Restriction map for pF1KSDA0201
 (Linear) (Six-base) MAPSORT of: KIAA0201.seq  Check: 1499  from: 1  to: 2574



KIAA0201.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394    1259    1550    2036    2147    2181    2574 
   Size:        394     865     291     486     111      34     393

  Fragments arranged by size: 

                 865     486     394     393     291     111      34
 
AflIII A'CryG_T

Cuts at:      0    1835    2383    2574 
   Size:       1835     548     191

  Fragments arranged by size: 

                1835     548     191
 
AgeI A'CCGG_T

Cuts at:      0      95    2574 
   Size:         95    2479
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2192    2574 
   Size:       2192     382
 
AlwNI CAG_nnn'CTG

Cuts at:      0     451    1579    1708    2014    2166    2574 
   Size:        451    1128     129     306     152     408

  Fragments arranged by size: 

                1128     451     408     306     152     129
 
ApaLI G'TGCA_C

Cuts at:      0      99    2574 
   Size:         99    2475
 
ApoI r'AATT_y

Cuts at:      0     545     739    1037    1066    1171    1963    1989    2550 
   Size:        545     194     298      29     105     792      26     561

Cuts at:   2550    2574 
   Size:         24

  Fragments arranged by size: 

                 792     561     545     298     194     105      29      26
                  24
 
AseI AT'TA_AT

Cuts at:      0    2083    2574 
   Size:       2083     491
 
AvaI C'yCGr_G

Cuts at:      0      53    2574 
   Size:         53    2521
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     781     814    2574 
   Size:        781      33    1760

  Fragments arranged by size: 

                1760     781      33
 
BamHI G'GATC_C

Cuts at:      0    2328    2574 
   Size:       2328     246
 
BanII G_rGCy'C

Cuts at:      0      28     835    2574 
   Size:         28     807    1739

  Fragments arranged by size: 

                1739     807      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1517    2024    2492    2574 
   Size:       1517     507     468      82

  Fragments arranged by size: 

                1517     507     468      82
 
BclI T'GATC_A

Cuts at:      0    2322    2574 
   Size:       2322     252
 
Bme1580I G_kGCm'C

Cuts at:      0     103    2574 
   Size:        103    2471
 
BmgBI CAC'GTC

Cuts at:      0      21    2574 
   Size:         21    2553
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     676    2574 
   Size:        676    1898
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1727    2084    2574 
   Size:       1727     357     490

  Fragments arranged by size: 

                1727     490     357
 
Bpu10I CC'TnA_GC

Cuts at:      0    2303    2345    2574 
   Size:       2303      42     229

  Fragments arranged by size: 

                2303     229      42
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     279     382    2574 
   Size:        279     103    2192

  Fragments arranged by size: 

                2192     279     103
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      64    1551    2574 
   Size:         64    1487    1023

  Fragments arranged by size: 

                1487    1023      64
 
BsaAI yAC'GTr

Cuts at:      0    2341    2574 
   Size:       2341     233
 
BsaBI GATnn'nnATC

Cuts at:      0    2327    2574 
   Size:       2327     247
 
BsaWI w'CCGG_w

Cuts at:      0      95     892    2574 
   Size:         95     797    1682

  Fragments arranged by size: 

                1682     797      95
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     770     800    2574 
   Size:        770      30    1774

  Fragments arranged by size: 

                1774     770      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1525    1993    2574 
   Size:       1525     468     581

  Fragments arranged by size: 

                1525     581     468
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     461     755    1028    1388    2574 
   Size:        461     294     273     360    1186

  Fragments arranged by size: 

                1186     461     360     294     273
 
BsiEI CG_ry'CG

Cuts at:      0      96    2574 
   Size:         96    2478
 
BsiHKAI G_wGCw'C

Cuts at:      0     103     786     835    2346    2574 
   Size:        103     683      49    1511     228

  Fragments arranged by size: 

                1511     683     228     103      49
 
BsmI GAATG_Cn'

Cuts at:      0     220     871    2304    2574 
   Size:        220     651    1433     270

  Fragments arranged by size: 

                1433     651     270     220
 
Bsp1286I G_dGCh'C

Cuts at:      0      28     103     786     835    2346    2574 
   Size:         28      75     683      49    1511     228

  Fragments arranged by size: 

                1511     683     228      75      49      28
 
BspEI T'CCGG_A

Cuts at:      0     892    2574 
   Size:        892    1682
 
BspHI T'CATG_A

Cuts at:      0    1248    2294    2574 
   Size:       1248    1046     280

  Fragments arranged by size: 

                1248    1046     280
 
BsrFI r'CCGG_y

Cuts at:      0      95    2574 
   Size:         95    2479
 
BsrGI T'GTAC_A

Cuts at:      0     311    2574 
   Size:        311    2263
 
BstYI r'GATC_y

Cuts at:      0     558    2328    2574 
   Size:        558    1770     246

  Fragments arranged by size: 

                1770     558     246
 
BtgI C'CryG_G

Cuts at:      0    1458    2574 
   Size:       1458    1116
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      27      60    2574 
   Size:         27      33    2514

  Fragments arranged by size: 

                2514      33      27
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1929    1964    2574 
   Size:       1929      35     610

  Fragments arranged by size: 

                1929     610      35
 
DraI TTT'AAA

Cuts at:      0     744    1161    2574 
   Size:        744     417    1413

  Fragments arranged by size: 

                1413     744     417
 
EaeI y'GGCC_r

Cuts at:      0     213    2134    2574 
   Size:        213    1921     440

  Fragments arranged by size: 

                1921     440     213
 
EcoICRI GAG'CTC

Cuts at:      0     833    2574 
   Size:        833    1741
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394    1259    1550    1727    2036    2084    2147    2181 
   Size:        394     865     291     177     309      48      63      34

Cuts at:   2181    2574 
   Size:        393

  Fragments arranged by size: 

                 865     394     393     309     291     177      63      48
                  34
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     689    2574 
   Size:        689    1885
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1312    2574 
   Size:       1312    1262
 
EcoRI G'AATT_C

Cuts at:      0    1037    2574 
   Size:       1037    1537
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     273     976    1331    1846    2574 
   Size:        273     703     355     515     728

  Fragments arranged by size: 

                 728     703     515     355     273
 
HaeII r_GCGC'y

Cuts at:      0    1031    2574 
   Size:       1031    1543
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      10      42     445     477     754     786    1421    1453 
   Size:         10      32     403      32     277      32     635      32

Cuts at:   1453    2307    2339    2574 
   Size:        854      32     235

  Fragments arranged by size: 

                 854     635     403     277     235      32      32      32
                  32      32      10
 
HincII GTy'rAC

Cuts at:      0     365    1452    1734    2067    2574 
   Size:        365    1087     282     333     507

  Fragments arranged by size: 

                1087     507     365     333     282
 
HindIII A'AGCT_T

Cuts at:      0    1328    2574 
   Size:       1328    1246
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     276     740     953    1002    2054    2574 
   Size:        276     464     213      49    1052     520

  Fragments arranged by size: 

                1052     520     464     276     213      49
 
MslI CAynn'nnrTG

Cuts at:      0     731    1134    2228    2522    2574 
   Size:        731     403    1094     294      52

  Fragments arranged by size: 

                1094     731     403     294      52
 
MspA1I CmG'CkG

Cuts at:      0     526    1045    2191    2574 
   Size:        526     519    1146     383

  Fragments arranged by size: 

                1146     526     519     383
 
NcoI C'CATG_G

Cuts at:      0    1458    2574 
   Size:       1458    1116
 
NdeI CA'TA_TG

Cuts at:      0    1956    2061    2574 
   Size:       1956     105     513

  Fragments arranged by size: 

                1956     513     105
 
NspI r_CATG'y

Cuts at:      0    1839    2387    2574 
   Size:       1839     548     187

  Fragments arranged by size: 

                1839     548     187
 
PciI A'CATG_T

Cuts at:      0    1835    2383    2574 
   Size:       1835     548     191

  Fragments arranged by size: 

                1835     548     191
 
PsiI TTA'TAA

Cuts at:      0     551    2574 
   Size:        551    2023
 
PstI C_TGCA'G

Cuts at:      0    2171    2574 
   Size:       2171     403
 
PvuII CAG'CTG

Cuts at:      0     526    1045    2191    2574 
   Size:        526     519    1146     383

  Fragments arranged by size: 

                1146     526     519     383
 
SacI G_AGCT'C

Cuts at:      0     835    2574 
   Size:        835    1739
 
SfcI C'TryA_G

Cuts at:      0    2167    2574 
   Size:       2167     407
 
SmaI CCC'GGG

Cuts at:      0      55    2574 
   Size:         55    2519
 
SmlI C'TyrA_G

Cuts at:      0     258     397    2574 
   Size:        258     139    2177

  Fragments arranged by size: 

                2177     258     139
 
SrfI GCCC'GGGC

Cuts at:      0      55    2574 
   Size:         55    2519
 
SspI AAT'ATT

Cuts at:      0    2460    2574 
   Size:       2460     114
 
StyI C'CwwG_G

Cuts at:      0    1347    1458    2574 
   Size:       1347     111    1116

  Fragments arranged by size: 

                1347    1116     111
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     307    1471    2442    2574 
   Size:        307    1164     971     132

  Fragments arranged by size: 

                1164     971     307     132
 
TatI w'GTAC_w

Cuts at:      0     311     752    2574 
   Size:        311     441    1822

  Fragments arranged by size: 

                1822     441     311
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     834     866    2574 
   Size:        834      32    1708

  Fragments arranged by size: 

                1708     834      32
 
XbaI T'CTAG_A

Cuts at:      0    1426    2574 
   Size:       1426    1148
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1767    2574 
   Size:       1767     807
 
XmaI C'CCGG_G

Cuts at:      0      53    2574 
   Size:         53    2521
 
XmnI GAAnn'nnTTC

Cuts at:      0    1244    2574 
   Size:       1244    1330
 

 Enzymes that do cut:

     AcuI   AflIII     AgeI     AlfI    AlwNI    ApaLI     ApoI     AseI
     AvaI     BaeI    BamHI    BanII     BbsI     BclI Bme1580I    BmgBI
     BmrI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BsrFI    BsrGI    BstYI     BtgI    BtgZI    CspCI     DraI
     EaeI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII
    Hin4I   HincII  HindIII     MmeI     MslI   MspA1I     NcoI     NdeI
     NspI     PciI     PsiI     PstI    PvuII     SacI     SfcI     SmaI
     SmlI     SrfI     SspI     StyI    TaqII     TatI     TstI     XbaI
     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AhdI
     AleI     AloI     ApaI     AscI    AsiSI    AvrII     BanI     BbeI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI     BmtI
     BplI    BsaHI    BseYI    BsiWI    BsmBI    BspMI    BsrBI    BsrDI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
     BtsI     ClaI   DraIII     DrdI     EagI     EarI     EciI    EcoRV
     FseI     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     StuI     SwaI  Tth111I     XhoI
     ZraI
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