Restriction map for pF1KSDA0199
 (Linear) (Six-base) MAPSORT of: KIAA0199.seq  Check: 1718  from: 1  to: 3837



KIAA0199.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2629    3837 
   Size:       2629    1208
 
AatII G_ACGT'C

Cuts at:      0    1777    2117    3837 
   Size:       1777     340    1720

  Fragments arranged by size: 

                1777    1720     340
 
AccI GT'mk_AC

Cuts at:      0    1118    1520    1595    3707    3837 
   Size:       1118     402      75    2112     130

  Fragments arranged by size: 

                2112    1118     402     130      75
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     599    1276    1893    2718    3328    3837 
   Size:        599     677     617     825     610     509

  Fragments arranged by size: 

                 825     677     617     610     599     509
 
AleI CACnn'nnGTG

Cuts at:      0     178    1228    3370    3837 
   Size:        178    1050    2142     467

  Fragments arranged by size: 

                2142    1050     467     178
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     746    3837 
   Size:        746    3091
 
AlwNI CAG_nnn'CTG

Cuts at:      0     160    1299    2033    3551    3597    3750    3837 
   Size:        160    1139     734    1518      46     153      87

  Fragments arranged by size: 

                1518    1139     734     160     153      87      46
 
ApaI G_GGCC'C

Cuts at:      0    2051    2089    2234    2581    2889    3837 
   Size:       2051      38     145     347     308     948

  Fragments arranged by size: 

                2051     948     347     308     145      38
 
ApaLI G'TGCA_C

Cuts at:      0     820    3227    3837 
   Size:        820    2407     610

  Fragments arranged by size: 

                2407     820     610
 
ApoI r'AATT_y

Cuts at:      0     166    3837 
   Size:        166    3671
 
AvaI C'yCGr_G

Cuts at:      0      35     359    1986    2233    2287    2573    2677    2711 
   Size:         35     324    1627     247      54     286     104      34

Cuts at:   2711    2857    3837 
   Size:        146     980

  Fragments arranged by size: 

                1627     980     324     286     247     146     104      54
                  35      34
 
BanI G'GyrC_C

Cuts at:      0     259    1567    2009    2687    3837 
   Size:        259    1308     442     678    1150

  Fragments arranged by size: 

                1308    1150     678     442     259
 
BanII G_rGCy'C

Cuts at:      0      59     854    1654    1671    2051    2089    2234    2581 
   Size:         59     795     800      17     380      38     145     347

Cuts at:   2581    2684    2889    3797    3837 
   Size:        103     205     908      40

  Fragments arranged by size: 

                 908     800     795     380     347     205     145     103
                  59      40      38      17
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     708    1469    3837 
   Size:        708     761    2368

  Fragments arranged by size: 

                2368     761     708
 
BbvCI CC'TCA_GC

Cuts at:      0    1389    3118    3837 
   Size:       1389    1729     719

  Fragments arranged by size: 

                1729    1389     719
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     990    1024    1608    1642    2919    2953    3837 
   Size:        990      34     584      34    1277      34     884

  Fragments arranged by size: 

                1277     990     884     584      34      34      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     268    2238    2384    2576    3837 
   Size:        268    1970     146     192    1261

  Fragments arranged by size: 

                1970    1261     268     192     146
 
BlpI GC'TnA_GC

Cuts at:      0     848     970    3796    3837 
   Size:        848     122    2826      41

  Fragments arranged by size: 

                2826     848     122      41
 
Bme1580I G_kGCm'C

Cuts at:      0     264     759     824    1235    2051    2066    2089    2234 
   Size:        264     495      65     411     816      15      23     145

Cuts at:   2234    2581    2889    3224    3231    3815    3837 
   Size:        347     308     335       7     584      22

  Fragments arranged by size: 

                 816     584     495     411     347     335     308     264
                 145      65      23      22      15       7
 
BmgBI CAC'GTC

Cuts at:      0    2391    3837 
   Size:       2391    1446
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     552    1825    1960    2076    2885    3290    3837 
   Size:        552    1273     135     116     809     405     547

  Fragments arranged by size: 

                1273     809     552     547     405     135     116
 
BmtI G_CTAG'C

Cuts at:      0    1348    3837 
   Size:       1348    2489
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2012    2868    2883    3033    3036    3837 
   Size:       2012     856      15     150       3     801

  Fragments arranged by size: 

                2012     856     801     150      15       3
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1144    2020    2931    2986    3155    3337    3837 
   Size:       1144     876     911      55     169     182     500

  Fragments arranged by size: 

                1144     911     876     500     182     169      55
 
Bpu10I CC'TnA_GC

Cuts at:      0     478     494    1153    1389    1754    1793    2489    3118 
   Size:        478      16     659     236     365      39     696     629

Cuts at:   3118    3837 
   Size:        719

  Fragments arranged by size: 

                 719     696     659     629     478     365     236      39
                  16
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    2503    3837 
   Size:       2503    1334
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     699    1110    3021    3097    3837 
   Size:        699     411    1911      76     740

  Fragments arranged by size: 

                1911     740     699     411      76
 
BsaAI yAC'GTr

Cuts at:      0     396     819    3837 
   Size:        396     423    3018

  Fragments arranged by size: 

                3018     423     396
 
BsaBI GATnn'nnATC

Cuts at:      0    1585    3837 
   Size:       1585    2252
 
BsaHI Gr'CG_yC

Cuts at:      0    1018    1774    2114    2978    3837 
   Size:       1018     756     340     864     859

  Fragments arranged by size: 

                1018     864     859     756     340
 
BsaWI w'CCGG_w

Cuts at:      0     386    3837 
   Size:        386    3451
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     421     451    3310    3340    3837 
   Size:        421      30    2859      30     497

  Fragments arranged by size: 

                2859     497     421      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      49     394     445     847    2551    2779    3009    3025 
   Size:         49     345      51     402    1704     228     230      16

Cuts at:   3025    3837 
   Size:        812

  Fragments arranged by size: 

                1704     812     402     345     230     228      51      49
                  16
 
BseYI C'CCAG_C

Cuts at:      0     778    1197    1605    1708    2059    2139    2220    2498 
   Size:        778     419     408     103     351      80      81     278

Cuts at:   2498    3265    3731    3837 
   Size:        767     466     106

  Fragments arranged by size: 

                 778     767     466     419     408     351     278     106
                 103      81      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     757     984    2119    2767    2769    3050    3837 
   Size:        757     227    1135     648       2     281     787

  Fragments arranged by size: 

                1135     787     757     648     281     227       2
 
BsiEI CG_ry'CG

Cuts at:      0    2025    2780    2961    3371    3837 
   Size:       2025     755     181     410     466

  Fragments arranged by size: 

                2025     755     466     410     181
 
BsiHKAI G_wGCw'C

Cuts at:      0     824     854     971    1091    3231    3797    3837 
   Size:        824      30     117     120    2140     566      40

  Fragments arranged by size: 

                2140     824     566     120     117      40      30
 
BsmI GAATG_Cn'

Cuts at:      0     364    2432    3837 
   Size:        364    2068    1405

  Fragments arranged by size: 

                2068    1405     364
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2105    2284    3837 
   Size:       2105     179    1553

  Fragments arranged by size: 

                2105    1553     179
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     264     759     824     854     971    1091    1235 
   Size:         59     205     495      65      30     117     120     144

Cuts at:   1235    1654    1671    2051    2066    2089    2234    2581    2684 
   Size:        419      17     380      15      23     145     347     103

Cuts at:   2684    2889    3224    3231    3797    3815    3837 
   Size:        205     335       7     566      18      22

  Fragments arranged by size: 

                 566     495     419     380     347     335     205     205
                 145     144     120     117     103      65      59      30
                  23      22      18      17      15       7
 
BspEI T'CCGG_A

Cuts at:      0     386    3837 
   Size:        386    3451
 
BspHI T'CATG_A

Cuts at:      0    1175    3837 
   Size:       1175    2662
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     464    1499    2064    2629    3644    3837 
   Size:        464    1035     565     565    1015     193

  Fragments arranged by size: 

                1035    1015     565     565     464     193
 
BsrBI CCG'CTC

Cuts at:      0    1425    2708    3034    3837 
   Size:       1425    1283     326     803

  Fragments arranged by size: 

                1425    1283     803     326
 
BsrFI r'CCGG_y

Cuts at:      0    2006    2152    2817    2960    3837 
   Size:       2006     146     665     143     877

  Fragments arranged by size: 

                2006     877     665     146     143
 
BsrGI T'GTAC_A

Cuts at:      0    2123    3176    3374    3837 
   Size:       2123    1053     198     463

  Fragments arranged by size: 

                2123    1053     463     198
 
BssSI C'ACGA_G

Cuts at:      0     592    3324    3837 
   Size:        592    2732     513

  Fragments arranged by size: 

                2732     592     513
 
BstEII G'GTnAC_C

Cuts at:      0    1842    3837 
   Size:       1842    1995
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     711     741    1101    1197    1401    3396    3837 
   Size:        711      30     360      96     204    1995     441

  Fragments arranged by size: 

                1995     711     441     360     204      96      30
 
BstYI r'GATC_y

Cuts at:      0     383    3746    3837 
   Size:        383    3363      91

  Fragments arranged by size: 

                3363     383      91
 
BstZ17I GTA'TAC

Cuts at:      0    1596    3837 
   Size:       1596    2241
 
Bsu36I CC'TnA_GG

Cuts at:      0     469    1373    1856    2015    2837    3021    3837 
   Size:        469     904     483     159     822     184     816

  Fragments arranged by size: 

                 904     822     816     483     469     184     159
 
BtgI C'CryG_G

Cuts at:      0      51     956    1190    2106    3389    3477    3837 
   Size:         51     905     234     916    1283      88     360

  Fragments arranged by size: 

                1283     916     905     360     234      88      51
 
BtsI GCAGTG_nn'

Cuts at:      0     127    2034    3110    3531    3795    3837 
   Size:        127    1907    1076     421     264      42

  Fragments arranged by size: 

                1907    1076     421     264     127      42
 
DraIII CAC_nnn'GTG

Cuts at:      0    3219    3837 
   Size:       3219     618
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1831    3837 
   Size:       1831    2006
 
EaeI y'GGCC_r

Cuts at:      0    1187    1897    1931    2022    2147    2319    2345    2703 
   Size:       1187     710      34      91     125     172      26     358

Cuts at:   2703    2777    2958    3398    3651    3837 
   Size:         74     181     440     253     186

  Fragments arranged by size: 

                1187     710     440     358     253     186     181     172
                 125      91      74      34      26
 
EagI C'GGCC_G

Cuts at:      0    2022    2777    2958    3837 
   Size:       2022     755     181     879

  Fragments arranged by size: 

                2022     879     755     181
 
EarI CTCTTCn'nnn_

Cuts at:      0      97     679    1012    1912    2602    3340    3837 
   Size:         97     582     333     900     690     738     497

  Fragments arranged by size: 

                 900     738     690     582     497     333      97
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     817    1879    1966    2256    2573    2964    3837 
   Size:        817    1062      87     290     317     391     873

  Fragments arranged by size: 

                1062     873     817     391     317     290      87
 
EcoICRI GAG'CTC

Cuts at:      0     852    3795    3837 
   Size:        852    2943      42

  Fragments arranged by size: 

                2943     852      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     599    1144    1276    1893    2020    2718    2931    2986 
   Size:        599     545     132     617     127     698     213      55

Cuts at:   2986    3155    3328    3337    3837 
   Size:        169     173       9     500

  Fragments arranged by size: 

                 698     617     599     545     500     213     173     169
                 132     127      55       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0     157    1852    2047    2077    2085    2577    2578    2832 
   Size:        157    1695     195      30       8     492       1     254

Cuts at:   2832    2886    3140    3594    3675    3690    3837 
   Size:         54     254     454      81      15     147

  Fragments arranged by size: 

                1695     492     454     254     254     195     157     147
                  81      54      30      15       8       1
 
EcoRI G'AATT_C

Cuts at:      0     166    3837 
   Size:        166    3671
 
EcoRV GAT'ATC

Cuts at:      0      32    3837 
   Size:         32    3805
 
FspI TGC'GCA

Cuts at:      0    1618    3837 
   Size:       1618    2219
 
HaeII r_GCGC'y

Cuts at:      0    1554    2140    2457    3273    3837 
   Size:       1554     586     317     816     564

  Fragments arranged by size: 

                1554     816     586     564     317
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      16      48    1153    1185    3079    3111    3475    3507 
   Size:         16      32    1105      32    1894      32     364      32

Cuts at:   3507    3837 
   Size:        330

  Fragments arranged by size: 

                1894    1105     364     330      32      32      32      32
                  16
 
HindIII A'AGCT_T

Cuts at:      0    3612    3837 
   Size:       3612     225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     393    3837 
   Size:        393    3444
 
MscI TGG'CCA

Cuts at:      0    1189    1933    2149    2347    3400    3837 
   Size:       1189     744     216     198    1053     437

  Fragments arranged by size: 

                1189    1053     744     437     216     198
 
MslI CAynn'nnrTG

Cuts at:      0     178     728     988    1228    1684    2941    3370    3837 
   Size:        178     550     260     240     456    1257     429     467

  Fragments arranged by size: 

                1257     550     467     456     429     260     240     178
            
 
MspA1I CmG'CkG

Cuts at:      0     662    1431    2220    2477    2738    2751    2957    3262 
   Size:        662     769     789     257     261      13     206     305

Cuts at:   3262    3837 
   Size:        575

  Fragments arranged by size: 

                 789     769     662     575     305     261     257     206
                  13
 
NaeI GCC'GGC

Cuts at:      0    2008    2962    3837 
   Size:       2008     954     875

  Fragments arranged by size: 

                2008     954     875
 
NcoI C'CATG_G

Cuts at:      0      51    2106    3389    3837 
   Size:         51    2055    1283     448

  Fragments arranged by size: 

                2055    1283     448      51
 
NgoMIV G'CCGG_C

Cuts at:      0    2006    2960    3837 
   Size:       2006     954     877

  Fragments arranged by size: 

                2006     954     877
 
NheI G'CTAG_C

Cuts at:      0    1344    3837 
   Size:       1344    2493
 
NspI r_CATG'y

Cuts at:      0    1701    2361    3837 
   Size:       1701     660    1476

  Fragments arranged by size: 

                1701    1476     660
 
PasI CC'CwG_GG

Cuts at:      0     520    3145    3837 
   Size:        520    2625     692

  Fragments arranged by size: 

                2625     692     520
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     375    2328    3390    3750    3837 
   Size:        375    1953    1062     360      87

  Fragments arranged by size: 

                1953    1062     375     360      87
 
PfoI T'CCnGG_A

Cuts at:      0    1241    3837 
   Size:       1241    2596
 
PmlI CAC'GTG

Cuts at:      0     396     819    3837 
   Size:        396     423    3018

  Fragments arranged by size: 

                3018     423     396
 
PpuMI rG'GwC_Cy

Cuts at:      0     157    2077    3140    3594    3675    3690    3837 
   Size:        157    1920    1063     454      81      15     147

  Fragments arranged by size: 

                1920    1063     454     157     147      81      15
 
PspOMI G'GGCC_C

Cuts at:      0    2047    2085    2230    2577    2885    3837 
   Size:       2047      38     145     347     308     952

  Fragments arranged by size: 

                2047     952     347     308     145      38
 
PstI C_TGCA'G

Cuts at:      0     611     793    2927    3004    3131    3837 
   Size:        611     182    2134      77     127     706

  Fragments arranged by size: 

                2134     706     611     182     127      77
 
PvuII CAG'CTG

Cuts at:      0    1431    2220    2738    3837 
   Size:       1431     789     518    1099

  Fragments arranged by size: 

                1431    1099     789     518
 
SacI G_AGCT'C

Cuts at:      0     854    3797    3837 
   Size:        854    2943      40

  Fragments arranged by size: 

                2943     854      40
 
SanDI GG'GwC_CC

Cuts at:      0    3140    3837 
   Size:       3140     697
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1912    3837 
   Size:       1912    1925
 
SfcI C'TryA_G

Cuts at:      0     607     789    2923    3000    3127    3837 
   Size:        607     182    2134      77     127     710

  Fragments arranged by size: 

                2134     710     607     182     127      77
 
SmaI CCC'GGG

Cuts at:      0     361    2235    2575    3837 
   Size:        361    1874     340    1262

  Fragments arranged by size: 

                1874    1262     361     340
 
SmlI C'TyrA_G

Cuts at:      0    2482    3837 
   Size:       2482    1355
 
SphI G_CATG'C

Cuts at:      0    1701    2361    3837 
   Size:       1701     660    1476

  Fragments arranged by size: 

                1701    1476     660
 
SrfI GCCC'GGGC

Cuts at:      0    2235    3837 
   Size:       2235    1602
 
StuI AGG'CCT

Cuts at:      0     467    1151    1378    3837 
   Size:        467     684     227    2459

  Fragments arranged by size: 

                2459     684     467     227
 
StyI C'CwwG_G

Cuts at:      0      51    1146    2081    2106    3098    3389    3837 
   Size:         51    1095     935      25     992     291     448

  Fragments arranged by size: 

                1095     992     935     448     291      51      25
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     247     466    1648    2212    2645    3607    3837 
   Size:        247     219    1182     564     433     962     230

  Fragments arranged by size: 

                1182     962     564     433     247     230     219
 
TatI w'GTAC_w

Cuts at:      0     656    2123    3176    3374    3438    3837 
   Size:        656    1467    1053     198      64     399

  Fragments arranged by size: 

                1467    1053     656     399     198      64
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     421     453    2323    2355    3310    3342    3708    3740 
   Size:        421      32    1870      32     955      32     366      32

Cuts at:   3740    3837 
   Size:         97

  Fragments arranged by size: 

                1870     955     421     366      97      32      32      32
                  32
 
Tth111I GACn'n_nGTC

Cuts at:      0     922    3837 
   Size:        922    2915
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     687     939    1844    2913    3837 
   Size:        687     252     905    1069     924

  Fragments arranged by size: 

                1069     924     905     687     252
 
XmaI C'CCGG_G

Cuts at:      0     359    2233    2573    3837 
   Size:        359    1874     340    1264

  Fragments arranged by size: 

                1874    1264     359     340
 
ZraI GAC'GTC

Cuts at:      0    1775    2115    3837 
   Size:       1775     340    1722

  Fragments arranged by size: 

                1775    1722     340
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI     AleI     AlfI    AlwNI     ApaI
    ApaLI     ApoI     AvaI     BanI    BanII     BbsI    BbvCI     BcgI
     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI    BssSI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI
    EcoRV     FspI    HaeII    Hin4I  HindIII     MmeI     MscI     MslI
   MspA1I     NaeI     NcoI   NgoMIV     NheI     NspI     PasI    PflMI
     PfoI     PmlI    PpuMI   PspOMI     PstI    PvuII     SacI    SanDI
     SapI     SfcI     SmaI     SmlI     SphI     SrfI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AloI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BciVI
     BclI    BfrBI    BglII    BsiWI    BsrDI   BssHII   BstAPI    BstBI
    BtgZI     ClaI    CspCI     DraI    EcoNI     FalI     FseI    FspAI
   HincII     HpaI     KasI     KpnI     MfeI     MluI     NarI     NdeI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PpiI    PshAI
     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SspI     SwaI     XbaI     XhoI     XmnI
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