      92
 
BbvCI CC'TCA_GC

Cuts at:      0    1078    1497    2298    2385 
   Size:       1078     419     801      87

  Fragments arranged by size: 

                1078     801     419      87
 
BclI T'GATC_A

Cuts at:      0     410    2385 
   Size:        410    1975
 
BfrBI ATG'CAT

Cuts at:      0    1179    2385 
   Size:       1179    1206
 
Bme1580I G_kGCm'C

Cuts at:      0    2119    2293    2385 
   Size:       2119     174      92

  Fragments arranged by size: 

                2119     174      92
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     692    2245    2385 
   Size:        692    1553     140

  Fragments arranged by size: 

                1553     692     140
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1330    2385 
   Size:       1330    1055
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113    1406    1469    1658    2013    2292    2307    2385 
   Size:        113    1293      63     189     355     279      15      78

  Fragments arranged by size: 

                1293     355     279     189     113      78      63      15
            
 
Bpu10I CC'TnA_GC

Cuts at:      0    1078    1497    2298    2385 
   Size:       1078     419     801      87

  Fragments arranged by size: 

                1078     801     419      87
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    2385 
   Size:        281    2104
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1374    2385 
   Size:       1374    1011
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    1644    1674    2011    2041    2305    2335    2385 
   Size:       1644      30     337      30     264      30      50

  Fragments arranged by size: 

                1644     337     264      50      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     110    1319    1458    1461    2385 
   Size:        110    1209     139       3     924

  Fragments arranged by size: 

                1209     924     139     110       3
 
BseYI C'CCAG_C

Cuts at:      0    1410    2204    2292    2317    2385 
   Size:       1410     794      88      25      68

  Fragments arranged by size: 

                1410     794      88      68      25
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     674    1451    2385 
   Size:        674     777     934

  Fragments arranged by size: 

                 934     777     674
 
BsiHKAI G_wGCw'C

Cuts at:      0    1350    2119    2385 
   Size:       1350     769     266

  Fragments arranged by size: 

                1350     769     266
 
Bsp1286I G_dGCh'C

Cuts at:      0    1350    2119    2188    2293    2385 
   Size:       1350     769      69     105      92

  Fragments arranged by size: 

                1350     769     105      92      69
 
BspHI T'CATG_A

Cuts at:      0    1316    2385 
   Size:       1316    1069
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     242    1423    2385 
   Size:        242    1181     962

  Fragments arranged by size: 

                1181     962     242
 
BsrDI GCAATG_nn'

Cuts at:      0    1421    2006    2385 
   Size:       1421     585     379

  Fragments arranged by size: 

                1421     585     379
 
BsrGI T'GTAC_A

Cuts at:      0    1200    2385 
   Size:       1200    1185
 
BssSI C'ACGA_G

Cuts at:      0    1301    2385 
   Size:       1301    1084
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     266    2385 
   Size:        266    2119
 
BstYI r'GATC_y

Cuts at:      0     908    1236    2277    2385 
   Size:        908     328    1041     108

  Fragments arranged by size: 

                1041     908     328     108
 
BtsI GCAGTG_nn'

Cuts at:      0     761    1434    2181    2385 
   Size:        761     673     747     204

  Fragments arranged by size: 

                 761     747     673     204
 
EaeI y'GGCC_r

Cuts at:      0     393    2034    2196    2385 
   Size:        393    1641     162     189

  Fragments arranged by size: 

                1641     393     189     162
 
EarI CTCTTCn'nnn_

Cuts at:      0    1638    1982    2103    2216    2385 
   Size:       1638     344     121     113     169

  Fragments arranged by size: 

                1638     344     169     121     113
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     113     164     946    1406    1469    1658    1772    2013 
   Size:        113      51     782     460      63     189     114     241

Cuts at:   2013    2084    2292    2307    2385 
   Size:         71     208      15      78

  Fragments arranged by size: 

                 782     460     241     208     189     114     113      78
                  71      63      51      15
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1038    1493    1506    2247    2385 
   Size:       1038     455      13     741     138

  Fragments arranged by size: 

                1038     741     455     138      13
 
EcoRI G'AATT_C

Cuts at:      0    1648    2130    2385 
   Size:       1648     482     255

  Fragments arranged by size: 

                1648     482     255
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     149     460    2385 
   Size:        149     311    1925

  Fragments arranged by size: 

                1925     311     149
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      87     402     434     900     932    1189    1221 
   Size:         55      32     315      32     466      32     257      32

Cuts at:   1221    1827    1859    2385 
   Size:        606      32     526

  Fragments arranged by size: 

                 606     526     466     315     257      55      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0     903    2385 
   Size:        903    1482
 
HindIII A'AGCT_T

Cuts at:      0     665    2385 
   Size:        665    1720
 
MfeI C'AATT_G

Cuts at:      0     881    1576    2385 
   Size:        881     695     809

  Fragments arranged by size: 

                 881     809     695
 
MscI TGG'CCA

Cuts at:      0     395    2385 
   Size:        395    1990
 
MslI CAynn'nnrTG

Cuts at:      0     680    1570    2365    2385 
   Size:        680     890     795      20

  Fragments arranged by size: 

                 890     795     680      20
 
MspA1I CmG'CkG

Cuts at:      0     240    2033    2385 
   Size:        240    1793     352

  Fragments arranged by size: 

                1793     352     240
 
NsiI A_TGCA'T

Cuts at:      0    1181    2385 
   Size:       1181    1204
 
NspI r_CATG'y

Cuts at:      0     618     679     954    1569    2385 
   Size:        618      61     275     615     816

  Fragments arranged by size: 

                 816     618     615     275      61
 
PfoI T'CCnGG_A

Cuts at:      0     911    2385 
   Size:        911    1474
 
PpuMI rG'GwC_Cy

Cuts at:      0    1038    1493    2385 
   Size:       1038     455     892

  Fragments arranged by size: 

                1038     892     455
 
PshAI GACnn'nnGTC

Cuts at:      0     305    2385 
   Size:        305    2080
 
PsiI TTA'TAA

Cuts at:      0     605    2385 
   Size:        605    1780
 
PspOMI G'GGCC_C

Cuts at:      0    2289    2385 
   Size:       2289      96
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     114     146    2385 
   Size:        114      32    2239

  Fragments arranged by size: 

                2239     114      32
 
PstI C_TGCA'G

Cuts at:      0      38    1037    2385 
   Size:         38     999    1348

  Fragments arranged by size: 

                1348     999      38
 
PvuII CAG'CTG

Cuts at:      0     240    2385 
   Size:        240    2145
 
SbfI CC_TGCA'GG

Cuts at:      0    1037    2385 
   Size:       1037    1348
 
SfcI C'TryA_G

Cuts at:      0      34     162     635    1033    2385 
   Size:         34     128     473     398    1352

  Fragments arranged by size: 

                1352     473     398     128      34
 
SmlI C'TyrA_G

Cuts at:      0     296    1216    2385 
   Size:        296     920    1169

  Fragments arranged by size: 

                1169     920     296
 
SphI G_CATG'C

Cuts at:      0     618    1569    2385 
   Size:        618     951     816

  Fragments arranged by size: 

                 951     816     618
 
SspI AAT'ATT

Cuts at:      0    1857    2385 
   Size:       1857     528
 
StuI AGG'CCT

Cuts at:      0    2095    2385 
   Size:       2095     290
 
StyI C'CwwG_G

Cuts at:      0     787    2055    2385 
   Size:        787    1268     330

  Fragments arranged by size: 

                1268     787     330
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     990    2385 
   Size:        990    1395
 
TatI w'GTAC_w

Cuts at:      0    1200    2385 
   Size:       1200    1185
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1644    1676    1798    1830    2011    2043    2385 
   Size:       1644      32     122      32     181      32     342

  Fragments arranged by size: 

                1644     342     181     122      32      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     126     454    2385 
   Size:        126     328    1931

  Fragments arranged by size: 

                1931     328     126
 

 Enzymes that do cut:

     AarI     AclI     AcuI    AflII     AhdI     AleI     AlfI    AlwNI
     ApaI    ApaLI     ApoI     AseI     AvaI    BanII    BbvCI     BclI
    BfrBI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI Bsp1286I    BspHI    BspMI
    BsrDI    BsrGI    BssSI   BstAPI    BstYI     BtsI     EaeI     EarI
  Eco57MI EcoO109I    EcoRI     FalI    Hin4I   HincII  HindIII     MfeI
     MscI     MslI   MspA1I     NsiI     NspI     PfoI    PpuMI    PshAI
     PsiI   PspOMI     PsrI     PstI    PvuII     SbfI     SfcI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AfeI   AflIII     AgeI     AloI     AscI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI     BcgI
    BciVI     BglI    BglII     BlpI    BmgBI     BmtI    BsaAI    BsaBI
    BsaHI    BsaWI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BsrBI
    BsrFI   BssHII    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EciI
  EcoICRI    EcoNI    EcoRV     FseI     FspI    FspAI    HaeII     HpaI
     KasI     KpnI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PciI    PflMI
     PmeI     PmlI     PpiI    PspXI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SapI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SwaI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI
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