Restriction map for pF1KSDA0199
##################################
# NO CUT ENZYMES (   79 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AhdI        Aor51HI     AscI        AseI        AsnI        
Asp700I     Asp718I     AspEI       AvrII       BamHI       
BbeI        BciVI       BclI        BfrI        BglII       
BlnI        BsiWI       BspDI       BspLU11I    BsrDI       
BssHII      BstAPI      BstBI       ClaI        CpoI        
DraI        Eam1105I    Eco47III    EcoNI       EcoT22I     
FbaI        FseI        HincII      HindII      HpaI        
KasI        KpnI        KspI        MfeI        MluI        
MunI        NarI        NdeI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SnaBI       SpeI        Sse8387I    SspI        SwaI        
TfiI        XbaI        XhoI        XmnI        


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# ONE CUT ENZYMES (   31 enzymes) #
###################################

AccIII        386  TCCGGA               T^CCGGA
AcsI          166  GAATTC               [GA]^AATT[CT]
ApoI          166  GAATTC               [GA]^AATT[CT]
AspI          919  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII        1616  TGCGCA               TGC^GCA
BsaBI        1581  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         386  TCCGGA               [AT]^CCGG[AT]
BseAI         386  TCCGGA               T^CCGGA
BspEI         386  TCCGGA               T^CCGGA
BspHI        1175  TCATGA               T^CATGA
Bst1107I     1594  GTATAC               GTA^TAC
BstEII       1842  GGTAACC              G^GT[ACGT]ACC
BstPI        1842  GGTAACC              G^GT[ACGT]ACC
BstZ17I      1594  GTATAC               GTA^TAC
DraIII       3214  CACACAGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         1825  GACAGCCCAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I      1842  GGTAACC              G^GT[ACGT]ACC
EcoRI         166  GAATTC               G^AATTC
EcoRV          30  GATATC               GAT^ATC
FspI         1616  TGCGCA               TGC^GCA
HindIII      3612  AAGCTT               A^AGCTT
MamI         1581  GATTGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          386  TCCGGA               T^CCGGA
MseI         2626  TTAA                 T^TAA
NheI         1344  GCTAGC               G^CTAGC
PflFI         919  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
RcaI         1175  TCATGA               T^CATGA
SapI         1905  GCTCTTC              GCTCTTC(1/4)
SmlI         2482  CTTGAG               C^T[CT][GA]AG
Tru9I        2626  TTAA                 T^TAA
Tth111I       919  GACATGGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AatII        1773  GACGTC               GACGT^C
             2113  GACGTC              
Alw44I        820  GTGCAC               G^TGCAC
             3227  GTGCAC              
ApaLI         820  GTGCAC               G^TGCAC
             3227  GTGCAC              
BbrPI         394  CACGTG               CAC^GTG
              817  CACGTG              
BbsI          715  GTCTTC               GAAGAC(2/6)
             1476  GTCTTC              
BbvCI        1388  CCTCAGC              CCTCAGC(-5/-2)
             3117  CCTCAGC             
BpuAI         715  GTCTTC               GAAGAC(2/6)
             1476  GTCTTC              
BsaAI         394  CACGTG               [CT]AC^GT[GA]
              817  CACGTG              
BsmBI        2111  GAGACG               CGTCTC(1/5)
             2290  GAGACG              
BsmI          364  GCATTC               GAATGC(1/-1)
             2432  GCATTC              
BssSI         592  CACGAG               CACGAG(-5/-1)
             3324  CTCGTG              
BstYI         383  AGATCC               [GA]^GATC[CT]
             3746  AGATCC              
MflI          383  AGATCC               [GA]^GATC[CT]
             3746  AGATCC              
NaeI         2006  GCCGGC               GCC^GGC
             2960  GCCGGC              
NgoMIV       2006  GCCGGC               G^CCGGC
             2960  GCCGGC              
NspI         1697  GCATGC               [GA]CATG^[CT]
             2357  GCATGC              
PmaCI         394  CACGTG               CAC^GTG
              817  CACGTG              
PmlI          394  CACGTG               CAC^GTG
              817  CACGTG              
SacI          850  GAGCTC               GAGCT^C
             3793  GAGCTC              
SphI         1697  GCATGC               GCATG^C
             2357  GCATGC              
TaqI          917  TCGA                 T^CGA
             2336  TCGA                
TthHB8I       917  TCGA                 T^CGA
             2336  TCGA                
XhoII         383  AGATCC               [GA]^GATC[CT]
             3746  AGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (  125 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        AlwI        AlwNI       AocI        ApaI        
AspHI       AvaI        AvaII       BalI        BanI        
BanII       BbvI        BcgI        BcnI        BfaI        
BglI        BlpI        BmyI        BpmI        Bpu1102I    
BsaHI       BsaI        BsaJI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    Bsp1407I    BspMI       
BsrBI       BsrFI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstXI       Bsu36I      
Cac8I       CelII       CfoI        Cfr10I      Cfr13I      
DdeI        DpnII       DraII       DsaI        EaeI        
EagI        EarI        EclXI       Eco52I      Eco81I      
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeII       
MaeIII      MboI        MboII       MluNI       MnlI        
MscI        MslI        MspA1I      MspI        MvaI        
MvnI        MwoI        NciI        NcoI        NdeII       
NlaIII      NlaIV       PflMI       PleI        PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        SmaI        SspBI       
StuI        StyI        TseI        Tsp45I      Tsp509I     
TspRI       Van91I      XcmI        XmaCI       XmaI        
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