Restriction map for pF1KSDA0202
##################################
# NO CUT ENZYMES (  128 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp718I     AspEI       
AspI        AvaI        AvrII       BalI        BanI        
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsoBI       Bsp1407I    
BspEI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     CelII       CpoI        DraI        DraIII      
EagI        Eam1105I    EclXI       Eco52I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
HgaI        Hin1I       HindIII     HpaI        KasI        
KpnI        KspI        MfeI        MluI        MluNI       
MroI        MscI        MunI        NarI        NcoI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        Sse8387I    SspBI       
SspI        SwaI        Tth111I     Van91I      XcmI        
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AccII         865  CGCG                 CG^CG
AfaI          771  GTAC                 GT^AC
AlwNI        1255  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1260  CCTGAGG              CC^T[ACGT]AGG
Aor51HI        20  AGCGCT               AGC^GCT
Asp700I       990  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII         375  TGCGCA               TGC^GCA
BamHI         446  GGATCC               G^GATCC
BbsI          856  GAAGAC               GAAGAC(2/6)
BglI          474  GCCCACAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI         856  GAAGAC               GAAGAC(2/6)
BspDI         370  ATCGAT               AT^CGAT
BspHI         689  TCATGA               T^CATGA
BspMI         789  GCAGGT               ACCTGC(4/8)
BsrBI         417  CCGCTC               CCGCTC(-3/-3)
BstAPI       1230  GCAGGCCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         865  CGCG                 CG^CG
BstXI           8  CCACCGACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1260  CCTGAGG              CC^T[ACGT]AGG
ClaI          370  ATCGAT               AT^CGAT
DraII        1217  AGGCCCT              [GA]G^G[ACGT]CC[CT]
DrdI           94  GACCAGCTGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III       20  AGCGCT               AGC^GCT
Eco81I       1260  CCTGAGG              CC^T[ACGT]AGG
EcoNI        1260  CCTGAGGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1217  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       557  CCAAGG               C^C[AT][AT]GG
FspI          375  TGCGCA               TGC^GCA
HinfI         804  GAATC                G^A[ACGT]TC
MseI          680  TTAA                 T^TAA
MslI          174  CACACTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I         97  CAGCTG               C[AC]G^C[GT]G
MvnI          865  CGCG                 CG^CG
NaeI          881  GCCGGC               GCC^GGC
NgoMIV        881  GCCGGC               G^CCGGC
NspI          235  GCATGC               [GA]CATG^[CT]
PvuII          97  CAGCTG               CAG^CTG
RcaI          689  TCATGA               T^CATGA
RsaI          771  GTAC                 GT^AC
SapI          423  GCTCTTC              GCTCTTC(1/4)
SmlI          289  CTCAAG               C^T[CT][GA]AG
SphI          235  GCATGC               GCATG^C
StuI         1232  AGGCCT               AGG^CCT
StyI          557  CCAAGG               C^C[AT][AT]GG
TfiI          804  GAATC                G^A[AT]TC
Tru9I         680  TTAA                 T^TAA
Tsp509I       388  AATT                 ^AATT
XbaI          498  TCTAGA               T^CTAGA
XmnI          990  GAAGGAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BglII         501  AGATCT               A^GATCT
              638  AGATCT              
BsaBI         600  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              834  GATGTTGATC          
BsaI          154  GAGACC               GGTCTC(1/5)
             1180  GAGACC              
BsgI          283  GTGCAG               GTGCAG(16/14)
              787  GTGCAG              
BsmI           30  GAATGC               GAATGC(1/-1)
              693  GAATGC              
EaeI            5  CGGCCA               [CT]^GGCC[GA]
               66  CGGCCA              
FauI          828  GCGGG                CCCGC(4/6)
             1122  GCGGG               
HincII        361  GTTGAC               GT[CT]^[GA]AC
             1045  GTCAAC              
HindII        361  GTTGAC               GT[CT]^[GA]AC
             1045  GTCAAC              
MaeII         281  ACGT                 A^CGT
              452  ACGT                
MamI          600  GATCAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              834  GATGTTGATC          
SfaNI         307  GATGC                GCATC(5/9)
              373  GATGC               
TspRI         176  CACTG                CA[GC]TG[ACGT][ACGT]^
              808  CACTG               


#########################################
# 3 OR MORE CUT ENZYMES (   68 enzymes) #
#########################################

AciI        AluI        AlwI        AspHI       AvaII       
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaJI       BseRI       BsiHKAI     BsiYI       
BslI        BsmAI       Bsp1286I    BsrFI       BsrI        
BssKI       BstF5I      BstNI       BstYI       Cac8I       
CfoI        Cfr10I      Cfr13I      DdeI        DpnII       
DsaI        EarI        EcoRII      Fnu4HI      FokI        
HaeII       HaeIII      HapII       HhaI        HinP1I      
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeIII      MboI        MboII       MflI        MnlI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PstI        SacI        Sau3AI      
Sau96I      ScrFI       SfcI        TaqI        TseI        
Tsp45I      TthHB8I     XhoII       
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