Restriction map for pF1KSDA0172
 (Linear) (Six-base) MAPSORT of: KIAA0172.seq  Check: 7044  from: 1  to: 4056



KIAA0172.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      34     785     828    4056 
   Size:         34     751      43    3228

  Fragments arranged by size: 

                3228     751      43      34
 
AatII G_ACGT'C

Cuts at:      0    4024    4056 
   Size:       4024      32
 
AclI AA'CG_TT

Cuts at:      0    2218    4056 
   Size:       2218    1838
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     619     841    1367    2666    2719    2941    2965    4056 
   Size:        619     222     526    1299      53     222      24    1091

  Fragments arranged by size: 

                1299    1091     619     526     222     222      53      24
            
 
AflII C'TTAA_G

Cuts at:      0    2751    4056 
   Size:       2751    1305
 
AflIII A'CryG_T

Cuts at:      0    1604    1606    4056 
   Size:       1604       2    2450

  Fragments arranged by size: 

                2450    1604       2
 
AleI CACnn'nnGTG

Cuts at:      0     751    4056 
   Size:        751    3305
 
AlwNI CAG_nnn'CTG

Cuts at:      0    1154    1262    1796    2152    2496    2517    4056 
   Size:       1154     108     534     356     344      21    1539

  Fragments arranged by size: 

                1539    1154     534     356     344     108      21
 
ApaI G_GGCC'C

Cuts at:      0    1014    4056 
   Size:       1014    3042
 
ApaLI G'TGCA_C

Cuts at:      0    2038    4056 
   Size:       2038    2018
 
AseI AT'TA_AT

Cuts at:      0    2996    4056 
   Size:       2996    1060
 
AvaI C'yCGr_G

Cuts at:      0    1007    1940    3128    4056 
   Size:       1007     933    1188     928

  Fragments arranged by size: 

                1188    1007     933     928
 
AvrII C'CTAG_G

Cuts at:      0    4003    4056 
   Size:       4003      53
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     636     669    4056 
   Size:        636      33    3387

  Fragments arranged by size: 

                3387     636      33
 
BanI G'GyrC_C

Cuts at:      0     571    1210    3146    3997    4056 
   Size:        571     639    1936     851      59

  Fragments arranged by size: 

                1936     851     639     571      59
 
BanII G_rGCy'C

Cuts at:      0     712     736     769    1014    1175    1370    2581    3802 
   Size:        712      24      33     245     161     195    1211    1221

Cuts at:   3802    4056 
   Size:        254

  Fragments arranged by size: 

                1221    1211     712     254     245     195     161      33
                  24
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     495    2269    3128    4024    4056 
   Size:        495    1774     859     896      32

  Fragments arranged by size: 

                1774     896     859     495      32
 
BbvCI CC'TCA_GC

Cuts at:      0    2653    4056 
   Size:       2653    1403
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     637    2497    3098    4056 
   Size:        637    1860     601     958

  Fragments arranged by size: 

                1860     958     637     601
 
BfrBI ATG'CAT

Cuts at:      0    1731    2896    4056 
   Size:       1731    1165    1160

  Fragments arranged by size: 

                1731    1165    1160
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1516    1987    3769    4056 
   Size:       1516     471    1782     287

  Fragments arranged by size: 

                1782    1516     471     287
 
BglII A'GATC_T

Cuts at:      0     869    1403    4056 
   Size:        869     534    2653

  Fragments arranged by size: 

                2653     869     534
 
Bme1580I G_kGCm'C

Cuts at:      0     310     574    1014    2042    3149    3836    4000    4056 
   Size:        310     264     440    1028    1107     687     164      56

  Fragments arranged by size: 

                1107    1028     687     440     310     264     164      56
            
 
BmgBI CAC'GTC

Cuts at:      0    1908    4056 
   Size:       1908    2148
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     745    3981    4056 
   Size:        745    3236      75

  Fragments arranged by size: 

                3236     745      75
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1182    1953    4056 
   Size:       1182     771    2103

  Fragments arranged by size: 

                2103    1182     771
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     844     850    1150    1239    2443    2837    3337 
   Size:        394     450       6     300      89    1204     394     500

Cuts at:   3337    4056 
   Size:        719

  Fragments arranged by size: 

                1204     719     500     450     394     394     300      89
                   6
 
Bpu10I CC'TnA_GC

Cuts at:      0     730     735    1174    1685    2653    2774    4056 
   Size:        730       5     439     511     968     121    1282

  Fragments arranged by size: 

                1282     968     730     511     439     121       5
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1831    2423    4056 
   Size:       1831     592    1633

  Fragments arranged by size: 

                1831    1633     592
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      56      94     370     472     909    1057    1562    2323 
   Size:         56      38     276     102     437     148     505     761

Cuts at:   2323    4056 
   Size:       1733

  Fragments arranged by size: 

                1733     761     505     437     276     148     102      56
                  38
 
BsaAI yAC'GTr

Cuts at:      0    1607    3837    4056 
   Size:       1607    2230     219

  Fragments arranged by size: 

                2230    1607     219
 
BsaHI Gr'CG_yC

Cuts at:      0    4021    4056 
   Size:       4021      35
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     330     360     422     452    1237    1267    2167    2197 
   Size:        330      30      62      30     785      30     900      30

Cuts at:   2197    2835    2865    3784    3814    4056 
   Size:        638      30     919      30     242

  Fragments arranged by size: 

                 919     900     785     638     330     242      62      30
                  30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     847    1063    1155    1231    1834    2175    3118    3121 
   Size:        847     216      92      76     603     341     943       3

Cuts at:   3121    3124    3127    4056 
   Size:          3       3     929

  Fragments arranged by size: 

                 943     929     847     603     341     216      92      76
                   3       3       3
 
BseYI C'CCAG_C

Cuts at:      0     678     989    1520    1744    2432    2768    3863    4056 
   Size:        678     311     531     224     688     336    1095     193

  Fragments arranged by size: 

                1095     688     678     531     336     311     224     193
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1579    2067    4056 
   Size:       1579     488    1989

  Fragments arranged by size: 

                1989    1579     488
 
BsiHKAI G_wGCw'C

Cuts at:      0     712     736     860    1081    1175    1370    2042    3830 
   Size:        712      24     124     221      94     195     672    1788

Cuts at:   3830    4056 
   Size:        226

  Fragments arranged by size: 

                1788     712     672     226     221     195     124      94
                  24
 
BsmI GAATG_Cn'

Cuts at:      0    3684    4056 
   Size:       3684     372
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    2053    2131    4028    4056 
   Size:       2053      78    1897      28

  Fragments arranged by size: 

                2053    1897      78      28
 
Bsp1286I G_dGCh'C

Cuts at:      0     310     574     712     736     769     860    1014    1081 
   Size:        310     264     138      24      33      91     154      67

Cuts at:   1081    1175    1370    2042    2581    3149    3802    3830    3836 
   Size:         94     195     672     539     568     653      28       6

Cuts at:   3836    4000    4056 
   Size:        164      56

  Fragments arranged by size: 

                 672     653     568     539     310     264     195     164
                 154     138      94      91      67      56      33      28
                  24       6
 
BspHI T'CATG_A

Cuts at:      0    2627    2930    4056 
   Size:       2627     303    1126

  Fragments arranged by size: 

                2627    1126     303
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      34     785     828    3192    4056 
   Size:         34     751      43    2364     864

  Fragments arranged by size: 

                2364     864     751      43      34
 
BsrDI GCAATG_nn'

Cuts at:      0     803    4056 
   Size:        803    3253
 
BsrFI r'CCGG_y

Cuts at:      0    1193    4056 
   Size:       1193    2863
 
BsrGI T'GTAC_A

Cuts at:      0    2899    4056 
   Size:       2899    1157
 
BssSI C'ACGA_G

Cuts at:      0    3358    4056 
   Size:       3358     698
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1649    2517    4056 
   Size:       1649     868    1539

  Fragments arranged by size: 

                1649    1539     868
 
BstEII G'GTnAC_C

Cuts at:      0    3880    4056 
   Size:       3880     176
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     655    3603    4056 
   Size:        655    2948     453

  Fragments arranged by size: 

                2948     655     453
 
BstYI r'GATC_y

Cuts at:      0     740     869    1139    1403    4056 
   Size:        740     129     270     264    2653

  Fragments arranged by size: 

                2653     740     270     264     129
 
Bsu36I CC'TnA_GG

Cuts at:      0      33    4056 
   Size:         33    4023
 
BtgI C'CryG_G

Cuts at:      0     701    1622    3401    3803    4056 
   Size:        701     921    1779     402     253

  Fragments arranged by size: 

                1779     921     701     402     253
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    3930    4056 
   Size:       3930     126
 
BtsI GCAGTG_nn'

Cuts at:      0      33     310    1559    1997    2515    3905    4056 
   Size:         33     277    1249     438     518    1390     151

  Fragments arranged by size: 

                1390    1249     518     438     277     151      33
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     930     965    2336    2371    4056 
   Size:        930      35    1371      35    1685

  Fragments arranged by size: 

                1685    1371     930      35      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     529    4056 
   Size:        529    3527
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1576    1912    2271    4056 
   Size:       1576     336     359    1785

  Fragments arranged by size: 

                1785    1576     359     336
 
EaeI y'GGCC_r

Cuts at:      0     551     800    2063    2229    4056 
   Size:        551     249    1263     166    1827

  Fragments arranged by size: 

                1827    1263     551     249     166
 
EarI CTCTTCn'nnn_

Cuts at:      0     187     881    2642    3054    3095    4056 
   Size:        187     694    1761     412      41     961

  Fragments arranged by size: 

                1761     961     694     412     187      41
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     213     706    4056 
   Size:        213     493    3350

  Fragments arranged by size: 

                3350     493     213
 
EcoICRI GAG'CTC

Cuts at:      0     710     734    1173    1368    4056 
   Size:        710      24     439     195    2688

  Fragments arranged by size: 

                2688     710     439     195      24
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     394     619     841     844     850    1150    1239    1367 
   Size:        394     225     222       3       6     300      89     128

Cuts at:   1367    2443    2666    2719    2837    2941    2965    3337    4056 
   Size:       1076     223      53     118     104      24     372     719

  Fragments arranged by size: 

                1076     719     394     372     300     225     223     222
                 128     118     104      89      53      24       6       3
            
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1259    1616    1681    2730    2789    3753    4056 
   Size:       1259     357      65    1049      59     964     303

  Fragments arranged by size: 

                1259    1049     964     357     303      65      59
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1441    1872    2464    3068    4056 
   Size:       1441     431     592     604     988

  Fragments arranged by size: 

                1441     988     604     592     431
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     170     202    1180    1212    2167    2199    2236    2268 
   Size:        170      32     978      32     955      32      37      32

Cuts at:   2268    3019    3031    3051    3063    3324    3356    3784    3816 
   Size:        751      12      20      12     261      32     428      32

Cuts at:   3816    4056 
   Size:        240

  Fragments arranged by size: 

                 978     955     751     428     261     240     170      37
                  32      32      32      32      32      32      20      12
                  12
 
HincII GTy'rAC

Cuts at:      0      21    1503    1905    2357    3780    3972    4056 
   Size:         21    1482     402     452    1423     192      84

  Fragments arranged by size: 

                1482    1423     452     402     192      84      21
 
HindIII A'AGCT_T

Cuts at:      0    2549    3311    4056 
   Size:       2549     762     745

  Fragments arranged by size: 

                2549     762     745
 
HpaI GTT'AAC

Cuts at:      0      21    4056 
   Size:         21    4035
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     308    1395    2193    3542    4056 
   Size:        308    1087     798    1349     514

  Fragments arranged by size: 

                1349    1087     798     514     308
 
MscI TGG'CCA

Cuts at:      0     553     802    2231    4056 
   Size:        553     249    1429    1825

  Fragments arranged by size: 

                1825    1429     553     249
 
MslI CAynn'nnrTG

Cuts at:      0     517     751     853    1237    1990    4056 
   Size:        517     234     102     384     753    2066

  Fragments arranged by size: 

                2066     753     517     384     234     102
 
MspA1I CmG'CkG

Cuts at:      0     417     682     912     993    1154    1276    2257    2884 
   Size:        417     265     230      81     161     122     981     627

Cuts at:   2884    3619    4056 
   Size:        735     437

  Fragments arranged by size: 

                 981     735     627     437     417     265     230     161
                 122      81
 
NcoI C'CATG_G

Cuts at:      0     701    3401    4056 
   Size:        701    2700     655

  Fragments arranged by size: 

                2700     701     655
 
NsiI A_TGCA'T

Cuts at:      0    1733    2898    4056 
   Size:       1733    1165    1158

  Fragments arranged by size: 

                1733    1165    1158
 
NspI r_CATG'y

Cuts at:      0    1122    2900    3278    3642    4056 
   Size:       1122    1778     378     364     414

  Fragments arranged by size: 

                1778    1122     414     378     364
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2133    4056 
   Size:       2133    1923
 
PfoI T'CCnGG_A

Cuts at:      0    1142    3785    4056 
   Size:       1142    2643     271

  Fragments arranged by size: 

                2643    1142     271
 
PmlI CAC'GTG

Cuts at:      0    1607    3837    4056 
   Size:       1607    2230     219

  Fragments arranged by size: 

                2230    1607     219
 
PpuMI rG'GwC_Cy

Cuts at:      0    1259    1616    2789    4056 
   Size:       1259     357    1173    1267

  Fragments arranged by size: 

                1267    1259    1173     357
 
PspOMI G'GGCC_C

Cuts at:      0    1010    4056 
   Size:       1010    3046
 
PstI C_TGCA'G

Cuts at:      0     782    1478    2007    4056 
   Size:        782     696     529    2049

  Fragments arranged by size: 

                2049     782     696     529
 
PvuII CAG'CTG

Cuts at:      0     417     682     912     993    1154    1276    2257    4056 
   Size:        417     265     230      81     161     122     981    1799

  Fragments arranged by size: 

                1799     981     417     265     230     161     122      81
            
 
SacI G_AGCT'C

Cuts at:      0     712     736    1175    1370    4056 
   Size:        712      24     439     195    2686

  Fragments arranged by size: 

                2686     712     439     195      24
 
SapI GCTCTTCn'nnn_

Cuts at:      0    2642    3054    4056 
   Size:       2642     412    1002

  Fragments arranged by size: 

                2642    1002     412
 
SexAI A'CCwGG_T

Cuts at:      0    1595    4056 
   Size:       1595    2461
 
SfcI C'TryA_G

Cuts at:      0     778     969    1277    1474    2003    2153    2333    2408 
   Size:        778     191     308     197     529     150     180      75

Cuts at:   2408    2532    4056 
   Size:        124    1524

  Fragments arranged by size: 

                1524     778     529     308     197     191     180     150
                 124      75
 
SmaI CCC'GGG

Cuts at:      0    1009    1942    4056 
   Size:       1009     933    2114

  Fragments arranged by size: 

                2114    1009     933
 
SmlI C'TyrA_G

Cuts at:      0    1846    2438    2751    4056 
   Size:       1846     592     313    1305

  Fragments arranged by size: 

                1846    1305     592     313
 
SphI G_CATG'C

Cuts at:      0    3278    4056 
   Size:       3278     778
 
SspI AAT'ATT

Cuts at:      0    2295    3162    4056 
   Size:       2295     867     894

  Fragments arranged by size: 

                2295     894     867
 
StuI AGG'CCT

Cuts at:      0    1162    4056 
   Size:       1162    2894
 
StyI C'CwwG_G

Cuts at:      0     648     701    3401    4003    4056 
   Size:        648      53    2700     602      53

  Fragments arranged by size: 

                2700     648     602      53      53
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1198    1453    1751    2140    4051    4056 
   Size:       1198     255     298     389    1911       5

  Fragments arranged by size: 

                1911    1198     389     298     255       5
 
TatI w'GTAC_w

Cuts at:      0    1419    2899    2914    4056 
   Size:       1419    1480      15    1142

  Fragments arranged by size: 

                1480    1419    1142      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     422     454    1237    1269    4056 
   Size:        422      32     783      32    2787

  Fragments arranged by size: 

                2787     783     422      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1234    2199    4056 
   Size:       1234     965    1857

  Fragments arranged by size: 

                1857    1234     965
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     471     637     699    1572    2881    3399    4056 
   Size:        471     166      62     873    1309     518     657

  Fragments arranged by size: 

                1309     873     657     518     471     166      62
 
XmaI C'CCGG_G

Cuts at:      0    1007    1940    4056 
   Size:       1007     933    2116

  Fragments arranged by size: 

                2116    1007     933
 
ZraI GAC'GTC

Cuts at:      0    4022    4056 
   Size:       4022      34
 

 Enzymes that do cut:

     AarI    AatII     AclI     AcuI    AflII   AflIII     AleI    AlwNI
     ApaI    ApaLI     AseI     AvaI    AvrII     BaeI     BanI    BanII
     BbsI    BbvCI    BciVI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BspMI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII
    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI    CspCI   DraIII
     DrdI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI
    Hin4I   HincII  HindIII     HpaI     MmeI     MscI     MslI   MspA1I
     NcoI     NsiI     NspI    PflMI     PfoI     PmlI    PpuMI   PspOMI
     PstI    PvuII     SacI     SapI    SexAI     SfcI     SmaI     SmlI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI     XmaI     ZraI

 Enzymes that do not cut: 

     AccI   Acc65I     AfeI     AgeI     AhdI     AlfI     AloI     ApoI
     AscI    AsiSI    BamHI     BbeI     BcgI     BclI     BlpI     BmtI
    BsaBI    BsaWI    BsiEI    BsiWI    BspEI    BsrBI   BssHII    BstBI
  BstZ17I     ClaI     DraI     EagI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII     KasI     KpnI     MfeI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI     PmeI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI    SnaBI     SpeI     SrfI     SwaI     XbaI     XhoI
     XmnI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com