Restriction map for pF1KSDA0202
 (Linear) (Six-base) MAPSORT of: KIAA0202.seq  Check: 7251  from: 1  to: 1287



KIAA0202.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     780    1287 
   Size:        780     507
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     427     511     942    1096    1138    1287 
   Size:        111     316      84     431     154      42     149

  Fragments arranged by size: 

                 431     316     154     149     111      84      42
 
AfeI AGC'GCT

Cuts at:      0      22    1287 
   Size:         22    1265
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1260    1287 
   Size:       1260      27
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     754     787    1287 
   Size:        754      33     500

  Fragments arranged by size: 

                 754     500      33
 
BamHI G'GATC_C

Cuts at:      0     446    1287 
   Size:        446     841
 
BanII G_rGCy'C

Cuts at:      0      47     587     896    1097    1287 
   Size:         47     540     309     201     190

  Fragments arranged by size: 

                 540     309     201     190      47
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     863    1287 
   Size:        863     424
 
BglI GCCn_nnn'nGGC

Cuts at:      0     480    1287 
   Size:        480     807
 
BglII A'GATC_T

Cuts at:      0     501     638    1287 
   Size:        501     137     649

  Fragments arranged by size: 

                 649     501     137
 
Bme1580I G_kGCm'C

Cuts at:      0     485    1287 
   Size:        485     802
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      42    1287 
   Size:         42    1245
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     253    1090    1192    1287 
   Size:         37     216     837     102      95

  Fragments arranged by size: 

                 837     216     102      95      37
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274    1287 
   Size:        274    1013
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     148    1174    1287 
   Size:        148    1026     113

  Fragments arranged by size: 

                1026     148     113
 
BsaBI GATnn'nnATC

Cuts at:      0     604     838    1287 
   Size:        604     234     449

  Fragments arranged by size: 

                 604     449     234
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     556     586    1287 
   Size:        556      30     701

  Fragments arranged by size: 

                 701     556      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     415     745     931    1150    1189    1192    1287 
   Size:        415     330     186     219      39       3      95

  Fragments arranged by size: 

                 415     330     219     186      95      39       3
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     304     808    1287 
   Size:        304     504     479

  Fragments arranged by size: 

                 504     479     304
 
BsiHKAI G_wGCw'C

Cuts at:      0     587     765     896    1097    1115    1287 
   Size:        587     178     131     201      18     172

  Fragments arranged by size: 

                 587     201     178     172     131      18
 
BsmI GAATG_Cn'

Cuts at:      0      36     699    1287 
   Size:         36     663     588

  Fragments arranged by size: 

                 663     588      36
 
Bsp1286I G_dGCh'C

Cuts at:      0      47     485     587     765     896    1097    1115    1287 
   Size:         47     438     102     178     131     201      18     172

  Fragments arranged by size: 

                 438     201     178     172     131     102      47      18
            
 
BspHI T'CATG_A

Cuts at:      0     689    1287 
   Size:        689     598
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     780    1287 
   Size:        780     507
 
BsrBI CCG'CTC

Cuts at:      0     419    1287 
   Size:        419     868
 
BsrFI r'CCGG_y

Cuts at:      0     157     881     899    1287 
   Size:        157     724      18     388

  Fragments arranged by size: 

                 724     388     157      18
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1236    1287 
   Size:       1236      51
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      15    1287 
   Size:         15    1272
 
BstYI r'GATC_y

Cuts at:      0     410     446     501     638    1287 
   Size:        410      36      55     137     649

  Fragments arranged by size: 

                 649     410     137      55      36
 
Bsu36I CC'TnA_GG

Cuts at:      0    1261    1287 
   Size:       1261      26
 
BtgI C'CryG_G

Cuts at:      0     311     653     713    1287 
   Size:        311     342      60     574

  Fragments arranged by size: 

                 574     342     311      60
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     537     953    1287 
   Size:        537     416     334

  Fragments arranged by size: 

                 537     416     334
 
BtsI GCAGTG_nn'

Cuts at:      0     807    1287 
   Size:        807     480
 
ClaI AT'CG_AT

Cuts at:      0     371    1287 
   Size:        371     916
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     100    1287 
   Size:        100    1187
 
EaeI y'GGCC_r

Cuts at:      0       5      66    1287 
   Size:          5      61    1221

  Fragments arranged by size: 

                1221      61       5
 
EarI CTCTTCn'nnn_

Cuts at:      0     196     430    1019    1287 
   Size:        196     234     589     268

  Fragments arranged by size: 

                 589     268     234     196
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     403    1287 
   Size:        403     884
 
EcoICRI GAG'CTC

Cuts at:      0     585     894    1095    1287 
   Size:        585     309     201     192

  Fragments arranged by size: 

                 585     309     201     192
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     111     253     427     511     942    1090    1096 
   Size:         37      74     142     174      84     431     148       6

Cuts at:   1096    1138    1192    1287 
   Size:         42      54      95

  Fragments arranged by size: 

                 431     174     148     142      95      84      74      54
                  42      37       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1264    1287 
   Size:       1264      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1218    1287 
   Size:       1218      69
 
FspI TGC'GCA

Cuts at:      0     377    1287 
   Size:        377     910
 
HaeII r_GCGC'y

Cuts at:      0      24     906    1164    1287 
   Size:         24     882     258     123

  Fragments arranged by size: 

                 882     258     123      24
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     556     588    1287 
   Size:        556      32     699

  Fragments arranged by size: 

                 699     556      32
 
HincII GTy'rAC

Cuts at:      0     363    1047    1287 
   Size:        363     684     240

  Fragments arranged by size: 

                 684     363     240
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     893    1287 
   Size:        893     394
 
MslI CAynn'nnrTG

Cuts at:      0     178    1287 
   Size:        178    1109
 
MspA1I CmG'CkG

Cuts at:      0      99    1287 
   Size:         99    1188
 
NaeI GCC'GGC

Cuts at:      0     883    1287 
   Size:        883     404
 
NgoMIV G'CCGG_C

Cuts at:      0     881    1287 
   Size:        881     406
 
NspI r_CATG'y

Cuts at:      0     239    1287 
   Size:        239    1048
 
PasI CC'CwG_GG

Cuts at:      0      60     776    1287 
   Size:         60     716     511

  Fragments arranged by size: 

                 716     511      60
 
PfoI T'CCnGG_A

Cuts at:      0     269    1287 
   Size:        269    1018
 
PstI C_TGCA'G

Cuts at:      0     398    1013    1226    1287 
   Size:        398     615     213      61

  Fragments arranged by size: 

                 615     398     213      61
 
PvuII CAG'CTG

Cuts at:      0      99    1287 
   Size:         99    1188
 
SacI G_AGCT'C

Cuts at:      0     587     896    1097    1287 
   Size:        587     309     201     190

  Fragments arranged by size: 

                 587     309     201     190
 
SapI GCTCTTCn'nnn_

Cuts at:      0     430    1287 
   Size:        430     857
 
SfcI C'TryA_G

Cuts at:      0     394    1009    1222    1287 
   Size:        394     615     213      65

  Fragments arranged by size: 

                 615     394     213      65
 
SmlI C'TyrA_G

Cuts at:      0     289    1287 
   Size:        289     998
 
SphI G_CATG'C

Cuts at:      0     239    1287 
   Size:        239    1048
 
StuI AGG'CCT

Cuts at:      0    1234    1287 
   Size:       1234      53
 
StyI C'CwwG_G

Cuts at:      0     557    1287 
   Size:        557     730
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     102    1287 
   Size:        102    1185
 
XbaI T'CTAG_A

Cuts at:      0     498    1287 
   Size:        498     789
 
XmnI GAAnn'nnTTC

Cuts at:      0     994    1287 
   Size:        994     293
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     BaeI    BamHI    BanII     BbsI
     BglI    BglII Bme1580I     BplI     BpmI    BpuEI     BsaI    BsaBI
    BsaXI    BseRI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrFI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I     FspI    HaeII    Hin4I   HincII     MmeI     MslI
   MspA1I     NaeI   NgoMIV     NspI     PasI     PfoI     PstI    PvuII
     SacI     SapI     SfcI     SmlI     SphI     StuI     StyI    TaqII
     XbaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BlpI    BmgBI     BmrI     BmtI   Bpu10I    BsaAI
    BsaHI    BsaWI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BsrDI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I    CspCI     DraI
   DraIII     EagI    EcoRI    EcoRV     FalI     FseI    FspAI  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     NarI     NcoI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     SwaI     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     ZraI
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