Restriction map for pF1KSDA0158
 (Linear) (Six-base) MAPSORT of: KIAA0158.seq  Check: 4226  from: 1  to: 1083



KIAA0158.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:11  ..

Acc65I G'GTAC_C

Cuts at:      0     383    1083 
   Size:        383     700
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     586     604     841    1083 
   Size:        586      18     237     242

  Fragments arranged by size: 

                 586     242     237      18
 
AflII C'TTAA_G

Cuts at:      0     478    1083 
   Size:        478     605
 
AvaI C'yCGr_G

Cuts at:      0    1069    1083 
   Size:       1069      14
 
BamHI G'GATC_C

Cuts at:      0     721    1083 
   Size:        721     362
 
BanI G'GyrC_C

Cuts at:      0     383    1073    1083 
   Size:        383     690      10

  Fragments arranged by size: 

                 690     383      10
 
BanII G_rGCy'C

Cuts at:      0     986    1068    1083 
   Size:        986      82      15

  Fragments arranged by size: 

                 986      82      15
 
BglII A'GATC_T

Cuts at:      0     959    1083 
   Size:        959     124
 
BlpI GC'TnA_GC

Cuts at:      0     980    1083 
   Size:        980     103
 
Bme1580I G_kGCm'C

Cuts at:      0    1076    1083 
   Size:       1076       7
 
BmtI G_CTAG'C

Cuts at:      0     701    1083 
   Size:        701     382
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     784    1083 
   Size:        784     299
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     844    1083 
   Size:        227     617     239

  Fragments arranged by size: 

                 617     239     227
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     676     895    1083 
   Size:        676     219     188

  Fragments arranged by size: 

                 676     219     188
 
BsaAI yAC'GTr

Cuts at:      0    1080    1083 
   Size:       1080       3
 
BsiHKAI G_wGCw'C

Cuts at:      0     809     986    1083 
   Size:        809     177      97

  Fragments arranged by size: 

                 809     177      97
 
Bsp1286I G_dGCh'C

Cuts at:      0     809     986    1068    1076    1083 
   Size:        809     177      82       8       7

  Fragments arranged by size: 

                 809     177      82       8       7
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     395    1083 
   Size:        395     688
 
BsrBI CCG'CTC

Cuts at:      0     401     579    1083 
   Size:        401     178     504

  Fragments arranged by size: 

                 504     401     178
 
BsrDI GCAATG_nn'

Cuts at:      0     539    1083 
   Size:        539     544
 
BstEII G'GTnAC_C

Cuts at:      0     867    1083 
   Size:        867     216
 
BstYI r'GATC_y

Cuts at:      0     721     855     959    1083 
   Size:        721     134     104     124

  Fragments arranged by size: 

                 721     134     124     104
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1070    1083 
   Size:       1070      13
 
DraI TTT'AAA

Cuts at:      0     672    1083 
   Size:        672     411
 
DraIII CAC_nnn'GTG

Cuts at:      0    1080    1083 
   Size:       1080       3
 
EarI CTCTTCn'nnn_

Cuts at:      0     260    1083 
   Size:        260     823
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     976    1083 
   Size:        976     107
 
EcoICRI GAG'CTC

Cuts at:      0     984    1083 
   Size:        984      99
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     227     586     604     841     844    1083 
   Size:        227     359      18     237       3     239

  Fragments arranged by size: 

                 359     239     237     227      18       3
 
EcoO109I rG'GnC_Cy

Cuts at:      0     876    1083 
   Size:        876     207
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     634     666    1083 
   Size:        634      32     417

  Fragments arranged by size: 

                 634     417      32
 
KpnI G_GTAC'C

Cuts at:      0     387    1083 
   Size:        387     696
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      33     699    1083 
   Size:         33     666     384

  Fragments arranged by size: 

                 666     384      33
 
MslI CAynn'nnrTG

Cuts at:      0     118     847    1083 
   Size:        118     729     236

  Fragments arranged by size: 

                 729     236     118
 
NheI G'CTAG_C

Cuts at:      0     697    1083 
   Size:        697     386
 
NspI r_CATG'y

Cuts at:      0     852     996    1083 
   Size:        852     144      87

  Fragments arranged by size: 

                 852     144      87
 
PasI CC'CwG_GG

Cuts at:      0     776    1083 
   Size:        776     307
 
PfoI T'CCnGG_A

Cuts at:      0     860    1083 
   Size:        860     223
 
PmlI CAC'GTG

Cuts at:      0    1080    1083 
   Size:       1080       3
 
PpuMI rG'GwC_Cy

Cuts at:      0     876    1083 
   Size:        876     207
 
PsiI TTA'TAA

Cuts at:      0      28    1083 
   Size:         28    1055
 
PstI C_TGCA'G

Cuts at:      0     334    1083 
   Size:        334     749
 
SacI G_AGCT'C

Cuts at:      0     986    1083 
   Size:        986      97
 
SapI GCTCTTCn'nnn_

Cuts at:      0     260    1083 
   Size:        260     823
 
SfcI C'TryA_G

Cuts at:      0     330    1083 
   Size:        330     753
 
SmlI C'TyrA_G

Cuts at:      0     478     691     910    1083 
   Size:        478     213     219     173

  Fragments arranged by size: 

                 478     219     213     173
 
SphI G_CATG'C

Cuts at:      0     996    1083 
   Size:        996      87
 
SspI AAT'ATT

Cuts at:      0     528    1083 
   Size:        528     555
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     859    1083 
   Size:        859     224
 

 Enzymes that do cut:

   Acc65I     AcuI    AflII     AvaI    BamHI     BanI    BanII    BglII
     BlpI Bme1580I     BmtI     BplI     BpmI    BpuEI    BsaAI  BsiHKAI
 Bsp1286I    BspMI    BsrBI    BsrDI   BstEII    BstYI    BtgZI     DraI
   DraIII     EarI     EciI  EcoICRI  Eco57MI EcoO109I    Hin4I     KpnI
     MmeI     MslI     NheI     NspI     PasI     PfoI     PmlI    PpuMI
     PsiI     PstI     SacI     SapI     SfcI     SmlI     SphI     SspI
    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BmgBI     BmrI   Bpu10I     BsaI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I     BtgI     BtsI
     ClaI    CspCI     DrdI     EaeI     EagI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     PacI     PciI    PflMI
     PmeI     PpiI    PshAI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI
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