Restriction map for pF1KSDA0172
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

Acc65I      AccI        AccIII      AcsI        AgeI        
AhdI        Aor51HI     ApoI        AscI        Asp700I     
Asp718I     AspEI       AviII       BamHI       BbeI        
BcgI        BclI        BlpI        Bpu1102I    BsaBI       
BsaWI       BseAI       BsiEI       BsiWI       BspDI       
BspEI       BspLU11I    BsrBI       BssHII      Bst1107I    
BstBI       BstZ17I     CelII       ClaI        CpoI        
DraI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoRV       FbaI        
FseI        FspI        HaeII       KasI        KpnI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PvuI        
RsrII       SacII       SalI        SbfI        ScaI        
SfiI        SfoI        SfuI        SgrAI       SnaBI       
SpeI        Sse8387I    SwaI        XbaI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AatII        4020  GACGTC               GACGT^C
AclI         2217  AACGTT               AA^CGTT
AcyI         4020  GACGTC               G[GA]^CG[CT]C
AflII        2751  CTTAAG               C^TTAAG
Alw44I       2038  GTGCAC               G^TGCAC
AocI           32  CCTCAGG              CC^T[ACGT]AGG
ApaI         1010  GGGCCC               GGGCC^C
ApaLI        2038  GTGCAC               G^TGCAC
AseI         2995  ATTAAT               AT^TAAT
AsnI         2995  ATTAAT               AT^TAAT
AvrII        4003  CCTAGG               C^CTAGG
BbvCI        2652  GCTGAGG              CCTCAGC(-5/-2)
BfrI         2751  CTTAAG               C^TTAAG
BlnI         4003  CCTAGG               C^CTAGG
BsaHI        4020  GACGTC               G[GA]^CG[CT]C
BsmI         3678  GAATGC               GAATGC(1/-1)
Bsp1407I     2899  TGTACA               T^GTACA
BsrDI         804  CATTGC               GCAATG(2/0)
BsrFI        1193  GCCGGT               [GA]^CCGG[CT]
BsrGI        2899  TGTACA               T^GTACA
BssSI        3358  CACGAG               CACGAG(-5/-1)
BstEII       3880  GGTCACC              G^GT[ACGT]ACC
BstPI        3880  GGTCACC              G^GT[ACGT]ACC
Bsu36I         32  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1193  GCCGGT               [GA]^CCGG[CT]
DraIII        524  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco81I         32  CCTCAGG              CC^T[ACGT]AGG
EcoO65I      3880  GGTCACC              G^GT[ACGT]ACC
Hin1I        4020  GACGTC               G[GA]^CG[CT]C
HpaI           19  GTTAAC               GTT^AAC
PflMI        2127  CCAGACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI        2995  ATTAAT               AT^TAAT
Psp1406I     2217  AACGTT               AA^CGTT
SexAI        1595  ACCTGGT              A^CC[AT]GGT
SphI         3274  GCATGC               GCATG^C
SspBI        2899  TGTACA               T^GTACA
StuI         1160  AGGCCT               AGG^CCT
Van91I       2127  CCAGACTGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (   23 enzymes) #
###################################

AflIII       1604  ACACGT               A^C[GA][CT]GT
             1606  ACGTGT              
AspI         1231  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2196  GACGCGGTC           
BbrPI        1605  CACGTG               CAC^GTG
             3835  CACGTG              
BglII         869  AGATCT               A^GATCT
             1403  AGATCT              
BsaAI        1605  CACGTG               [CT]AC^GT[GA]
             3835  CACGTG              
BsgI         2082  CTGCAC               GTGCAG(16/14)
             1558  GTGCAG              
BspHI        2627  TCATGA               T^CATGA
             2930  TCATGA              
BstAPI       1643  GCATCTCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             2511  GCAGACACTGC         
BstXI         648  CCAAGGTAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3596  CCATCATGTTGG        
EcoT22I      1729  ATGCAT               ATGCA^T
             2894  ATGCAT              
HindIII      2549  AAGCTT               A^AGCTT
             3311  AAGCTT              
NcoI          701  CCATGG               C^CATGG
             3401  CCATGG              
NsiI         1729  ATGCAT               ATGCA^T
             2894  ATGCAT              
PflFI        1231  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2196  GACGCGGTC           
PmaCI        1605  CACGTG               CAC^GTG
             3835  CACGTG              
PmlI         1605  CACGTG               CAC^GTG
             3835  CACGTG              
RcaI         2627  TCATGA               T^CATGA
             2930  TCATGA              
SapI         2647  GAAGAGC              GCTCTTC(1/4)
             3047  GCTCTTC             
SmaI         1007  CCCGGG               CCC^GGG
             1940  CCCGGG              
SspI         2293  AATATT               AAT^ATT
             3160  AATATT              
Tth111I      1231  GACATCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2196  GACGCGGTC           
XmaCI        1007  CCCGGG               C^CCGGG
             1940  CCCGGG              
XmaI         1007  CCCGGG               C^CCGGG
             1940  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  111 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaI        AvaII       BalI        
BanI        BanII       BbsI        BbvI        BciVI       
BcnI        BfaI        BglI        BmyI        BpmI        
BpuAI       BsaI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmBI       BsmFI       
BsoBI       Bsp1286I    BspMI       BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraII       
DrdI        DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        HinP1I      
HincII      HindII      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MluNI       MnlI        
MscI        MseI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        PpuMI       
PstI        PvuII       RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XcmI        
XhoII       
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