Restriction map for pF1KSDA0154
##################################
# NO CUT ENZYMES (   98 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        Alw44I      Aor51HI     
ApaLI       ApoI        AscI        AseI        AsnI        
Asp700I     Asp718I     AspI        AviII       AvrII       
BciVI       BfrI        BglI        BglII       BlnI        
BsaBI       BsaI        BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       BsmI        BspEI       BspHI       
BspLU11I    BsrDI       BsrFI       BssSI       Bst1107I    
BstAPI      BstBI       BstZ17I     Cfr10I      CpoI        
DraIII      DrdI        EagI        EclXI       Eco47III    
Eco52I      EcoRI       EcoRV       EcoT22I     FseI        
FspI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NaeI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PinAI       
PmeI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SalI        ScaI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspI        SwaI        Tth111I     
XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccII        1434  CGCG                 CG^CG
AflIII        548  ACACGT               A^C[GA][CT]GT
BamHI         521  GGATCC               G^GATCC
BbeI         1219  GGCGCC               GGCGC^C
BbrPI        1654  CACGTG               CAC^GTG
BbvCI        1247  CCTCAGC              CCTCAGC(-5/-2)
BcgI          872  GCACGCTCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI         2010  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2010  GCTGAGC              GC^T[ACGT]AGC
BsaAI        1654  CACGTG               [CT]AC^GT[GA]
Bsp1407I      254  TGTACA               T^GTACA
BspDI        1668  ATCGAT               AT^CGAT
BsrGI         254  TGTACA               T^GTACA
BssHII       1433  GCGCGC               G^CGCGC
BstEII        531  GGTGACC              G^GT[ACGT]ACC
BstPI         531  GGTGACC              G^GT[ACGT]ACC
BstUI        1434  CGCG                 CG^CG
BstXI         169  CCACAGGAATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII        2010  GCTGAGC              GC^T[ACGT]AGC
ClaI         1668  ATCGAT               AT^CGAT
DraI          688  TTTAAA               TTT^AAA
EcoNI         468  CCTGCAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I       531  GGTGACC              G^GT[ACGT]ACC
HincII        562  GTTAAC               GT[CT]^[GA]AC
HindII        562  GTTAAC               GT[CT]^[GA]AC
HindIII       427  AAGCTT               A^AGCTT
HpaI          562  GTTAAC               GTT^AAC
KasI         1219  GGCGCC               G^GCGCC
MvnI         1434  CGCG                 CG^CG
NarI         1219  GGCGCC               GG^CGCC
NspI          308  ACATGC               [GA]CATG^[CT]
PmaCI        1654  CACGTG               CAC^GTG
PmlI         1654  CACGTG               CAC^GTG
PpuMI         990  AGGACCT              [GA]G^G[AT]CC[CT]
SacI         1633  GAGCTC               GAGCT^C
SapI         1581  GCTCTTC              GCTCTTC(1/4)
SbfI          468  CCTGCAGG             CCTGCA^GG
SfoI         1219  GGCGCC               GGC^GCC
SmaI          761  CCCGGG               CCC^GGG
SmlI         1260  CTCAAG               C^T[CT][GA]AG
Sse8387I      468  CCTGCAGG             CCTGCA^GG
SspBI         254  TGTACA               T^GTACA
StuI          983  AGGCCT               AGG^CCT
TfiI         1666  GAATC                G^A[AT]TC
XbaI         1467  TCTAGA               T^CTAGA
XmaCI         761  CCCGGG               C^CCGGG
XmaI          761  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcyI          298  GACGCC               G[GA]^CG[CT]C
             1219  GGCGCC              
AfaI          201  GTAC                 GT^AC
              255  GTAC                
AhdI          211  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              534  GACCAAGCGTC         
AocI          950  CCTCAGG              CC^T[ACGT]AGG
              986  CCTCAGG             
ApaI         1036  GGGCCC               GGGCC^C
             1567  GGGCCC              
AspEI         211  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              534  GACCAAGCGTC         
AspHI        1633  GAGCTC               G[AT]GC[AT]^C
             2013  GAGCAC              
AvaI          761  CCCGGG               C^[CT]CG[GA]G
             1488  CTCGGG              
BalI         1643  TGGCCA               TGG^CCA
             1946  TGGCCA              
BbsI          231  GAAGAC               GAAGAC(2/6)
              508  GAAGAC              
BclI           99  TGATCA               T^GATCA
              657  TGATCA              
BpuAI         231  GAAGAC               GAAGAC(2/6)
              508  GAAGAC              
BsaHI         298  GACGCC               G[GA]^CG[CT]C
             1219  GGCGCC              
BsiHKAI      1633  GAGCTC               G[AT]GC[AT]^C
             2013  GAGCAC              
BsoBI         761  CCCGGG               C^[CT]CG[GA]G
             1488  CTCGGG              
BsrBI        1005  CCGCTC               CCGCTC(-3/-3)
             1579  CCGCTC              
BstYI         158  AGATCC               [GA]^GATC[CT]
              521  GGATCC              
Bsu36I        950  CCTCAGG              CC^T[ACGT]AGG
              986  CCTCAGG             
EaeI         1643  TGGCCA               [CT]^GGCC[GA]
             1946  TGGCCA              
Eam1105I      211  GACAGGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
              534  GACCAAGCGTC         
Eco81I        950  CCTCAGG              CC^T[ACGT]AGG
              986  CCTCAGG             
FbaI           99  TGATCA               T^GATCA
              657  TGATCA              
HaeII         183  GGCGCT               [GA]GCGC^[CT]
             1219  GGCGCC              
Hin1I         298  GACGCC               G[GA]^CG[CT]C
             1219  GGCGCC              
MflI          158  AGATCC               [GA]^GATC[CT]
              521  GGATCC              
MluNI        1643  TGGCCA               TGG^CCA
             1946  TGGCCA              
MscI         1643  TGGCCA               TGG^CCA
             1946  TGGCCA              
PflMI        1410  CCATGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1873  CCACCTTCTGG         
RsaI          201  GTAC                 GT^AC
              255  GTAC                
Van91I       1410  CCATGGCTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1873  CCACCTTCTGG         
XhoII         158  AGATCC               [GA]^GATC[CT]
              521  GGATCC              


#########################################
# 3 OR MORE CUT ENZYMES (   82 enzymes) #
#########################################

AciI        AluI        AlwI        AlwNI       AvaII       
BanI        BanII       BbvI        BcnI        BfaI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EarI        EcoO109I    EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeIII      
HapII       HgaI        HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MnlI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PstI        PvuII       Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StyI        
TaqI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       TthHB8I     
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com